Creatinine and N-methylhydantoin degradation in two newly isolated Clostridium species

Hermann, M.; Knerr, H.J.; Mai, N.; Gro¯, A.; Kaltwasser, H.; Arch. Microbiol. 157, 395-401 (1992)

 show all sequences of 3.5.1.59

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
3
-
 N-carbamoylsarcosine 0.1 M Tris-buffer, 25°C Clostridium sp.
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
N-carbamoylsarcosine + H2O Clostridium sp. inducible enzyme sarcosine + CO2 + NH3
-
 Clostridium sp. ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Clostridium sp.
-
 strains FS23 and FS41
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.096 0.165 crude extract of cells grown on various inducing substrates Clostridium sp.
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
N-carbamoylsarcosine + H2O
-
 209212 Clostridium sp. sarcosine + CO2 + NH3
-
-
-
 ?
N-carbamoylsarcosine + H2O inducible enzyme 209212 Clostridium sp. sarcosine + CO2 + NH3
-
 209212 Clostridium sp. ?
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
3
-
 N-carbamoylsarcosine 0.1 M Tris-buffer, 25°C Clostridium sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
N-carbamoylsarcosine + H2O Clostridium sp. inducible enzyme sarcosine + CO2 + NH3
-
 Clostridium sp. ?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.096 0.165 crude extract of cells grown on various inducing substrates Clostridium sp.
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
N-carbamoylsarcosine + H2O
-
 209212 Clostridium sp. sarcosine + CO2 + NH3
-
-
-
 ?
N-carbamoylsarcosine + H2O inducible enzyme 209212 Clostridium sp. sarcosine + CO2 + NH3
-
 209212 Clostridium sp. ?

See also following references to EC number 3.5.1.59 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
712888LuoCrystal structure and molecula ...Thermoplasma acidophilumJ. Struct. Biol.169304-3112010--11-----1---3--1-----1--------------1-1-------1-----1----1----------11---
209213ZajcCrystallographic and fluoresce ...Arthrobacter sp.J. Mol. Biol.263269-2831996--11--21-----3--1-----21----1--------1-1---2-1-------1----21----1---------
209209Burtscher-Cloning and expression of N-ca ...Arthrobacter sp.Eur. Pat. Appl., Patent EP476670 A21218 pages1992--1----------1--------1--------------1--------------------1---------------
209211RomaoCrystal structure analysis, re ...Arthrobacter sp.J. Mol. Biol.2261111-11301992--11--21--1--3--11--1-111---1-1------1-1---2-1--1----1--1-111---1-1-------
209212HermannCreatinine and N-methylhydanto ...Clostridium sp.Arch. Microbiol.157395-4011992-------1---1-2------1-2----------------------1---1------1-2---------------
209207KimPurification and characterizat ...Pseudomonas putidaJ. Biol. Chem.26111832-118391986---1--71--31-3-11---1-61111-111--------1---7-1--31--11--1-61111-111-------
209210Shimizu-Evaluation of two alternative ...Pseudomonas putida, Pseudomonas sp.Arch. Microbiol.145322-3281986-----------2-2--------4--------------------------2--------4---------------
209208Deeg-Reagent and methods for determ ...Arthrobacter sp., Moraxella sp.Ger. Offen. DE3248145A118 pp1984-------------2--------2-----------------------------------2---------------