Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum

Lansdon, E.B.; Segel, I.H.; Fisher, A.J.; Biochemistry 41, 13672-13680 (2002)

 show all sequences of 2.7.1.25

Data extracted from this reference:

Crystallization (Commentary)
Crystallization Organism
crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A Penicillium chrysogenum
Inhibitors
Inhibitors Commentary Organism Structure
adenosine 5'-phosphosulfate adenosine 5'-phosphosulfate can bind to E-MgADP forming a catalytically inactive E-MgADP-APS ternary complex Penicillium chrysogenum
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Penicillium chrysogenum Q12657
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-
Reaction
Reaction Commentary Organism
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate enzyme follows a compulsory ordered mechanism in which MgATP2- binds before APS, and PAPS leaves before MgADP- Penicillium chrysogenum
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS 641233 Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate 641233 Penicillium chrysogenum ?
Subunits
Subunits Commentary Organism
dimer 2 * 23670 Penicillium chrysogenum
Crystallization (Commentary) (protein specific)
Crystallization Organism
crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A Penicillium chrysogenum
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
adenosine 5'-phosphosulfate adenosine 5'-phosphosulfate can bind to E-MgADP forming a catalytically inactive E-MgADP-APS ternary complex Penicillium chrysogenum
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS 641233 Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate 641233 Penicillium chrysogenum ?
Subunits (protein specific)
Subunits Commentary Organism
dimer 2 * 23670 Penicillium chrysogenum

See also following references to EC number 2.7.1.25 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
701464GayStructure of the two-domain he ...Thiobacillus denitrificansActa Crystallogr. Sect. D651021-10312009--11---------5--1-----11-------------1-1-------------1----11--------------
705207BradleySulfate activation enzymes: ph ...Phytophthora infestans T30-4J. Mol. Evol.681-132009--1----------1--------1--------------1--------------------1----------11---
674838SekulicStructural mechanism for subst ...Homo sapiensJ. Biol. Chem.28222112-221212007--115-24-3---4--1-----1-1--51---3----1-26--234-3-----2----1-1--51---------
675422YuCrystal structure of the bifun ...Aquifex aeolicusJ. Mol. Biol.365732-7432007---1---------4--1----------------------1-------------1--------------------
675437SekulicElucidation of the active conf ...Homo sapiensJ. Mol. Biol.367488-5002007--11-----3---3--1-----1-1---1--------1-1-------3-----1----1-1---1---------
662642HarjesThe crystal structure of human ...Homo sapiensJ. Mol. Biol.347623-6352005--11-------1-3--1-----21-------------1-1---------1---1----21--------------
660598HarjesExpression, purification and c ...Homo sapiensActa Crystallogr. Sect. D60350-3522004--11------11-4--1-----21-------------1-1--------11---1----21--------------
661125LansdonHuman 3'-phosphoadenosine 5'-p ...Homo sapiensBiochemistry434356-436520041-1---12-211-5--1--11-21--------1--1-1-----112-211---1-11-21--------------
641234VenkatachalamHuman 3'-phosphoadenosine 5'-p ...Caenorhabditis elegans, Cavia porcellus, Danio rerio, Drosophila melanogaster, Homo sapiens, Mus musculus, Takifugu rubripes, Urechis caupoIUBMB Life551-112003----5------2-12-----4--10-----------------5--------2-----8--12---------------
661026XuPharmacogenetics of human 3'-p ...Homo sapiensBiochem. Pharmacol.651787-17962003--1-2------1-3-----1--2--------------1--2--------1-----1--2---------------
662177SinghIdentification and functional ...Mus musculusJ. Biol. Chem.27871-752003--1-15------1-3--1---1-2--------------1--15--------1---1--1-2---------------
641231FudaCharacterization and expressio ...Cavia porcellus, Homo sapiensBiochem. J.365497-5042002-------3-----5-----17--2----3-----------------3---------17--2----3----------
641232ShenThe Xanthomonas oryzae pv. ory ...Sinorhizobium meliloti, Xanthomonas oryzaeMol. Microbiol.4437-482002--1--------1-2--------3--------------1-----------1--------3---------------
641233LansdonLigand-induced structural chan ...Penicillium chrysogenumBiochemistry4113672-136802002---1--1------3---1----11---------------1---1--------------11--------------
641230LilligMolecular and catalytic proper ...Arabidopsis thalianaArch. Biochem. Biophys.392303-31020013-1-21221----4--------11--------1--3-1--21-2121-----------11--------------
641228SatishchandranMechanistic studies of Escheri ...Escherichia coliArch. Biochem. Biophys.378210-2152000-------4-3---3--11----3----1-----------------4-3-----1----3----1----------
641229XuHuman 3'-phosphoadenosine 5'-p ...Homo sapiensBiochem. Biophys. Res. Commun.268437-4442000------1-1----3-----2--1--------------------1--1--------2--1---------------
641225DeyrupActivity and stability of reco ...Mus musculusJ. Biol. Chem.27410751-107571999--1----------2------1-1---1----------1------------------1-1---1-----------
641226MacRaeAdenosine 5'-phosphosulfate (A ...Penicillium chrysogenumArch. Biochem. Biophys.361277-2821999------12-----3--------1---------1----------112------------1---------------
641227VenkatachalamSite-selected mutagenesis of a ...Homo sapiensJ. Biol. Chem.2742601-26041999--1-5--------3--1-----1--------------1--5------------1----1---------------
641222LeeAPS kinase from Arabidopsis th ...Arabidopsis thalianaBiochem. Biophys. Res. Commun.247171-1751998--1---121----3-----2-111-------------1-----1-21--------2-111--------------
641223MacRaeAdenosine 5'-phosphosulfate ki ...Escherichia coli, Penicillium chrysogenumJ. Biol. Chem.27328583-2858919981-1-7-16-----5--------3------------1-1--7--1-6------------3---------------
641224VenkatachalamMolecular cloning, expression, ...Homo sapiensJ. Biol. Chem.27319311-193201998--1----7-----4--------2--------------1-------7------------2---------------
641221Onda-Purification and characterizat ...Geobacillus stearothermophilus, Saccharomyces cerevisiaeBiosci. Biotechnol. Biochem.60134-1361996------22-41--2--1---1-22--4-2--------------2-2-41----1--1-22--4-2---------
641220RosenthalA multifunctional Urechis caup ...Rattus norvegicus, Urechis caupoGene165243-2481995--1----------7-----3--31-------------1-----------------3--31--------------
641216SatishchandranCharacterization of the phosph ...Escherichia coliBiochemistry3111684-116881992--1--------1-3-111----31-------------1-----------1--11----31--------------
641206RenostoATP sulfurylase from trophosom ...Riftia pachyptilaArch. Biochem. Biophys.29066-781991---------1---1-----1--1-1----------------------1-------1--1-1-------------
641211Kanno-Purification and properties of ...Porphyra yezoensisBot. Mar.33369-3741990------11-11--1--1--1--41----1--------------1-1-11----1-1--41----1---------
641204YuRat liver ATP-sulfurylase: pur ...Rattus norvegicusArch. Biochem. Biophys.269156-1741989---------1---3-----1--1------------------------1-------1--1---------------
641210RenostoSulfate-activating enzymes of ...Penicillium chrysogenumJ. Biol. Chem.2649433-94371989-------1-1---3---1-1--2----------------------1-1-------1--2---------------
641215SatishchandranAdenosine-5-phosphosulfate kin ...Escherichia coliJ. Biol. Chem.26415012-150211989------85-52--1--11--1-221--11---1----------815-52----1--1-221--11---------
641205HommesPurification and some properti ...Rattus norvegicusBiochim. Biophys. Acta924270-27519871----1211-1--3--1--21-11----11-----1-----1-2-11-1----1-21-11----11--------
641217SchriekProperties of the purified APS ...Escherichia coli, Saccharomyces cerevisiaeArch. Microbiol.14532-38198610----218-2-4--5--2---4-23----64-----10-----2-18--2-4----2--4-23----64--------
641208RenostoComparative stability and cata ...Penicillium chrysogenum, Penicillium dupontiJ. Bacteriol.164674-6831985---------22--6--2--2--21--1--------------------22----2-2--21--1-----------
641209RenostoAPS kinase from Penicillium ch ...Penicillium chrysogenumJ. Biol. Chem.2601535-15441985-----12--11--3-----11-111-6---2----------1-2---11------11-111-6---2-------
641207RenostoAdenosine 5-phosphosulfate kin ...Penicillium chrysogenumJ. Biol. Chem.2592113-21231984------44-311-3--1--11-211-1-1--------------4-4-311---1-11-211-1-1---------
641213Schwenn-Purification and properties of ...Chlamydomonas reinhardtiiArch. Microbiol.1389-1419844----2102-4---1--1---11511---43-----4-----2-10-2-4-----1--11511---43--------
641214Schwenn-A new role for thioredoxin in ...Chlamydomonas reinhardtiiFEBS Lett.17076-8019841------------1--------1-1----------1----------------------1-1-------------
641212Schwenn-A kinetic investigation of the ...Chlamydomonas reinhardtiiPhytochemistry20601-6041981------22-1---1--1---1-1--------------------2-2-1-----1--1-1---------------
641218Bandurski- ...Saccharomyces cerevisiaeJ. Am. Chem. Soc.786408-64091956-------------1--------1-----------------------------------1---------------
641219Robbins- ...Saccharomyces cerevisiaeJ. Am. Chem. Soc.786409-64101956---------2---1--1---1-1------------------------2-----1--1-1---------------