Serine hydroxymethyltransferase from spinach leaf mitochondria. Purification and characterization

Henricson, D.; Ericson, I.; Physiol. Plant. 74, 602-606 (1988)

 show all sequences of 2.1.2.1

Data extracted from this reference:

General Stability
General Stability Organism
folate stabilizes Spinacia oleracea
Inhibitors
Inhibitors Commentary Organism Structure
carboxymethoxylamine strong Spinacia oleracea
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.15 0.9 L-serine
-
 Spinacia oleracea
Localization
Localization Commentary Organism GeneOntology No. Textmining
mitochondrion
-
 Spinacia oleracea 5739
-
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
209000
-
 gel filtration Spinacia oleracea
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Spinacia oleracea
-
-
-
Purification (Commentary)
Commentary Organism
-
 Spinacia oleracea
Source Tissue
Source Tissue Commentary Organism Textmining
leaf
-
 Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
7.8
-
-
 Spinacia oleracea
Storage Stability
Storage Stability Organism
4°C, 8 weeks in the presence of folate Spinacia oleracea
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-serine + tetrahydrofolate
-
 441391 Spinacia oleracea glycine + 5,10-methylenetetrahydrofolate + H2O
-
-
-
 ?
Subunits
Subunits Commentary Organism
homotetramer 4 * 53000, SDS-PAGE Spinacia oleracea
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Spinacia oleracea
Ki Value [mM]
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.014
-
 carboxymethoxylamine
-
 Spinacia oleracea
General Stability (protein specific)
General Stability Organism
folate stabilizes Spinacia oleracea
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
carboxymethoxylamine strong Spinacia oleracea
Ki Value [mM] (protein specific)
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.014
-
 carboxymethoxylamine
-
 Spinacia oleracea
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.15 0.9 L-serine
-
 Spinacia oleracea
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
mitochondrion
-
 Spinacia oleracea 5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
209000
-
 gel filtration Spinacia oleracea
Purification (Commentary) (protein specific)
Commentary Organism
-
 Spinacia oleracea
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
leaf
-
 Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
7.8
-
-
 Spinacia oleracea
Storage Stability (protein specific)
Storage Stability Organism
4°C, 8 weeks in the presence of folate Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-serine + tetrahydrofolate
-
 441391 Spinacia oleracea glycine + 5,10-methylenetetrahydrofolate + H2O
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
homotetramer 4 * 53000, SDS-PAGE Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.5
-
-
 Spinacia oleracea

See also following references to EC number 2.1.2.1 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
704060Siglioccolo-Structural adaptation of serin ...Escherichia coli, Geobacillus stearothermophilus, Homo sapiens, Mus musculus, Oryctolagus cuniculusInt. J. Biol. Macromol.4637-462010-------------5------------------------------------------------------------
700740JamaiArabidopsis photorespiratory s ...Arabidopsis thalianaPlant Cell21595-6062009--------2----3-----1--1-----------------------2--------1--1---------1111--
702126PaiStructural and functional stud ...Geobacillus stearothermophilusBiochem. J.418635-6422009--113--2-----3--1-----21--11---1-----1113----2-------1----21--11----------
703634FlorioThe role of evolutionarily con ...Escherichia coliFEBS J.276132-1432009--1-3--7--1--3--------12---3---1-----11-3----7--1---------12---3----------
703653SopitthummakhunSerine hydroxymethyltransferas ...Plasmodium vivaxFEBS J.2764023-40362009--1----3--1--5--1---2-31-1-311-1-----11------3--1----1--2-31-1-311--------
703674FlorioStructural stability of the co ...Escherichia coliFEBS J.2767319-73282009----3-1---1--3--1-1---1--------1------1-3--1----1----11---1---------------
704565FoxMechanism of the internal ribo ...Homo sapiensJ. Biol. Chem.28431085-310962009-----------1-2-----1--1--------------------------1-----1--1---------1111--
704566FoxA UV-responsive internal ribos ...Homo sapiensJ. Biol. Chem.28431097-311082009--------2----2-----4--1-----------------------2--------4--1---------1111--
704756SchweitzerThe serine hydroxymethyltransf ...Corynebacterium glutamicumJ. Biotechnol.139214-2212009-------------4------1-1--------1------1-----------------1-1---------1--1--
705602MaenpuenCharacterization of Plasmodium ...Plasmodium falciparumMol. Biochem. Parasitol.16863-732009-11--115--1--5--1-----21---3---1----111--1-1-5--1----1----21---3--------22
705603PangCatalytic and ligand-binding c ...Plasmodium falciparumMol. Biochem. Parasitol.16874-832009--1---92--1--3--1-----11---1---1--9--11---99-2--1----1----11---1----------
689318LeartsakulpanichCloning and characterization o ...Plasmodium vivaxParasitol. Int.57223-2282008--1-1---1----4------1--1-------------1--1-----1---------1--1--------------
703629BhavaniImportance of tyrosine residue ...Geobacillus stearothermophilusFEBS J.2754606-46192008--112--------3--1---2-1--------1-----1112------------1--2-1---------------
703863Choi-Human serine hydroxymethyltran ...Homo sapiensGenes Genomics30541-5482008--------1--1-1-----1--2--------1------1-------1--1-----1--2---------------
704347BhattCharacterization of pyridoxal ...Bacillus subtilisJ. Biochem.144295-3032008--1-------11-4--1-1---11--1----1-----11---------11---11---11--1-----------
704435MacFarlaneCytoplasmic serine hydroxymeth ...Mus musculusJ. Biol. Chem.28325846-258532008--------3----4-----2--1-----------------------3--------2--1----------33---
671499StolzReduced folate supply as a key ...Corynebacterium glutamicumAppl. Environ. Microbiol.73750-755200721--1--------3--------1------------21---1-----------------1---------------
673397Zuo-Cloning, DNA shuffling and exp ...Escherichia coliEnzyme Microb. Technol.40569-5772007--1-1--------2--1------1--1-2--------1--1------------1-----1--1-2---------
676982VatsyayanMolecular cloning and biochemi ...Leishmania donovaniProtein Expr. Purif.52433-4402007-11---33--2--5--1--21-21221-21---1--11-----3-3--2----1-21-21221-21-1------
685708Makart StefaCharacterization of the AlkS/P ...Escherichia coliBiotechnol. Bioeng.96326-3362007-11----------4------1-1-------------11------------------1-1---------------
686353ChangSerine hydroxymethyltransferas ...Danio rerioDrug Metab. Dispos.352127-21372007--1---113-1--5--1--4-2111--11--1--2--12---22-13-2----1-6-2222--12---------
673412MukherjeeIdentification and biochemical ...Trichomonas vaginalisEukaryot. Cell52072-20782006111-2--42-1--3--1-----11---4-------111--2----42-1----1----11---4----------
674993Franca-Molecular dynamics of the inte ...Homo sapiens, Plasmodium falciparumJ. Braz. Chem. Soc.171383-13922006-2----2------2----------------------2------2------------------------------
675480GriffithsLateral transfers of serine hy ...Simkania negevensis, Waddlia chondrophilaJ. Mol. Evol.63283-2962006-2-----------15----------------------2-------------------------------------
675874GagnonStage specific gene expression ...Leishmania infantum, Leishmania majorMol. Biochem. Parasitol.15063-712006-11---2-2-2--7-----2--1-------------11-----2--2-2------2--1---------------
676503SchjoerringAntisense reduction of serine ...Solanum tuberosumPlant J.4571-822006--1-----2-1--3-----1--1--------------1--------2-1------1--1---------------
676615EisenhutThe plant-like C2 glycolate cy ...Synechocystis sp.Plant Physiol.142333-3422006-11-1--------4--1-----11------------11--1------------1----11--------------
676954ChangCloning, expression, purificat ...Danio rerioProtein Expr. Purif.46212-2202006111----12-1--5--1--11111-1-1-------111-------12-1----1-11111-1-1----------
658127BhavaniRole of Lys-226 in the catalyt ...Geobacillus stearothermophilusBiochemistry446929-69372005---12--------3--1-----1--------1------112------------1----1---------------
659572LewisLow serine hydroxymethyltransf ...Homo sapiens, Ovis aries, Rattus norvegicusJ. Clin. Endocrinol. Metab.901594-15982005-----------3-7-----5--3--------------------------3-----5--3---------------
659772ScheerActivities of hepatic cytosoli ...Rattus norvegicusJ. Nutr.135233-2382005--------6--1-3-----2--1--------1------1-------6--1-----2--1---------------
660180MorenoArabidopsis SHMT1, a serine hy ...Arabidopsis thalianaPlant J.41451-4632005--1--------1-4--------1--------------1-----------1--------1---------------
662384Goyer5-Formyltetrahydrofolate is an ...Arabidopsis thalianaJ. Biol. Chem.28026137-261422005----1-1-1----3-----1--1-----------------1--1--1--------1--1---------------
671589FuProperties of human and rabbit ...Homo sapiens, Oryctolagus cuniculusArch. Biochem. Biophys.44292-10120052-2-4--64----6--2-----4----6-------2-2--4----64------2----4----6----------
674506PerryMimosine attenuates serine hyd ...Homo sapiensJ. Biol. Chem.280396-4002005-11-1-1-11---3-----2----------------11--1--1--11-------2------------------
658083SzebenyiSerine hydroxymethyltransferas ...Oryctolagus cuniculusBiochemistry436865-68762004---12--2-----2--------1----2-----------12----2------------1----2----------
660460BhattThe C-terminal domain of dimer ...Bacillus subtilis, Geobacillus stearothermophilusProtein Sci.132184-21952004----22-------4--2------2----------------22-----------2-----2--------------
657809SchlüpenDisruption of the SHM2 gene, e ...Eremothecium gossypiiBiochem. J.369263-2732003-----------1-4--------1--------------------------1--------1---------------
657811JalaIdentification of amino acid r ...Ovis ariesBiochem. J.369469-4762003----13---2-1--2-----2--11-------1------1-13-----2-1------2--11--------------
658892JalaDisruption of distal interacti ...Ovis ariesIndian J. Biochem. Biophys.40226-2372003--1-3--41-1--1-----1--11--24---------1--3----41-1------1--11--24----------
659226FuLocation of the pteroylpolyglu ...Oryctolagus cuniculusJ. Biol. Chem.2782645-26532003---1----2----3--------1----------------1------2-----------1---------------
659237FuRole of proline residues in th ...Escherichia coliJ. Biol. Chem.27831088-310942003----10--14-----3--1-----1---312------------10----14-------1----1---312----------
659265ChaturvediUnusual structural, functional ...Mycobacterium tuberculosisJ. Biol. Chem.27840793-408052003--1--4----2--4--1-----31--2---41-----22--4------2----2----42--2---4-------
659269AngelaccioCatalytic and thermodynamic pr ...Methanocaldococcus jannaschiiJ. Biol. Chem.27841789-417972003--1--1-2--1--3--1-----211--2---------1---1---2--1----1----211--2----------
659754AgrawalCloning, expression, activity ...Escherichia coli, Homo sapiensJ. Mol. Microbiol. Biotechnol.667-752003-21-----1--1-10--1-----3--------2----212-------1--1---1----3---------------
660469PaiardiniStructural plasticity of therm ...Aeropyrum pernix, Aquifex aeolicus, Archaeoglobus fulgidus, Methanocaldococcus jannaschii, Methanothermobacter marburgensis, Methanothermobacter thermautotrophicus, Pyrococcus abyssi, Pyrococcus horikoshii, Sulfolobus solfataricus, Thermotoga maritimaProteins50122-1342003-------------10--------10---10-------------------------------10---10-----------
441431TrivediCrystal structure of binary an ...Geobacillus stearothermophilusJ. Biol. Chem.27717161-171692002-111-------1-4---1----31-------1----1111---------1--------31--------------
441432JalaOverexpression and characteriz ...Geobacillus stearothermophilusJ. Biosci.27233-2422002--1----3--21-5--11--4-211-33---1-----11------3--21---1--4-211-33----------
441424ContestabileL-Threonine aldolase, serine h ...Escherichia coliEur. J. Biochem.2686508-65252001--1---1------4---1----4--------------1-----1--------------4---------------
441427Appaji RaoMolecular organization, cataly ...Escherichia coli, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Saccharomyces cerevisiaeInt. J. Biochem. Cell Biol.32405-4162000-641--36-11--6-10-5----6-2411-------6----6461---36--11--6--5---6-2411--------------
441428Talwar-Role of Pro-297 in the catalyt ...Ovis ariesBiochem. J.350849-8532000--1-1--4--1--1------2-22--12---1-----11-1----4--1-------2-22--12----------
441429TalwarA change in reaction specifici ...Ovis ariesEur. J. Biochem.267929-9342000--1-1--------4-----13-5--------1-----11-1--------------13-5---------------
441430RaoThe role of Glu74 and Tyr82 in ...Ovis ariesEur. J. Biochem.2675967-59762000-11-3--33-11-5---1-33-22-113---1----111-3----33-11-----33-22-113----------
441434OgawaSerine hydroxymethyltransferas ...Cricetulus griseus, Escherichia coli, Homo sapiens, Neurospora crassa, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Vigna radiataInt. J. Biochem. Cell Biol.32289-3012000-542--9---79-11---9--7-348-------8----5482---9----79------7-348--------------
441436LiuHuman cytoplasmic serine hydro ...Homo sapiensBiochemistry3911523-115312000--1-----3--1-3-1-1----3--------------1--------3--1--1-----3---------------
441438VatcherIdentification and characteriz ...Caenorhabditis briggsae, Caenorhabditis elegansGene230137-1441999--2----------5-----------------------2------------------------------------
441423Di SalvoPurification and characterizat ...Oryctolagus cuniculusProtein Expr. Purif.13177-1831998--1----23--2-3--1--21-3----2---------1-------23--2---1-21-3----2----------
441433LiuGene cloning, nucleotide seque ...Escherichia coliAppl. Environ. Microbiol.64549-5541998-----------1-1--------2--------------------------1--------2---------------
441422FrattePurification and properties of ...Sulfolobus solfataricusJ. Bacteriol.1797456-74611997------2----1-3--1---1-21-------1------1----2-----1---1--1-21--------------
441435ChaveIsolation and characterization ...Homo sapiensBiochem. Soc. Trans.2553S1997--1-----2--1-2--------2--------2-----12-------2--1--------2---------------
441426BrahatheeswaranInteraction of sheep liver apo ...Ovis ariesArch. Biochem. Biophys.330363-3721996----------3--3-----2------2---11------1---------3------2------2---1-------
441437Sakamoto-Purification and characterizat ...Euglena gracilisBiosci. Biotechnol. Biochem.601941-19441996------923-1--1--1---4-41----1--2------2----9-23-1----1--4-41----1---------
441421KruschwitzExpression, purification, and ...Homo sapiensProtein Expr. Purif.6411-4161995--1----22----3--1---1-1----1---1-----11------22------1--1-1----1----------
441425Jagath-ReddycDNA cloning, overexpression i ...Ovis ariesEur. J. Biochem.230533-5371995--1---123--1-3--1--24-21-------------1-----1-23--1---1-24-21--------------
441390IdePurification of serine hydroxy ...Geobacillus stearothermophilusJ. Chromatogr.596203-2091992--1----------3--1--------------------1---------------1--------------------
441409Sakamoto-Purification and characterizat ...Euglena gracilisAgric. Biol. Chem.552243-22491991------811-1--1--1---2-41----1--24-----2----8411-1----1--2-41----1---------
441417MalthouseA comparative study of the kin ...Bos taurusBiochem. J.274807-8121991-------------2---1----11-------1------1-------------------11--------------
441418Stover5-Formyltetrahydrofolate polyg ...Escherichia coli, Oryctolagus cuniculusJ. Biol. Chem.2661543-15501991------8-2----3---2----2--------21-----2----81-2-----------2---------------
441405StrongPurification and properties of ...Mus musculusJ. Biol. Chem.26512149-121551990------222-12-3--11-11-31---1----1----------2122-12---1-11-31---1----------
391984BourguignonResolution and characterizatio ...Pisum sativumBiochem. J.255169-1781988--------2-1--2--1--21-11-------1------1-------2-1----1-21-11--------------
441391Henricson-Serine hydroxymethyltransferas ...Spinacia oleraceaPhysiol. Plant.74602-6061988-----1111-1--1--1--11111----1---1--------1-1111-1----1-11111----1---------
441398MasudaAffinity purification and char ...Rattus norvegicusJ. Biochem.101643-6521987------813-11-2--1---1-61----2--2------2----8-13-11---1--1-61----2---------
441410MiyazakiPurification and characterizat ...Hyphomicrobium methylovorumEur. J. Biochem.162533-5401987------202--21-3--1---1-21222-3212------2----20-2--21---1--1-21222-321-------
441411Miyazaki-Futher characterization of ser ...Hyphomicrobium methylovorumAgric. Biol. Chem.512587-25891987------2----1-1--------4--------1------1----2-----1--------4---------------
441407MitchellSerine hydroxymethyltransferas ...Glycine maxPlant Physiol.81553-5571986-----183--11-3--11-22121---112--4--------1-843--11---1-22121---112--------
441412SchirchSerine hydroxymethyltransferas ...Escherichia coliJ. Bacteriol.1631-71985--1---43--21-3--11--1-31---2--13-----13----4-3--21---1--1-31---2--1-------
441416ManoharKinetic mechanism of the inter ...Ovis ariesBiochemistry234116-41221984------2------3---1-1--1--------1------1----2-----------1--1---------------
441393Manohar-Purification, physicochemical ...Ovis ariesJ. Biosci.431-501982------3-1-1--1--1-----1-1-1----------------3--1-1----1----1-1-1-----------
441408RaoPurification and regulatory pr ...Vigna radiataPlant Physiol.6911-181982-----6821-12-2--1--11-3121--11-12-----1--6-8221-12---1-11-3121--11--------
441420SchirchSerine hydroxymethyltransferas ...Bos taurus, Cricetulus griseus, Neurospora crassa, Oryctolagus cuniculus, Ovis aries, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar TyphimuriumAdv. Enzymol. Relat. Areas Mol. Biol.5383-1121982------22512--7-7---6-1-1375--1---110------10----22-512--7-----1-1375--1---1-------
441399OgawaPurification and characterizat ...Rattus norvegicusJ. Biochem.90381-3901981------1-5-31-3--1--11-71----1--2------2----1--5-31---1-11-71----1---------
441394SchirchPurification and properties of ...Oryctolagus cuniculusJ. Biol. Chem.2557801-78061980---1--423-1--1--1---1-11---1---1------11---4-23-1----1--1-11---1----------
441403RameshPurification and physicochemic ...Macaca radiataBiochem. J.187623-63619801----4813-11-1--11-522311-412--2---1--2--4-8-13-11---1-522311-412---------
441404RameshInhibition of monkey liver ser ...Macaca radiataBiochem. J.187249-2521980------1------1---1----1--------------------1--------------1---------------
441419AkhtarMechanistic, inhibitory and st ...Oryctolagus cuniculusBiochem. J.145159-1681975------5-5----3---1-11-1--------1------1----5--5--------11-1---------------
441413NakamuraPurification and characterizat ...Saccharomyces cerevisiaeBiochim. Biophys. Acta327328-3351973-----212-311-1--1---142---2-1--2------2--2-1-2-311---1--142---2-1---------
441415El-Obeid-Glycidaldehyde, an inhibitor d ...Oryctolagus cuniculusBiochem. Soc. Trans.11274-12761973------2-1--1-1---1----2--------1------1----2--1--1--------2---------------
441402Akhtar-Interaction of serine transhyd ...Oryctolagus cuniculusBiochim. Biophys. Acta791791-7991972------4-1----1--11--1-1--------1------1----4--1------1--1-1---------------
441401FujiokaPurification and properties of ...Oryctolagus cuniculusBiochim. Biophys. Acta185338-3491969---1--82332--1--1---312-----11-12-----11---822332----1--312-----11--------
441400SchirchSerine transhydroxymethylase. ...Oryctolagus cuniculusJ. Biol. Chem.2435651-56551968------81-----1--1---1-1--------27-----2----871-------1--1-1---------------