Lactobacillus helveticus glycosyltransferases: from genes to carbohydrate synthesis

Jolly, L.; Newell, J.; Porcelli, I.; Vincent, S.J.F.; Stingele, F.; Glycobiology 12, 319-327 (2002)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis	Lactobacillus helveticus

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Lactobacillus helveticus	-	gene epsF; strain NCC2745	-
Lactobacillus helveticus NCC2745	-	strain NCC2745	-

Purification (Commentary)

Commentary	Organism
recombinant as His-tagged or GST-tagged proteins from Escherichia coli	Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	assay development	Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester	638251	Lactobacillus helveticus	?	-	-	-	-
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose	synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37	638251	Lactobacillus helveticus	UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose	-	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	substrate specificity	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?

Cloned(Commentary) (protein specific)

Commentary	Organism
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis	Lactobacillus helveticus

Purification (Commentary) (protein specific)

Commentary	Organism
recombinant as His-tagged or GST-tagged proteins from Escherichia coli	Lactobacillus helveticus

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	assay development	Lactobacillus helveticus

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester	638251	Lactobacillus helveticus	?	-	-	-	-
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose	synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37	638251	Lactobacillus helveticus	UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose	-	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	substrate specificity	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n	enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain	638251	Lactobacillus helveticus	UDP + [alpha-D-glucosyl-(1-3)]n+1	product analysis	638251	Lactobacillus helveticus	?

See also following references to EC number 2.4.1.183 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
673818	Maubon		AGS3, an a(1-3)glucan synthase ...	Aspergillus fumigatus	Fungal Genet. Biol.	43	366-375	2006	-	-	1	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
658806	Damveld		Expression of agsA, one of fiv ...	Aspergillus niger, Penicillium chrysogenum	Fungal Genet. Biol.	42	165-177	2005	-	-	2	-	-	-	-	-	-	-	-	3	-	11	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
676055	Damveld		The Aspergillus niger MADS-box ...	Aspergillus niger	Mol. Microbiol.	58	305-319	2005	-	-	-	-	-	-	-	-	1	-	-	-	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
638251	Jolly		Lactobacillus helveticus glyco ...	Lactobacillus helveticus	Glycobiology	12	319-327	2002	-	-	1	-	-	-	-	-	-	-	-	-	-	2	-	-	1	-	-	-	1	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
638250	Yamashita		Evidence for the presence of t ...	Streptococcus mutans	FEBS Lett.	243	343-346	1989	-	-	-	-	-	-	1	-	-	-	-	1	-	3	-	-	1	-	-	-	-	-	4	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	1	-	-	-	-	4	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-