Human caspase-7 activity and regulation by its N-terminal peptide

Denault, J.B.; Salvesen, G.S.; J. Biol. Chem. 278, 34042-34050 (2003)

 show all sequences of 3.4.22.60

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.0605
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
0.0646
-
 acetyl-DEVD-4-nitroanilide pH 7.2, 37°C Homo sapiens
Localization
Localization Commentary Organism GeneOntology No. Textmining
additional information human caspase-7 is not a nuclear caspase removal of the N-peptide does not allow an active transport or accumulation of human caspase-7 in the nuclei Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
More Homo sapiens caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases ?
-
-
-
More Homo sapiens the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis ?
-
-
-
poly(ADP-ribose) polymerase + H2O Homo sapiens whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry ?
-
-
 ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Homo sapiens
-
 GenBank Acc. No NM 001227
-
Posttranslational Modification
Posttranslational Modification Commentary Organism
proteolytic modification the 12000 Da and the 11000 Da polypeptides are generated by processing of the CMH-1 protein at Asp198-Ser199 and to a lesser extent at Asp206-Ala207; the N-peptide of caspase-7 must be removed, probably be caspase-3, before efficient activation of the zymogen can occur in vivo. The N-peptide serves to physically sequester the caspase-7 zymogen in a cytosolic location that prevents access by upstream activators, caspase-8, caspase-9 and caspase-10 Homo sapiens
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
acetyl-DEVD-4-nitroanilide + H2O
-
 647669 Homo sapiens acetyl-DEVD + 4-nitroaniline
-
-
-
 ?
acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
-
 647669 Homo sapiens acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
-
-
-
 ?
More caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases 647669 Homo sapiens ?
-
-
-
-
More the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis 647669 Homo sapiens ?
-
-
-
-
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nucear entry 647669 Homo sapiens ?
-
-
-
 ?
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry 647669 Homo sapiens ?
-
-
-
 ?
Turnover Number [1/s]
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
6.9
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
10.3
-
 acetyl-DEVD-p-nitroanilide pH 7.2, 37°C Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.0605
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
0.0646
-
 acetyl-DEVD-4-nitroanilide pH 7.2, 37°C Homo sapiens
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
additional information human caspase-7 is not a nuclear caspase removal of the N-peptide does not allow an active transport or accumulation of human caspase-7 in the nuclei Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
More Homo sapiens caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases ?
-
-
-
More Homo sapiens the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis ?
-
-
-
poly(ADP-ribose) polymerase + H2O Homo sapiens whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry ?
-
-
 ?
Posttranslational Modification (protein specific)
Posttranslational Modification Commentary Organism
proteolytic modification the 12000 Da and the 11000 Da polypeptides are generated by processing of the CMH-1 protein at Asp198-Ser199 and to a lesser extent at Asp206-Ala207; the N-peptide of caspase-7 must be removed, probably be caspase-3, before efficient activation of the zymogen can occur in vivo. The N-peptide serves to physically sequester the caspase-7 zymogen in a cytosolic location that prevents access by upstream activators, caspase-8, caspase-9 and caspase-10 Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
acetyl-DEVD-4-nitroanilide + H2O
-
 647669 Homo sapiens acetyl-DEVD + 4-nitroaniline
-
-
-
 ?
acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O
-
 647669 Homo sapiens acetyl-DEVD + 7-amino-4-fluoromethylcoumarin
-
-
-
 ?
More caspase-7 is the most downstream caspase, overexpression does not lead to the activation of other caspases 647669 Homo sapiens ?
-
-
-
-
More the enzyme is activated during Fas- and tumor necrosis factor-induced apoptosis 647669 Homo sapiens ?
-
-
-
-
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nucear entry 647669 Homo sapiens ?
-
-
-
 ?
poly(ADP-ribose) polymerase + H2O whereas caspase-7 can cleave poly(ADP-ribose) polymerase in vivo, a collaborating caspase facilitates access to poly(ADP-ribose) polymerase, possibly by enhancing nuclear entry 647669 Homo sapiens ?
-
-
-
 ?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
6.9
-
 acetyl-DEVD-7-amido-4-fluoromethylcoumarin pH 7.2, 37°C Homo sapiens
10.3
-
 acetyl-DEVD-p-nitroanilide pH 7.2, 37°C Homo sapiens

See also following references to EC number 3.4.22.60 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
717260SoaresThe catalytic subunit of human ...Homo sapiensBiochemistry509046-90552011----3-2------4-----3--1-1---1-----------3--2-----------3--1-1---1---------
717539EdelmannCaspase-8 and caspase-7 sequen ...Homo sapiensEMBO J.30379-39420111---1---1--1-2-1---2--32-----------1----1-----1--1--1--2--32---------22---
710479LeeLarge-scale expression in Esch ...Homo sapiensProtein Expr. Purif.6929-332010--1-2--5-----3--1-----2----5---------1--2----5-------1----2----5--------55
717785MajerciakCaspase-7 cleavage of Kaposi s ...Homo sapiensJ. Biol. Chem.28511297-113072010----1-2-1--1-3-----6--2-1---1-----------1--2--1--1-----6--2-1---1----22---
717796NakatsumiIdentification of functional r ...Homo sapiensJ. Biol. Chem.28525418-254252010--1-1------1-2-----2--21-------------1--1--------1-----2--21--------------
718030MiscioneComputational evidence for the ...Homo sapiensJ. Phys. Chem. B1144637-46452010------1------2---1-------------------------1------------------------------
696749PodichettyFluorinated isatin derivatives ...Homo sapiensBioorg. Med. Chem.172680-26882009------27------1--1-----1-----------27-------2727---------1----1---------------
696881DoraiExpression of anti-apoptosis g ...Cricetulus griseusBiotechnol. Bioeng.103592-608200911--1-1----1-1-----1--2------------11---1--1-----1-----1--2---------------
696945LamkanfiCaspase-7 deficiency protects ...Mus musculusBlood1132742-274520091---1------1-3-----2--1------------1----1--------1-----2--1---------------
697139ChanTumor suppressor effect of fol ...Homo sapiensCarcinogenesis30114-1212009--1--------1-1-----14--1--------------1-----------1-----14--1---------------
697784JenCordycepin induced MA-10 mouse ...Mus musculusEvid. Based. Complement Alternat. Med.20091-1020091-----1----1-1-----2--1------------1-------1-----1-----2--1---------------
699310BressenotAssessment of apoptosis by imm ...Homo sapiensJ. Histochem. Cytochem.57289-3002009--------3--2-3-----41-3-----------------------3--2-----41-3---------------
700596Bondaravaalpha-ENaC is a functional ele ...Homo sapiensPflugers Arch.458675-6872009-----------1-1-----1--1--------------------------1-----1--1---------------
700916AkhterCaspase-7 activation by the Nl ...Mus musculusPLoS Pathog.5e100036120091-1-1------1-6-1---1--1------------1-1--1--------1--1--1--1---------------
707187AgniswamyConformational similarity in t ...Homo sapiensApoptosis141135-11442009--11--2------2--1-----1---------2----1-1---22--------1----1---------------
679154SongDifferential cleavage of Mst1 ...Homo sapiensCell. Signal.20892-9062008-----------1-2-----2--2--------------------------1-----2--2---------------
695998LiFRET evidence that an isoform ...Homo sapiensBiochem. Biophys. Res. Commun.373325-3292008--1-1--1---2-1-1------4--------------1--1----1---2--1-----4---------------
698101HashimotoPossible involvement of caspas ...Homo sapiensGenes Cells13609-6212008----1------1-2-----2--1-----------------1--------1-----2--1---------------
699113FrydrychSerine protease inhibitors N-a ...Homo sapiensJ. Cell. Biochem.1051501-150620081-----2----1-1-----1--2------------1-------2-----1-----1--2---------------
700210JungApoptotic cell death in TrkA-o ...Homo sapiensMol. Cells2612-1720081----------1-2-1---2--1------------1-------------1--1--2--1---------------
700211JangMining of caspase-7 substrates ...Homo sapiensMol. Cells26152-1572008--1---1----14-1-11-----1711---1--------1-----1-----14--11----1711---1---------
700613SiniscalcoInvolvement of subtype 1 metab ...Rattus norvegicusPharmacol. Res.57223-2332008--1---1------2-----3-----------------1-----1-----------3------------------
700938SteghBcl2L12-mediated inhibition of ...Homo sapiensProc. Natl. Acad. Sci. USA10510703-107082008------2----1-3-----7--1--------------------2-----1-----7--1---------------
700944WalshExecutioner caspase-3 and casp ...Homo sapiens, Mus musculusProc. Natl. Acad. Sci. USA10512815-128192008--2-2------2-4-----3--2-----2--------2--2--------2-----3--2-----2---------
701085KarkiEfficient cleavage of Bid and ...Homo sapiensProtein Pept. Lett.151044-104920081-1-1------1-1-11--2--2-1---1------1-1--1--------1--11-2--2-1---1---------
677982JangCaspase-7 mediated cleavage of ...Homo sapiensBiochem. Biophys. Res. Commun.363388-3942007--1---1----1-1--1-----9--------------1-----1-----1---1----9---------------
679782AgniswamyPlasticity of S2-S4 specificit ...Homo sapiensFEBS J.2744752-47652007---1---------2--------1----------------1------------------1---------------
680829YoungProteolytic cleavage of ataxin ...Homo sapiensJ. Biol. Chem.28230150-301602007-1---------1-4-----1--2-------------1------------1-----1--2---------------
682802SchweigreiterPhosphorylation-regulated clea ...Homo sapiensProteomics74457-44672007------1------3--------1--------------------1--------------1---------------
682831Garcia-LozanoCaspase 7 influences susceptib ...Homo sapiensRheumatology461243-12472007-1-----------2----------------------1-------------------------------------
669509TwiddyCaspase-7 is directly activate ...Homo sapiensJ. Biol. Chem.2813876-388820061-1---2-1-2--2--1--1--11-----------1-1-----2--1-2----1-1--11--------------
669888FangStructural and kinetic analysi ...Homo sapiensJ. Mol. Biol.360654-6662006-11----3-----3--1-----3----5--------11-------3-------1----3----5----------
670408HayashiRelationship between SUMO-1 mo ...Homo sapiensNeurosci. Lett.3975-92006211-----2----2-----4---------------211--------2--------4------------------
670996ChenCaspase-3 and -7 mediate apopt ...Homo sapiensVirology347307-3222006-1----1---2--1-----1----------------1------1----2------1------------------
667232ChiuEtoposide (VP-16) sensitizes p ...Homo sapiensApoptosis10643-6502005111---1------2-----2--2------------111-----1-----------2--2---------------
669391ClarkeCleavage of claspin by caspase ...Homo sapiens, Xenopus laevisJ. Biol. Chem.28035337-35345200512----3-2-1--4-----3--4------------12------3--2-1------3--4---------------
670461GoodeUsing peptidic inhibitors to s ...Homo sapiensOrg. Lett.73529-35322005-11---2------1--1-----1-------------11-----2---------1----1---------------
668416YacobiGonadotropins enhance caspase- ...Rattus norvegicusEndocrinology1451943-195120044-----1------1-----2--1------------4-------1-----------2--1---------------
668912OharaCaspase-2 and caspase-7 are in ...Homo sapiensInfect. Immun.72871-879200411----3------4-----3--2------------11------3-----------3--2---------------
669267SironiSTAT1-induced apoptosis is med ...Homo sapiensJ. Biol. Chem.2794066-40742004-1-----------2-----2----------------1------------------2------------------
669946HoudeCaspase-7 expanded function an ...Homo sapiens, Mus musculusJ. Neurosci.249977-99842004-22-----1----6--2--7--3-------------22--------1------2-7--3---------------
647644Kisselevthe caspase-like sites of prot ...Homo sapiensJ. Biol. Chem.27835869-358772003------2------1------------------2----------22-----------------------------
647669DenaultHuman caspase-7 activity and r ...Homo sapiensJ. Biol. Chem.27834042-340502003-------21--3-2-1------6----2-----------------21--3--1-----6----2----------
644012Strausberg R.L.; Feingold E.A.; Grouse L.H.; Derge J.G.; et al.Generation and initial analysi ...Homo sapiens, Mus musculusProc. Natl. Acad. Sci. USA9916899-169032002-------------2-----1-----------------------------------1------------------
647667YaoitaInhibition of nuclear transpor ...Xenopus laevisBiochem. Biophys. Res. Commun.29179-842002--------1----3--------------------------------1---------------------------
647637BaeProteolytic cleavage of epider ...Homo sapiensFEBS Lett.49116-202001------2----1-1--------2--------------------2-----1--------2---------------
647665EthellCaspase 7 can cleave tumor nec ...Homo sapiensBiochim. Biophys. Acta1541231-2382001-------------3-----1--1--------------------------------1--1---------------
647666RiedlStructural basis for the activ ...Homo sapiensProc. Natl. Acad. Sci. USA9814790-147952001---11--------3---------1---------------11------------------1--------------
647422NakajimaStructure, expression, and fun ...Xenopus laevisJ. Biol. Chem.27510484-104912000--1----------1-----2-----------------1-----------------2------------------
647428ChangProteases from cell suicide: f ...Homo sapiensMicrobiol. Mol. Biol. Rev.64821-8462000-------------1--------1-----------------------------------1---------------
647463EleouetThe viral nucleocapsid protein ...Homo sapiensJ. Virol.743975-39832000-----------1-1-1---1--2--------------------------1--1--1--2---------------
647668BehrensdorfThe endothelial monocyte-activ ...Mus musculusFEBS Lett.466143-1472000------2----1-2-----1--4--------------------2-----1-----1--4---------------
647424Garcia-CalvoPurification and catalytic pro ...Homo sapiensCell Death Differ.6362-3691999-------3-----1--------2----21----------------3------------2----21---------
647423Garcia-CalvoInhibition of human caspases b ...Homo sapiensJ. Biol. Chem.27332608-326131998--1---5------1------------------3----1-----53-----------------------------
647664MachleidtCaspase 7-induced cleavage of ...Homo sapiensFEBS Lett.43651-541998--------1--1-2-----2--2-----------------------1--1-----2--2---------------
647429ThornberryA combinatorial approach defin ...Homo sapiensJ. Biol. Chem.27217907-179111997-------------1-1------1-----------------------------1-----1---------------
647460van de CraenCharacterization of seven muri ...Mus musculusFEBS Lett.40361-691997--1--------1-1-----8--1--------------1-----------1-----8--1---------------
647471TalanianSubstrate specificities of cas ...Homo sapiensJ. Biol. Chem.2729677-96821997------18-----1--------8----1----1----------118------------8----1----------
647635MargolinSubstrate and inhibitor specif ...Homo sapiensJ. Biol. Chem.2727223-72281997------2----1-4--------1--------------------2-----1--------1---------------
647659JuanIdentification and mapping of ...Homo sapiens, Mus musculusGenomics4086-931997-------------2-----5-----------------------------------5------------------
647431Fernandes-AlnemriIn vitro activation of CPP32 a ...Homo sapiensProc. Natl. Acad. Sci. USA937464-74691996-------------1-1------------------------------------1---------------------
647657LippkeIdentification and characteriz ...Homo sapiensJ. Biol. Chem.2711825-18281996--1----------2-11--1--21-------------1--------------11-1--21--------------
647663Pai J.T.; Brown M.S.; Goldstein J.L.Purification and cDNA cloning ...Mesocricetus auratusProc. Natl. Acad. Sci. USA935437-54421996--1-----2----1-11--1--2-----11-------1--------2-----11-1--2-----11--------
647673DuanICE-LAP-3, a novel mammalian h ...Homo sapiensJ. Biol. Chem.2711621-16251996--1--------1-3-----1--1--------------1-----------1-----1--1---------------
647658Fernandes-AlnemriMch3, a novel human apoptotic ...Homo sapiensCancer Res.556045-60521995--1---3----1-1-1---1--41--------1----1-----31----1--1--1--41--------------