Crystal structure and substrate specificity of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus

Qiu, X.; Choudhry, A.E.; Janson, C.A.; Grooms, M.; Daines, R.A.; Lonsdale, J.T.; Khandekar, S.S.; Protein Sci. 14, 2087-2094 (2005)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
expression of N-terminally His2-tagged enzyme in Escherichia coli	Staphylococcus aureus

Crystallization (Commentary)

Crystallization	Organism
15 mg/ml purified enzyme in 20 mM Tris, pH 8.0, 3 mM DTT, 100 mM NaCl, 10% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.013	0.014	isovaleryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.013	0.021	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.019	-	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.02	0.022	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.024	0.037	isobutyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]	Staphylococcus aureus	-	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Commentary	Organism
recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration	Staphylococcus aureus

Reaction

Reaction	Commentary	Organism
acetyl-CoA + malonyl-[acyl-carrier protein] = acetoacetyl-[acyl-carrier protein] + CoA + CO2	ping pong reaction mechanism, catalytically important residues are Phe298 and His238	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
1e-06	-	purified recombinant enzyme, primer substrate acetyl-CoA	Staphylococcus aureus
3e-06	-	purified recombinant enzyme, primer substrate lauroyl-CoA	Staphylococcus aureus
5e-06	-	purified recombinant enzyme, primer substrate palmitoyl-CoA	Staphylococcus aureus
6e-06	-	purified recombinant enzyme, primer substrate 2-methylcrotonyl-CoA	Staphylococcus aureus
1.3e-05	-	purified recombinant enzyme, primer substrate propionyl-CoA	Staphylococcus aureus
1.5e-05	-	purified recombinant enzyme, primer substrate isovaleryl-CoA	Staphylococcus aureus
2.2e-05	-	purified recombinant enzyme, primer substrate butyryl-CoA	Staphylococcus aureus
2.4e-05	-	purified recombinant enzyme, primer substrate hexanoyl-CoA	Staphylococcus aureus
3.1e-05	-	purified recombinant enzyme, primer substrate isobutyryl-CoA	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
2-methylcrotonyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	(4E)-4-methylhex-4-enoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
acetyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
acetyl-CoA + malonyl-[acyl-carrier protein]	low activity	660466	Staphylococcus aureus	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
butyryl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
hexanoyl-CoA + malonyl-[acyl-carrier protein]	high activity	660466	Staphylococcus aureus	3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
isobutyryl-CoA + malonyl-[acyl-carrier protein]	isobutyryl-CoA is the preferred primer substrate	660466	Staphylococcus aureus	3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
isovaleryl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
lauroyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
More	the enzyme possesses a large primer binding pocket determining the substrate specificity	660466	Staphylococcus aureus	?	-	-	-	-
palmitoyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
propionyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2	-	-	-	?

Subunits

Subunits	Commentary	Organism
dimer	2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration	Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
30	-	assay at	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
3.13	-	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
3.8	-	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
4.4	7.2	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
8.1	-	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
15.5	30.5	isovaleryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
20.9	41.6	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
25.5	41.7	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
28	47.1	isobutyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7	-	assay at	Staphylococcus aureus

Cloned(Commentary) (protein specific)

Commentary	Organism
expression of N-terminally His2-tagged enzyme in Escherichia coli	Staphylococcus aureus

Crystallization (Commentary) (protein specific)

Crystallization	Organism
15 mg/ml purified enzyme in 20 mM Tris, pH 8.0, 3 mM DTT, 100 mM NaCl, 10% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution	Staphylococcus aureus

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.013	0.014	isovaleryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.013	0.021	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.019	-	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.02	0.022	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
0.024	0.037	isobutyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
acetyl-CoA + malonyl-[acyl-carrier protein]	Staphylococcus aureus	-	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	?

Purification (Commentary) (protein specific)

Commentary	Organism
recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration	Staphylococcus aureus

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
1e-06	-	purified recombinant enzyme, primer substrate acetyl-CoA	Staphylococcus aureus
3e-06	-	purified recombinant enzyme, primer substrate lauroyl-CoA	Staphylococcus aureus
5e-06	-	purified recombinant enzyme, primer substrate palmitoyl-CoA	Staphylococcus aureus
6e-06	-	purified recombinant enzyme, primer substrate 2-methylcrotonyl-CoA	Staphylococcus aureus
1.3e-05	-	purified recombinant enzyme, primer substrate propionyl-CoA	Staphylococcus aureus
1.5e-05	-	purified recombinant enzyme, primer substrate isovaleryl-CoA	Staphylococcus aureus
2.2e-05	-	purified recombinant enzyme, primer substrate butyryl-CoA	Staphylococcus aureus
2.4e-05	-	purified recombinant enzyme, primer substrate hexanoyl-CoA	Staphylococcus aureus
3.1e-05	-	purified recombinant enzyme, primer substrate isobutyryl-CoA	Staphylococcus aureus

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
2-methylcrotonyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	(4E)-4-methylhex-4-enoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
acetyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
acetyl-CoA + malonyl-[acyl-carrier protein]	low activity	660466	Staphylococcus aureus	acetoacetyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
butyryl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
hexanoyl-CoA + malonyl-[acyl-carrier protein]	high activity	660466	Staphylococcus aureus	3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
isobutyryl-CoA + malonyl-[acyl-carrier protein]	isobutyryl-CoA is the preferred primer substrate	660466	Staphylococcus aureus	3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
isovaleryl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
lauroyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
More	the enzyme possesses a large primer binding pocket determining the substrate specificity	660466	Staphylococcus aureus	?	-	-	-	-
palmitoyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2	-	-	-	?
propionyl-CoA + malonyl-[acyl-carrier protein]	-	660466	Staphylococcus aureus	3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2	-	-	-	?

Subunits (protein specific)

Subunits	Commentary	Organism
dimer	2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration	Staphylococcus aureus

Temperature Optimum [°C] (protein specific)

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
30	-	assay at	Staphylococcus aureus

Turnover Number [1/s] (protein specific)

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
3.13	-	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
3.8	-	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
4.4	7.2	acetyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
8.1	-	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
15.5	30.5	isovaleryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
20.9	41.6	Butyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
25.5	41.7	Hexanoyl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus
28	47.1	isobutyryl-CoA	pH 7.0, 30°C, recombinant enzyme	Staphylococcus aureus

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7	-	assay at	Staphylococcus aureus

See also following references to EC number 2.3.1.180 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
701521	Huynh		Cloning, expression, crystalli ...	Xanthomonas oryzae pv. oryzae	Acta Crystallogr. Sect. F	65	460-462	2009	-	-	1	1	-	-	-	-	-	-	-	-	-	5	-	-	1	-	-	-	-	-	1	2	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	2	-	-	-	-	-	-	-	-	-	1	1	-	-	-
702531	Matsumoto		Production of short-chain-leng ...	Arabidopsis thaliana, Escherichia coli	Biomacromolecules	10	686-690	2009	-	-	1	-	2	-	-	-	2	-	-	-	-	7	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	2	-	-	-	-	-	2	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
702601	Lv		Design, synthesis and biologic ...	Escherichia coli	Bioorg. Med. Chem. Lett.	19	6750-6754	2009	-	1	-	-	-	-	28	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	8	-	1	-	-	-	-	-	8	28	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
702634	Lee		Novel E. coli beta-ketoacyl-ac ...	Escherichia coli K-12	Bioorg. Med. Chem.	17	1506-1513	2009	-	-	1	-	-	-	4	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	1	-	-	-
702656	Lee		Antimicrobial natural products ...	Escherichia coli K-12	Bioorg. Med. Chem.	17	5408-5413	2009	-	-	1	-	-	-	3	-	-	-	-	1	-	2	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	3	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
702661	Li		Synthesis of C(7) modified chr ...	Escherichia coli	Bioorg. Med. Chem.	17	6264-6269	2009	-	1	1	-	-	-	16	-	-	-	-	2	-	3	-	-	1	-	-	-	-	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	1	1	-	-	-	-	-	16	-	-	-	-	-	2	-	-	-	1	-	-	-	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
703762	Singh		FabH selectivity for anteiso b ...	Listeria monocytogenes	FEMS Microbiol. Lett.	301	188-192	2009	-	-	-	-	-	-	-	7	-	-	-	6	-	4	-	-	-	-	-	-	-	-	8	-	1	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	7	-	-	-	6	-	-	-	-	-	-	-	-	8	-	1	-	-	7	-	-	-	-	-	1	1	-	7	7
704622	Veyron-Churlet		The Mycobacterium tuberculosis ...	Mycobacterium tuberculosis	J. Biol. Chem.	284	6414-6424	2009	-	-	1	1	2	-	-	-	-	-	-	2	-	3	-	1	1	-	-	-	-	-	3	-	1	-	-	-	2	-	-	-	-	-	-	-	-	1	-	1	2	-	-	-	-	-	-	-	-	2	-	-	1	1	-	-	-	-	3	-	1	-	-	-	2	-	-	-	-	1	1	-	-	-
705055	Lee		Targeting the fatty acid biosy ...	Plasmodium falciparum	J. Med. Chem.	52	952-963	2009	-	1	-	-	-	-	43	-	-	-	-	1	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	43	-	1	-	-	-	-	-	43	43	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
706427	Al-Balas		Identification of 2-aminothiaz ...	Mycobacterium tuberculosis	PLoS ONE	4	e5617	2009	-	1	-	-	-	-	11	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	6	-	1	-	-	-	-	-	6	11	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
706592	Wen		Functional expression of Franc ...	Francisella tularensis subsp. holarctica	Protein Expr. Purif.	65	83-91	2009	-	-	1	-	-	-	13	-	-	-	-	1	-	1	-	-	1	-	-	-	-	-	1	-	1	-	-	-	1	-	-	-	-	-	15	-	-	1	-	-	-	-	15	13	-	-	-	-	-	1	-	-	-	1	-	-	-	-	1	-	1	-	-	-	1	-	-	-	-	1	1	-	-	-
685507	Sachdeva		Probing reactivity and substra ...	Mycobacterium tuberculosis	Bioorg. Chem.	36	85-90	2008	-	-	-	1	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
686049	Sachdeva		Separate entrance and exit por ...	Mycobacterium tuberculosis	Chem. Biol.	15	402-412	2008	-	-	-	1	-	-	-	-	-	-	-	-	-	3	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
688834	Sachdeva		Mycobacterium tuberculosis bet ...	Mycobacterium tuberculosis	Methods Mol. Med.	142	205-213	2008	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
688880	Castillo		Bacterial beta-ketoacyl-acyl c ...	Enterococcus faecium, Escherichia coli, Haemophilus influenzae, Mycobacterium tuberculosis, Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes	Mini Rev. Med. Chem.	8	36-45	2008	-	-	-	-	-	-	33	-	-	-	-	-	-	11	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	23	-	-	-	-	-	-	-	23	33	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
690169	Li		Molecular cloning and expressi ...	Jatropha curcas	Tree Physiol.	28	921-927	2008	-	-	1	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
703548	Singh		QSAR studies on benzoylaminobe ...	Enterococcus faecalis, Escherichia coli, Neisseria meningitidis, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes	Eur. J. Med. Chem.	43	1071-1080	2008	-	-	-	-	-	-	276	-	-	-	-	6	-	13	-	-	-	-	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	276	-	-	-	-	-	6	-	-	-	-	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
672628	Alhamadsheh		Synthesis and biological evalu ...	Escherichia coli, Mycobacterium tuberculosis, Plasmodium falciparum	Bioorg. Med. Chem. Lett.	17	879-883	2007	-	-	-	-	-	-	46	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	47	-	-	-	-	-	-	-	47	46	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
676903	Wang		Discovery of platencin, a dual ...	Staphylococcus aureus	Proc. Natl. Acad. Sci. USA	104	7612-7616	2007	-	-	-	-	-	-	2	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
685898	Jeong	-	Homology modeling and docking ...	Enterococcus faecalis	Bull. Korean Chem. Soc.	28	1335-1340	2007	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
686047	Alhamadsheh		Alkyl-CoA disulfides as inhibi ...	Escherichia coli, Mycobacterium tuberculosis	Chem. Biol.	14	513-524	2007	-	-	-	1	-	-	5	-	-	-	-	-	-	5	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	1	-	-	3	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
688033	Ashek		HQSAR study of beta-ketoacyl-a ...	Enterococcus faecalis	J. Enzyme Inhib. Med. Chem.	22	7-14	2007	-	-	-	-	-	-	36	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	36	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
659493	Brown		Probing the mechanism of the M ...	Mycobacterium tuberculosis	J. Biol. Chem.	280	32539-32547	2005	-	-	1	1	9	-	-	-	-	-	-	1	-	4	-	-	1	1	-	-	-	-	3	-	1	-	-	-	2	-	-	-	-	-	-	-	-	1	-	1	9	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	3	-	1	-	-	-	2	-	-	-	-	-	-	-	-	-
659724	Musayev		Crystal structure of a substra ...	Mycobacterium tuberculosis	J. Mol. Biol.	346	1313-1321	2005	-	-	-	1	-	-	-	-	-	-	-	1	-	3	-	-	-	1	-	-	-	-	3	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	3	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
660466	Qiu		Crystal structure and substrat ...	Staphylococcus aureus	Protein Sci.	14	2087-2094	2005	-	-	1	1	-	-	-	5	-	-	-	1	-	5	-	-	1	1	-	-	9	-	11	1	1	-	-	8	1	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	5	-	-	-	1	-	-	-	1	-	-	9	-	11	1	1	-	-	8	1	-	-	-	-	-	-	-	-	-
665387	Li		Alteration of the fatty acid p ...	Streptomyces coelicolor	J. Bacteriol.	187	3795-3799	2005	-	-	-	-	1	-	-	-	-	-	-	2	-	6	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
665975	Nie		Structure-based design, synthe ...	Enterococcus faecalis	J. Med. Chem.	48	1596-1609	2005	-	1	-	1	-	-	59	-	-	-	-	1	-	2	-	-	-	-	-	-	-	-	2	-	-	-	-	-	1	-	-	-	-	-	46	-	1	-	-	1	-	-	46	59	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
663647	He		1,2-dithiole-3-ones as potent ...	Escherichia coli, Staphylococcus aureus	Antimicrob. Agents Chemother.	48	3093-3102	2004	-	-	-	-	-	-	8	-	-	-	-	2	-	4	-	-	-	-	-	-	-	-	4	-	2	-	-	-	2	-	-	-	-	-	2	-	-	-	-	-	-	-	2	8	-	-	-	-	-	2	-	-	-	-	-	-	-	-	4	-	2	-	-	-	2	-	-	-	-	-	-	-	-	-
663702	Nomura		Coexpression of genetically en ...	Escherichia coli	Appl. Environ. Microbiol.	70	999-1007	2004	-	-	1	-	19	-	-	-	-	-	-	1	-	4	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	19	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
659294	Lai		beta-Ketoacyl-acyl carrier pro ...	Escherichia coli, Lactococcus lactis ssp. lactis	J. Biol. Chem.	278	51494-51503	2003	-	-	-	-	2	-	-	-	1	-	-	3	-	6	-	-	-	-	-	-	1	-	4	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	1	-	-	3	-	-	-	-	-	-	1	-	4	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
666668	Jones		beta-Ketoacyl-acyl carrier pro ...	Pisum sativum	Planta	216	752-761	2003	-	-	1	-	-	-	1	2	-	-	1	1	-	6	-	-	1	1	-	1	-	-	2	1	1	-	-	-	1	-	-	-	-	-	1	-	-	1	-	-	-	-	1	1	-	2	-	-	1	1	-	-	-	1	-	1	-	-	2	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
486946	He		Purification, characterization ...	Staphylococcus aureus	Antimicrob. Agents Chemother.	46	1310-1318	2002	-	2	1	-	-	-	5	3	-	-	1	1	-	6	-	1	1	-	-	-	-	-	4	1	1	-	-	3	1	-	-	-	-	-	3	-	2	1	-	-	-	-	3	5	-	3	-	-	1	1	-	-	1	1	-	-	-	-	4	1	1	-	-	3	1	-	-	-	-	-	-	-	-	-
664003	Florova		Enzymes involved in fatty acid ...	Streptomyces glaucescens	Biochemistry	41	10462-10471	2002	-	-	-	-	-	-	-	-	-	-	-	2	-	1	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
486938	Smirnova		Engineered fatty acid biosynth ...	Streptomyces glaucescens	J. Bacteriol.	183	2335-2342	2001	-	-	1	-	3	-	-	-	-	-	-	1	-	5	-	-	1	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	3	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
486939	Khandekar		Identification, substrate spec ...	Streptococcus pneumoniae	J. Biol. Chem.	276	30024-30030	2001	-	1	1	-	-	-	3	2	-	-	-	1	-	6	-	-	1	1	-	-	2	-	6	1	-	-	-	-	1	-	-	-	-	-	3	-	1	1	-	-	-	-	3	3	-	2	-	-	-	1	-	-	-	1	-	-	2	-	6	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-
663684	Kiatpapan		Molecular characterization of ...	Lactobacillus plantarum	Appl. Environ. Microbiol.	67	426-433	2001	-	-	1	-	-	-	-	-	-	-	-	1	-	7	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
665365	Revill		beta-Ketoacyl acyl carrier pro ...	Streptomyces coelicolor	J. Bacteriol.	183	3526-3530	2001	1	-	1	-	-	-	-	-	-	-	-	2	-	4	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
665477	Scarsdale		Crystal structure of the Mycob ...	Mycobacterium tuberculosis	J. Biol. Chem.	276	20516-20522	2001	-	1	-	1	-	-	-	-	-	-	-	1	-	4	-	-	1	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
666011	Qiu		Refined structures of beta-ket ...	Escherichia coli	J. Mol. Biol.	307	341-356	2001	-	-	-	1	-	-	-	-	-	-	-	1	-	2	-	-	-	1	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
666615	Dehesh		Overexpression of 3-ketoacyl-a ...	Brassica napus, Cuphea hookeriana, Nicotiana tabacum, Spinacia oleracea	Plant Physiol.	125	1103-1114	2001	-	-	3	-	3	-	-	-	-	-	-	7	-	10	-	-	-	-	-	9	3	-	7	1	4	-	-	-	4	-	-	-	-	2	-	-	-	3	-	-	3	-	-	-	-	-	-	-	-	7	-	-	-	-	-	9	3	-	7	1	4	-	-	-	4	-	-	2	-	-	-	-	-	-
486937	Choi		beta-ketoacyl-acyl carrier pro ...	Bacillus subtilis	J. Bacteriol.	182	365-370	2000	-	-	1	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	5	-	11	-	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	5	-	11	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
663471	Janson		Crystallization of Escherichia ...	Escherichia coli	Acta Crystallogr. Sect. D	56	747-748	2000	-	1	-	1	-	-	-	-	-	-	-	1	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
663822	Khandekar		Expression, purification, and ...	Escherichia coli	Biochem. Biophys. Res. Commun.	270	100-107	2000	-	-	1	1	-	-	-	-	-	-	1	1	-	3	-	-	1	-	-	-	1	-	2	2	1	-	2	-	1	-	-	-	-	1	-	-	-	1	-	1	-	-	-	-	-	-	-	-	1	1	-	-	-	1	-	-	1	-	2	2	1	-	2	-	1	-	-	1	-	-	-	-	-	-
663922	Abbadi		Reaction mechanism of recombin ...	Cuphea wrightii	Biochem. J.	345	153-160	2000	-	-	1	-	9	-	-	3	-	-	-	1	-	5	-	-	1	1	-	1	5	-	1	-	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	9	-	-	-	-	3	-	-	-	1	-	-	-	1	-	1	5	-	1	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
665474	Choi		Identification and substrate s ...	Mycobacterium tuberculosis	J. Biol. Chem.	275	28201-28207	2000	-	-	1	-	-	-	7	-	-	-	-	1	-	6	-	-	1	1	-	-	2	-	9	1	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	7	-	-	-	-	-	1	-	-	-	1	-	-	2	-	9	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
666354	Hwang		Molecular cloning and function ...	Perilla frutescens	Mol. Cells	10	375-381	2000	-	-	1	-	-	-	-	-	-	-	-	1	-	5	-	-	-	-	-	2	-	-	1	-	1	-	-	-	1	-	-	-	-	2	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	4	-	-	2	-	2	-	-	-	2	-	-	2	-	-	-	-	-	-
666596	Abbadi		Knockout of the regulatory sit ...	Cuphea wrightii	Plant J.	24	1-9	2000	-	-	1	-	3	-	1	-	-	-	-	2	-	7	-	-	1	-	-	-	1	-	3	-	1	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	6	-	-	2	-	-	-	-	-	2	-	-	-	2	-	-	2	-	4	-	2	-	-	-	2	-	-	-	-	-	-	-	-	-
666950	Davies		The 1.8 A crystal structure an ...	Escherichia coli	Structure	8	185-195	2000	-	-	1	1	3	-	1	-	-	-	-	1	-	3	-	-	1	1	-	-	-	-	3	2	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	1	3	-	-	1	-	-	-	-	-	1	-	-	-	1	-	-	-	-	3	2	1	-	-	-	1	-	-	-	-	-	-	-	-	-
486934	Bao		Purification and properties of ...	Streptomyces peucetius	Biochemistry	38	9752-9757	1999	-	-	1	-	-	-	-	-	-	-	1	1	-	4	-	-	1	-	-	-	-	-	3	1	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-	1	-	-	-	-	3	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
665472	Qiu		Crystal structure of beta-keto ...	Escherichia coli	J. Biol. Chem.	274	36465-36471	1999	-	1	-	1	-	-	-	-	-	-	-	1	-	2	-	-	-	1	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
486933	Han		Characterization of beta-ketoa ...	Streptomyces glaucescens	J. Bacteriol.	180	4481-4486	1998	-	1	1	-	-	-	1	4	-	-	1	4	-	5	-	-	1	-	-	-	-	-	5	1	1	-	-	-	1	-	-	-	-	-	-	-	1	1	-	-	-	-	-	1	-	4	-	-	1	4	-	-	-	1	-	-	-	-	5	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
665468	Heath		Inhibition of beta-ketoacyl-ac ...	Escherichia coli	J. Biol. Chem.	271	10996-11000	1996	-	-	1	-	-	-	2	2	-	-	-	1	-	2	-	-	1	-	-	-	1	-	5	-	1	-	-	-	1	-	-	-	1	-	-	-	-	1	-	-	-	-	-	2	1	2	-	-	-	1	-	-	-	1	-	-	1	-	5	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
666666	Brueck	-	In-vitro evidence for feed-bac ...	Cuphea lanceolata	Planta	198	271-278	1996	-	-	-	-	-	-	5	1	-	-	-	1	-	1	-	-	-	-	-	1	-	-	2	-	1	-	-	-	-	-	-	-	1	-	2	-	-	-	-	-	-	-	2	5	1	1	-	-	-	1	-	-	-	-	-	1	-	-	2	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-
486932	Verwoert		Modification of Brassica napus ...	Escherichia coli	Plant Mol. Biol.	27	875-886	1995	-	-	1	-	1	-	-	2	-	-	-	-	-	4	-	-	-	-	-	2	1	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	2	1	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
486931	Clough		Purification and characterizat ...	Spinacia oleracea	J. Biol. Chem.	267	20992-20998	1992	-	-	-	-	-	1	2	-	-	-	1	1	-	3	-	-	1	-	-	1	1	1	2	1	1	-	-	-	1	-	-	1	-	-	-	-	-	-	1	-	-	1	-	2	-	-	-	-	1	1	-	-	-	1	-	1	1	1	2	1	1	-	-	-	1	-	-	-	-	-	-	-	-	-
665464	Tsay		Isolation and characterization ...	Escherichia coli K-12	J. Biol. Chem.	267	6807-6814	1992	-	-	1	-	-	-	-	-	-	-	-	1	-	2	-	-	1	1	-	-	2	-	2	1	1	-	-	-	1	-	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	2	-	2	1	1	-	-	-	1	-	-	1	-	-	-	-	-	-