Biomass production, purification and characterization of chlorophyllase from alga (Phaeodactylum tricornutum)

Kermasha, S.; Khalyfa, A.; Marsot, P.; Alli, I.; Fournier, R.; Biotechnol. Appl. Biochem. 15, 142-159 (1992)

 show all sequences of 3.1.1.14

Data extracted from this reference:

Activating Compound
Activating Compound Commentary Organism Structure
dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
Inhibitors
Inhibitors Commentary Organism Structure
diisopropyl fluorophosphate
-
 Phaeodactylum tricornutum
iodoacetamide
-
 Phaeodactylum tricornutum
NEM
-
 Phaeodactylum tricornutum
Localization
Localization Commentary Organism GeneOntology No. Textmining
thylakoid membrane
-
 Phaeodactylum tricornutum 42651
-
Metals/Ions
Metals/Ions Commentary Organism Structure
Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Phaeodactylum tricornutum
-
-
-
Purification (Commentary)
Commentary Organism
-
 Phaeodactylum tricornutum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
9.79
-
 enzyme isolated at the exponential growth phase Phaeodactylum tricornutum
134.4
-
 enzyme isolated at the stationary growth phase Phaeodactylum tricornutum
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Phaeodactylum tricornutum
pH Range
pH Minimum pH Maximum Commentary Organism
7.5 8.8 pH 7.5: about 35% of maximal activity, pH 8.8: about 65% of maximal activity Phaeodactylum tricornutum
Activating Compound (protein specific)
Activating Compound Commentary Organism Structure
dithiothreitol in combination with Mg2+ dithiothreitol can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
diisopropyl fluorophosphate
-
 Phaeodactylum tricornutum
iodoacetamide
-
 Phaeodactylum tricornutum
NEM
-
 Phaeodactylum tricornutum
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
thylakoid membrane
-
 Phaeodactylum tricornutum 42651
-
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Mg2+ in combination with dithiothreitol Mg2+ can activate chlorophyllase-catalyzed chlorophyll hydrolysis Phaeodactylum tricornutum
Purification (Commentary) (protein specific)
Commentary Organism
-
 Phaeodactylum tricornutum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
9.79
-
 enzyme isolated at the exponential growth phase Phaeodactylum tricornutum
134.4
-
 enzyme isolated at the stationary growth phase Phaeodactylum tricornutum
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Phaeodactylum tricornutum
pH Range (protein specific)
pH Minimum pH Maximum Commentary Organism
7.5 8.8 pH 7.5: about 35% of maximal activity, pH 8.8: about 65% of maximal activity Phaeodactylum tricornutum

See also following references to EC number 3.1.1.14 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
716037BuechertChlorophyllase versus pheophyt ...Brassica oleraceaJ. Plant Physiol.168337-3432011-------------4-----1--1--------------------------------2--2---------2--3--
716496Azoulay-ShemerDual N- and C-terminal process ...Citrus limonPlant Cell Physiol.5270-832011--1-----3----3-1---1--11-------------1--------3-----1--1--11--------------
713678Kaosamphan-Involvement of chlorophyllase ...Brassica oleraceaActa Hort.875111-1182010-----------1-1-----1--1--------------------------1-----1--1---------1111--
715221LeeGenes and biochemical characte ...Brassica oleraceaJ. Agric. Food Chem.588651-86572010--1-6--62----7--1--15-612-162-2------3--12----63------3-35-932-262-2-----66
705875Gupta-Utility of chlorophyllase acti ...Mangifera indicaNational Academy Science Letters3215-172009-------------1------------------------------------------------------------
682459Chen-Transgenic broccoli (Brassica ...Brassica oleraceaPlant Sci.17425-312008--1-1------2-3-----3--3--------------3--3--------3-----7--6---------------
690299Chen-Changes of chlorophyll and ant ...Brassica oleraceaActa Hort.769103-1112008-------------1-----1--1--------------------------------1--1---------------
690844ArkusAn integrated protein chemistr ...Triticum aestivumBiochem. Mol. Biol. Educ.36125-1282008--1-------1--1--1-----1--------------1----------1----1----1---------------
694726Azoulay ShemerCitrus chlorophyllase dynamics ...Citrus limonPlant Physiol.148108-1182008--------2-2--5--1--3--1-----------------------2-2----1-3--1---------------
677636Arriagada-StrodthoffOptimization of chlorophyllase ...Phaeodactylum tricornutumAppl. Biochem. Biotechnol.142263-2752007----1---1--1-3-1----1-2-111-1-----------1-----1--1--1---1-2-111-1---------
679870SchenkThe chlorophyllases AtCLH1 and ...Arabidopsis thalianaFEBS Lett.5815517-55252007--1-1---2--2-4-----1--3-1---1--------2--2-----4--2-----2--4-2---2---------
681243Liao-AtCLH2, a typical but possibly ...Arabidopsis thalianaJ. Integr. Plant Biol.49531-53920071-1-1-1----1-1--1--7--211---1-1----1-1--1--1-----1---1-7--211---1-1-------
682323Harpaz-SaadChlorophyllase is a rate-limit ...Citrus sinensisPlant Cell191007-10222007--1-1---1--1-4--1--1--2-----1--------1--1-----1--1---1-1--2-----1---------
667021Okazawa-Characterization and subcellul ...Ginkgo bilobaZ. Naturforsch. C61111-1172006--1----51----1-1-1-1--5-11--1--------1-------51-----1--1--5-11--1---------
677518ArkusDevelopment of a high-throughp ...Triticum aestivumAnal. Biochem.35393-982006--1---123---2-4--1---1-5-1--22--------1-----12-3---2---1--1-5-1--22---------
678555Kumar-Gender-specific responses of P ...Piper betleBiol. Plant.50705-7082006--------1--1-1-----2--2-1---1-----------------1--1-----2--2-1---1---------
678847YiCharacterization of sol-gel en ...Phaeodactylum tricornutumBiotechnol. Bioeng.95840-84920061---1-111--1-3------1-2-11--1------1----1--1-11--1------1-2-11--1---------
663769ArkusMechanistic analysis of wheat ...Triticum aestivumArch. Biochem. Biophys.438146-1552005-11----4--11-3---1-1114--1-4-----1--11-------4--11-----1114--1-4---1------
665784KarbouneImmobilization and biocatalysi ...Phaeodactylum tricornutumJ. Biotechnol.120273-2832005-1----36-----3---1----2-----1-1-----1------3-6------------2-----1-1-------
666579KariolaChlorophyllase 1, a damage con ...Arabidopsis thalianaPlant Cell17282-2942005-------------6---1--------------------------------------------------------
665148JanavePartial purification of chloro ...Musa cavendishiiIndian J. Biochem. Biophys.41154-1612004-------------1---1--------------------------------------------------------
665817YangHumic substances affect the ac ...Pachira macrocarpaJ. Chem. Ecol.301057-106520043------------5-111-1---------------3----------------11-1------------------
667020Tang-Expression of chlorophyllase i ...Ginkgo bilobaZ. Naturforsch. C59415-4202004--1---1---2--1-1-1-1--1--------------1-----1----2---1--1--1---------------
650503Todorov-Chlorophyllase activity and ch ...Arabidopsis thalianaBiol. Plant.46633-6362003-----------1-1---1-1--1---2----------------------1-----1--1---2-----------
653440RocaInvolvement of chlorophyllase ...Olea europaeaPhysiol. Plant.117459-4662003-----------4-2---1-11-4--------------------------4-----11-4---------------
653472TsuchiyaChlorophyllase as a serine hyd ...Chenopodium albumPlant Cell Physiol.4496-1012003--1-15-5------3---1----1--------------1--15--5--------------1---------------
652956Yi-Encapsulation of chlorophyllas ...Phaeodactylum tricornutumJ. Mol. Catal. B19-20319-3252002-1------1----1-1-1----2-1-----------1---------1-----1-----2-1-------------
653048Hornero-Mendez-Chlorophyll disappearance and ...Capsicum annuumJ. Sci. Food Agric.821564-157020022-----1243--1-1--11-1-35-112-11-----2-------12-43--1---1-1-35-112-11--------
653524BenedettiAltering the expression of the ...Arabidopsis thalianaPlant Physiol.1281255-126320021-1-1-1--1-113--11-4-13-1---1------1-1--1--1---1-11--1-4-13-1---1---------
649092El-SahtEffects of delta-aminolevulini ...Phaseolus vulgarisActa Biol. Hung.5183-902000------1-1--1-2---1-21-1-1---1--------------1--1--1-----21-1-1---1---------
80748ScheumannChlorophyll b reduction during ...Hordeum vulgarePlanta209364-3701999-------------3-----1--1--------------------------------1--1---------------
80752TsuchiyaCloning of chlorophyllase, the ...Chenopodium albumProc. Natl. Acad. Sci. USA9615362-153671999--1--------1-6-----1--1--------------1-----------1-----1--1---------------
80751Gaffar-Biocatalysis of immobilized ch ...Phaeodactylum tricornutumJ. Biotechnol.7445-551998-------4-----1--------3-1---1----------------4------------3-1---1---------
80747Tsuchiya-Purification and characterizat ...Chenopodium albumPlant Cell Physiol.381026-10311997-------4-----1--1--1--21----2----------------4-------1-1--21----2---------
80750Samaha-Biocatalysis of chlorophyllase ...Phaeodactylum tricornutumJ. Biotechnol.55181-19119973-----11-1---1--1-----1------------3-------1-1-1-----1----1---------------
80753Khalyfa-Purification and characterizat ...Phaeodactylum tricornutumAppl. Biochem. Biotechnol.5311-2719952-----2--1---1--1---1-211---1------2-------2---1-----1--1-211---1---------
80746Nishiyama-Purification and substrate spe ...Chlorella regularisChem. Lett.169-721994----------1--1--1---1-21------------------------1----1--1-21--------------
80754Khalyfa-Purification and characterizat ...Phaeodactylum tricornutumBiosci. Biotechnol. Biochem.57433-4371993------11-----1--1---1-2-----2--------------1-1-------1--1-2-----2---------
80730FiedorThe stereospecific interaction ...Melia azedarach, Tetragonia tetragonioidesJ. Biol. Chem.26722043-220471992--------2----3--------6-----------------------2-----------6---------------
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80740Amir-Shapira-Autolysis of chlorophyll in aq ...Citrus sinensis, Petroselinum crispumPlant Sci.43201-2061986--------2--2-2-----2--2-----------------------2--2-----2--2---------------
80728LambersInactivation of chlorophyllase ...Phaeodactylum tricornutumBiochim. Biophys. Acta831225-2351985------2------2--------1--------------------2--------------1---------------
80729Lambers-Molecular mass estimation of c ...Phaeodactylum tricornutumBiochim. Biophys. Acta831213-2241985--------1-1--1-11-----------------------------1-1---11--------------------
80741Lambers-Reconstitution of chlorophylla ...Phaeodactylum tricornutumBiochim. Biophys. Acta7861-81984-------------1------------1-----------------------------------1-----------
80742Terpstra-Interactions between chlorophy ...Phaeodactylum tricornutumBiochim. Biophys. Acta74623-3119832-----1--1---1---------------------2-------1---1--------------------------
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80732Shimokawa-Hydrophobic chromatographic pu ...Citrus unshiuBiochemistry21543-5451982-------2--1--1--1--11-111-1-11---------------2--1----1-11-111-1-11--------
80744Terpstra-Studies on chlorophyllase. The ...Phaeodactylum tricornutumBiochim. Biophys. Acta681233-24119822-----1--1---1-1-------------------2-------1---1----1---------------------
80733Shimokawa-Purification of ethylene-enhan ...Citrus unshiuAgric. Biol. Chem.452357-23591981----------1--1--1--11-11----11------------------1----1-11-11----11--------
80734Terpstra-Identification of chlorophylla ...Phaeodactylum tricornutumFEBS Lett.126231-2351981-------------1-1------------------------------------1---------------------
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80745TerpstraInfluence of lecithin liposome ...Phaeodactylum tricornutumBiochim. Biophys. Acta60036-4719801----11--1---3--------1------------1-----1-1---1----------1---------------
80736Moll-The activity of Triton X-100 s ...Phaseolus vulgarisPlanta14075-801978------2-2----1--1--12-41-------------------2--2------1-12-41--------------
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80738KleinActivity and partial purificat ...Secale cerealeJ. Biol. Chem.2362544-25471961------21-----1--1--1--5-----1--------------2-1-------1-1--5-----1---------