Disulfide bonds of GM2 synthase homodimers. Antiparallel orientation of the catalytic domains

Li, J.; Yen, T.Y.; Allende, M.L.; Joshi, R.K.; Cai, J.; Pierce, W.M.; Jaskiewicz, E.; Darling, D.S.; Macher, B.A.; Young, W.W., Jr.; J. Biol. Chem. 275, 41476-41486 (2000)

 show all sequences of 2.4.1.92

Data extracted from this reference:

Engineering
Amino acid exchange Commentary Organism
additional information site directed mutations in several Cys residues reveal that enzyme is homodimer with antiparallel orientation of catalytic domains and intersubunit disulfide bonds Cricetulus griseus
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Cricetulus griseus
-
-
-
Source Tissue
Source Tissue Commentary Organism Textmining
ovary
-
 Cricetulus griseus
-
Subunits
Subunits Commentary Organism
More enzyme is homodimer with antiparallel orientation of catalytic domains and intersubunit disulfide bonds Cricetulus griseus
Engineering (protein specific)
Amino acid exchange Commentary Organism
additional information site directed mutations in several Cys residues reveal that enzyme is homodimer with antiparallel orientation of catalytic domains and intersubunit disulfide bonds Cricetulus griseus
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
ovary
-
 Cricetulus griseus
-
Subunits (protein specific)
Subunits Commentary Organism
More enzyme is homodimer with antiparallel orientation of catalytic domains and intersubunit disulfide bonds Cricetulus griseus

See also following references to EC number 2.4.1.92 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
705475ChenAnalysis of GD2/GM2 synthase m ...Homo sapiensLung Cancer67216-2202010-1-----------3-----10----------------1------------------10------------------
706531OhmiGangliosides play pivotal role ...Mus musculusProc. Natl. Acad. Sci. USA10622405-224102009-------------3-------------------------------------------------------11---
686976WangExpression of the human Sda be ...Homo sapiensGlycobiology18104-11320081------------1-----4--1------------1-------------------4--1---------------
688514FurukawaDisruption of GM2/GD2 synthase ...Mus musculusJ. Neurochem.1051057-10662008-------------5-----3-----------------------------------3------------------
702508OotsukaUseful markers for detecting m ...Homo sapiensBiol. Pharm. Bull.311071-10742008-------------3-----10--1--------------------------------10--1---------------
703052PukinGM3, GM2 and GM1 mimics design ...Campylobacter jejuniCarbohydr. Res.343636-6502008-------------1--------3-----------------------------------3---------------
704993LiGM2/GD2 and GM3 gangliosides h ...Mus musculusJ. Lipid Res.491816-18282008-------------3-------------------------------------------------------11---
671792SasakiDrosophila beta1,4-N-acetylgal ...Drosophila melanogasterBiochem. Biophys. Res. Commun.354522-5272007--1-1----1-1-3--1--11-3-1---1--------1--1------1-1---1-11-3-1---1---------
673868HainesFunctional roles for beta1,4-N ...Drosophila melanogasterGenetics175671-6792007----1--------2-----3--1-----------------1--------------3--1---------------
675421RamakrishnanRole of a single amino acid in ...Drosophila melanogaster, Homo sapiensJ. Mol. Biol.365570-5762007----2------2-6---2----3-----------------2--------2--------3---------------
685784MartinezHuman bone marrow mesenchymal ...Homo sapiensBlood1094245-42482007-1-----------3-----5----------------1------------------5------------------
676275JacquesChemo-enzymatic synthesis of G ...Campylobacter jejuniOrg. Biomol. Chem.4142-1542006-11--------3-2--------4-------------11-----------3--------4---------------
658854HainesFunctional analysis of Drosoph ...Drosophila melanogasterGlycobiology15335-3462005----1--------2--------------------------1---------------------------------
659454KawarNovel poly-GalNAcbeta1-4GlcNAc ...Caenorhabditis elegansJ. Biol. Chem.28012810-128192005-1-----------6--------2-------------1---------------------2---------------
658735GotohMolecular cloning and characte ...Homo sapiensFEBS Lett.562134-1402004--1----------1-1---4--51-------------1--------------1--4--51--------------
659370VadaieMolecular cloning and function ...Trichoplusia niJ. Biol. Chem.27933501-335182004-------21----1--------4----------------------21-----------4---------------
657819ArdailThe mitochondria-associated en ...Rattus norvegicusBiochem. J.3711013-10192003--------1----1-----1--------------------------1--------1------------------
659264GiraudoGanglioside glycosyltransferas ...Cricetulus griseusJ. Biol. Chem.27840262-402712003--------1----2-----1---1----------------------1--------1---1--------------
659865WendelerRecombinant ganglioside GM2 sy ...Mus musculusMethods Enzymol.363476-4892003-1-----31-1--1------2-11------------1--------31-1-------2-11--------------
489583BieberichRegulation of ganglioside bios ...Mus musculusBiochemistry4111479-114872002----1--------3---------1----------------1------------------1--------------
489589Furukawabeta1,4-N-acetylgalactosaminyl ...Homo sapiens, Mus musculusBiochim. Biophys. Acta1573356-3622002----2---1----5-1------3-----------------2-----1-----1-----3---------------
489591LiThe DXD motif is required for ...Homo sapiensGlycobiology11217-2292001----7--------2--------------------------7---------------------------------
489584LiDisulfide bonds of GM2 synthas ...Cricetulus griseusJ. Biol. Chem.27541476-414862000----1--------2-----1---1----------------1--------------1---1--------------
489590DaniottiGM3 alpha2,8-sialyltransferase ...Homo sapiensJ. Neurochem.741711-17202000--------1----3---------1----------------------1------------1--------------
489586Zhubeta1,4 N-Acetylgalactosaminyl ...Homo sapiensGlycobiology7987-9961997----1---1----2-1-------1----------------1-----1-----1------1--------------
489585JaskiewiczCloned beta1,4N-acetylgalactos ...Homo sapiensGlycoconjugate J.13213-2231996----1---1----3--------------------------1-----1---------------------------
489587Haraguchi-The effects of the site-direct ...Homo sapiensBiochem. J.312273-2801995----1--------1--------------------------1---------------------------------
489588RuanAnalysis of melanoma cells sta ...Homo sapiensArch. Biochem. Biophys.32311-181995-----------1-1--------1--------------------------1--------1---------------
489578HashimotoPurification and characterizat ...Mus musculusJ. Biol. Chem.26825857-2586419933----1-416---1--1--112611---1------3-----1---416-----1-112611---1---------
489577NagataExpression cloning of beta 1,4 ...Homo sapiensJ. Biol. Chem.26712082-120891992--1----------3-----2-----------------1-----------------2------------------
489579PohlentzBoth GA2, GM2, and GD2 synthas ...Rattus norvegicusProc. Natl. Acad. Sci. USA857044-704819882-----211--1-1---1-1--4-1----------2-------2-11--1-----1--4-1-------------
489581NagaiBiosynthesis of monosulfogangl ...Rattus norvegicusJ. Biochem.1011115-112719871------4-4---1-----1-13-----11-----1---------4-4-------1-13-----11--------
489582YanagisawaPurification and properties of ...Rattus norvegicusBiochim. Biophys. Acta919213-2201987-----131-81--2--1--21151----1------------1-3-1-81----1-21151----1---------
587166KleinSubstrate specificity of GM2 a ...Rattus norvegicusEur. J. Biochem.167417-42419873-------1----3-----1--2-1----------3----------1--------1--2-1-------------
587167YusufOligosialogangliosides inhibit ...Rattus norvegicusBiol. Chem. Hoppe-Seyler368455-46219874-----5-1----1-----1--1-1----------4-------5--1--------1--1-1-------------
489573ScheidelerDirect demonstration of the ac ...Rattus norvegicusJ. Neurochem.461639-16431986-------------1-----2-----------------------------------2------------------
489575SennGanglioside biosynthesis in ra ...Homo sapiens, Rattus norvegicusEur. J. Biochem.12059-6719814-----3218---3-----21-2-----1------4-------3-218-------21-2-----1---------
489574DicesareThe enzymic synthesis of gangl ...Rattus norvegicusBiochim. Biophys. Acta231385-39319713------225-1-1-----1--7-1---1------3---------225-1-----1--7-1---1---------