beta-Aspartyl-N-acetylglucosaminadase from epididymis

Eylar, E.H.; Murakami, M.; Methods Enzymol. 8, 597-600 (1966)

Data extracted from this reference:

General Stability

General Stability	Organism
stable to lyophilization and freezing after butanol extraction	Ovis ammon

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Ovis ammon	-	sheep	-

Purification (Commentary)

Commentary	Organism
zone electrophoresis on starch block	Ovis ammon

Source Tissue

Source Tissue	Commentary	Organism	Textmining
epididymis	-	Ovis ammon	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	630 units/g protein, eluate of starch block after electrophoresis	Ovis ammon

Storage Stability

Storage Stability	Organism
-20°C stable for several months	Ovis ammon

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1-beta-aspartyl-2-acetamido-1,2-dideoxy-D-glucosylamine + H2O	-	171766	Ovis ammon	N-acetylglucosamine + asparagine	-	171766	Ovis ammon	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.7	-	-	Ovis ammon

pH Range

pH Minimum	pH Maximum	Commentary	Organism
6	8	activity near the maximum, completely inactive at pH 4.4	Ovis ammon

General Stability (protein specific)

General Stability	Organism
stable to lyophilization and freezing after butanol extraction	Ovis ammon

Purification (Commentary) (protein specific)

Commentary	Organism
zone electrophoresis on starch block	Ovis ammon

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
epididymis	-	Ovis ammon	-

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	630 units/g protein, eluate of starch block after electrophoresis	Ovis ammon

Storage Stability (protein specific)

Storage Stability	Organism
-20°C stable for several months	Ovis ammon

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1-beta-aspartyl-2-acetamido-1,2-dideoxy-D-glucosylamine + H2O	-	171766	Ovis ammon	N-acetylglucosamine + asparagine	-	171766	Ovis ammon	?

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.7	-	-	Ovis ammon

pH Range (protein specific)

pH Minimum	pH Maximum	Commentary	Organism
6	8	activity near the maximum, completely inactive at pH 4.4	Ovis ammon

See also following references to EC number 3.2.2.11 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
171768	Haeuw	-	The core-specific lysosomal al ...	Homo sapiens, Rattus rattus	Biochem. J.	297	463-466	1994	-	-	-	-	-	-	-	-	1	-	-	-	-	2	-	-	-	-	-	2	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
171767	Kaverzneva	-	_ber Glykosylamidasen aus Limn ...	Lymnaea stagnalis	Liebigs Ann. Chem.	738	130-135	1970	-	-	-	-	-	-	-	-	-	-	1	1	-	1	-	-	-	1	-	1	-	1	2	-	-	-	1	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	1	-	1	2	-	-	-	1	-	1	1	-	-	-	-	-	-	-	-
171766	Eylar	-	beta-Aspartyl-N-acetylglucosam ...	Ovis ammon	Methods Enzymol.	8	597-600	1966	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	1	-	-	1	1	1	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	1	1	1	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-