Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete

Harwood, C.S.; Canale-Parola, E.; J. Bacteriol. 152, 246-254 (1982)

 show all sequences of 2.7.2.1

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
3.7
-
 ATP pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
3.7
-
 ATP pH 7.5, 30°C, isoenzyme II Spirochaeta sp. MA-2
3.8
-
 ATP pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
3.8
-
 ATP pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
11.7
-
 acetate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
11.7
-
 acetate pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
38.5
-
 acetate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
38.5
-
 acetate pH 7.5, 30°C, isoenzyme II Spirochaeta sp. MA-2
40
-
 Propionate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
40
-
 Propionate pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
133
-
 Propionate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
Metals/Ions
Metals/Ions Commentary Organism Structure
Ca2+ isoenzyme I Spirochaeta sp.
Co2+
-
 Spirochaeta sp.
Co2+
-
 Spirochaeta sp. MA-2
Cu2+
-
 Spirochaeta sp.
Cu2+
-
 Spirochaeta sp. MA-2
Mg2+
-
 Spirochaeta sp.
Mg2+
-
 Spirochaeta sp. MA-2
Mn2+
-
 Spirochaeta sp.
Mn2+
-
 Spirochaeta sp. MA-2
Zn2+
-
 Spirochaeta sp.
Zn2+
-
 Spirochaeta sp. MA-2
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
110000
-
 sucrose density gradient centrifugation, isoenzyme I and II Spirochaeta sp. MA-2
110000
-
 sucrose density gradient centrifugation, isoenzyme I and II Spirochaeta sp.
125000
-
 gel filtration, isoenzyme I and II Spirochaeta sp.
125000
-
 gel filtration, isoenzyme I and II Spirochaeta sp. MA-2
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + acetate Spirochaeta sp.
-
 ADP + acetyl phosphate
-
 Spirochaeta sp. r
ATP + acetate Spirochaeta sp. MA-2
-
 ADP + acetyl phosphate
-
 Spirochaeta sp. MA-2 r
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Spirochaeta sp.
-
-
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + acetate
-
 642179 Spirochaeta sp. ADP + acetyl phosphate
-
 642179 Spirochaeta sp. r
ATP + acetate
-
 642179 Spirochaeta sp. MA-2 ADP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
ATP + propionate
-
 642179 Spirochaeta sp. ADP + propionyl phosphate
-
 642179 Spirochaeta sp. r
CTP + acetate
-
 642179 Spirochaeta sp. CDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
CTP + acetate
-
 642179 Spirochaeta sp. MA-2 CDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
GTP + acetate
-
 642179 Spirochaeta sp. GDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
GTP + acetate
-
 642179 Spirochaeta sp. MA-2 GDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
ITP + acetate
-
 642179 Spirochaeta sp. IDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
ITP + acetate
-
 642179 Spirochaeta sp. MA-2 IDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
More isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates 642179 Spirochaeta sp. ?
-
-
-
-
More isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates 642179 Spirochaeta sp. MA-2 ?
-
-
-
-
UTP + acetate
-
 642179 Spirochaeta sp. UDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
3.7
-
 ATP pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
3.7
-
 ATP pH 7.5, 30°C, isoenzyme II Spirochaeta sp. MA-2
3.8
-
 ATP pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
3.8
-
 ATP pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
11.7
-
 acetate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
11.7
-
 acetate pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
38.5
-
 acetate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
38.5
-
 acetate pH 7.5, 30°C, isoenzyme II Spirochaeta sp. MA-2
40
-
 Propionate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
40
-
 Propionate pH 7.5, 30°C, isoenzyme I Spirochaeta sp. MA-2
133
-
 Propionate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Ca2+ isoenzyme I Spirochaeta sp.
Co2+
-
 Spirochaeta sp.
Co2+
-
 Spirochaeta sp. MA-2
Cu2+
-
 Spirochaeta sp.
Cu2+
-
 Spirochaeta sp. MA-2
Mg2+
-
 Spirochaeta sp.
Mg2+
-
 Spirochaeta sp. MA-2
Mn2+
-
 Spirochaeta sp.
Mn2+
-
 Spirochaeta sp. MA-2
Zn2+
-
 Spirochaeta sp.
Zn2+
-
 Spirochaeta sp. MA-2
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
110000
-
 sucrose density gradient centrifugation, isoenzyme I and II Spirochaeta sp.
110000
-
 sucrose density gradient centrifugation, isoenzyme I and II Spirochaeta sp. MA-2
125000
-
 gel filtration, isoenzyme I and II Spirochaeta sp.
125000
-
 gel filtration, isoenzyme I and II Spirochaeta sp. MA-2
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + acetate Spirochaeta sp.
-
 ADP + acetyl phosphate
-
 Spirochaeta sp. r
ATP + acetate Spirochaeta sp. MA-2
-
 ADP + acetyl phosphate
-
 Spirochaeta sp. MA-2 r
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + acetate
-
 642179 Spirochaeta sp. ADP + acetyl phosphate
-
 642179 Spirochaeta sp. r
ATP + acetate
-
 642179 Spirochaeta sp. MA-2 ADP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
ATP + propionate
-
 642179 Spirochaeta sp. ADP + propionyl phosphate
-
 642179 Spirochaeta sp. r
CTP + acetate
-
 642179 Spirochaeta sp. CDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
CTP + acetate
-
 642179 Spirochaeta sp. MA-2 CDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
GTP + acetate
-
 642179 Spirochaeta sp. GDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
GTP + acetate
-
 642179 Spirochaeta sp. MA-2 GDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
ITP + acetate
-
 642179 Spirochaeta sp. IDP + acetyl phosphate
-
 642179 Spirochaeta sp. r
ITP + acetate
-
 642179 Spirochaeta sp. MA-2 IDP + acetyl phosphate
-
 642179 Spirochaeta sp. MA-2 r
More isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates 642179 Spirochaeta sp. ?
-
-
-
-
More isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates 642179 Spirochaeta sp. MA-2 ?
-
-
-
-
UTP + acetate
-
 642179 Spirochaeta sp. UDP + acetyl phosphate
-
 642179 Spirochaeta sp. r

See also following references to EC number 2.7.2.1 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
691204MukhopadhyayA continuous assay of acetate ...Thermotoga maritimaBioorg. Chem.3665-692008------11---1-3--1---1-3-1---11-------------1-1---1---1--1-3-1---11--------
673209RenCloning, expression, and chara ...Ethanoligenens harbinenseCurr. Microbiol.55167-1722007--1-------1--5--1-1-1-1--------1-1---11---------1----11-1-1--------1------
673750BaldockRoseovarius sp. strain 217: ae ...Roseovarius sp., Roseovarius sp. 217FEMS Microbiol. Lett.271202-2062007-------------5--------2--------2------2-------------------2---------------
690888GorrellInvestigation of the Methanosa ...Methanosarcina thermophilaBiochemistry4614170-141762007--1-5--22-1-1-2--11--1-2----12---1-----11-5----22-1-1---1--1-2----12----------
693619HalbedelTranscription in Mycoplasma pn ...Mycoplasma pneumoniaeJ. Mol. Biol.371596-6072007--1--------1-2--1---1-2--------------1-----------1---1--1-2---------------
672708SuwannakhamConstruction and characterizat ...Propionibacterium acidipropioniciBiotechnol. Bioeng.94383-3952006-------------3--------1--------1------1-------------------1---------------
677237Ingram-SmithAcetate kinase: not just a bac ...Ajellomyces capsulatus, Aspergillus fumigatus, Bacillus subtilis, Borrelia burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Emericella nidulans, Entamoeba histolytica, Enterococcus faecalis, Gibberella zeae, Helicobacter pylori, Hypocrea jecorina, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Phaeosphaeria nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Uncinocarpus reesii, Ustilago maydisTrends Microbiol.14249-2532006-------------40--------40--------60------60-------------------40---------------
659065Ingram-SmithCharacterization of the acetat ...Methanosarcina thermophilaJ. Bacteriol.1872386-23942005---15--27-----3---2----4----21-----------15----27------------4----21----------
659446GorrellStructural and kinetic analyse ...Methanosarcina thermophilaJ. Biol. Chem.28010731-107422005---15-132-----3--------2----13----1------15--1132------------2----13----------
659856Iyer-Acetate kinase from Methanosar ...Methanosarcina thermophilaMethods Biotechnol.17239-2462005-1--1--4-----1--1-----9----2--------1---1----4-------1----9----2----------
659056HasonaPyruvate formate lyase and ace ...Escherichia coliJ. Bacteriol.1867593-76002004----1--------4--------------------------1---------------------------------
642198ItoHighly sensitive simultaneous ...Geobacillus stearothermophilusAnal. Sci.19105-1092003-----------1-2--------1--------------------------1--------1---------------
642195KnorrCloning, expression, and chara ...Lactobacillus sanfranciscensisMicrobiol. Res.156267-2772001--1----4-221-4--1---1-2-111-1--------1-------4-221---1--1-2-111-1---------
642196MilesSite-directed mutational analy ...Methanosarcina thermophilaJ. Biol. Chem.27645059-450642001--1-11--28-3-1-3------9-1--------------1--11----28-3-1------9-1---------------
642197YuPurification and characterizat ...Desulfovibrio vulgaris, Desulfovibrio vulgaris Miyazaki F.J. Biochem.129411-4212001------11-111-4--1---2111----11-------------1-1-111---1--2111----11--------
642193Ingram-SmithThe role of histidines in the ...Methanosarcina thermophilaJ. Biol. Chem.27533765-337702000--1-9-129-----3--1--------------------1--9--1-29-------1--------------------
642194Singh-WissmannIdentification of essential ar ...Methanosarcina thermophilaBiochemistry393671-36772000--1-7-119---1-4--1-----1--------------1--7--1-19---1---1----1---------------
487537BockPurification and characterizat ...Thermotoga maritimaJ. Bacteriol.1811861-18671999-----11-4-611-511-1----171111-11-----------11---4-611-11-1---171111-11--------
642190Lin-Purification and characterizat ...Clostridium thermocellumTetrahedron5415915-159251998-1-----1-211-1--1---1-11111-111-----1--------1-211---1--1-11111-111-------
642191Singh-WissmannIdentification of essential gl ...Methanosarcina thermophilaJ. Bacteriol.1801129-11341998--1-3-221---1-4------1-11-------------1--3--2-21---1------1-11--------------
642188BussCrystallization of acetate kin ...Methanosarcina thermophilaProtein Sci.62659-26621997---1---------3-------------------------1----------------------------------
642189Winzer-Acetate kinase from Clostridiu ...Clostridium acetobutylicumMicrobiology1433279-32861997--1---24-421-1--1---1-71-------------1-----2-4-421---1--1-71--------------
642187Diez-GonzalezThe acetate kinase of Clostrid ...Clostridium acetobutylicum, Clostridium acetobutylicum P262Arch. Microbiol.166418-4201996------13-21--2--1---1-21---41--------------1-3-21----1--1-21---41---------
642184Aceti-Purification and characterizat ...Methanosarcina thermophilaJ. Biol. Chem.24915444-154481988-----2-21411-11-1---1181-11-11---1-------2---21411-1-1--1181-11-11-1------
642185KimInactivation of Acinetobacter ...Acinetobacter calcoaceticusBiochim. Biophys. Acta956103-1091988-----12----1-3--1-----1------------------1-2-----1---1----1---------------
642186Mannens-Purification and immobilizatio ...Desulfovibrio vulgarisBiotechnol. Lett.10563-5681988-----------1-1--1---1-1--------------------------1---1--1-1---------------
642182FoxIsolation and characterization ...Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2J. Biol. Chem.26113487-134971986-----2611--22-4--2----172----2------------2-6-11--22---2---172----2---------
642183VigenschowPurification and properties of ...Rhodopseudomonas palustrisBiol. Chem. Hoppe-Seyler367951-9561986-------11-231-2--1---1281--1-1----1-----------11-231---1--1281--1-1--1------
642181Ichikawa-Purification and properties of ...Propionibacterium freudenreichiiJ. Ferment. Technol.63143-1491985-------6-611-1--1---1-511-1-211--------------6-611---1--1-511-1-211-------
642179HarwoodProperties of acetate kinase i ...Spirochaeta sp.J. Bacteriol.152246-2541982-------11-1142-1--------12----------------------11-1142--------12---------------
642180Suzuki-Acetate kinase from Bacillus s ...Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503Methods Enzymol.90179-18519823--1-----311-2--1---1-51--1-1-1----3---1-------311---1--1-51--1-1-1-------
642178Nishimura-Acetate kinase from Veilonella ...Veillonella parvula, Veillonella parvula 221Methods Enzymol.71311-31619812--------311-2--1---1251-----------2-----------311---1--1251--------------
642177WongInactivation of Escherichia co ...Escherichia coliBiochim. Biophys. Acta615121-1311980-----13----1-2--------1------------------1-3-----1--------1---------------
642174GriffithAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.254442-44619792-----5---11-2--1---1-11----1------2-------5----11---1--1-11----1---------
642175GriffithAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.2546698-670219792-----24---1-3--------1------1-----2-------2-4---1--------1------1--------
642176KahanePurification and properties of ...Acholeplasma laidlawii, Acholeplasma laidlawii oralJ. Bacteriol.137764-7721979-----766-141-3--1---1767---17-------------7-6-6-141---1--1767---17----------
642172NakajimaPurification and properties of ...Geobacillus stearothermophilus, Geobacillus stearothermophilus NCA 1503J. Biochem.84193-2031978---1-12--621-459-1---1-61--1-111--------1-1-2---621-59-1--1-61--1-111-------
642173YoshimuraPurification and characterizat ...Veillonella parvulaArch. Biochem. Biophys.189424-43219782-----94-511-3--1---1251--2-1-1----2-------9-4-511---1--1251--2-1-1-------
642171BowmanAcetate kinase from Veillonell ...Veillonella parvulaJ. Biol. Chem.2513117-312119762------10--11-2------1-21-----------2---------10--11------1-21--------------
642168JansonThe inhibition of acetate, pyr ...Escherichia coliJ. Biol. Chem.2492567-25711974------2--1---1-----------------------------2---1--------------------------
642169JansonThe specificity of chromium nu ...Escherichia coliJ. Biol. Chem.2492572-25741974------1--1---1-----------------------------1---1--------------------------
642170SchauppPurification and properties of ...Moorella thermoaceticaArch. Microbiol.100121-1291974-------1--11-2--1-----1-1--------------------1--11---1----1-1-------------
642167AnthonyExchange reactions catalyzed b ...Escherichia coliJ. Biol. Chem.2466129-61351971---------1-1-1--1----11-----111----------------1-1---1---11-----111-------
642166Rose-Acetate kinase ...Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenesThe Enzymes, 2nd. Ed. (Boyer, P.D., Lardy, H., Myrbaeck, K., eds.)6115-1181962-----134-1-3-3--2---1-7-----1-1----------1-3-4-1-3---2--1-7-----1-1-------