Purification and properties of methanol:5-hydroxybenzimidazolylcobamide methyltransferase from Methanosarcina barkeri

Van der Meijden, P.; te Brömmelstroet, B.W.; Poirot, C.M.; van der Drift, C.; Vogels, G.D.; J. Bacteriol. 160, 629-635 (1984)

 show all sequences of 2.1.1.90

Data extracted from this reference:

Activating Compound
Activating Compound Commentary Organism Structure
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri DSM800
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri DSM804
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri Fusaro
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri Fusaro, DSM 804
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri MS
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri NaT1
additional information reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process; reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri DSM800
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri DSM804
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri Fusaro
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri Fusaro, DSM 804
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri MS
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri NaT1
-
General Stability
General Stability Organism
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri Fusaro
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri NaT1
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri Fusaro, DSM 804
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri MS
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri DSM804
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri DSM800
Inhibitors
Inhibitors Commentary Organism Structure
HgCl2 1 mM, 90% inhibition Methanosarcina barkeri
Na2SO3 1 mM, 50% inhibition Methanosarcina barkeri
NaNO2 0.1 mM, 90% inhibition Methanosarcina barkeri
NH4Cl 400 mM, 93% inhibition Methanosarcina barkeri
O2 inactivation by O2 and other oxidizing agents Methanosarcina barkeri
pyridoxal 5'-phosphate 1 mM, 74% inhibition Methanosarcina barkeri
Metals/Ions
Metals/Ions Commentary Organism Structure
Ba2+ metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity Methanosarcina barkeri
Ca2+ metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity Methanosarcina barkeri
Co2+ metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity Methanosarcina barkeri
Mg2+ metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity Methanosarcina barkeri
Mn2+ metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity Methanosarcina barkeri
Ni2+ metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity Methanosarcina barkeri
Sr2+ metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity Methanosarcina barkeri
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
122000
-
 PAGE with different gel concentrations Methanosarcina barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
methanol + 5-hydroxybenzimidazolylcobamide Methanosarcina barkeri one of the enzymes responsible for the transmethylation from methanol to coenzyme M Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 Methanosarcina barkeri ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Methanosarcina barkeri
-
-
-
Oxidation Stability
Oxidation Stability Organism
oxygen-sensitive Methanosarcina barkeri
Purification (Commentary)
Commentary Organism
-
 Methanosarcina barkeri
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
-
 485660 Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 485660 Methanosarcina barkeri ?
methanol + 5-hydroxybenzimidazolylcobamide one of the enzymes responsible for the transmethylation from methanol to coenzyme M 485660 Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 485660 Methanosarcina barkeri ?
Subunits
Subunits Commentary Organism
trimer alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
Temperature Optimum [°C]
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Methanosarcina barkeri
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.2
-
 assay at Methanosarcina barkeri
Cofactor
Cofactor Commentary Organism Structure
5-Hydroxybenzimidazolylcobamide 3-4 molecules bound, acting as methyl acceptor Methanosarcina barkeri
Activating Compound (protein specific)
Activating Compound Commentary Organism Structure
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri DSM800
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri DSM804
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri Fusaro
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri Fusaro, DSM 804
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri MS
ATP half-maximal activation at 0.16 mM ATP Methanosarcina barkeri NaT1
additional information reductive activation by a mixture of H2, ferredoxin and hydrogenase but also by CO, ATP, GTP or CTP needed in the reductive activation process; reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri DSM800
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri DSM804
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri Fusaro
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri Fusaro, DSM 804
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri MS
-
additional information reductive activation by a mixture of H2, ferredoxin needed in the reductive activation process Methanosarcina barkeri NaT1
-
Cofactor (protein specific)
Cofactor Commentary Organism Structure
5-Hydroxybenzimidazolylcobamide 3-4 molecules bound, acting as methyl acceptor Methanosarcina barkeri
General Stability (protein specific)
General Stability Organism
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri Fusaro
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri NaT1
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri Fusaro, DSM 804
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri MS
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri DSM804
presence of divalent cations e.g. Mg2+, Mn2+, Sr2+, Ca2+ or Ba2+ are required for stability Methanosarcina barkeri DSM800
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
HgCl2 1 mM, 90% inhibition Methanosarcina barkeri
Na2SO3 1 mM, 50% inhibition Methanosarcina barkeri
NaNO2 0.1 mM, 90% inhibition Methanosarcina barkeri
NH4Cl 400 mM, 93% inhibition Methanosarcina barkeri
O2 inactivation by O2 and other oxidizing agents Methanosarcina barkeri
pyridoxal 5'-phosphate 1 mM, 74% inhibition Methanosarcina barkeri
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Ba2+ metal-free enzyme preparation has no activity, addition of Ba2+ restores 27% of the original activity Methanosarcina barkeri
Ca2+ metal-free enzyme preparation has no activity, addition of Ca2+ restores 35% of the original activity Methanosarcina barkeri
Co2+ metal-free enzyme preparation has no activity, addition of Co+ restores 46% of the original activity Methanosarcina barkeri
Mg2+ metal-free enzyme preparation has no activity, addition of Mg2+ restores 66% of the original activity Methanosarcina barkeri
Mn2+ metal-free enzyme preparation has no activity, addition of Mn2+ restores 27% of the original activity Methanosarcina barkeri
Ni2+ metal-free enzyme preparation has no activity, addition of Ni2+ restores 21% of the original activity Methanosarcina barkeri
Sr2+ metal-free enzyme preparation has no activity, addition of Sr2+ restores 20% of the original activity Methanosarcina barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
122000
-
 PAGE with different gel concentrations Methanosarcina barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
methanol + 5-hydroxybenzimidazolylcobamide Methanosarcina barkeri one of the enzymes responsible for the transmethylation from methanol to coenzyme M Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 Methanosarcina barkeri ?
Oxidation Stability (protein specific)
Oxidation Stability Organism
oxygen-sensitive Methanosarcina barkeri
Purification (Commentary) (protein specific)
Commentary Organism
-
 Methanosarcina barkeri
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
methanol + 5-hydroxybenzimidazolylcobamide
-
 485660 Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 485660 Methanosarcina barkeri ?
methanol + 5-hydroxybenzimidazolylcobamide one of the enzymes responsible for the transmethylation from methanol to coenzyme M 485660 Methanosarcina barkeri Co-methyl-Co-5-hydroxybenzimidazolylcobamide + H2O
-
 485660 Methanosarcina barkeri ?
Subunits (protein specific)
Subunits Commentary Organism
trimer alpha2,beta, 2 * 34000 + 1 * 53000, SDS-PAGE in presence of 2-mercaptoethanol Methanosarcina barkeri
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Methanosarcina barkeri
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.2
-
 assay at Methanosarcina barkeri

See also following references to EC number 2.1.1.90 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
698630OpulenciaPhysiology and posttranscripti ...Methanosarcina acetivoransJ. Bacteriol.1916928-69352009-------------1------------------------------------------------------1--1--
680470BoseDifferential regulation of the ...Methanosarcina acetivoransJ. Bacteriol.1887274-72832006-----------1-1--------1--------------------------1--------1---------------
682524HagemeierInsight into the mechanism of ...Methanosarcina barkeriProc. Natl. Acad. Sci. USA10318917-189222006---7---------1-------------------------7----------------------------------
681980PritchettGenetic, physiological and bio ...Methanosarcina acetivoransMol. Microbiol.561183-11942005-----------1-4--------1--------------------------1--------1---------------
485665SauerMethanol:coenzyme M methyltran ...Methanosarcina barkeriEur. J. Biochem.249280-2851997--1----1-1---1--------1--------------1-------1-1----------1---------------
485666SauerMethanol:coenzyme M methyltran ...Methanosarcina barkeriEur. J. Biochem.243670-6771997--1----------1--1---1-21-------1-----11--------------1--1-21--------------
485660Van der MeijdenPurification and properties of ...Methanosarcina barkeriJ. Bacteriol.160629-635198414----76--711-31-1-----211---1--1---14--1--7-6---711-1-1----211---1---------
485661Van der MeijdenReductive activation of methan ...Methanosarcina barkeriBiochem. Biophys. Res. Commun.118760-76619847------------1--1------------------7-----------------1--------------------
485663Van der Meijden-Methanol conversion in Eubacte ...Eubacterium limosum, Methanothermobacter thermautotrophicusArch. Microbiol.138360-3641984-------------2------------------------------------------------------------
485662Van der MeijdenMethyltransferases involved in ...Methanosarcina barkeriArch. Microbiol.134238-2421983-------------11----------------1------1------------1----------------------
485664TaylorA simplified assay for coenzym ...Methanobacterium bryantiiJ. Biol. Chem.2494886-48901974-------------1------------------------------------------------------------