A high-throughput pH indicator assay for screening glycosyltransferase saturation mutagenesis libraries

Persson, M.; Palcic, M.M.; Anal. Biochem. 378, 1-7 (2008)

Data extracted from this reference:

Engineering

Amino acid exchange	Commentary	Organism
M214G	saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB	Homo sapiens
M214S	saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB	Homo sapiens
additional information	screening of a saturation mutagenesis library for mutant determination with altered cosubstrate specificity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens	-

Localization

Localization	Commentary	Organism	GeneOntology No.	Textmining
cell surface	-	Homo sapiens	9986	-

Metals/Ions

Metals/Ions	Commentary	Organism	Structure
Mn2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	Homo sapiens	-	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Commentary	Organism	Textmining
erythrocyte	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
0.05	-	wild-type enzyme, with cosubstrate UDP-GalNAc	Homo sapiens
0.11	-	mutant M214G, with cosubstrate UDP-GalNAc	Homo sapiens
0.14	-	mutant M214S, with cosubstrate UDP-GalNAc	Homo sapiens
1.9	-	mutant M214S, with cosubstrate UDP-Gal	Homo sapiens
3.3	-	wild-type enzyme, with cosubstrate UDP-Gal	Homo sapiens
4.4	-	mutant M214G, with cosubstrate UDP-Gal	Homo sapiens

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	development and evaluation of a general screening assay method for glycosyltransferase activities, overview	690409	Homo sapiens	?	-	-	-	-
UDP-galactose + alpha-L-fucosyl-(1,2)-beta-D-galactosyl-O-(CH2)7CH3	i.e. alpha-L-Fucp-(1,2)-beta-DGalp-O-(CH2)7CH3	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-beta-D-galactosyl-O-(CH2)7CH3	-	-	-	?
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	-	?
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	importance of residue M214 for donor enzyme specificity	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	-	?
UDP-galactose + L-2'-L-fucosyllactose	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-D-glucose	-	-	-	?
UDP-N-acetylgalactosamine + L-2'-fucosyllactose	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-D-glucose	-	-	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.37	-	UDP-Gal	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
0.7	-	UDP-Gal	pH 7.0, 37°C, recombinant mutant M214S	Homo sapiens
0.74	-	UDP-Gal	pH 7.0, 37°C, recombinant mutant M214G	Homo sapiens
4	-	UDP-GalNAc	pH 7.0, 37°C, recombinant mutant M214G	Homo sapiens
5	-	UDP-GalNAc	pH 7.0, 37°C, recombinant mutant M214S	Homo sapiens
5.1	-	UDP-GalNAc	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7	7.3	assay at	Homo sapiens

Engineering (protein specific)

Amino acid exchange	Commentary	Organism
M214G	saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB	Homo sapiens
M214S	saturation mutagenesis of GTB enzyme at M214 leads to a two-fold higher kcat for UDP-GalNAc and specific activity of the mutant compared to the wild-type GTB	Homo sapiens
additional information	screening of a saturation mutagenesis library for mutant determination with altered cosubstrate specificity	Homo sapiens

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens	-

Localization (protein specific)

Localization	Commentary	Organism	GeneOntology No.	Textmining
cell surface	-	Homo sapiens	9986	-

Metals/Ions (protein specific)

Metals/Ions	Commentary	Organism	Structure
Mn2+	-	Homo sapiens

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	Homo sapiens	-	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	?

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
erythrocyte	-	Homo sapiens	-

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
0.05	-	wild-type enzyme, with cosubstrate UDP-GalNAc	Homo sapiens
0.11	-	mutant M214G, with cosubstrate UDP-GalNAc	Homo sapiens
0.14	-	mutant M214S, with cosubstrate UDP-GalNAc	Homo sapiens
1.9	-	mutant M214S, with cosubstrate UDP-Gal	Homo sapiens
3.3	-	wild-type enzyme, with cosubstrate UDP-Gal	Homo sapiens
4.4	-	mutant M214G, with cosubstrate UDP-Gal	Homo sapiens

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	development and evaluation of a general screening assay method for glycosyltransferase activities, overview	690409	Homo sapiens	?	-	-	-	-
UDP-galactose + alpha-L-fucosyl-(1,2)-beta-D-galactosyl-O-(CH2)7CH3	i.e. alpha-L-Fucp-(1,2)-beta-DGalp-O-(CH2)7CH3	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-beta-D-galactosyl-O-(CH2)7CH3	-	-	-	?
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	-	?
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactosyl-R	importance of residue M214 for donor enzyme specificity	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-R	-	-	-	?
UDP-galactose + L-2'-L-fucosyllactose	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactosyl-D-glucose	-	-	-	?
UDP-N-acetylgalactosamine + L-2'-fucosyllactose	-	690409	Homo sapiens	UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactosyl-D-glucose	-	-	-	?

Temperature Optimum [°C] (protein specific)

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s] (protein specific)

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.37	-	UDP-Gal	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
0.7	-	UDP-Gal	pH 7.0, 37°C, recombinant mutant M214S	Homo sapiens
0.74	-	UDP-Gal	pH 7.0, 37°C, recombinant mutant M214G	Homo sapiens
4	-	UDP-GalNAc	pH 7.0, 37°C, recombinant mutant M214G	Homo sapiens
5	-	UDP-GalNAc	pH 7.0, 37°C, recombinant mutant M214S	Homo sapiens
5.1	-	UDP-GalNAc	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7	7.3	assay at	Homo sapiens

See also following references to EC number 2.4.1.37 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
703919	Sindhuwinata		Binding of an acceptor substra ...	Homo sapiens	Glycobiology	20	718-723	2010	1	-	1	-	-	-	-	-	-	2	-	2	-	3	-	-	1	-	-	1	-	-	3	-	1	-	-	-	1	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	2	-	2	-	-	-	1	-	1	-	-	3	-	1	-	-	-	1	-	-	-	-	1	1	-	-	-
703890	Soya		Comparative study of substrate ...	Homo sapiens	Glycobiology	19	1224-1234	2009	-	-	1	-	1	-	-	1	-	1	-	2	-	1	-	-	-	-	-	-	-	-	5	1	1	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	1	-	1	-	2	-	-	-	-	-	-	-	-	5	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-
706853	Yamamoto		Generation of histo-blood grou ...	Homo sapiens	Transfusion	50	622-630	2009	-	-	1	-	1	-	-	-	-	-	-	2	-	4	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
690409	Persson		A high-throughput pH indicator ...	Homo sapiens	Anal. Biochem.	378	1-7	2008	-	-	-	-	3	-	-	1	1	1	-	1	-	1	-	-	-	-	-	1	6	-	6	-	1	-	-	6	1	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	1	1	1	-	1	-	-	-	-	-	1	6	-	6	-	1	-	-	6	1	-	-	-	-	-	-	-	-	-
693025	Alfaro		ABO(H) blood group A and B gly ...	Homo sapiens	J. Biol. Chem.	283	10097-10108	2008	-	-	1	1	4	-	-	-	1	1	-	1	-	1	-	-	1	-	-	1	-	-	2	1	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	1	4	-	-	-	-	-	1	1	-	1	-	-	-	1	-	1	-	-	2	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
706852	Seltsam		Aberrant intracellular traffic ...	Homo sapiens	Transfusion	48	1898-1905	2008	-	-	1	-	1	-	-	-	-	-	-	1	-	2	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
674907	Persson		Structural effects of naturall ...	Homo sapiens	J. Biol. Chem.	282	9564-9570	2007	-	-	-	-	3	-	-	-	-	1	-	-	-	3	-	-	-	-	-	1	-	-	1	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-
690230	Letts		The effect of heavy atoms on t ...	Homo sapiens	Acta Crystallogr. Sect. D	63	860-865	2007	-	-	-	1	1	-	-	-	1	1	-	-	-	3	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
695221	Hosseini-Maaf		Structural basis for red cell ...	Homo sapiens	Transfusion	47	864-875	2007	-	-	1	1	6	-	-	5	1	-	-	1	-	8	-	-	-	-	-	2	-	-	1	1	-	-	-	4	-	-	-	-	-	-	-	-	-	6	-	6	36	-	-	-	-	30	6	-	-	6	-	-	-	-	-	7	-	-	6	6	-	-	-	24	-	-	-	-	-	-	-	-	-	-
674182	Angulo		Blood group B galactosyltransf ...	Homo sapiens	J. Am. Chem. Soc.	128	13529-13538	2006	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
674682	Blume		Fragment-based screening of th ...	Homo sapiens	J. Biol. Chem.	281	32728-32740	2006	-	-	-	-	-	-	-	-	-	3	-	-	-	3	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
674703	Letts		Differential recognition of th ...	Homo sapiens	J. Biol. Chem.	281	3625-3632	2006	-	-	-	1	-	-	-	3	-	-	-	-	-	1	-	-	-	-	-	-	-	-	3	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-
676942	Nakahara		Computational design and exper ...	Homo sapiens	Protein Eng. Des. Sel.	19	571-578	2006	-	-	-	-	4	-	-	6	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-
660551	Yazer		Amino-acid substitution in the ...	Homo sapiens	Transfusion	45	1178-1182	2005	-	-	1	-	2	-	-	1	-	1	-	-	-	5	-	-	1	-	-	1	-	-	1	-	1	-	-	4	1	-	-	-	-	-	-	-	-	1	-	-	2	-	-	-	-	1	-	1	-	-	-	-	-	1	-	1	-	-	1	-	1	-	-	4	1	-	-	-	-	-	-	-	-	-
659358	Ketcham		Specificity of a soluble UDP-g ...	Dictyostelium discoideum	J. Biol. Chem.	279	29050-29059	2004	2	-	-	-	-	-	3	9	3	4	-	1	-	1	-	-	1	-	-	-	2	-	9	-	1	-	-	-	1	-	-	-	-	-	-	2	-	-	-	-	-	-	-	3	-	9	3	4	-	1	-	-	-	1	-	-	2	-	9	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
489114	Marcus		A single point mutation revers ...	Homo sapiens	J. Biol. Chem.	278	12403-12405	2003	-	-	1	1	1	-	-	4	-	1	-	-	-	1	-	-	-	-	-	-	-	-	2	-	1	-	-	2	-	-	-	-	-	-	-	-	-	1	-	1	1	-	-	-	-	4	-	1	-	-	-	-	-	-	-	-	-	-	2	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-
658843	Turcot		Cloning of a rat gene encoding ...	Rattus norvegicus	Glycobiology	13	919-928	2003	-	-	1	-	-	-	-	-	-	-	-	-	-	5	-	-	-	1	-	6	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	6	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
659283	Nguyen		The influence of an intramolec ...	Homo sapiens	J. Biol. Chem.	278	49191-49195	2003	-	-	-	1	-	-	2	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489115	Patenaude		The structural basis for speci ...	Homo sapiens	Nat. Struct. Biol.	9	685-690	2002	-	-	1	1	-	-	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489116	Yamamoto		Murine equivalent of the human ...	Mus musculus brevirostris, Mus musculus castaneus, Mus musculus domesticus, Mus spicilegus	J. Biol. Chem.	276	13701-13708	2001	-	-	4	-	-	-	-	-	-	-	-	-	-	11	-	-	-	-	-	4	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489117	Sujino	-	Enzymatic synthesis of oligosa ...	Homo sapiens	J. Am. Chem. Soc.	122	1261-1269	2000	-	-	-	-	-	-	1	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	2	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489118	Seto		Enzymatic synthesis of blood g ...	Homo sapiens	Carbohydr. Res.	324	161-169	2000	-	-	1	-	-	-	-	2	-	-	-	-	-	3	-	-	-	-	-	-	-	-	2	-	-	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-
489119	Mukherjee		Synthesis and enzymatic evalua ...	Homo sapiens	Carbohydr. Res.	326	1-21	2000	-	-	-	-	-	-	-	4	-	-	-	-	-	3	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489120	Kamath	-	Synthesis of the acceptor anal ...	Homo sapiens	Glycoconjugate J.	16	599-606	2000	-	-	1	-	-	-	-	8	-	-	-	-	-	1	-	-	-	-	-	-	-	-	4	-	1	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	4	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-
489121	Seto		Donor substrate specificity of ...	Homo sapiens	Eur. J. Biochem.	259	770-775	1999	-	-	1	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-
489122	Yu		The molecular basis for the B( ...	Homo sapiens	Biochem. Biophys. Res. Commun.	262	487-493	1999	-	-	-	-	-	-	-	-	-	1	-	-	-	3	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489123	Seto		Sequential interchange of four ...	Homo sapiens	J. Biol. Chem.	272	14133-14138	1997	-	-	1	-	-	-	-	4	-	-	-	-	-	4	-	-	-	-	-	2	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	2	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-
489124	Seto		Expression of a recombinant hu ...	Homo sapiens	Eur. J. Biochem.	234	323-328	1995	-	-	1	-	-	-	3	2	-	-	-	-	-	4	-	-	-	-	-	3	1	-	4	-	-	-	-	-	-	-	-	-	2	-	-	-	-	1	-	-	-	-	-	3	2	2	-	-	-	-	-	-	-	-	-	3	1	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489125	Lowary		Recognition of synthetic O-met ...	Homo sapiens	Carbohydr. Res.	251	33-67	1994	-	-	-	-	-	-	2	3	-	-	-	-	-	2	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	2	1	3	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489126	Nakajima		Blood group A and B glycosyltr ...	Homo sapiens, Hylobates lar entelloides, Macaca fuscata	Exp. Clin. Immunogenet.	10	21-30	1993	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	3	-	-	3	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	3	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-
489127	Yazawa		An enzyme-linked immunosorbent ...	Homo sapiens	Carbohydr. Res.	239	329-335	1993	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
489128	Betteridge		Two alpha-3-D-galactosyltransf ...	Oryctolagus cuniculus	Eur. J. Biochem.	132	29-35	1983	-	-	-	-	-	-	-	4	1	1	-	-	-	1	-	-	-	-	-	1	-	-	4	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	4	1	1	-	-	-	-	-	-	-	1	-	-	4	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-
489129	Nagai		Human blood group glycosyltran ...	Homo sapiens	J. Biol. Chem.	253	380-381	1978	-	-	-	-	-	1	-	-	-	1	1	-	-	1	-	-	1	-	-	1	1	1	1	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	1	-	-	-	-	1	-	1	1	1	1	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-
489130	Carne		Human blood group B gene-speci ...	Homo sapiens	Biochem. Biophys. Res. Commun.	77	700-707	1977	-	-	-	-	-	-	2	4	-	2	-	-	-	1	-	-	1	-	-	2	1	-	5	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	4	-	2	-	-	-	-	-	1	-	2	1	-	5	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-
489131	Race		An alpha-D-galactosyltransfera ...	Homo sapiens, Papio sp.	Biochem. J.	107	733-735	1968	-	-	-	-	-	-	-	-	-	-	-	2	-	2	-	-	-	-	-	4	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	4	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-