The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall

Zong, Y.; Mazmanian, S.K.; Schneewind, O.; Narayana, S.V.L.; Structure 12, 105-112 (2004)

Data extracted from this reference:

Crystallization (Commentary)

Crystallization	Organism
hanging-drop vapor diffusion method. Crystal structure of SrtB-DELTA-N30 in complex with two active site inhibitors E64 and MTSET, and with the cell wall substrate analog tripleglycine	Staphylococcus aureus

Localization

Localization

Commentary

Organism

GeneOntology No.

Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
IsdC + H2O	Staphylococcus aureus	the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges	?	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Staphylococcus aureus	-	-	-

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
IsdC + H2O	the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges	647690	Staphylococcus aureus	?	-	-	-	?

Crystallization (Commentary) (protein specific)

Crystallization	Organism
hanging-drop vapor diffusion method. Crystal structure of SrtB-DELTA-N30 in complex with two active site inhibitors E64 and MTSET, and with the cell wall substrate analog tripleglycine	Staphylococcus aureus

Localization (protein specific)

Localization

Commentary

Organism

GeneOntology No.

Textmining

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
IsdC + H2O	Staphylococcus aureus	the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges	?	-	-	?

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
IsdC + H2O	the enzyme cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and rethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges	647690	Staphylococcus aureus	?	-	-	-	?

See also following references to EC number 3.4.22.71 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
718136	Spirig		Sortase enzymes in Gram-positi ...	Staphylococcus aureus	Mol. Microbiol.	82	1044-1059	2011	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
718269	Kang		Crystal structure of Spy0129, ...	Streptococcus pyogenes	PLoS ONE	6	e15969	2011	-	-	1	1	1	-	-	-	1	1	-	-	-	4	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	1	-	-	-	-	-	1	1	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
707406	Oh		Therapeutic effect of (Z)-3-(2 ...	Staphylococcus aureus	Biochem. Biophys. Res. Commun.	396	440-444	2010	-	-	-	-	-	-	1	-	-	-	-	-	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
693248	Mariscotti		The Listeria monocytogenes sor ...	Listeria monocytogenes	J. Biol. Chem.	284	6140-6146	2009	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
680796	Maresso		Activation of inhibitors by so ...	Bacillus anthracis	J. Biol. Chem.	282	23129-23139	2007	-	-	-	1	-	-	1	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
680881	Bentley		Engineering the substrate spec ...	Staphylococcus aureus	J. Biol. Chem.	282	6571-6581	2007	-	-	-	-	1	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
663737	Oh		Inhibition of sortase-mediated ...	Staphylococcus aureus	Appl. Microbiol. Biotechnol.	70	102-106	2006	-	1	-	-	-	-	4	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
664280	Kang		Flavonols inhibit sortases and ...	Staphylococcus aureus	Biol. Pharm. Bull.	29	1751-1755	2006	-	-	1	-	-	-	8	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	8	-	-	1	-	-	-	-	8	8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
680473	Maresso		Surface protein IsdC and sorta ...	Bacillus anthracis	J. Bacteriol.	188	8145-8152	2006	-	-	1	-	-	-	-	-	-	-	-	1	-	5	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
665174	Zink		Importance of srtA and srtB fo ...	Bacillus anthracis	Infect. Immun.	73	5222-5228	2005	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
665617	Marraffini		Anchor structure of staphyloco ...	Staphylococcus aureus	J. Biol. Chem.	280	16263-16271	2005	-	-	-	-	-	-	-	-	-	-	-	1	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
666393	Newton		The svpA-srtB locus of Listeri ...	Listeria monocytogenes	Mol. Microbiol.	55	927-940	2005	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
666890	Pucciarelli		Identification of substrates o ...	Listeria monocytogenes	Proteomics	5	4808-4817	2005	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
647690	Zong		The structure of sortase B, a ...	Staphylococcus aureus	Structure	12	105-112	2004	-	-	-	1	-	-	-	-	1	-	-	1	-	4	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
647691	Bierne		Sortase B, a new class of sort ...	Listeria monocytogenes	J. Bacteriol.	186	1972-1982	2004	-	-	-	-	-	-	-	-	-	-	-	2	-	4	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
666932	Zhang		Structures of sortase B from S ...	Bacillus anthracis, Staphylococcus aureus	Structure	12	1147-1156	2004	-	-	-	2	-	-	-	-	-	-	-	-	-	5	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
647386	Jonsson		The role of Staphylococcus aur ...	Staphylococcus aureus	Microbes Infect.	5	775-780	2003	-	-	-	-	-	-	-	-	-	-	-	1	-	5	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-