Main physicochemical features of monofunctional flavokinase from Bacillus subtilis

Solovieva, I.M.; Tarasov, K.V.; Perumov, D.A.; Biochemistry 68, 177-181 (2003)

 show all sequences of 2.7.1.26

Data extracted from this reference:

Inhibitors
Inhibitors Commentary Organism Structure
7,8-dimethyl-10-(2'-hydroxyethyl)-isoalloxazine 0.5 mM, 10% inhibition Bacillus subtilis
7,8-dimethyl-10-(O-methylacetoxime)-isoaloxazine 0.5 mM, 34% inhibition Bacillus subtilis
Ca2+
-
 Bacillus subtilis
Co2+
-
 Bacillus subtilis
Lumiflavin 0.5 mM, 4% inhibition Bacillus subtilis
roseoflavin 0.4 mM, 8% inhibition Bacillus subtilis
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.112
-
 ATP pH 8, 37°C Bacillus subtilis
0.18
-
 riboflavin pH 8, 37°C Bacillus subtilis
Metals/Ions
Metals/Ions Commentary Organism Structure
Mg2+ required for maximal activity Bacillus subtilis
Zn2+ less effective in activation than Mg2+ Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
27250
-
 gel filtration Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + riboflavin Bacillus subtilis key enzyme in flavin biosynthesis ADP + FMN
-
-
 ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Bacillus subtilis
-
 monofunctional enzyme
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + riboflavin
-
 641252 Bacillus subtilis ADP + FMN
-
-
-
 ?
ATP + riboflavin key enzyme in flavin biosynthesis 641252 Bacillus subtilis ADP + FMN
-
-
-
 ?
CTP + riboflavin 50% of the activity with ATP 641252 Bacillus subtilis CTP + riboflavin 5'-phosphate
-
-
-
 ?
dATP + riboflavin
-
 641252 Bacillus subtilis dADP + FMN
-
-
-
 ?
UTP + riboflavin 31% of the activity with ATP 641252 Bacillus subtilis UDP + FMN
-
-
-
 ?
Temperature Optimum [°C]
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
52
-
-
 Bacillus subtilis
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Bacillus subtilis
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
7,8-dimethyl-10-(2'-hydroxyethyl)-isoalloxazine 0.5 mM, 10% inhibition Bacillus subtilis
7,8-dimethyl-10-(O-methylacetoxime)-isoaloxazine 0.5 mM, 34% inhibition Bacillus subtilis
Ca2+
-
 Bacillus subtilis
Co2+
-
 Bacillus subtilis
Lumiflavin 0.5 mM, 4% inhibition Bacillus subtilis
roseoflavin 0.4 mM, 8% inhibition Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.112
-
 ATP pH 8, 37°C Bacillus subtilis
0.18
-
 riboflavin pH 8, 37°C Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Mg2+ required for maximal activity Bacillus subtilis
Zn2+ less effective in activation than Mg2+ Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
27250
-
 gel filtration Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + riboflavin Bacillus subtilis key enzyme in flavin biosynthesis ADP + FMN
-
-
 ?
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + riboflavin
-
 641252 Bacillus subtilis ADP + FMN
-
-
-
 ?
ATP + riboflavin key enzyme in flavin biosynthesis 641252 Bacillus subtilis ADP + FMN
-
-
-
 ?
CTP + riboflavin 50% of the activity with ATP 641252 Bacillus subtilis CTP + riboflavin 5'-phosphate
-
-
-
 ?
dATP + riboflavin
-
 641252 Bacillus subtilis dADP + FMN
-
-
-
 ?
UTP + riboflavin 31% of the activity with ATP 641252 Bacillus subtilis UDP + FMN
-
-
-
 ?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
52
-
-
 Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Bacillus subtilis

See also following references to EC number 2.7.1.26 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
703642GiancasperoThe occurrence of riboflavin k ...Nicotiana tabacumFEBS J.276219-2312009-------12----3--------1----------------------12-----------1---------------
705886YazdanpanahRiboflavin kinase couples TNF ...Homo sapiens, Mus musculusNature4601159-11632009--2-2--------5-----2-----------------2--2--------------2-------------22---
687408GrillThe bifunctional flavokinase/f ...Bacillus subtilis, Streptomyces davawensisJ. Bacteriol.1901546-15532008--1----4---1-7--1-----5----4---------1-------4---1---1----5----4----------
679916HigashitsujiRibR, a possible regulator of ...Bacillus subtilisFEMS Microbiol. Lett.27448-542007--1--------1-4--------2--------------1-----------1--------2---------------
679888SolovievaThe riboflavin kinase encoding ...Bacillus subtilisFEMS Microbiol. Lett.24351-582005--1-----2--1-3--------1--------------1--------2--1--------1---------------
680633SandovalAn FMN hydrolase is fused to a ...Arabidopsis thalianaJ. Biol. Chem.28038337-383452005--1----2-----5--------1--------------1-------2------------1---------------
641252SolovievaMain physicochemical features ...Bacillus subtilisBiochemistry68177-1812003------62-211-3--------5-1---1--------------6-2-211--------5-1---1---------
641256BauerCrystal structure of Schizosac ...Schizosaccharomyces pombeJ. Mol. Biol.3261463-14732003---1---------3--------1----------------1------------------1---------------
641257KarthikeyanCrystal structure of human rib ...Homo sapiensStructure11265-2732003---1-------1-3--------2----------------1---------1--------2---------------
657963KarthikeyanLigand binding-induced conform ...Homo sapiensBiochemistry4212532-125382003---1-----1-1-5--11----2----------------1-------1-1---1----2---------------
641251Cho-Bioluminescent assay of bovine ...Bos taurusJ. Microbiol.3874-792000-------2-----1-----11-1-11--11---------------2---------11-1-11--11--------
641249RajeswariPurification and characterizat ...Neurospora crassaIndian J. Biochem. Biophys.36137-14219994------2-211-3--1---1-211--21------4---------2-211---1--1-211--21---------
641254SolovievaThe ribR gene encodes a monofu ...Bacillus subtilisMicrobiology14567-731999--1----------5--------1--------------1--------------------1---------------
641253MackRegulation of riboflavin biosy ...Bacillus subtilisJ. Bacteriol.180950-9551998--1-------11-3--1-----31-------------1----------11---1----31--------------
641250BandyopadhyayEffect of cadmium, mercury and ...Rattus norvegicusMol. Cell. Biochem.16773-801997------6------3--1--21-1--------------------6---------1-21-1---------------
641255BandyopadhyayEffect of cadmium on purified ...Rattus norvegicusLife Sci.601891-19031997------41-----2--1--2--1--------------------4-1-------1-2--1---------------
641235Nakano-Rat brain flavokinase: purific ...Rattus norvegicusFlavins and Flavoproteins (Proc. Int. Symp., 10th, Meeting Date 1990, Curti, B., Ronchi S., Zanetti, G., eds.) de Gruyter, BerlinNew York89-921991-------7-2---1--1--3119-----1----------------7-2-----1-3119-----1---------
641239YamadaProbable reaction mechanisms o ...Rattus norvegicusArch. Biochem. Biophys.278125-1301990------21-----3---1-2--1---------3----------231---------2--1---------------
641240KasaiPurification, properties, and ...Rattus norvegicusJ. Biochem.107298-3031990-----1----1--3-----2111311---1------------1------1------2111311---1---------
641248MansteinPurification and characterizat ...Corynebacterium ammoniagenesJ. Biol. Chem.26116169-161731986-------1-51--2--1-----21---------------------1-51----1----21--------------
641245SobhanadityaPlant flavokinase. Affinity-ch ...Vigna radiata var. radiataBiochem. J.197227-2321981----------1--1--1--11-111---1-------------------1----1-11-111---1---------
641241MerrillAffinity chromatographic purif ...Rattus norvegicusJ. Biol. Chem.2551335-13381980-----1191-21--3--1--21181--------16--------1-19161-21----1-21181--------------
641242MayhewContinuous fluorescence assay, ...Megasphaera elsdeniiMethods Enzymol.66323-3271980-------2-----2--1---1-4--1-------------------2-------1--1-4--1------------
641244MerrillPreparation and properties of ...Rattus norvegicusBiotechnol. Bioeng.211629-16381979-1---1-2-----3-----2--5---1---------1----1---2---------2--5---1-----------
641247KearnyFlavokinase and FAD synthetase ...Bacillus subtilisJ. Biol. Chem.2549551-95571979------71-4---3--1---1-2--------------------7-1-4-----1--1-2---------------
392186WalshChemical and enzymatic propert ...Corynebacterium ammoniagenesBiochemistry171942-19511978-------------4--------7-----------------------------------7---------------
641243MayhewA continuous fluorometric assa ...Megasphaera elsdeniiBiochim. Biophys. Acta482341-3471977-----1-2-----4------1-4--1--11-----------1---2----------1-4--1--11--------
641237ChassyThe effect of the length of th ...Rattus norvegicusJ. Biol. Chem.2401338-13401965------5------1-----1--3---------5----------55----------1--3---------------
641238McCormick-Substrate specificity of liver ...Rattus norvegicusBiochim. Biophys. Acta65326-3321962-------------1-----1--4--------------------------------1--4---------------
641236KearneyThe interaction of yeast flavo ...Saccharomyces cerevisiaeJ. Biol. Chem.194747-7541952-------------2--------4-----------------------------------4---------------