Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form

Ohtani, N.; Haruki, M.; Muroya, A.; Morikawa, M.; Kanaya, S.; J. Biochem. 127, 895-899 (2000)

 show all sequences of 3.1.26.4

Data extracted from this reference:

Cloned(Commentary)
Commentary Organism
gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag Escherichia coli
Inhibitors
Inhibitors Commentary Organism Structure
KCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli
NaCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.00026
-
 RNA*DNA hybrid pH 8.5, 30°C, recombinant soluble non-tagged enzyme Escherichia coli
-
Metals/Ions
Metals/Ions Commentary Organism Structure
KCl activates at 50 mM, inhibits at 200 mM Escherichia coli
Mg2+ absolutely dependent on for activity, can be substituted by Mn2+ Escherichia coli
Mn2+ absolutely dependent on for activity, can be substituted by Mg2+ Escherichia coli
additional information no activity in absence of Mg2+ or Mn2+, and in presence of 10 mM of Ba2+, Ca2+, Co2+, Zn2+, Cu2+, Fe2+, or Sr2+ Escherichia coli
-
NaCl activates at 50 mM, inhibits at 200 mM Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
22000
-
 recombinant soluble non-tagged enzyme, gel filtration Escherichia coli
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Escherichia coli
-
 type II enzyme, gene rnhB
-
Purification (Commentary)
Commentary Organism
recombinant insoluble enzyme in a urea-denatured form, recombinant soluble intein-tagged enzyme Escherichia coli
Renatured (Commentary)
Commentary Organism
refolding from purified urea-denatured form with low yield Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
 the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme Escherichia coli
0.75
-
 recombinant soluble nontagged enzyme Escherichia coli
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
RNA*DNA hybrid + H2O
-
 655904 Escherichia coli ?
-
-
-
 ?
Subunits
Subunits Commentary Organism
monomer 1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE Escherichia coli
More helical content of the enzyme structure is 23% Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
30
-
 assay at Escherichia coli
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
9.8
-
 above Escherichia coli
pH Range
pH Minimum pH Maximum Commentary Organism
7 9.8 enzyme is inactive below pH 7.0, activity increases with increasing pH Escherichia coli
Cloned(Commentary) (protein specific)
Commentary Organism
gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag Escherichia coli
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
KCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli
NaCl activates by 50% at 50 mM, inhibits by 90% at 200 mM Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.00026
-
 RNA*DNA hybrid pH 8.5, 30°C, recombinant soluble non-tagged enzyme Escherichia coli
-
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
KCl activates at 50 mM, inhibits at 200 mM Escherichia coli
Mg2+ absolutely dependent on for activity, can be substituted by Mn2+ Escherichia coli
Mn2+ absolutely dependent on for activity, can be substituted by Mg2+ Escherichia coli
additional information no activity in absence of Mg2+ or Mn2+, and in presence of 10 mM of Ba2+, Ca2+, Co2+, Zn2+, Cu2+, Fe2+, or Sr2+ Escherichia coli
-
NaCl activates at 50 mM, inhibits at 200 mM Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
22000
-
 recombinant soluble non-tagged enzyme, gel filtration Escherichia coli
Purification (Commentary) (protein specific)
Commentary Organism
recombinant insoluble enzyme in a urea-denatured form, recombinant soluble intein-tagged enzyme Escherichia coli
Renatured (Commentary) (protein specific)
Commentary Organism
refolding from purified urea-denatured form with low yield Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
 the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme Escherichia coli
0.75
-
 recombinant soluble nontagged enzyme Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
RNA*DNA hybrid + H2O
-
 655904 Escherichia coli ?
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
monomer 1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE Escherichia coli
More helical content of the enzyme structure is 23% Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
30
-
 assay at Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
9.8
-
 above Escherichia coli
pH Range (protein specific)
pH Minimum pH Maximum Commentary Organism
7 9.8 enzyme is inactive below pH 7.0, activity increases with increasing pH Escherichia coli

See also following references to EC number 3.1.26.4 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
713673ReilingPurification and crystallizati ...Staphylococcus aureus subsp. aureusActa Crystallogr. Sect. F6779-822011--11-----2-1-3--1-----1--------------1-1-------2-1---1----1----------11---
714247JohnsonStructural basis of the RNase ...Homo sapiensBiochemistry503903-39122011---1---------1--------1----------------1------------------1---------------
714345RuhanenInvolvement of DNA ligase III ...Homo sapiensBiochim. Biophys. Acta18132000-20072011----1---2----3-----1--------------------1-----2--------1-------------11---
715036MiyashitaIdentification of the substrat ...Geobacillus stearothermophilusFEBS Lett.5852313-23172011--117--4-2-1-2--1---12-3-1---1--------1-17----4-2-1---1--12-3-1---1----11---
715580ReijnsThe structure of the human RNa ...Homo sapiensJ. Biol. Chem.28610530-105392011--119--7---1-4--------31---61--------1-19----7---1--------31---61----33-66
715581FigielThe structural and biochemical ...Homo sapiensJ. Biol. Chem.28610540-105502011---130---12-1-4--------2---1-1----------130-----12-1--------2---1-1----11---
716693TakanoStabilization by fusion to the ...Sulfolobus tokodaiiPLoS ONE6e162262011---12--------3------------1------------12---------------------1-----------
704328WatkinsStructural and functional char ...Mycobacterium tuberculosisJ. Bacteriol.1922878-28862010--111--------3--------2--------------1-11-----------------2---------------
707438KitamuraCharacterization of RNase HII ...Pyrococcus furiosusBiochem. J.426337-3442010--113--4-1---4--------21---6---------1-13----4-1----------21---6----------
708119HuA quadruplex-based, label-free ...Escherichia coliChemistry162605-26102010-1-----------1-----1----------------1------------------1------------------
709140ShabanThe structure of the mammalian ...Mus musculusJ. Biol. Chem.2853617-36242010---15--------4---1-----1---------------15------------------1--------------
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714962JongrujaThe N-terminal hybrid binding ...Thermotoga maritimaFEBS J.2774474-44892010--1-2--1-4-1-2------2-411---1--------1--2----1-4-1------2-411---1----11---
715367Le LazStructure-specific nuclease ac ...Pyrococcus abyssiJ. Bacteriol.1923689-36982010--1---11-1-1-3--------1-1--12--------1-----1-1-1-1--------1-1--12----11-11
716001LiuSynthesis of 2,4-propylene-bri ...Homo sapiensJ. Org. Chem.757112-71282010----1-14--2---1----------1---1-----------1--14---2------------1---1---------
716211SuzukiAn upstream open reading frame ...Mus musculusMol. Cell. Biol.305123-51342010--1-----3----1---------1-------------1--------3------------1---------11---
716222RychlikCrystal structures of RNase H2 ...Thermotoga maritimaMol. Cell40658-6702010--115----2-1-2--------4-----21-------1-15------2-1--------4-----21---22---
716995BeilhartzHIV-1 ribonuclease H: structur ...Escherichia coli, Homo sapiensViruses2900-9262010---21----1-2-5--------22---------------21------1-2--------22---------11---
693579FujiDerivatives of 5-nitrofuran-2- ...Homo sapiensJ. Med. Chem.521380-13872009------1------1--------1-----------2-------21--------------1---------------
694046Bigotlmo1273, a novel gene involved ...Listeria monocytogenes EGD-eMicrobiology155891-9022009----1--------8--------------------------1---------------------------------
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708487RohmanDestabilization of psychrotrop ...Shewanella oneidensis MR-1FEBS J.276603-6132009---15--------3------5-1---6------------15---------------5-1---6-----------
709259SchmittThermal and mechanical multist ...Escherichia coliJ. Chem. Phys.1312351012009-------------2----1-----------------------------------1-------------------
709285RostaArtificial reaction coordinate ...Bacillus haloduransJ. Comput. Chem.301634-16412009-------------1---1--------------------------------------------------------
709522ConnellThe folding trajectory of RNas ...Escherichia coliJ. Mol. Biol.391450-4602009----2--------3----1---------------------2-------------1-------------------
709523ConnellA single mutation at residue 2 ...Escherichia coliJ. Mol. Biol.391461-4702009----1--------3--------------------------1---------------------------------
709895FluiterFilling the gap in LNA antisen ...Homo sapiensMol. Biosyst.5838-8432009-------------1-----1--1--------------------------------1--1---------------
710142ChonContributions of the two acces ...Homo sapiensNucleic Acids Res.3796-1102009--1-6----2---7--1-----31----1--------3--6------6-----3----93----3---------
710143KauffmannImprovement of RNA secondary s ...Escherichia coliNucleic Acids Res.37e1212009-1-----------1-----1----------------1------------------1------------------
691677PallanInsights into RNA/DNA hybrid r ...Bacillus haloduransCell Cycle72562-25692008---1-----1-1-4--------1----------------1-------1-1--------1---------------
691767MoriguchiSpermine moiety attached to th ...Escherichia coliChem. Pharm. Bull.561259-12632008-------------1------------------------------------------------------------
692746De VivoPhosphodiester cleavage in rib ...Bacillus haloduransJ. Am. Chem. Soc.13010955-109622008----2----1---3--------1-----------------2------1----------1---------------
693672ZhouThe high-resolution NMR struct ...Thermus thermophilusJ. Mol. Biol.384531-5392008----1-----2--3--1------1--1-------------1-------2----1-----1--1-----------
694458SalonDerivatization of DNAs with se ...Bacillus haloduransNucleic Acids Res.367009-70182008---11--------1-------------------------11---------------------------------
677389LoukachevitchCrystallization and preliminar ...Escherichia coliActa Crystallogr. Sect. F6384-882007--11---------1--------1--------------1-1------------------1---------------
678280HoncharenkoComparison of the RNase H clea ...Escherichia coliBiochemistry465635-56462007-------7-1---1-----1--7-11-61----------------7-1-------1--7-11-61---------
678503PatureauInduction of RNase H activity ...Homo sapiensBioconjugate Chem.18421-43020071--------1-1-1--------3-1---1------1-----------1-1--------3-1---1---------
681431BastockThe pH-dependence of the Esche ...Escherichia coliJ. Mol. Biol.368421-4332007--1----1-1---4--11----1----111-------1-------1-1-----1----1----111--------
681692YarringtonMn2+ suppressor mutations and ...Saccharomyces cerevisiaeJ. Virol.819004-90122007----10-1--1-1-6--------1-1---1-----------10--1---1-1--------1-1---1---------
681805LiangBiochemical characterization a ...Chlamydophila pneumoniaeMicrobiology153787-7932007--1-1----1---5--------3-1-1-11-------1--1------1----------3-1-1-11--------
682020LimaThe positional influence of th ...Homo sapiensMol. Pharmacol.7173-822007------1------1--------2-1---1--------------1--------------2-1---1---------
682021LimaHuman RNase H1 discriminates b ...Homo sapiensMol. Pharmacol.7183-912007--1----1-1-1-1--1-----3-1---1--------1-------1-1-1---1----3-1---1---------
682205HouA method for HLA genotyping us ...Chlamydophila pneumoniaeOligonucleotides17433-4432007---------1---4------1-2-1---1------------------1--------1-2-1---1---------
664755NowotnyStepwise analyses of metal ion ...Bacillus halodurans, Homo sapiensEMBO J.251924-19332006--2211----5-1-2--11----321---1--------2-211------5-1---1----321---1---------
664911ChonIdentification of RNase HII fr ...Escherichia coli, Shewanella sp.FEBS J.2732264-22752006--1--4-3-121--6--1---4-61222-22-------1---4---3-121----1--4-61222-22--------
664916KochiwaStage-specific expression of C ...Caenorhabditis elegansFEBS J.273420-4292006--1----1-2---4--1--14-2--------------1-------1-2-----1-14-2---------------
666070ChonCrystal structure and structur ...Geobacillus stearothermophilusJ. Mol. Biol.356165-1782006--115----2-1-4---1--9-511---2--------1-15------2-1------9-511---2---------
666879LivesayConserved quantitative stabili ...Escherichia coli, Thermus thermophilusProteins62130-1432006-------2---2-2---2----22--2------------------2---2--------22--2-----------
677553TangControlling RNA digestion by R ...Homo sapiensAngew. Chem.453523-3526200611-----1---1-1--------3-1-1-1------11--------1---1--------3-1-1-1---------
663628FangDetermination of ribonuclease ...Escherichia coliAnal. Chem.776528-65342005-------1-----2-----1-11-1--11----------------1---------1-11-1--11---------
666037TsunakaIdentification of single Mn2+ ...Escherichia coliJ. Mol. Biol.3451171-11832005--113----1---3--11-------------------1-13------1-----1--------------------
666288MisraA type II ribonuclease H from ...Leishmania donovani, Leishmania majorMol. Biochem. Parasitol.143135-1452005-22-----3--2-5-22--4--22------------22--------3--2--22-4--22--------------
666490BudihasSelective inhibition of HIV-1 ...Escherichia coli, Homo sapiensNucleic Acids Res.331249-12562005------16------6--------2---------2----------162-------------2---------------
664359ChonGene cloning and biochemical c ...Geobacillus stearothermophilusBiosci. Biotechnol. Biochem.682138-21472004--1---3--41--5--1-----31111-1----1---1-----3---41----1----31111-1--1------
665555LimaStructural Requirements at the ...Homo sapiensJ. Biol. Chem.27936317-363262004--1----------1--11----2-1---1--------1---------------1----2-1---1---------
654694TsunakaDispensability of glutamic aci ...Escherichia coliBiochemistry423366-33742003--1-9-1--3---3---1--6-1-1---1--------1--9--1---3--------6-1-1---1---------
654714LaiMetal ion binding and enzymati ...Methanocaldococcus jannaschiiBiochemistry42785-7912003--1-4--2-3---3--11----3----211-------1--4----2-3-----1----3----211--------
655784MangosEfficient RNase H-directed cle ...Escherichia coli, Homo sapiensJ. Am. Chem. Soc.125654-6612003---------4-2-4--------8-2--22------------------4-2--------8-2--22---------
656122LimaHuman RNase H1 activity is reg ...Homo sapiensJ. Biol. Chem.27814906-1491220032-1-5-1--3-1-1--1---1-3-1---1------2-1--5--1---3-1---1--1-3-1---1---------
655447ten AsbroekThe involvement of human ribon ...Homo sapiensEur. J. Biochem.269583-5922002--1--12-22-1-3--1--61-2-1---1--------1---1-2--22-1---1-61-2-1---1---------
657193RydbergExcision of misincorporated ri ...Escherichia coli, Homo sapiens, Pyrococcus furiosus, Saccharomyces cerevisiaeProc. Natl. Acad. Sci. USA9916654-166592002----1----8---8--2--3--9-4---4-----------1------8-----2-3--9-4---4---------
656012BhagwatBacteriophage T4 RNase H remov ...Enterobacteria phage T4J. Biol. Chem.27628516-285242001-----------2-3--------6-1---1--------------------2--------6-1---1---------
656489ChapadosStructural biochemistry of a t ...Archaeoglobus fulgidusJ. Mol. Biol.307541-5562001--114--5-2-1-3--11----511--41--------1-14----5-2-1---1----511--41---------
657146GromihaFactors influencing the stabil ...Thermus thermophilusPrep. Biochem. Biotechnol.31103-1122001-----1-------2---------1--1--------------1-----------------1--1-----------
657287MuroyaCatalytic center of an archaea ...Thermococcus kodakarensisProtein Sci.10707-7142001--116--8-4---3--11----1-1---1--------1-16----8-4-----1----1-1---1---------
654408KobilFunctional analysis of the dom ...Trypanosoma bruceiBiochem. Biophys. Res. Commun.270336-3422000--1-1-1-12---3--11----11-------------1--1--1--12-----1----11--------------
654569HiranoEnhancement of the enzymatic a ...Thermus thermophilusBiochemistry3913285-132942000--1-7--8-1---5--1---1-1-2--91--------1--7----8-1-----1--1-1-2--91---------
655904OhtaniCharacterization of ribonuclea ...Escherichia coliJ. Biochem.127895-8992000--1---21-51--4--1-1-2-121---11-------1-----2-1-51----11-2-121---11--------
657218HarukiEfficient cleavage of RNA at h ...Escherichia coli, Thermus thermophilusProtein Eng.13881-8862000--2-6--------6---1--2-415-3-2--------2--6---------------2-415-3-2---------
657310GoedkenDivalent metal cofactor bindin ...Escherichia coliProtein Sci.91914-19212000--1111-1-1---3---1-----11---1--------1-111---1-1-----------11---1---------
134402NakaiSequence-specific cleavage of ...Escherichia coliFEBS Lett.33967-721994-1--1--------1--------1-------------1---1-----------------1---------------
134404HuangMetallobiochemistry of the mag ...Escherichia coliEur. J. Biochem.219253-2601994---------2---3--------1------------------------2----------1---------------
134405DaboraEquilibrium unfolding of Esche ...Escherichia coliProtein Sci.31401-14081994----1--------3--1-----1-----------------1------------1----1---------------
134406FrankPurification and characterizat ...Homo sapiensNucleic Acids Res.225247-52541994------62-21--3--1--21-2-----11-------------6-2-21----1-21-2-----11--------
134408CazenaveCharacterization and subcellul ...Xenopus laevisJ. Biol. Chem.26925185-251921994--------3-2--4-----3--11----------------------3-2------3--11--------------
134411CazenaveCharacterization of ribonuclea ...Oryctolagus cuniculus, Triticum aestivumBiochimie75113-1221993------4--3---5-----4--2--------------------4---3-------4--2---------------
134400KanayaA hybrid ribonuclease H. A nov ...Escherichia coliJ. Biol. Chem.2678492-84981992----1--------3--------1-----------------1-----------------1---------------
134403KanayaExpression, purification, and ...Escherichia coli, Thermus thermophilusJ. Biol. Chem.26710184-101921992--1---24-21--5--1-----211-421--------1-----2-4-21----1----211-421---------
134409EderRibonuclease H from K562 human ...Homo sapiensJ. Biol. Chem.2666472-64791991------1--411-4--1--21-51----1--------------1---411---1-21-51----1---------
134080ItayaIsolation and characterization ...Escherichia coliProc. Natl. Acad. Sci. USA878587-85911990--1-------11-3--1-1-113--------------1----------11---11-113---------------
134373RongOn the molecular weight and su ...Bos taurusBiochemistry29383-3891990----------2--2-----1--11------------------------2------1--11--------------
134401YangExpression, purification, and ...Escherichia coliJ. Biol. Chem.26513553-135591990---1---------3--1---1-1----------------1-------------1--1-1---------------
134412VonwirthCalf thymus ribonuclease H IIa ...Bos taurusExperientia46319-3211990---------22--2-----1--21-----------------------22------1--21--------------
134377KanayaOverproduction and preliminary ...Escherichia coliJ. Biol. Chem.26411546-115491989---1---------3--1-----1----------------1-------------1----1---------------
134078WintersbergerThree ribonucleases H and a re ...Saccharomyces cerevisiaeBiochim. Biophys. Acta951322-3291988---------2---2--------2------------------------2----------2---------------
134383KarwanIn addition to RNase H(70) two ...Saccharomyces cerevisiaeJ. Biol. Chem.26314970-149771988---------11--3--11--1-2------------------------11----1--1-2---------------
134381KarwanRibonuclease H(70) from Saccha ...Saccharomyces cerevisiaeProc. Natl. Acad. Sci. USA835919-59231986-------------3--------2-----------------------------------2---------------
134382KarwanYeast ribonuclease H(70) cleav ...Saccharomyces cerevisiaeFEBS Lett.206189-1921986-------------2--------1-----------------------------------1---------------
134068Crouch-Ribonuclease H ...Bos taurus, Escherichia coli, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Xenopus laevisCold Spring Harbor Monogr. Ser.14211-2541982------2-3--2-7-----6--9--------------------2--3--2-----6--9---------------
134372BuesenThe subunit structure of calf ...Bos taurusJ. Biol. Chem.2577106-71081982-------------2-----1--12-------------------------------1--12--------------
134380CheriathundamPurification and properties of ...Saccharomyces cerevisiaeArch. Biochem. Biophys.219110-1201982------1--13--2------1-3---1---2------------1---13-------1-3---1---2-------
134392KitaharaPurification and properties of ...Gallus gallusJ. Biochem.92855-86419821-----7--22--1--1--11-1-----2------1-------7---22----1-11-1-----2---------
134391DirksenSelective inhibition of RNase ...Escherichia coliJ. Biol. Chem.25611569-115731981------1------2--------2--------------------1--------------2---------------
134371BuesenPurification, subunit structur ...Bos taurusJ. Biol. Chem.2559434-944319801-----1-131--4--1--11-11-----------1-------1--131----1-11-11--------------
134390SawaiMultiple forms of ribonuclease ...Rattus norvegicusJ. Biochem.86757-76419791-----2-22---2--1--21-1------------1-------2--22-----1-21-1---------------
134394SawaiTwo ribonucleases H from cultu ...Daucus carotaJ. Biochem.851301-130819792-----1--22--3--1--1--2-----2------2-------1---22----1-1--2-----2---------
134399CathalaIsolation and characterization ...Mus musculusJ. Biol. Chem.2547353-736119791-----82-52--1--1--1--11-----------1-------8-2-52----1-1--11--------------
134370StavrianopoulosSimplified method for purifica ...Bos taurusProc. Natl. Acad. Sci. USA754140-41441978------2--31--2--1--11-1--------------------2---31----1-11-1---------------
134387TashiroRibonuclease H from rat liver. ...Rattus norvegicusJ. Biochem.84385-39319781-----2-321--3--1--2111-----1------1-------2--321----1-2111-----1---------
134388TashiroRibonuclease H from rat liver. ...Rattus norvegicusJ. Biochem.84395-4021978--------3-2--3--1--2--2-----------------------3-2----1-2--2---------------
134389SawaiTwo ribonuclease H activities ...Rattus norvegicusBiochem. Biophys. Res. Commun.84313-3211978------1-211--2--1--2--1-----1--------------1--211----1-2--1-----1---------
134393SawaiRibonuclease H activity in cul ...Daucus carotaBiochim. Biophys. Acta518181-1851978---------1---3-----1--2-----1------------------1-------1--2-----1---------
134076Wyers-Role of DNA-RNA hybrids in euk ...Saccharomyces cerevisiaeEur. J. Biochem.69377-3831976-------------1--1-----1------------------------------1----1---------------
134077Wyers-Role of DNA-RNA hybrids in euk ...Saccharomyces cerevisiaeEur. J. Biochem.69385-39519761--------21--1--1-----11-----------1-----------21----1----11--------------
134369StavrianopoulosRibonuclease H of calf thymus: ...Bos taurusProc. Natl. Acad. Sci. USA731087-10911976------2--3---3-----1--3--------------------2---3-------1--3---------------
134396TashiroMultiple forms of nuclear ribo ...Tetrahymena pyriformisEur. J. Biochem.65123-1301976------1-22---4--1-----1-----1--------------1--22-----1----1-----1---------
134386RoewekampPurification and characteristi ...Rattus norvegicusEur. J. Biochem.43405-4131974------3-221--1--1--1--21----11-------------3--221----1-1--21----11--------
134395CooperMultiple ribonuclease H activi ...Mesocricetus auratusFEBS Lett.4538-431974------3--3---1-----1-11-----6--------------3---3-------1-11-----6---------
134397BanksA ribonuclease H from Ustilago ...Ustilago maydisEur. J. Biochem.47499-50719742-----1--31--2--1---1-1-1---1------2-------1---31----1--1-1-1---1---------
134367HaberkernStudies on a calf thymus ribon ...Bos taurusBiochemistry122389-23951973------61121--1--1--11-2--------------------6-1121----1-11-2---------------
134368StavrianopoulosPurification and properties of ...Bos taurusProc. Natl. Acad. Sci. USA701959-19631973------4--32--1-----11-3--------------------4---32------11-3---------------
134374HenryA hybridase from Escherichia c ...Escherichia coliBiochem. Biophys. Res. Commun.50603-6111973-------------1--1---1-1------------------------------1--1-1---------------
134375MillerRibonuclease H (hybrid) in Esc ...Escherichia coliJ. Biol. Chem.2482621-26241973------1---1--2--1-----1---1----------------1----1----1----1---1-----------
134376BerkowerIsolation and characterization ...Escherichia coliJ. Biol. Chem.2485914-592119731-----1--1---1--1-----3-----1