Kar3 interaction with Cik1 alters motor structure and function

Chu, H.M.; Yun, M.; Anderson, D.E.; Sage, H.; Park, H.W.; Endow, S.A.; EMBO J. 24, 3214-3223 (2005)

 show all sequences of 3.6.4.5

Data extracted from this reference:

Activating Compound
Activating Compound Commentary Organism Structure
Cik1 Cik1 and Kar3 stalk regions preferentially associate with one another rather than forming homodimers. Kar3/Cik1 moves on microtubules at 0.002-0.0024 mm/min, 2-5fold faster than Kar3, and destabilizes microtubules at the lagging ends. Structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo Saccharomyces cerevisiae
-
Cloned(Commentary)
Commentary Organism
His6Kar3/Cik1-S tag protein Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
15000
-
 His6Kar3/Cik1-S tag protein, gel filtration Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
-
 ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary Organism
His6Kar3/Cik1-S tag protein Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + H2O
-
 668405 Saccharomyces cerevisiae ADP + phosphate
-
-
-
 ?
Subunits
Subunits Commentary Organism
More Cik1 and Kar3 stalk regions preferentially associate with one another rather than forming homodimers. Kar3/Cik1 moves on microtubules at 0.002-0.0024 mm/min, 2-5fold faster than Kar3, and destabilizes microtubules at the lagging ends. Structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound Commentary Organism Structure
Cik1 Cik1 and Kar3 stalk regions preferentially associate with one another rather than forming homodimers. Kar3/Cik1 moves on microtubules at 0.002-0.0024 mm/min, 2-5fold faster than Kar3, and destabilizes microtubules at the lagging ends. Structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo Saccharomyces cerevisiae
-
Cloned(Commentary) (protein specific)
Commentary Organism
His6Kar3/Cik1-S tag protein Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
15000
-
 His6Kar3/Cik1-S tag protein, gel filtration Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
-
 ?
Purification (Commentary) (protein specific)
Commentary Organism
His6Kar3/Cik1-S tag protein Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + H2O
-
 668405 Saccharomyces cerevisiae ADP + phosphate
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
More Cik1 and Kar3 stalk regions preferentially associate with one another rather than forming homodimers. Kar3/Cik1 moves on microtubules at 0.002-0.0024 mm/min, 2-5fold faster than Kar3, and destabilizes microtubules at the lagging ends. Structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo Saccharomyces cerevisiae

See also following references to EC number 3.6.4.5 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
713213HouA coordinated molecular fishin ...Saccharomyces cerevisiaePhys. Biol.70360032010-------------1---1-----1-----------------------------------1---------11---
700097CaiKinesin-14 family proteins HSE ...Homo sapiens, Xenopus laevisMol. Biol. Cell201348-13592009--2-1-3----2-5--2--1--4--------------2--1--3-----2---2-1--4---------------
700350BraunThe kinesin-14 Klp2 organizes ...Schizosaccharomyces pombeNat. Cell Biol.11724-73020091-1-------12-3--1-----31---------1-1-1----------12---1----31-------1------
712404KocikInteractions between subunits ...Drosophila melanogasterJ. Biol. Chem.28435735-357452009--1-5---1----2--1-----121--91--------1--5-----1------1----121--91----11---
698412FriedmanInhibition of rat testicular n ...Rattus norvegicusJ. Agric. Food Chem.566024-60302008------3-1----3-----1--1--------------------3--1--------1--1---------------
698861FurutaDiffusion and directed movemen ...Schizosaccharomyces pombeJ. Biol. Chem.28336465-3647320081-1----1---1-3--1-----21---1-------1-1-------1---1---1----21---1----------
685072ItoCoiled coil in the stalk regio ...Drosophila melanogasterBiochemistry453315-33242006-------------1----1----1------------------------------1----1--------------
688934HiroseLarge conformational changes i ...Saccharomyces cerevisiaeMol. Cell23913-9232006--11---------1-----------------------1-1----------------------------------
689146EndresA lever-arm rotation drives mo ...Drosophila melanogasterNature439875-8782006---12--------2-------------------------12---------------------------------
667670RobinEssential kinesins: characteri ...Caenorhabditis elegansBiochemistry446526-653620051-1------111-3--------21---1-------1-1---------111--------21---1----------
668333SproulCik1 targets the minus-end kin ...Saccharomyces cerevisiaeCurr. Biol.151420-14272005-------------3--------11----------------------------------11--------------
668405ChuKar3 interaction with Cik1 alt ...Saccharomyces cerevisiaeEMBO J.243214-322320051-1-------11-4--1-----11-----------1-1----------11---1----11--------------
669577GoshimaMechanisms for focusing mitoti ...Drosophila melanogasterJ. Cell Biol.171229-2402005--------1--1-1-----1--2-----------------------1--1-----1--2---------------
656259MackeyMechanistic analysis of the Sa ...Saccharomyces cerevisiaeJ. Biol. Chem.27951354-513612004-------2-----1---1----1----1-----------------2------------1----1----------
657073PreussA plant-specific kinesin binds ...Gossypium hirsutumPlant Physiol.1363945-39552004--1-----1--1-3--------21---------1---1--------1--1--------21-------1------
655282MaddoxThe minus end-directed motor K ...Schizosaccharomyces pombeCurr. Biol.131423-14282003-----------1-1---1----1--------------------------1--------1---------------
655338YunRotation of the stalk/neck and ...Drosophila melanogasterEMBO J.225382-53892003---13--------1---------1---------------13------------------1--------------
656342KhodjakovMinus-end capture of preformed ...Chlorocebus aethiops, Potorous tridactylusJ. Cell Biol.160671-6832003------2------2-----2-----------------------2-----------2------------------
655191MarcusArabidopsis thaliana protein, ...Arabidopsis thalianaCell Motil. Cytoskeleton52144-1502002--1----------1--------2--------------1--------------------2---------------
655330WendtMicroscopic evidence for a min ...Drosophila melanogasterEMBO J.215969-59782002-------------1---1----1-----------------------------------1---------------
656339NodaKIFC3, a microtubule minus end ...Mus musculusJ. Cell Biol.15577-882001----1---1--1-2-----4--1-----------------1-----1--1-----4--1---------------
210575MackeyMoving a microtubule may requi ...Drosophila melanogasterBiochemistry391346-13552000--11---1---1-1--1--1--12-------------1-1-----1---1---1-1--12--------------
210551LaneMicrotubule-based endoplasmic ...Xenopus laevisMol. Biol.101909-19221999--------1-11-1---1-2--1-----------------------1-11-----2--1---------------
210572MatulieneFunction of a minus-end-direct ...Cricetulus griseus, Drosophila melanogaster, Homo sapiens, Mus musculus, Saccharomyces cerevisiaeJ. Cell Sci.1124041-40501999--------2--5-5---5----5-----------------------2--5--------5---------------
210574KozielskiThe crystal structure of the m ...Drosophila melanogasterStructure Fold. Des.71407-14161999---1-------1-1---1----1----------------1---------1--------1---------------
210571SablinDirection determination in the ...Drosophila melanogaster, Rattus norvegicusNature395813-8161998--12-------2-2---2-1--21-------------1-2---------2-----1--21--------------
210568BlockerMolecular requirements for bi- ...Bos taurus, Gallus gallus, Mus musculus, Rattus norvegicusJ. Cell Biol.137113-1291997--------3-24-4---4-5--4-----------------------3-24-----5--4---------------
210569SharpExpression of a minus-end-dire ...Cricetulus griseusJ. Cell Sci.1102373-23801997--1--------1-1---1----1--------------1-----------1--------1---------------
210570HenningsenReversal in the direction of m ...Neurospora crassaNature38993-961997--1-------11-1--11----1--------------1----------11---1----1---------------
210548SongBinding sites on microtubules ...Drosophila melanogaster, Drosophila sp., Saccharomyces cerevisiae, Xenopus laevisBiochemistry3511203-112091996--1---1-1-24-4---3----42-------------1-----1--1-24--------42--------------
210567CrevelWeak and strong states of kine ...Drosophila melanogaster, Drosophila sp., Saccharomyces cerevisiaeJ. Mol. Biol.25766-761996-----------3-4---2----4--------------------------3--------4---------------
210545EndowDeterminants of motor polarity ...Aspergillus sp., Cricetulus griseus, Drosophila sp., Homo sapiens, Saccharomyces sp., Schizosaccharomyces pombeBiophys. J.68271S-274S1995-------------6--------6-----------------------------------6---------------
210546KuriyamaCharacterization of a minus en ...Arabidopsis sp., Aspergillus sp., Cricetulus griseus, Drosophila melanogaster, Homo sapiens, Saccharomyces cerevisiaeJ. Cell Biol.1291049-10591995-----------6-8---6----6--------------------------6--------6---------------
210561LombilloMinus-end-directed motion of k ...Dictyostelium sp., Drosophila melanogaster, Homo sapiens, Tetrahymena sp.Nature373161-1641995--------2--4-4---4----4-----------------------2--4--------4---------------
210562WalkerNcd and kinesin motor domains ...Drosophila melanogaster, Drosophila sp.Proc. Natl. Acad. Sci. USA925960-59641995--------1--2-2--11----3-----------------------1--2---1----3---------------
210563ShimizuComparison of the motile and e ...Bos taurus, Doryteuthis pealeii, Drosophila melanogaster, Drosophila sp., Tetrahymena sp.Biochemistry341575-158219952-1---2-2124-5--14-1-1161----2------2-1-----2--2124---1-1-1161----2---------
210565ThrowerMitotic HeLa cells contain a C ...Dictyostelium sp., Homo sapiens, Xenopus laevisEMBO J.14918-9261995------3-2-23-3--13-1--5--------------------3--2-23---1-1--5---------------
210559EndowYeast Kar3 is a minus-end micr ...Arabidopsis sp., Drosophila melanogaster, Saccharomyces cerevisiaeEMBO J.132708-27131994--1--------3-3--13----3----1---------1-----------3---1----3----1----------
210560MiddletonKAR3-encoded kinesin is a minu ...Drosophila melanogaster, Saccharomyces cerevisiaeProc. Natl. Acad. Sci. USA917212-72161994--1-----2-22-4--12----2--------------1--------2-22---1----2---------------
210557WalkerThe Drosophila claret segregat ...Drosophila melanogasterNature347780-7821990--1---3----1-1---1----1--------------1-----3-----1--------1---------------
210558McDonaldThe kinesin-like ncd protein o ...Drosophila melanogasterCell631159-11651990--1--------1-1--11----1--------------1-----------1---1----1---------------