Purification and properties of a diamine alpha-ketoglutarate transaminase from Escherichia coli

Kim, K.H.; J. Biol. Chem. 239, 783-786 (1964)

Data extracted from this reference:

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.88	-	2-oxoglutarate	pH 7.6, room temperature	Escherichia coli
2.7	-	pyruvate	pH 7.6, room temperature	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
putrescine + 2-oxoglutarate	Escherichia coli	-	4-aminobutanal + L-glutamate	-	Escherichia coli	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Commentary	Organism
precipitation with ammonium sulphate followed by column chromatography	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
1.63	-	-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,7-diaminoheptane + 2-oxoglutarate	30% of the activity with putrescine	639950	Escherichia coli	7-aminoheptanal + L-glutamate	-	639950	Escherichia coli	?
4-aminobutanoate + 2-oxoglutarate	11% of the activity with putrescine	639950	Escherichia coli	4-oxobutanoate + L-glutamate	-	639950	Escherichia coli	?
cadaverine + 2-oxoglutarate	equally active as putrescine	639950	Escherichia coli	5-aminopentanal + L-glutamate	-	639950	Escherichia coli	?
More	inactive with oxaloacetate	639950	Escherichia coli	?	-	-	-	-
More	inactive with 1,3-diaminopropane, lysine, ornithine, spermidine	639950	Escherichia coli	?	-	-	-	-
putrescine + 2-oxoglutarate	-	639950	Escherichia coli	4-aminobutanal + L-glutamate	-	639950	Escherichia coli	?
putrescine + pyruvate	-	639950	Escherichia coli	4-aminobutanal + L-alanine	-	639950	Escherichia coli	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
9	10	-	Escherichia coli

pH Range

pH Minimum	pH Maximum	Commentary	Organism
8	10.5	almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity	Escherichia coli

Cofactor

Cofactor	Commentary	Organism	Structure
pyridoxal 5'-phosphate	required	Escherichia coli

Cofactor (protein specific)

Cofactor	Commentary	Organism	Structure
pyridoxal 5'-phosphate	required	Escherichia coli

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.88	-	2-oxoglutarate	pH 7.6, room temperature	Escherichia coli
2.7	-	pyruvate	pH 7.6, room temperature	Escherichia coli

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
putrescine + 2-oxoglutarate	Escherichia coli	-	4-aminobutanal + L-glutamate	-	Escherichia coli	?

Purification (Commentary) (protein specific)

Commentary	Organism
precipitation with ammonium sulphate followed by column chromatography	Escherichia coli

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
1.63	-	-	Escherichia coli

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,7-diaminoheptane + 2-oxoglutarate	30% of the activity with putrescine	639950	Escherichia coli	7-aminoheptanal + L-glutamate	-	639950	Escherichia coli	?
4-aminobutanoate + 2-oxoglutarate	11% of the activity with putrescine	639950	Escherichia coli	4-oxobutanoate + L-glutamate	-	639950	Escherichia coli	?
cadaverine + 2-oxoglutarate	equally active as putrescine	639950	Escherichia coli	5-aminopentanal + L-glutamate	-	639950	Escherichia coli	?
More	inactive with oxaloacetate	639950	Escherichia coli	?	-	-	-	-
More	inactive with 1,3-diaminopropane, lysine, ornithine, spermidine	639950	Escherichia coli	?	-	-	-	-
putrescine + 2-oxoglutarate	-	639950	Escherichia coli	4-aminobutanal + L-glutamate	-	639950	Escherichia coli	?
putrescine + pyruvate	-	639950	Escherichia coli	4-aminobutanal + L-alanine	-	639950	Escherichia coli	?

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
9	10	-	Escherichia coli

pH Range (protein specific)

pH Minimum	pH Maximum	Commentary	Organism
8	10.5	almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity	Escherichia coli

See also following references to EC number 2.6.1.29 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
639952	Bascarán		Stringent response and initiat ...	Streptomyces clavuligerus	J. Gen. Microbiol.	137	1625-1634	1991	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
639953	Madduri		Lysine catabolism in Streptomy ...	Amycolatopsis lactamdurans, Streptomyces clavuligerus, Streptomyces glaucescens, Streptomyces griseus, Streptomyces lividans, Streptomyces parvulus, Streptomyces phaeochromogenes, Streptomyces rimosus, Streptomyces venezuelae, Streptomyces viridochromogenes	J. Bacteriol.	171	299-302	1989	-	-	-	-	-	-	-	-	-	-	-	10	-	18	-	-	-	-	-	-	10	-	20	-	10	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	10	-	-	-	-	-	-	10	-	20	-	10	-	-	-	-	-	-	-	-	-	-	-	-	-
639951	Kim	-	Diamine-alpha-ketoglutarate am ...	Escherichia coli	Methods Enzymol.	17B	812-815	1971	-	-	-	-	-	-	3	2	-	-	-	1	-	1	-	-	1	-	-	-	1	1	9	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	1	-	-	-	-	3	-	2	-	-	-	1	-	-	-	1	-	-	1	1	9	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
639950	Kim		Purification and properties of ...	Escherichia coli	J. Biol. Chem.	239	783-786	1964	-	-	-	-	-	-	-	2	-	-	-	1	-	1	-	-	1	-	-	-	1	-	7	-	-	-	-	-	1	1	-	1	-	-	-	-	-	-	1	-	-	-	-	-	-	2	-	-	-	1	-	-	-	1	-	-	1	-	7	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-