Structural comparison of Escherichia coli L-asparaginase in two monoclinic space groups

Sanches, M.; Barbosa, J.A.; de Oliveira, R.T.; Abrahao Neto, J.; Polikarpov, I.; Acta Crystallogr. Sect. D 59, 416-422 (2003)

show all sequences of 3.5.1.1

Data extracted from this reference:

Crystallization (Commentary)
Crystallization Organism
hanging-drop vapour-diffusion method, X-ray structure of the enzyme, crystallized in a new form, space group C2 and refined to 1.95 A resolution, is compared with that of the previously determined crystal for, space group P2(1) Escherichia coli
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Escherichia coli
-
-
-
Crystallization (Commentary) (protein specific)
Crystallization Organism
hanging-drop vapour-diffusion method, X-ray structure of the enzyme, crystallized in a new form, space group C2 and refined to 1.95 A resolution, is compared with that of the previously determined crystal for, space group P2(1) Escherichia coli


See also following references to EC number 3.5.1.1 (sorted by year of publication):
No.1st authorPub
Med
titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
710917KumarAssessment of physical process ...Pectobacterium carotovorumAppl. Biochem. Biotechnol.163327-3372011-1------1-1--2--1---2-211---1-------1---------1-1----1--2-211---1---------
711503NartaImproved production of L-aspar ...Brevibacillus brevisBiores. Technol.1022083-208520113------------1----------1---1------3------------------------1---1---------
714599OffmanRational engineering of L-aspa ...Escherichia coliBlood1171614-16212011----9--------1--------------------------9----------------------------11---
716076AgarwalEffect of chemical and physica ...Serratia marcescensLett. Appl. Microbiol.52307-3132011-1-----------2----------------------1-------------------------------------
710773Yingyan-Studies on the activity of Asp ...Asparagus officinalisActa Hort.856129-1322010-1-----------1-----4----------------1------------------4------------------
711179BansalStructural stability and funct ...Pyrococcus furiosusBiochemistry (Moscow)75375-3812010--1--11---1--3--1------121--11-------1---1-1----1----1-----121--11--------
711648Amena-Production, purification and c ...Streptomyces gulbargensisBraz. J. Microbiol.41173-1782010----------1--1--1---1-1-1-1-111-----------------1----1--1-1-1-1-111-------
711845QiaoStudy on amino amides and enzy ...Escherichia coliElectrophoresis311565-15712010-1-----1-----1--------1-1-----------1--------1------------1-1-------------
711873Ferrara-High-yield extraction of perip ...Saccharomyces cerevisiaeEnzyme Microb. Technol.4771-762010--1-----1----1-------1-1-------------1--------1----------1-1--------------
713442Kumar-Localization and production of ...Cupriavidus necator, Pectobacterium carotovorum, Serratia marcescensProcess Biochem.45223-2292010--------6----5-----924-3-1---1-----------------6--------924-3-1---1---------
713601KavithaOptimization and purification ...Streptomyces tendaeZ. Naturforsch. C65 C528-5312010----------1--1--1--11---1-----------------------1----1-11---1-------------
716000BunpoThe eIF2 kinase GCN2 is essent ...Mus musculusJ. Nutr.1402020-20272010-------------4-------------------------------------------------------11---
716681ScottiCell-cycle inhibition by Helic ...Helicobacter pyloriPLoS ONE5e138922010------1---1--1---------1-------------------1----1----------1---------11---
695693HymavathiEnhancement of L-asparaginase ...Bacillus circulansAppl. Biochem. Biotechnol.159191-1982009-------------2------------------------------------------------------------
695841KumarDevelopment of medium for enha ...Pectobacterium carotovorumAppl. Microbiol. Biotechnol.84477-4862009-------------2--------1-----------------------------------1---------------
696592NathAn isocratic fluorescence HPLC ...Escherichia coliBiomed. Chromatogr.23152-1592009-------------1-------12----------------------------------12---------------
697899KotziaEngineering thermal stability ...Erwinia chrysanthemi, Pectobacterium carotovorumFEBS J.2761750-17612009--2-4--12-----5--2-----5---5----------2--4----12-------2----5---5-----------
698441HendriksenEvaluating the Potential for E ...Aspergillus oryzaeJ. Agric. Food Chem.574168-41762009--1----------4--------1--1---1-------1--------------------1--1---1--------
699399OzaWithania somnifera (Ashwagandh ...Capsicum annuum, Datura inoxia, Pisum sativum, Solanum lycopersicum, Tamarindus indica, Vigna unguiculata, Withania somniferaJ. Integr. Plant Biol.51201-2062009-------1--1--7--1--61-711--11----------------1--1----1-61-711--11---------
699417TeodorHydrogel-magnetic nanoparticle ...Escherichia coliJ. Mater. Sci. Mater. Med.201307-13142009-------------1--------1-----------------------------------1---------------
710712DhavalaStructure of Helicobacter pylo ...Helicobacter pylori J99Acta Crystallogr. Sect. D651253-12612009---1---------1-------------------------1----------------------------------
711172Abakumova-Antitumor activity of L-aspara ...Pectobacterium carotovorum, Yersinia pseudotuberculosisBiochemistry (Moscow) Suppl. Ser. B3198-2012009--1----------2--1---1---2--------1---1---------------1--1---2------1-22---
711174Gladilina-Cloning, expression, and purif ...Helicobacter pylori J99Biochemistry (Moscow) Suppl. Ser. B389-912009--1----------1--1---1---------1--1---1---------------1--1---------11------
712512Mohamed Ali-Purification and characterizat ...Vigna unguiculataJ. Biol. Res.1129-362009------41-21--1--1--111-51212-11-------------4-1-21----1-111-51212-11--------
713569Saleem Basha-Production of extracellular an ...Streptomyces sp.Trop. J. Pharm. Res.8353-3602009-------1-4---1--1--11-1-21--12---------------1-4-----1-11-1-21--12--------
684146KravchenkoThree-dimensional structures o ...Pectobacterium carotovorumActa Crystallogr. Sect. D64248-2562008---1---------1-------------------------1----------------------------------
684147MichalskaCrystal packing of plant-type ...Escherichia coliActa Crystallogr. Sect. D64309-3202008---1---------2-------------------------1----------------------------------
688561BunpoAlanyl-glutamine consumption m ...Escherichia coliJ. Nutr.138338-3432008-1-----------4-----1----------------1------------------1------------------
695354DhavalaExpression, purification and c ...Helicobacter pyloriActa Crystallogr. Sect. F64740-7422008--11------2--1--1--------------------1-1--------2----1--------------------
695591YongL-Asparaginase in the treatmen ...Escherichia coliAnn. Hematol.88647-6522008-1-----------1----------------------1-------------------------------------
696012CappellettiHelicobacter pylori L-asparagi ...Helicobacter pyloriBiochem. Biophys. Res. Commun.3771222-12262008-11----2--2--2--1-----21--122-------11-------2--2----1----21--122---------
697871PapageorgiouStructural and functional insi ...Pectobacterium carotovorumFEBS J.2754306-43162008--11-2-------5--1-----21-------------1-1-2-----------1----21--------------
698748MichalskaThe mechanism of autocatalytic ...Escherichia coliJ. Biol. Chem.28313388-133972008--111-----1--3--1--------------------1-11-------1----1--------------------
699167WeiPurification of Escherichia co ...Escherichia coliJ. Chromatogr. Sci.46556-5592008--1-4--------1--1-----1--------------1--4------------1----1---------------
701277AppelL-Asparaginase and the effect ...Escherichia coliThromb. Haemost.100330-3372008-------------1------------------------------------------------------------
684973KotziaTailoring structure-function p ...Pectobacterium carotovorumBiochem. J.404337-3432007----11-2-----2--------1---2-------------11---2------------1---2-----------
686239VermaL-asparaginase: a promising ch ...Aliivibrio fischeri, Aspergillus niger, Aspergillus tamarii, Aspergillus terreus, Cyberlindnera jadinii, Enterobacter cloacae, Erwinia aroidea, Erwinia sp., Escherichia coli, Lupinus angustifolius, Lupinus arboreus, Mycobacterium phlei, Nocardia asteroides, Pectobacterium carotovorum, Photobacterium leiognathi, Photobacterium phosphoreum, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Rhodosporidium toruloides, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Serratia marcescens, Sphagnum fallax, Staphylococcus sp., Tetrahymena pyriformis, Thermus thermophilus, Vibrio harveyiCrit. Rev. Biotechnol.2745-622007112---224112-30--5--14--53211-6------112-----2-24112---5-15--53211-6---------
686454Li-Enhancing the thermostability ...Escherichia coliEnzyme Microb. Technol.41523-5272007--1-1--2-----1--1----11-2-61---------1--1----2-------1---11-2-61----------
686619FuPEG-asparaginase ...Escherichia coliExpert. Opin. Pharmacother.81977-19842007-1-----------1----------------------1-------------------------------------
687339PrakashamL-asparaginase production by i ...Staphylococcus sp.J. Appl. Microbiol.1021382-13912007-------------2-----1-----------------------------------1------------------
688014SahuStudies on L-asparaginase enzy ...uncultured actinomyceteJ. Environ. Biol.28465-4742007-------------1------------------------------------------------------------
688015SahuPartial purification and anti- ...Actinomyces sp.J. Environ. Biol.28645-6502007-----------1-1--1-----1--------------------------1---1----1---------------
688355YunCrystal structure and alloster ...Escherichia coliJ. Mol. Biol.369794-8112007---12---5----3-------------------------12-----5---------------------------
690010Neelam-Enhanced activity of L-asparag ...Escherichia coli K-12Res. J. Biotechnol.260-632007--1----------1-----------------------1------------------------------------
668046GeckilEffect of Vitreoscilla hemoglo ...Pseudomonas aeruginosaBiotechnol. J.1203-2082006-1--1-1------2----------------------1---1--1------------------------------
668077AppelChanges in hypercoagulability ...Erwinia chrysanthemi, Escherichia coliBlood Coagul. Fibrinolysis17139-1462006-2---------2-2-----2--4-------------2------------2-----2--4---------------
669496ReinertRole of glutamine depletion in ...Escherichia coli, Wolinella succinogenesJ. Biol. Chem.28131222-312332006-2--2------2-7--2--1--2-------------2---2--------2---2-1--2---------------
669561KotziaL-Asparaginase from Erwinia ch ...Erwinia chrysanthemiJ. Biotechnol.127657-6692006-11-11-5---1-3--1-----4---54-----1--12--11---5---1---2----5---54---1------
669885MichalskaCrystal structure of plant asp ...Lupinus luteusJ. Mol. Biol.360105-11620061-11-----1-1-4--11----21-----------1-1-1-------1-1---1----21--------------
670217JianhuaProbing the antigenicity of E. ...Escherichia coliMol. Biotechnol.3357-652006--1-1------1-1--1-----11-------------1--1--------1---1----11--------------
684128MichalskaStructural aspects of L-aspara ...Arabidopsis thaliana, Escherichia coli, Lupinus luteus, Pyrococcus horikoshiiActa Biochim. Pol.53627-6402006---4----11---4---------2---------------4------11-----------2--------------
684515MishraProduction of L-asparaginase, ...Aspergillus nigerAppl. Biochem. Biotechnol.13533-422006-1-----------6--1---1---------------1----------------1--1-----------------
686244Kislitsyn-Three-dimensional structure of ...Pectobacterium carotovorumCrystallogr. Rep.51811-8162006--1----------1-----------------------1------------------------------------
686431Ferrara-Asparaginase production by a r ...Saccharomyces cerevisiaeEnzyme Microb. Technol.391457-14632006--1-----1----1--------1--------------1--------1-----------1---------------
687060KamblePurification of L-asparaginase ...Pectobacterium carotovorumIndian J. Biochem. Biophys.43391-39420061------------1--1-------1---1------1-----------------1------1---1---------
689669BruneauCo-occurrence of both L-aspara ...Arabidopsis thalianaPlanta224668-6792006--1------3---4-----1--31-------------1---------3-------1--31--------------
661391StamsAsparagine synthetase expressi ...Homo sapiensBlood1054223-42252005-1---------2-1--------2-------------1------------2--------2---------------
667061YaoStructure of the type I L-aspa ...Pyrococcus horikoshiiActa Crystallogr. Sect. DD61294-3012005--11----1--1-3--11----11-------------1-1------1--1---1----11--------------
667091WikmanCrystallization and preliminar ...Pectobacterium carotovorumActa Crystallogr. Sect. FF61407-4092005---1---------3---------1---------------1-------------------1--------------
668132BaillargeonL-asparaginase as a marker of ...Homo sapiensCancer1042858-28612005-1---------1-1--------1-------------1------------1--------1---------------
669556KotziaCloning, expression and charac ...Pectobacterium carotovorumJ. Biotechnol.119309-3232005-11----1---1-5--11----1-1---1-------11-------1---1---1----1-1---1---------
669734GaofuAsparaginase display of polype ...Escherichia coliJ. Immunol. Methods2999-192005--1-1---1--1-3--1-----121---1--------1--1-----1--1---1----121---1---------
655078KrasotkinaOne-step purification and kine ...Pectobacterium carotovorumBiotechnol. Appl. Biochem.39215-2212004-11----4-----3--1---1-41---4-1---1--11-------4-------1--1-41---4-1-1------
665436El-BessoumyProduction, isolation, and pur ...Pseudomonas aeruginosaJ. Biochem. Mol. Biol.37387-3932004-1-----21-12-1--1---1-2121--11------1--------21-12---1--1-2121--11--------
667907ZhangImmobilization of L-asparagina ...Escherichia coliBiomaterials253751-37592004-1--1121--11-3-----1--11224-1-------1---11-2-1--11-----1--11224-1---------
668053ZhangChemical modification of L-asp ...Escherichia coliBiotechnol. Lett.26753-7562004-1--1------1-1--------1-1---1-------1---1--------1--------1-1---1---------
670769KhushooExtracellular expression and s ...Escherichia coliProtein Expr. Purif.3829-362004--1-------11-3--1---2-111---1--------1----------11---1--2-111---1---------
654088SanchesStructural comparison of Esche ...Escherichia coliActa Crystallogr. Sect. D59416-4222003---1---------1-------------------------1----------------------------------
654094LubkowskiAtomic resolution structure of ...Erwinia chrysanthemiActa Crystallogr. Sect. D5984-922003---1---------1-------------------------1----------------------------------
654031KozakA comparison between the cryst ...Escherichia coliActa Biochim. Pol.49509-5132002---1---------3-------------------------1----------------------------------
657205Abdel-Fattah-L-asparaginase production by P ...Pseudomonas aeruginosaProcess Biochem.38115-1222002-------------2-----1-----------------------------------1------------------
654123JaskolskiStructures of two highly homol ...Escherichia coliActa Crystallogr. Sect. DD57369-3772001---1---------1-------------------------1----------------------------------
654600AghaiypourStructural basis for the activ ...Erwinia chrysanthemiBiochemistry405655-56642001---1---------3-------------------------1----------------------------------
654816AghaiypourDo bacterial L-asparaginases u ...Erwinia chrysanthemiBiochim. Biophys. Acta1550117-1282001---1---------1-------------------------1----------------------------------
656776PritsaL-Asparaginase of Thermus ther ...Thermus thermophilusMol. Cell. Biochem.21693-1012001------101--1--3--1---1--1----11---1---------10-1--1----1--1--1----11-1------
208964KozakCrystallization and preliminar ...Escherichia coliActa Crystallogr. Sect. D56509-5112000---1---------3--------1----------------1------------------1---------------
657311DerstEngineering the substrate spec ...Escherichia coliProtein Sci.92009-20172000-1--15--32-----3-----2--3----30--------1---15----32---------2--3----30----------
208966IiboshiL-Asparaginase inhibits the ra ...Escherichia coliBiochem. Biophys. Res. Commun.260534-5391999-------------1--------2-----------------------------------2---------------
208958MohapatraProduction and properties of L ...Mucor sp.Cytobios92165-1731997------6111---1--1-----1-1-2-11--1----------61111-----1----1-1-2-11--------
208957Heeschen-Asparagine catabolism in bryop ...Sphagnum cuspidatum, Sphagnum fallax, Sphagnum fimbriatum, Sphagnum imbricatum, Sphagnum magellanicum, Sphagnum papillosum, Sphagnum russowii, Sphagnum squarrosum, Sphagnum subnitens, Sphagnum subsecundumPhysiol. Plant.97402-4101996-------21-1--10--1---1-101----1----------------21-1----1--1-101----1---------
208962MannaPurification, characterization ...Pseudomonas stutzeriCurr. Microbiol.30291-298199571----141-31--2------1-51113-111----71------14-1-31-------1-51113-111-------
208963Newsted-Engineering resistance to tryp ...Escherichia coliEnzyme Microb. Technol.17575-7641995----11--1----1--------1-----------------11----1-----------1---------------
208965ZhangGuinea pig serum L-asparaginas ...Cavia porcellus, Pseudomonas sp.Comp. Biochem. Physiol. B112607-6121995-1-----1-----2-----21-2-------------1--------1---------21-2---------------
666995Lebedeva-Molecular and catalytic proper ...Pseudomonas chlororaphis subsp. aurantiacaVestn. Ross. Akad. Med. Nauk257-611995------42--12-2--------21------1------------4-2--12--------21------1-------
208961TsavdaridisTwo forms of L-asparaginase in ...Tetrahymena thermophilaBiochem. Mol. Biol. Int.3267-7719942-----11122--4--1---2-11----1------2-------1-1122----1--2-11----1---------
208956Sodek-Asperaginase from the testa of ...Lupinus polyphyllusPhytochemistry3451-561993---------12--1--1--12-11-----------------------12----1-12-11--------------
208955LoughL-Asparaginase from developing ...Lupinus arboreusPhytochemistry311519-15271992-------------2--1--23-11-----------------------------1-23-11--------------
208959KyriakidisAntiproliferative activity of ...Tetrahymena pyriformisMol. Cell. Biochem.96137-1421990------1------1--------3--------------------1--------------3---------------
208960RahaPurification and properties of ...Cylindrocarpon obtusisporumBiochem. Int.21987-100019905----1111-411-2-11---1151113-1------5-----1-11-1-411--11--1151113-1---------
208939WristonAsparaginase ...Acinetobacter calcoaceticus, Azotobacter vinelandii, Cavia porcellus, Chlamydomonas sp., Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Fusarium tricinctum, Lupinus angustifolius, Lupinus arboreus, Lupinus polyphyllus, Pectobacterium carotovorum, Pisum sativum, Proteus vulgaris, Pseudomonas geniculata, Saccharomyces cerevisiae, Serratia marcescens, Wolinella succinogenesMethods Enzymol.113608-6181985-------19--21--18--2--11-20-----16----------------19--21----2-11-20-----16---------
208940ChangPurification and properties of ...Lupinus angustifolius, Lupinus arboreusArch. Biochem. Biophys.20849-581981------122--2--4--2--7123--------------------12-2--2----2-7123---------------
208941Lea-The purification and propertie ...Lupinus polyphyllusPhytochemistry17217-22219782-----171-51--1--1--1-271-----------2-------17-1-51----1-1-271--------------
208942GaffarPurification and some biologic ...Azotobacter vinelandiiAppl. Environ. Microbiol.33508-5141977-----21111-1--4--1---111-1-2-1------------2-11-11-1----1--111-1-2-1---------
208943Sakamoto-Partial purification and some ...Cyberlindnera jadiniiAgric. Biol. Chem.411359-13641977------2-1----1--1-----2-1-5-112------------2--1------1----2-1-5-112-------
208944HandschumacherActive site of L-asparaginase: ...Escherichia coliMethods Enzymol.46432-4351977------1------1--------2--------------------1--------------2---------------
208945JonerPurification and properties of ...Acinetobacter calcoaceticusBiochim. Biophys. Acta438287-29519761----141--1--3--1---113---1--------1-----1-4-1--1----1--113---1-----------
208946DistasioPurification and characterizat ...Wolinella succinogenesJ. Biol. Chem.2516929-69331976-------1--1--1-11---1141----1----------------1--1---11--1141----1---------
208947ChibataImmobilized L-aspartic acid ...Proteus vulgarisMethods Enzymol.34B405-4111974-------------1--1---1-1------------------------------1--1-1---------------
208948WhelanPurification and properties of ...Serratia marcescensBiochim. Biophys. Acta365212-2221974----------1--1-11---1-11----1-------------------1---11--1-11----1---------
208949WristonL-Asparaginase: a review ...Acinetobacter calcoaceticus, Acinetobacter glutaminasificans, Aspergillus niger, Aspergillus oryzae, Aspergillus terreus, Azotobacter agilis, Bacillus coagulans, Brucella abortus, Cavia porcellus, Clostridium cadaveris, Cupriavidus necator, Erwinia aroidea, Escherichia coli, Fusarium tricinctum, Gallus gallus, Geobacillus stearothermophilus, Lablab purpureus, Lupinus luteus, Mycobacterium avium, Mycobacterium bovis, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, Neurospora crassa, Pectobacterium carotovorum, Platyrrhini, Proteus vulgaris, Pseudomonas boreopolis, Pseudomonas fluorescens, Pseudomonas sp., Rattus norvegicus, Saccharomyces cerevisiae, Salmonella typhosa, Serratia marcescens, Staphylococcus sp., Streptomyces griseusAdv. Enzymol. Relat. Areas Mol. Biol.39185-2481973-----1318--16--39-----3--631-----------------1-3-18--16------3--631--------------
208950JonerPurification and properties of ...Acinetobacter calcoaceticusBiochim. Biophys. Acta327146-1561973------61-11--1--1---1131--2-1--------------6-1-11----1--1131--2-1---------
208951LiuPurification and properties of ...Erwinia aroideaCan. J. Microbiol.181953-19571972--------1-1--2--1---1-1-11--1-----------------1-1----1--1-1-11--1---------
208952TosaL-Asparaginase from Proteus vu ...Proteus vulgarisBiochemistry11217-2221972---1---3-----1--1---128-11--11---------1-----3-------1--128-11--11--------
208953Wriston-L-Asparaginase ...Bacillus coagulans, Cavia porcellus, Erwinia aroidea, Escherichia coli, Fusarium tricinctum, Pectobacterium carotovorum, Serratia marcescensThe Enzymes, 3rd Ed. (Boyer, P.D., ed.)4101-1211971-5---252--7--7--1-31--26---1-6-------5----2-5-2--7----131--26---1-6---------
208954LawPurification and properties of ...Bacillus coagulansArch. Biochem. Biophys.147744-7521971-----122-31--1--1---112-1-1-11-----------1-2-2-31----1--112-1-1-11--------
665411RobertsNew procedures for purificatio ...Escherichia coliJ. Bacteriol.952117-21231968-1---------1-2--1---121-------------1------------1---1--121---------------