Purification and characterization of the plant glutaminyl-peptide cyclotransferase isolated from papaya latex

Zerhouni, S.; Amrani, A.; Nijs, M.; Vandermeers, A.; Looze, Y.; Int. J. Bio-Chromatogr. 3, 189-206 (1997)

 show all sequences of 2.3.2.5

Data extracted from this reference:

General Stability
General Stability Organism
resistance to denaturation induced by guanidine-HCl Carica papaya
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Carica papaya
-
-
-
Source Tissue
Source Tissue Commentary Organism Textmining
latex
-
 Carica papaya
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-glutamine tert-butyl ester
-
 487999 Carica papaya L-pyroglutamate tert-butyl ester + NH3
-
-
-
 ?
More Cys residues do not participate to the catalytic events 487999 Carica papaya ?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
33
-
 L-glutamine tert-butyl ester
-
 Carica papaya
General Stability (protein specific)
General Stability Organism
resistance to denaturation induced by guanidine-HCl Carica papaya
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
latex
-
 Carica papaya
-
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-glutamine tert-butyl ester
-
 487999 Carica papaya L-pyroglutamate tert-butyl ester + NH3
-
-
-
 ?
More Cys residues do not participate to the catalytic events 487999 Carica papaya ?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
33
-
 L-glutamine tert-butyl ester
-
 Carica papaya

See also following references to EC number 2.3.2.5 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
718944KochCrystal structures of glutamin ...Drosophila melanogasterBiochemistry517383-73922012--112-6-21-14-6--------26-----2---6----2-22--126-22-14--------26-----2-------5152
719267KochProbing secondary glutaminyl c ...Homo sapiens, Mus musculusChem. Biol. Drug Des.80937-9462012--1-17-8------2--------10---------2----1--17--82-------------10---------------
718521Hartlage-RuebsamenGlutaminyl cyclase contributes ...Homo sapiens, Mus musculusActa Neuropathol.121705-7192011-2-----------6-----5--2-------------2------------------5--2----------22---
718904Ruiz-CarrilloStructures of glycosylated mam ...Homo sapiens, Mus musculusBiochemistry506280-62882011--123-14--2---2-22-----14--------------1-23--14---2----22----14---------------
719915HuangStructures of human Golgi-resi ...Homo sapiensJ. Biol. Chem.28612439-124492011--111-4-21---5--1-----2---------8----2-21--88-22-----2----2---------------
719025CarrilloKinetic and structural charact ...Myxococcus xanthus, Zymomonas mobilisBiol. Chem.3911419-14282010--222--32-----2--2-----342---322--------2-22----32-------2----342---322-------3232
720244HuangCrystal structure and function ...Xanthomonas campestris, Xanthomonas campestris ATCC33913J. Mol. Biol.401374-3882010--2211224-2---5--2-----2---24--1-4----2-2112-244-2-----2----2---24--1-----44
695532CornellPapaya latex enzymes capable o ...Carica papayaAmino Acids38155-1652009-1--------1--5--1--1------1-1-------1-----------1----1-2------1-1---------
702268SeifertGlutaminyl cyclases display si ...Carica papaya, Homo sapiens, Mus musculus, Solanum tuberosumBiochemistry4811831-118332009--3---42-2---4--4-----13----62---8----3-----482-2-----4----13----62-------1818
703668Stephan-Mammalian glutaminyl cyclases ...Homo sapiens, Mus musculusFEBS J.2766522-65362009--2-4-13201----2--2-----20----202---13----2--4--1313201------2----20----202-------2020
704085Hartlage-RuebsamenDevelopmental expression and s ...Mus musculusInt. J. Dev. Neurosci.27825-8352009--------31---4-----10--------------------------31-------10------------------
705052BuchholzInhibitors for human glutaminy ...Homo sapiensJ. Med. Chem.527069-70802009--1---45------3--1-----2---------45----1-----4545--------1----2---------------
684997HuangA conserved hydrogen-bond netw ...Homo sapiensBiochem. J.411181-1902008---19--10-----3--1-----2----9-----------19----10-------1----2----9----------
685267CynisAmyloidogenic processing of am ...Homo sapiensBiochemistry477405-74132008--1---1------2--------1--------------1-----1--------------1---------------
686164GontsarovaGlutaminyl cyclase activity is ...Homo sapiensClin. Chim. Acta389152-1592008------2------4-----3--1--------------------2-----------3--1---------------
688416CynisIsolation of an isoenzyme of h ...Homo sapiensJ. Mol. Biol.379966-9802008--1---1061----4-----1--6----6----8----1-----10861--------1--6----6----------
688518SchillingInhibition of glutaminyl cycla ...Rattus norvegicusJ. Neurochem.1061225-12362008--1---7------3-----2--8---------7----1-----77----------2--8---------------
689131SchillingGlutaminyl cyclase inhibition ...Homo sapiensNat. Med.141106-11112008-1-----------5-----1----------------1------------------1------------------
689961CalvaresiComputational evidence for the ...Homo sapiensProteins73527-5382008-------------2---1--------------------------------------------------------
702504HookAlternative pathways for produ ...Rattus norvegicusBiol. Chem.389993-10062008-------------3-----1--1--------------------------------1--1---------------
672479SchillingIsolation and characterization ...Arabidopsis thaliana, Solanum tuberosumBiol. Chem.388145-1532007--2---462-----3-22-----642---62---------2-----4-62------22----642---62----------
685085SchillingIsolation and characterization ...Drosophila melanogasterBiochemistry4610921-109302007--1---1123-1---7--------29----23----34----2-----213423-1----------29----23----------
672393CynisInhibition of glutaminyl cycla ...Homo sapiens, Mus musculusBiochim. Biophys. Acta17641618-16252006------2----2-2-----7--8--------------------2-----2-----7--8---------------
672475GuevaraPapaya glutamine cyclotransfer ...Carica papayaBiol. Chem.3871479-14862006---1---------4---1---------------------1----------------------------------
675310BuchholzThe first potent inhibitors fo ...Homo sapiensJ. Med. Chem.49664-6772006------22------2--------1---------22----------2222-------------1---------------
675374WintjensCrystal structure of papaya gl ...Carica papayaJ. Mol. Biol.357457-4702006---1---------3-------------------------1----------------------------------
677220PawlakSnake venom glutaminyl cyclase ...Boiga dendrophila, Boiga irregularisToxicon48278-2862006-----------2-6-4---5--2--------------------------2--4--5--2---------------
657486AzarkanCrystallization and preliminar ...Carica papayaActa Crystallogr. Sect. FF6159-612005---1---------3--1--2-------------------1-------------1-2------------------
660382HuangCrystal structures of human gl ...Homo sapiensProc. Natl. Acad. Sci. USA10213117-131222005---112-313-1---3-----1--1----14-----------112--3-13-1-------1--1----14----------
660450HuangCloning, expression, character ...Homo sapiensProtein Expr. Purif.4365-722005--11--82-11--4--1--2--3----2---------1-1---8-2-11----1-2--3----2----------
671982SchillingIsolation, catalytic propertie ...Mus musculusBiochemistry4413415-134242005--1---1611-1---6-11--11-11----11--1-10----1-----161011-1----11-11-11----11--1-------
658350BoothHuman glutaminyl cyclase and b ...Homo sapiensBMC Biol.222004---------1---3-----1---------------------------1-------1------------------
658736SchillingGlutaminyl cyclases unfold glu ...Carica papaya, Homo sapiensFEBS Lett.563191-1962004------25-----5--------6----52--------------2-5------------6----52---------
658233SchillingSubstrate specificity of gluta ...Carica papaya, Homo sapiensBiol. Chem.3841583-15922003------285-2-2-2--------71----852-10-4----------2485-2-2--------71----852-10-------
659288SchillingIdentification of human glutam ...Homo sapiensJ. Biol. Chem.27849773-497792003------33--3---3--------2---------30----------3330--3----------2---------------
660424BoothHuman pituitary glutaminyl cyc ...Bos taurus, Homo sapiensProtein Expr. Purif.32141-1462003--1-1--3-----4-21--4--31---3---------1--1----3------21-4--31---3----------
487998SchillingHeterologous expression and ch ...Homo sapiensBiochemistry4110849-108572002--1----7---1-6-11---1-81---7---------1-------7---1--11--1-81---7----------
488000AzarkanEvidence that thermodynamic st ...Carica papayaBiopolymers65325-3352002-----1-------1-1---1---------------------1----------1--1------------------
487997BatemanEvidence for essential histidi ...Homo sapiensBiochemistry4011246-112502001----4-13-----3-----2-------3------------4--1-3---------2-------3----------
487994DahlCarica papaya glutamine cyclot ...Carica papayaProtein Expr. Purif.2027-362000--1----------4-----------------------1------------------------------------
487992SykesEvidence for tissue-specific f ...Bos taurusFEBS Lett.455159-1611999--------1-1--3--1--3-17-----22----------------1-1----1-3-17-----22--------
487991ObergPapaya glutamine cyclase, a pl ...Carica papayaEur. J. Biochem.258214-2221998-----1-------1--1--2--11-----------------1-----------1-2--11--------------
487999Zerhouni-Purification and characterizat ...Carica papayaInt. J. Bio-Chromatogr.3189-2061997-----1-------1-----1--2----1-------------1-------------1--2----1----------
487995GololobovSubstrate and inhibitor specif ...Carica papayaBiol. Chem. Hoppe-Seyler377395-3981996-------9-----2-----2--10----------------------9---------2--10---------------
487993GololobovSteady-state kinetics of gluta ...Carica papayaArch. Biochem. Biophys.309300-3071994----1--101----2-11--1--21--1--1----------1----101-----11-1--21--1--1--------
487996SongMolecular cloning, sequence an ...Homo sapiensJ. Mol. Endocrinol.1377-861994--1----5-----4-----21-51---5---------1-------5---------21-51---5----------
487989BusbyAn enzyme(s) that converts glu ...Bos taurus, Homo sapiens, Rattus norvegicus, Sus scrofaJ. Biol. Chem.2628532-853619871-----812-14-4-4---8--8-----1------1-------8-12-14--4--8--8-----1---------
487990FischerIdentification of a mammalian ...Bos taurus, Rattus norvegicusProc. Natl. Acad. Sci. USA843628-36321987------131--2-4-----4--8-----1--------------1-31--2-----4--8-----1---------