Purification and characterization of S-adenosyl-L-methionine: caffeic acid 3-O-methyltransferase from suspension cultures of alfalfa (Medicago sativa L.)

Edwards, R.; Dixon, R.A.; Arch. Biochem. Biophys. 287, 372-379 (1991)

Data extracted from this reference:

Activating Compound

Activating Compound	Commentary	Organism	Structure
2-mercaptoethanol	7% activity increase at 20 mM	Medicago sativa
dithiothreitol	7% activity increase at 20 mM	Medicago sativa

Inhibitors

Inhibitors	Commentary	Organism	Structure
S-adenosyl-L-homocysteine	strong, product inhibition, competitive	Medicago sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.007	-	S-adenosyl-L-methionine	isozyme II	Medicago sativa
0.012	-	S-adenosyl-L-methionine	isozyme I	Medicago sativa
0.053	-	caffeic acid	isozyme I	Medicago sativa
0.059	-	caffeic acid	isozyme II	Medicago sativa

Localization

Localization	Commentary	Organism	GeneOntology No.	Textmining
soluble	-	Medicago sativa	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
41000	-	SDS-PAGE	Medicago sativa

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Medicago sativa	-	elicitor-treated, elicitors are fungal macromolecules from the cell walls of Colletotrichum lindemuthianum, two isoforms	-

Storage Stability

Storage Stability	Organism
-70°C, 2 months, stable	Medicago sativa
4°C, 4-5 d, assay buffer, 50% activity	Medicago sativa

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid	-	485541	Medicago sativa	S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid	-	-	-	?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate	-	485541	Medicago sativa	S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate	i.e. ferulate	485541	Medicago sativa	?

Subunits

Subunits	Commentary	Organism
monomer	1 * 41000, SDS-PAGE	Medicago sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.2	-	-	Medicago sativa

pH Range

pH Minimum	pH Maximum	Commentary	Organism
7	7.4	37% of maximal activity at pH 7.0, 75% of maximal activity at pH 7.4	Medicago sativa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Commentary	Organism	Structure
0.002	-	S-adenosyl-L-homocysteine	isozyme II	Medicago sativa
0.004	-	S-adenosyl-L-homocysteine	isozyme I	Medicago sativa

Activating Compound (protein specific)

Activating Compound	Commentary	Organism	Structure
2-mercaptoethanol	7% activity increase at 20 mM	Medicago sativa
dithiothreitol	7% activity increase at 20 mM	Medicago sativa

Inhibitors (protein specific)

Inhibitors	Commentary	Organism	Structure
S-adenosyl-L-homocysteine	strong, product inhibition, competitive	Medicago sativa

Ki Value [mM] (protein specific)

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Commentary	Organism	Structure
0.002	-	S-adenosyl-L-homocysteine	isozyme II	Medicago sativa
0.004	-	S-adenosyl-L-homocysteine	isozyme I	Medicago sativa

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.007	-	S-adenosyl-L-methionine	isozyme II	Medicago sativa
0.012	-	S-adenosyl-L-methionine	isozyme I	Medicago sativa
0.053	-	caffeic acid	isozyme I	Medicago sativa
0.059	-	caffeic acid	isozyme II	Medicago sativa

Localization (protein specific)

Localization	Commentary	Organism	GeneOntology No.	Textmining
soluble	-	Medicago sativa	-	-

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
41000	-	SDS-PAGE	Medicago sativa

Storage Stability (protein specific)

Storage Stability	Organism
-70°C, 2 months, stable	Medicago sativa
4°C, 4-5 d, assay buffer, 50% activity	Medicago sativa

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid	-	485541	Medicago sativa	S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid	-	-	-	?
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate	-	485541	Medicago sativa	S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate	i.e. ferulate	485541	Medicago sativa	?

Subunits (protein specific)

Subunits	Commentary	Organism
monomer	1 * 41000, SDS-PAGE	Medicago sativa

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.2	-	-	Medicago sativa

pH Range (protein specific)

pH Minimum	pH Maximum	Commentary	Organism
7	7.4	37% of maximal activity at pH 7.0, 75% of maximal activity at pH 7.4	Medicago sativa

See also following references to EC number 2.1.1.68 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
701663	Bhuiya		A cost-effective colorimetric ...	Populus trichocarpa	Anal. Biochem.	384	151-158	2009	-	-	1	-	-	-	-	2	-	-	-	-	-	2	-	-	1	-	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	2	-	2	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
704880	Ma		The expression of caffeic acid ...	Triticum aestivum	J. Exp. Bot.	60	2763-2771	2009	-	-	1	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-
702477	Ma		Characterization of a caffeic ...	Triticum aestivum	Biochimie	90	515-524	2008	-	-	1	-	-	-	-	5	-	-	1	-	-	1	-	-	1	-	-	3	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	5	-	-	1	-	-	-	-	1	-	3	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	5	5
702917	Guillaumie		Expression of cell wall relate ...	Zea mays	BMC Plant Biol.	8	71	2008	-	-	-	-	1	-	-	-	1	-	-	1	-	4	-	-	-	-	-	2	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	1	-	-	-	-	-	2	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
675589	Lin	-	Cloning and functional analysi ...	Oryza sativa	J. Pestic. Sci.	31	47-53	2006	-	-	1	-	-	-	10	2	-	-	2	-	-	1	-	-	1	-	-	3	-	1	6	-	-	-	-	-	1	1	-	-	-	-	-	-	-	1	-	-	-	-	-	10	-	2	-	-	2	-	-	-	-	1	-	3	-	1	6	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-
676547	Li		Evolution of novel O-methyltra ...	Vanilla planifolia, Vanilla pompona	Plant Mol. Biol.	61	537-552	2006	-	1	1	-	-	-	-	2	-	-	2	-	-	5	-	-	1	-	-	4	-	2	8	-	-	-	-	2	-	-	-	-	-	-	-	-	2	2	-	-	-	-	-	-	-	4	-	-	2	-	-	-	-	2	-	7	-	2	12	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-
676553	Fornale		Down-regulation of the maize a ...	Arabidopsis thaliana, Zea mays	Plant Mol. Biol.	62	809-823	2006	-	2	1	-	-	-	-	-	-	-	-	-	-	5	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
660255	Vincent		Water deficits affect caffeate ...	Zea mays	Plant Physiol.	137	949-960	2005	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
658800	Chen	-	Transgenic down-regulation of ...	Festuca arundinacea	Funct. Plant Biol.	31	235-245	2004	-	-	1	-	-	-	-	6	-	-	-	1	-	1	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	6	-	-	-	1	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
660104	Kota		O-Methylation of benzaldehyde ...	Medicago sativa	Phytochemistry	65	837-846	2004	-	1	1	-	11	-	-	41	-	-	-	-	-	4	-	-	-	-	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	1	1	-	-	11	-	-	-	-	41	-	-	-	-	-	-	-	-	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
718207	Pak		Characterization of a multifun ...	Vanilla planifolia	Plant Cell Rep.	22	959-966	2004	-	-	1	-	-	-	-	7	-	-	-	1	-	3	-	-	1	-	-	6	-	-	9	1	-	-	-	-	-	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	7	-	-	-	1	-	-	-	1	-	6	-	-	9	1	-	-	-	-	-	-	-	-	-	1	1	-	-	-
656926	Ruelland	-	Cellular and subcellular local ...	Saccharum officinarum, Zea mays	Physiol. Plant.	117	93-99	2003	-	-	2	-	-	-	-	-	2	-	-	2	-	2	-	-	2	-	-	8	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	2	-	-	2	-	-	-	2	-	8	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485530	Eckardt		Probing the mysteries of ligni ...	Medicago sativa	Plant Cell	14	1185-1189	2002	-	-	-	1	-	-	-	3	-	-	-	1	-	1	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	3	-	-	-	1	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485531	Zubieta		Structural basis for the modul ...	Medicago sativa	Plant Cell	14	1265-1277	2002	-	-	1	1	7	-	-	6	-	-	-	1	-	3	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	7	-	-	-	-	6	-	-	-	1	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485065	Day		Caffeoyl-coenzyme A 3-O-methyl ...	Linum usitatissimum	Physiol. Plant.	113	275-284	2001	-	-	-	-	-	-	-	-	-	-	-	-	-	4	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485067	Parvathi		Substrate preferences of O-met ...	Medicago sativa	Plant J.	25	193-202	2001	-	-	1	-	-	-	-	6	-	-	1	2	-	4	-	-	-	-	-	1	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	6	-	-	1	2	-	-	-	-	-	1	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485539	Ye		Caffeoyl coenzyme A O-methyltr ...	Magnoliophyta, Nicotiana tabacum, Populus tremuloides	Phytochemistry	57	1177-1185	2001	-	-	-	-	-	-	-	-	-	-	-	3	-	4	-	-	-	-	-	-	-	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	5	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485071	Grimmig	-	Expression of bifunctional caf ...	Petroselinum crispum	Plant Biol.	1	299-310	1999	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485072	Martz		cDNA cloning, substrate specif ...	Nicotiana tabacum	Plant Mol. Biol.	36	427-437	1998	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485073	Meng		Substrate profiles and express ...	Populus tremuloides	Plant Mol. Biol.	38	513-520	1998	-	-	1	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	1	4	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	4	-	2	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
485534	Xue	-	Kinetin-induced caffeic acid O ...	Vanilla planifolia	Plant Physiol. Biochem.	36	779-788	1998	-	-	1	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485538	Bugos		Characterization of bispecific ...	Populus tremuloides	Phytochemistry	31	1495-1498	1992	-	-	-	-	-	-	1	-	-	-	1	-	-	1	-	-	1	-	-	1	1	-	3	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	1	-	1	1	-	3	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485540	Bugos		cDNA cloning, sequence analysi ...	Populus tremuloides	Plant Mol. Biol.	17	1203-1215	1991	-	-	1	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485541	Edwards		Purification and characterizat ...	Medicago sativa	Arch. Biochem. Biophys.	287	372-379	1991	2	-	-	-	-	-	1	4	1	-	1	-	-	4	-	-	-	-	-	-	-	2	2	1	-	-	-	-	1	1	-	-	2	-	-	2	-	-	-	-	-	-	-	1	2	4	1	-	1	-	-	-	-	-	-	-	-	2	2	1	-	-	-	-	1	1	-	-	-	-	-	-	-	-
485542	Vance	-	Purification and properties of ...	Medicago sativa	Phytochemistry	20	41-43	1981	-	-	-	-	-	-	1	2	-	-	1	1	-	2	-	-	1	-	-	1	1	-	7	-	-	-	-	-	1	1	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	2	-	-	1	1	-	-	-	1	-	1	1	-	7	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-
485543	Collendavelloo	-	Purification and properties of ...	Nicotiana tabacum	Phytochemistry	20	611-616	1981	-	-	-	-	-	3	-	10	-	1	1	1	-	1	-	-	1	-	-	1	-	1	13	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	10	-	1	1	1	-	-	-	1	-	1	-	1	13	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-
485544	Sugano	-	Effect of 2,4-dichlorophenoxya ...	Daucus carota	Phytochemistry	20	617-619	1981	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485545	Poulton		Enzymic synthesis of lignin pr ...	Glycine max	Arch. Biochem. Biophys.	176	449-456	1976	-	-	-	-	-	-	4	5	-	-	-	-	-	1	-	-	-	1	-	1	1	1	7	-	-	-	-	-	1	1	-	-	1	-	-	-	-	-	-	-	-	-	-	4	1	5	-	-	-	-	-	-	-	-	-	1	1	1	7	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-
485546	Poulton		Purification and properties of ...	Beta vulgaris, Phaseolus vulgaris, Pisum sativum, Spinacia oleracea	Biochim. Biophys. Acta	403	301-314	1975	-	-	-	-	-	1	5	2	-	1	-	4	-	7	-	-	1	-	-	2	1	1	11	-	1	-	-	-	1	1	-	-	1	-	-	-	-	-	-	-	-	1	-	5	1	2	-	1	-	4	-	-	-	1	-	2	1	1	11	-	1	-	-	-	1	1	-	-	-	-	-	-	-	-
485547	Shimada	-	Evidence for the formation of ...	Bambusa sp.	Phytochemistry	12	2873-2875	1973	-	-	-	-	-	-	1	2	-	-	-	-	-	1	-	-	1	-	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	2	-	-	-	-	-	-	-	1	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-