The cadmium transport sites of CadA, the Cd(2+)-ATPase from Listeria monocytogenes

Wu, C.C.; Gardarin, A.; Martel, A.; Mintz, E.; Guillain, F.; Catty, P.; J. Biol. Chem. 281, 29533-29541 (2006)

Data extracted from this reference:

Engineering

Amino acid exchange	Commentary	Organism
C354A	transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity	Listeria monocytogenes
C356A	transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+	Listeria monocytogenes
D692A	transmembrane segment 8, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity	Listeria monocytogenes
E164A	transmembrane segment 4, reduced ATPase activity	Listeria monocytogenes
M149A	transmembrane segment 3, participation in Cd2+ binding, reduced ATPase activity	Listeria monocytogenes
P355A	transmembrane segment 6, reduced ATPase activity	Listeria monocytogenes

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Listeria monocytogenes	-	-	-

Reaction

Reaction	Commentary	Organism
ATP + H2O + Cd2+/in = ADP + phosphate + Cd2+/out	reaction cycle involves two Cd2+ ions	Listeria monocytogenes
ATP + H2O + Cd2+/in = ADP + phosphate + Cd2+/out	reaction cycle involves two Cd2+ ions	Listeria monocytogenes DP-L1553
ATP + H2O + Cd2+/in = ADP + phosphate + Cd2+/out	reaction cycle involves two Cd2+ ions	Listeria monocytogenes EDGe
ATP + H2O + Cd2+/in = ADP + phosphate + Cd2+/out	reaction cycle involves two Cd2+ ions	Listeria monocytogenes F2365
ATP + H2O + Cd2+/in = ADP + phosphate + Cd2+/out	reaction cycle involves two Cd2+ ions	Listeria monocytogenes Sv1/2a EGD-e

Engineering (protein specific)

Amino acid exchange	Commentary	Organism
C354A	transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity	Listeria monocytogenes
C356A	transmembrane segment 6, participation in Cd2+ binding, inactive in transport of Cd2+	Listeria monocytogenes
D692A	transmembrane segment 8, participation in Cd2+ binding, inactive in transport of Cd2+, no ATPase activity	Listeria monocytogenes
E164A	transmembrane segment 4, reduced ATPase activity	Listeria monocytogenes
M149A	transmembrane segment 3, participation in Cd2+ binding, reduced ATPase activity	Listeria monocytogenes
P355A	transmembrane segment 6, reduced ATPase activity	Listeria monocytogenes

See also following references to EC number 3.6.3.3 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
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