Dynamics of an enzymatic substitution reaction in haloalkane dehalogenase

Nam, K.; Prat-Resina, X.; Garcia-Viloca, M.; Devi-Kesavan, L.S.; Gao, J.; J. Am. Chem. Soc. 126, 1369-1376 (2004)

 show all sequences of 3.8.1.5

Data extracted from this reference:

Crystallization (Commentary)
Crystallization Organism
enzyme-substrate-complex: molecular dynamics simulation and structure modeling on the basis of X-ray diffraction data at 2.4 A resolution, overview Xanthobacter autotrophicus
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
additional information
-
 additional information
-
 Xanthobacter autotrophicus
-
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Xanthobacter autotrophicus P22643
-
-
Reaction
Reaction Commentary Organism
1-haloalkane + H2O = a primary alcohol + halide
-
 Xanthobacter autotrophicus
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
1,2-dichloroethane + H2O
-
 655788 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
-
-
 ?
Subunits
Subunits Commentary Organism
More structure modeling of the enzyme-substrate-complex on the basis of X-ray diffraction data at 2.4 A resolution, overview Xanthobacter autotrophicus
Crystallization (Commentary) (protein specific)
Crystallization Organism
enzyme-substrate-complex: molecular dynamics simulation and structure modeling on the basis of X-ray diffraction data at 2.4 A resolution, overview Xanthobacter autotrophicus
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
additional information
-
 additional information
-
 Xanthobacter autotrophicus
-
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
1,2-dichloroethane + H2O
-
 655788 Xanthobacter autotrophicus 2-chloroethanol + chloride
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
More structure modeling of the enzyme-substrate-complex on the basis of X-ray diffraction data at 2.4 A resolution, overview Xanthobacter autotrophicus

See also following references to EC number 3.8.1.5 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
695378PrudnikovaCrystallization and preliminar ...Bradyrhizobium elkaniiActa Crystallogr. Sect. F65353-3562009--11------11-4--1-----1--------------1-1--------11---1----1---------------
698493JesenskaNanosecond time-dependent Stok ...Bradyrhizobium japonicum, Rhodococcus rhodochrousJ. Am. Chem. Soc.131494-5012009--2----------5--2--------------------2---------------2--------------------
699773Murugan1,2-dichloroethane in haloalka ...Xanthobacter autotrophicusJ. Phys. Chem. B1133257-32632009-------------2--------1-----------------------------------1---------------
685394MazumdarX-ray crystal structure of Myc ...Mycobacterium tuberculosis H37RvBiochim. Biophys. Acta1784351-3622008-111-------1-3--1-----3-------------11-1---------1---1----3---------------
689941OtyepkaSecond step of hydrolytic deha ...Sphingomonas paucimobilisProteins70707-7172008-1---------1-3--------1-------------1------------1--------1---------------
695343StsiapanavaCrystals of DhaA mutants from ...Rhodococcus rhodochrousActa Crystallogr. Sect. F64137-1402008--11---------3--1-----1--------------1-1-------------1----1---------------
698415RainaNew metabolites in the degrada ...Sphingobium indicumJ. Agric. Food Chem.566594-66032008-------------4--------8-----------------------------------8---------------
700803Mena-BenitezEngineering a catabolic pathwa ...Xanthobacter autotrophicusPlant Physiol.1471192-11982008--1----------4--------1--------------1--------------------1---------------
684172SatoCrystallization and preliminar ...Bradyrhizobium japonicumActa Crystallogr. Sect. F63294-2962007-111-------1-3--1-----2-------------11-1---------1---1----2---------------
684553MonincovaWeak activity of haloalkane de ...Sphingobium japonicumAppl. Environ. Microbiol.732005-20082007--11---1---1-3--1-----2-1--11--------1-1-----1---1---1----2-1--11---------
684753ItoDegradation of beta-hexachloro ...Sphingobium japonicum, Sphingobium sp.Arch. Microbiol.188313-3252007--2-6------3-8--2---2-3-2---2--------2--6--------3---2--2-3-2---2---------
685187SilbersteinExploring the binding sites of ...Rhodococcus sp., Sphingomonas paucimobilis, Xanthobacter autotrophicusBiochemistry469239-92492007-3---------3-5--------3-------------3------------3--------3---------------
688462NegriStepwise dissection and visual ...Sphingobium japonicumJ. Mol. Graph. Model.26643-6512007-1----1------2------1-4-------------1------1------------1-4---------------
689927ChovancovaPhylogenetic analysis of haloa ...Agrobacterium tumefaciens, Bradyrhizobium japonicum, Mesorhizobium loti, Mycobacterium avium, Mycobacterium smegmatis, Mycobacterium tuberculosis, Rhodococcus sp., Rhodopirellula baltica, Sphingobium japonicum, Xanthobacter autotrophicusProteins67305-3162007-----------12-10--------12--------------------------12--------12---------------
667269SharmaHaloalkane dehalogenase LinB i ...Sphingobium francense, Sphingobium indicum, Sphingobium japonicumAppl. Environ. Microbiol.725720-57272006--3-------34-10--3-----12-3---3--------3----------34---3----12-3---3---------
667851TorzMetabolism of mono- and dihalo ...Xanthobacter autotrophicusBiodegradation18145-1572006-------------2--------6-----------------------------------6---------------
669639BanasMechanism of enhanced conversi ...Rhodococcus sp.J. Comp.-Aided Mol. Des.20375-3832006----1------1-4--------2-----------------2--------1--------2---------------
670049PavlovaThe identification of catalyti ...Mycobacterium aviumJ. Struct. Biol.157384-3922006--1-5-----11-6--11--2-4-1---1--------1--10-------11---1--2-4-1---1---------
670787NakamuraExpression of glycosylated hal ...Sphingomonas paucimobilisProtein Expr. Purif.4685-912006--------1----7-11---2-21--2-------------------1-----11--2-21--2-----------
667252NagataDegradation of beta-hexachloro ...Sphingomonas paucimobilisAppl. Environ. Microbiol.712183-21852005-------------3--------2-----------------------------------2---------------
667258SatoTwo rhizobial strains, Mesorhi ...Bradyrhizobium japonicum, Mesorhizobium lotiAppl. Environ. Microbiol.714372-43792005--1-3-28-----7--1-----21----8----2----1--3--228-------1----21----8----------
667261JesenskaCloning, biochemical propertie ...Mycobacterium bovisAppl. Environ. Microbiol.716736-67452005--1---12-----8--1-----312-122--------1-----1-2-------1----312-122---------
667656KmunicekQuantitative analysis of subst ...Sphingomonas paucimobilisBiochemistry443390-34012005-------11-----3--------12----13-----------------11------------12----13----------
668038ErableNonconventional hydrolytic deh ...Rhodococcus erythropolis, Xanthobacter autotrophicusBiotechnol. Bioeng.91304-3132005-2-----2-----3------2-2-------------2--------2----------2-2---------------
669048SorianoDynamic and electrostatic effe ...Xanthobacter autotrophicusJ. Am. Chem. Soc.1271946-19572005-------------1---1----1-----------------------------------1---------------
670729Erable-Biotransformation of halogenat ...Rhodococcus erythropolisProcess Biochem.4045-512005-1-----1-----1---1----1-1-----------1--------1------------1-1-------------
654762OakleyCrystal structure of haloalkan ...Sphingomonas paucimobilisBiochemistry43870-8782004---1---------2---1----12---------------1------------------12--------------
655788NamDynamics of an enzymatic subst ...Xanthobacter autotrophicusJ. Am. Chem. Soc.1261369-13762004---1---1-----2---1----11---------------1-----1------------11--------------
668037ErableHaloalkane hydrolysis by Rhodo ...Rhodococcus erythropolisBiotechnol. Bioeng.8647-542004-1-----------1--------6-1---11------1---------------------6-1---11--------
668254OlaniranHaloalkane and haloacid dehalo ...Bacillus sp., Burkholderia sp., Corynebacterium sp., Micrococcus sp., Pseudomonas sp.Chemosphere5527-332004-------------5------62-35---------------------------------62-35---------------
654715BosmaSteady-state and pre-steady-st ...Rhodococcus rhodochrousBiochemistry428047-80532003--1----11-----5--1---1-11-1--111--------1-------11-------1--1-11-1--111---------
656190ChaloupkovaModification of acivity and sp ...Sphingomonas paucimobilisJ. Biol. Chem.27852622-526282003--1-17--3-----4--11--10-13-1--31--------1--17----3-------1--10-13-1--31---------
654629BohacHalide-stabilizing residues of ...Rhodococcus erythropolis, Sphingomonas paucimobilis, Xanthobacter autotrophicusBiochemistry4114272-142802002--1-9--1-----3--13----631---1--------1--9----1-------1----631---1---------
654645OakleyExploring the structure and ac ...Sphingomonas paucimobilisBiochemistry414847-48552002-1-1--22-----3---1--3-3-1--21---3---1--1---232----------3-3-1--21---------
654610KmunicekComparative binding energy ana ...Xanthobacter autotrophicusBiochemistry408905-89172001----1--19-----3---1----191----------------1----19------------191--------------
655080DravisHaloalkane hydrolysis with an ...Rhodococcus rhodochrousBiotechnol. Bioeng.75416-4232001-1-----------2---1----1-------------1---------------------1---------------
247098MarekCrystal structure of a haloalk ...Sphingomonas paucimobilisBiochemistry3914082-140862000-1-1-------1-5--------2-------------1--1---------1--------2---------------
247101LauThe importance of reactant pos ...Xanthobacter autotrophicusProc. Natl. Acad. Sci. USA979937-99422000-------------3---1--1-1-----1---------------------------1-1-----1---------
247091NagataConstruction and characterizat ...Sphingomonas paucimobilisProtein Expr. Purif.17299-3041999-11-2--1---2-4--11--1-5-------------11--2----1---2---1--1-5---------------
247095NewmanHaloalkane dehalogenases: stru ...Rhodococcus erythropolisBiochemistry3816105-161141999-1-11--------1--1-----1-------------1--11------------1----1---------------
247096SchindlerHaloalkane dehalogenases: stea ...Rhodococcus erythropolis, Xanthobacter autotrophicusBiochemistry385772-57781999----2-1----1-2---1----10-----------------2--1-----1--------10---------------
247097PikkemaatCrystallographic and kinetic e ...Xanthobacter autotrophicusBiochemistry3812052-120611999---12------1-2--------2----1-----------12--------1--------2----1----------
247099SmatanovaCrystallization and preliminar ...Sphingomonas paucimobilisActa Crystallogr. Sect. D551231-12331999---1-------1-2--------2----------------1---------1--------2---------------
247100DamborskyAnalysis of the reaction mechn ...Rhodococcus rhodochrous, Sphingomonas paucimobilis, Xanthobacter autotrophicusProtein Eng.12989-9981999-333---------5--------3-------------33-3------------------3---------------
247094KrooshofKinetic analysis and X-ray str ...Xanthobacter autotrophicusBiochemistry3715013-150231998---11--7---1-3--11--1-4----------------11----7---1---1--1-4---------------
247090DamborskySome biochemical properties an ...Arthrobacter sp., Corynebacterium sp., Pseudomonas sp., Rhodococcus erythropolis, Xanthobacter autotrophicusBiotechnol. Appl. Biochem.2619-251997-------------7---4--5-7---------------------------------5-7---------------
247092NagataPurification and characterizat ...Sphingomonas paucimobilisAppl. Environ. Microbiol.633707-37101997-11-1-----12-3--1-----81----1-------11--1-------12---1----81----1---------
247089Schanstra-Kinetic characterization and X ...Xanthobacter autotrophicusBiochemistry3513185-131951996---13--4---1-1--11----4----1-----------13----4---1---1----4----1----------
247086KennesReplacement of tryptophan resi ...Escherichia coliEur. J. Biochem.228403-4071995--1-4--------1--11----1--------------1--4------------1----1---------------
247093PriesHistidine 289 is essential for ...Xanthobacter autotrophicusJ. Biol. Chem.27010405-104111995--1-1-11---2-3--11----4--------------1--1--1-1---2---1----4---------------
247085PrinceHaloalkane dehalogenase caught ...Rhodococcus sp., Xanthobacter autotrophicusTrends Biochem. Sci.193-41994------1------2--------2--------------------1--------------2---------------
247082VerschuerenNon-covalent binding of the he ...Xanthobacter autotrophicusFEBS Lett.323267-2701993---1---------2--------1----------------1------------------1---------------
247083VerschuerenRefined X-ray structures of ha ...Xanthobacter autotrophicusJ. Mol. Biol.232856-8721993---1---------3--------1-----1----------1------------------1-----1---------
247084VerschuerenCrystallographic analysis of t ...Xanthobacter autotrophicusNature363693-6981993---1---------2---1----2----------------1------------------2---------------
659633Kasai-Preparation of (S)-2,3-dichlor ...Alcaligenes sp.J. Indust. Microbiol. Biotechnol.997-1011992-1-----------1--------1-1---1-------1---------------------1-1---1---------
247081FrankenCrystal structure of haloalkan ...Xanthobacter autotrophicusEMBO J.101297-13021991---1---------3--------1----------------1------------------1---------------
247088SallisIsolation and characterization ...Rhodococcus erythropolisJ. Gen. Microbiol.136115-1201990------91--1--4--1---1-41----11-------------9-1--1----1--1-41----11--------
247087RozeboomCrystallization of haloalkane ...Xanthobacter autotrophicusJ. Mol. Biol.200611-6121988---1---------3--------1----------------1------------------1---------------
247079YokotaPurification and properties of ...Corynebacterium sp.J. Bacteriol.1694049-40541987-------1--1--4--1---1-2411-3-1-1--------------1--1----1--1-2411-3-1-1-------
247080ScholtzCharacterization of 1-chlorohe ...Arthrobacter sp.J. Bacteriol.1695016-50211987------38--1--3--1---1-7-1---1--------------3-8--1----1--1-7-1---1---------
247078KeuningPurification and characterizat ...Xanthobacter autotrophicusJ. Bacteriol.163635-6391985-1----41--1--3--1----11211-1-1-------1------4-1--1----1---11211-1-1---------