Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coli

Fox, D.K.; Roseman, S.; J. Biol. Chem. 261, 13487-13497 (1986)

 show all sequences of 2.7.2.1

Data extracted from this reference:

General Stability
General Stability Organism
destroyed by repeated freezing and thawing Salmonella enterica subsp. enterica serovar Typhimurium
destroyed by repeated freezing and thawing Escherichia coli
Inhibitors
Inhibitors Commentary Organism Structure
Li+
-
 Escherichia coli
Li+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Na+
-
 Escherichia coli
Na+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Propionic acid competitive inhibitor with respect to acetate Escherichia coli
Propionic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.07
-
 ATP pH 7.9, 37°C Escherichia coli
0.07
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.16
-
 acetyl phosphate pH 7.9, 37°C Escherichia coli
0.16
-
 acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.5
-
 ADP pH 7.9, 37°C Escherichia coli
0.5
-
 ADP pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.78
-
 ITP pH 7.9, 37°C Escherichia coli
0.94
-
 ATP pH 7.9, 37°C Escherichia coli
1.1
-
 GTP pH 7.9, 37°C Escherichia coli
7
-
 acetate pH 7.9, 37°C Escherichia coli
7
-
 acetate pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
68000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
70000
-
 gel filtration Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + acetate Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium r
ATP + acetate Escherichia coli
-
 ADP + acetyl phosphate
-
 Escherichia coli r
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Escherichia coli
-
 K235, 1100 thi-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT2, wild-type
-
Purification (Commentary)
Commentary Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium
partial Escherichia coli
Storage Stability
Storage Stability Organism
-20°C, relatively stable to storage when frozen, remains active for at least 1 year Escherichia coli
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + acetate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium r
ATP + acetate
-
 642182 Escherichia coli ADP + acetyl phosphate
-
 642182 Escherichia coli r
GTP + acetate
-
 642182 Escherichia coli GDP + acetyl phosphate
-
 642182 Escherichia coli r
ITP + acetate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium IDP + acetyl phosphate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium r
ITP + acetate
-
 642182 Escherichia coli IDP + acetyl phosphate
-
 642182 Escherichia coli r
More neither propionic acid nor butyric acid are active as substrates 642182 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-
-
-
More neither propionic acid nor butyric acid are active as substrates 642182 Escherichia coli ?
-
-
-
-
Subunits
Subunits Commentary Organism
dimer 2 * 40000, SDS-PAGE Escherichia coli
dimer 2 * 41200, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.5
-
-
 Escherichia coli
7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
General Stability Organism
destroyed by repeated freezing and thawing Salmonella enterica subsp. enterica serovar Typhimurium
destroyed by repeated freezing and thawing Escherichia coli
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
Li+
-
 Escherichia coli
Li+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Na+
-
 Escherichia coli
Na+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
Propionic acid competitive inhibitor with respect to acetate Escherichia coli
Propionic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.07
-
 ATP pH 7.9, 37°C Escherichia coli
0.07
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.16
-
 acetyl phosphate pH 7.9, 37°C Escherichia coli
0.16
-
 acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
0.5
-
 ADP pH 7.9, 37°C Escherichia coli
0.5
-
 ADP pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.78
-
 ITP pH 7.9, 37°C Escherichia coli
0.94
-
 ATP pH 7.9, 37°C Escherichia coli
1.1
-
 GTP pH 7.9, 37°C Escherichia coli
7
-
 acetate pH 7.9, 37°C Escherichia coli
7
-
 acetate pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
68000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
70000
-
 gel filtration Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + acetate Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium r
ATP + acetate Escherichia coli
-
 ADP + acetyl phosphate
-
 Escherichia coli r
Purification (Commentary) (protein specific)
Commentary Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium
partial Escherichia coli
Storage Stability (protein specific)
Storage Stability Organism
-20°C, relatively stable to storage when frozen, remains active for at least 1 year Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + acetate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium r
ATP + acetate
-
 642182 Escherichia coli ADP + acetyl phosphate
-
 642182 Escherichia coli r
GTP + acetate
-
 642182 Escherichia coli GDP + acetyl phosphate
-
 642182 Escherichia coli r
ITP + acetate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium IDP + acetyl phosphate
-
 642182 Salmonella enterica subsp. enterica serovar Typhimurium r
ITP + acetate
-
 642182 Escherichia coli IDP + acetyl phosphate
-
 642182 Escherichia coli r
More neither propionic acid nor butyric acid are active as substrates 642182 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
-
-
-
More neither propionic acid nor butyric acid are active as substrates 642182 Escherichia coli ?
-
-
-
-
Subunits (protein specific)
Subunits Commentary Organism
dimer 2 * 40000, SDS-PAGE Escherichia coli
dimer 2 * 41200, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.5
-
-
 Escherichia coli
7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

See also following references to EC number 2.7.2.1 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
691204MukhopadhyayA continuous assay of acetate ...Thermotoga maritimaBioorg. Chem.3665-692008------11---1-3--1---1-3-1---11-------------1-1---1---1--1-3-1---11--------
673209RenCloning, expression, and chara ...Ethanoligenens harbinenseCurr. Microbiol.55167-1722007--1-------1--5--1-1-1-1--------1-1---11---------1----11-1-1--------1------
673750BaldockRoseovarius sp. strain 217: ae ...Roseovarius sp.FEMS Microbiol. Lett.271202-2062007-------------3--------1--------1------1-------------------1---------------
690888GorrellInvestigation of the Methanosa ...Methanosarcina thermophilaBiochemistry4614170-141762007--1-5--22-1-1-2--11--1-2----12---1-----11-5----22-1-1---1--1-2----12----------
693619HalbedelTranscription in Mycoplasma pn ...Mycoplasma pneumoniaeJ. Mol. Biol.371596-6072007--1--------1-2--1---1-2--------------1-----------1---1--1-2---------------
672708SuwannakhamConstruction and characterizat ...Propionibacterium acidipropioniciBiotechnol. Bioeng.94383-3952006-------------3--------1--------1------1-------------------1---------------
677237Ingram-SmithAcetate kinase: not just a bac ...Ajellomyces capsulatus, Aspergillus fumigatus, Bacillus subtilis, Borrelia burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Emericella nidulans, Entamoeba histolytica, Enterococcus faecalis, Gibberella zeae, Helicobacter pylori, Hypocrea jecorina, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Phaeosphaeria nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Uncinocarpus reesii, Ustilago maydisTrends Microbiol.14249-2532006-------------40--------37--------37------37-------------------37---------------
659065Ingram-SmithCharacterization of the acetat ...Methanosarcina thermophilaJ. Bacteriol.1872386-23942005---15--27-----4---2----4----21-----------15----27------------4----21----------
659446GorrellStructural and kinetic analyse ...Methanosarcina thermophilaJ. Biol. Chem.28010731-107422005---15-132-----4--------2----13----1------15--1132------------2----13----------
659856Iyer-Acetate kinase from Methanosar ...Methanosarcina thermophilaMethods Biotechnol.17239-2462005-1--1--4-----1--1-----9----2--------1---1----4-------1----9----2----------
659056HasonaPyruvate formate lyase and ace ...Escherichia coliJ. Bacteriol.1867593-76002004----1--------4--------------------------1---------------------------------
642198ItoHighly sensitive simultaneous ...Geobacillus stearothermophilusAnal. Sci.19105-1092003-----------1-2--------1--------------------------1--------1---------------
642195KnorrCloning, expression, and chara ...Lactobacillus sanfranciscensisMicrobiol. Res.156267-2772001--1----4-221-4--1---1-2-111-1--------1-------4-221---1--1-2-111-1---------
642196MilesSite-directed mutational analy ...Methanosarcina thermophilaJ. Biol. Chem.27645059-450642001--1-11--28-3-1-4------9-1--------------1--11----28-3-1------9-1---------------
642197YuPurification and characterizat ...Desulfovibrio vulgarisJ. Biochem.129411-4212001------11-111-4--1---2111----11-------------1-1-111---1--2111----11--------
642193Ingram-SmithThe role of histidines in the ...Methanosarcina thermophilaJ. Biol. Chem.27533765-337702000--1-9-129-----4--1--------------------1--9--1-29-------1--------------------
642194Singh-WissmannIdentification of essential ar ...Methanosarcina thermophilaBiochemistry393671-36772000--1-7-119---1-5--1-----1--------------1--7--1-19---1---1----1---------------
487537BockPurification and characterizat ...Thermotoga maritimaJ. Bacteriol.1811861-18671999-----1-4-611-51-1----171111-11-----------1---4-611-1-1---171111-11--------
642190Lin-Purification and characterizat ...Clostridium thermocellumTetrahedron5415915-159251998-1-----1-211-1--1---1-11111-111-----1--------1-211---1--1-11111-111-------
642191Singh-WissmannIdentification of essential gl ...Methanosarcina thermophilaJ. Bacteriol.1801129-11341998--1-3-221---1-5------1-11-------------1--3--2-21---1------1-11--------------
642188BussCrystallization of acetate kin ...Methanosarcina thermophilaProtein Sci.62659-26621997---1---------3-------------------------1----------------------------------
642189Winzer-Acetate kinase from Clostridiu ...Clostridium acetobutylicumMicrobiology1433279-32861997--1---24-421-1--1---1-71-------------1-----2-4-421---1--1-71--------------
642187Diez-GonzalezThe acetate kinase of Clostrid ...Clostridium acetobutylicumArch. Microbiol.166418-4201996------13-21--1--1---1-21---41--------------1-3-21----1--1-21---41---------
642184Aceti-Purification and characterizat ...Methanosarcina thermophilaJ. Biol. Chem.24915444-154481988-----2-21411-11-1---1181-11-11---1-------2---21411-1-1--1181-11-11-1------
642185KimInactivation of Acinetobacter ...Acinetobacter calcoaceticusBiochim. Biophys. Acta956103-1091988-----12----1-3--1-----1------------------1-2-----1---1----1---------------
642186Mannens-Purification and immobilizatio ...Desulfovibrio vulgarisBiotechnol. Lett.10563-5681988-----------1-1--1---1-1--------------------------1---1--1-1---------------
642182FoxIsolation and characterization ...Escherichia coli, Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.26113487-134971986-----2611--22-3--2----172----2------------2-6-11--22---2---172----2---------
642183VigenschowPurification and properties of ...Rhodopseudomonas palustrisBiol. Chem. Hoppe-Seyler367951-9561986-------11-231-2--1---1281--1-1----1-----------11-231---1--1281--1-1--1------
642181Ichikawa-Purification and properties of ...Propionibacterium freudenreichiiJ. Ferment. Technol.63143-1491985-------6-611-1--1---1-511-1-211--------------6-611---1--1-511-1-211-------
642179HarwoodProperties of acetate kinase i ...Spirochaeta sp.J. Bacteriol.152246-2541982-------6-621-1--------7----------------------6-621--------7---------------
642180Suzuki-Acetate kinase from Bacillus s ...Geobacillus stearothermophilusMethods Enzymol.90179-18519823--1-----311-1--1---1-51--1-1-1----3---1-------311---1--1-51--1-1-1-------
642178Nishimura-Acetate kinase from Veilonella ...Veillonella parvulaMethods Enzymol.71311-31619811--------311-2--1---1151-----------1-----------311---1--1151--------------
642177WongInactivation of Escherichia co ...Escherichia coliBiochim. Biophys. Acta615121-1311980-----13----1-2--------1------------------1-3-----1--------1---------------
642174GriffithAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.254442-44619791-----5---11-2--1---1-11----1------1-------5----11---1--1-11----1---------
642175GriffithAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.2546698-670219791-----24---1-3--------1------1-----1-------2-4---1--------1------1--------
642176KahanePurification and properties of ...Acholeplasma laidlawiiJ. Bacteriol.137764-7721979-----166-141-3--1---1161---5-------------1-6-6-141---1--1161---5----------
642172NakajimaPurification and properties of ...Geobacillus stearothermophilusJ. Biochem.84193-2031978---1-12--621-31-1---1-61--1-111--------1-1-2---621-1-1--1-61--1-111-------
642173YoshimuraPurification and characterizat ...Veillonella parvulaArch. Biochem. Biophys.189424-43219781-----94-511-2--1---1251--1-1-1----1-------9-4-511---1--1251--1-1-1-------
642171BowmanAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.2513117-312119761------10--11-2------1-21-----------1---------10--11------1-21--------------
642168JansonThe inhibition of acetate, pyr ...Escherichia coliJ. Biol. Chem.2492567-25711974------2--1---1-----------------------------2---1--------------------------
642169JansonThe specificity of chromium nu ...Escherichia coliJ. Biol. Chem.2492572-25741974------1--1---1-----------------------------1---1--------------------------
642170SchauppPurification and properties of ...Moorella thermoaceticaArch. Microbiol.100121-1291974-------1--11-2--1-----1-1--------------------1--11---1----1-1-------------
642167AnthonyExchange reactions catalyzed b ...Escherichia coliJ. Biol. Chem.2466129-61351971---------1-1-1--1----11-----111----------------1-1---1---11-----111-------
642166Rose-Acetate kinase ...Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenesThe Enzymes, 2nd. Ed. (Boyer, P.D., Lardy, H., Myrbaeck, K., eds.)6115-1181962-----134-1-3-3--2---1-7-----1-1----------1-3-4-1-3---2--1-7-----1-1-------