Characterization of osmolyte betaine synthesizing sarcosine dimethylglycine N-methyltransferase from Methanohalophilus portucalensis

Chen, S.; Lai, M.; Lai, S.; Lee, Y.; Arch. Microbiol. 191, 735-743 (2009)

 show all sequences of 2.1.1.157

Data extracted from this reference:

Inhibitors
Inhibitors Commentary Organism Structure
betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
-
additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
-
S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis
0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis FDF1
0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis
0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis FDF1
2.29
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
2.29
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
3.76
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
3.76
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
33000
-
 gel fitlration Methanohalophilus portucalensis FDF1
33000
-
 gel fitlration Methanohalophilus portucalensis
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Methanohalophilus portucalensis
-
-
-
Purification (Commentary)
Commentary Organism
-
 Methanohalophilus portucalensis
-
 Methanohalophilus portucalensis FDF1
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis FDF1
0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis
3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
Storage Stability
Storage Stability Organism
-85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis
-85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis FDF1
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 695924 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine + H+
-
-
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 695924 Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + betaine + H+
-
-
-
 ?
S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine 695924 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine + H+
-
-
-
 ?
S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine 695924 Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N-dimethylglycine + H+
-
-
-
 ?
Subunits
Subunits Commentary Organism
monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis
monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis FDF1
Turnover Number [1/s]
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
0.44
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
0.44
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
2.68
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
2.68
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
pI Value
Organism Commentary pI Value Maximum pI Value
Methanohalophilus portucalensis isoelectric focusing
-
 5
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
betaine above 1.0 M, no effect on sarcosine N-methyltransferase activity, 35% inhibition of N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
-
additional information potassium or sodium ions at 200-1000 mM affect neither sarcosine N-methyltransferase activity nor dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
-
S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
S-adenosyl-L-homocysteine 0.2 mM, 63% loss of sarcosine N-methyltransferase activity, 81% loss of dimethylglycine N-methyltransferase activity. At 1 mM, complete loss of sarcosine N-methyltransferase activity, 88% loss of dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis
0.21
-
 S-adenosyl-L-methionine cosubstrate sarcosine, pH 7.2, 37°C Methanohalophilus portucalensis FDF1
0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis
0.59
-
 S-adenosyl-L-methionine cosubstrate N,N-dimethylglycine, pH 7.2, 37°C Methanohalophilus portucalensis FDF1
2.29
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
2.29
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
3.76
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
3.76
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
33000
-
 gel fitlration Methanohalophilus portucalensis
33000
-
 gel fitlration Methanohalophilus portucalensis FDF1
Purification (Commentary) (protein specific)
Commentary Organism
-
 Methanohalophilus portucalensis
-
 Methanohalophilus portucalensis FDF1
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis
0.71
-
 pH 7.2, 37°C, sarcosine N-methyltransferase activity Methanohalophilus portucalensis FDF1
3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis
3
-
 pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity Methanohalophilus portucalensis FDF1
Storage Stability (protein specific)
Storage Stability Organism
-85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis
-85°C, 5 months, no significant loss of both activities Methanohalophilus portucalensis FDF1
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 695924 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine + H+
-
-
-
 ?
S-adenosyl-L-methionine + N,N-dimethylglycine
-
 695924 Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + betaine + H+
-
-
-
 ?
S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine 695924 Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine + H+
-
-
-
 ?
S-adenosyl-L-methionine + sarcosine about 30% of the activity with N,N-dimethylglycine 695924 Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N-dimethylglycine + H+
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis
monomer 1 * 33000, SDS-PAGE Methanohalophilus portucalensis FDF1
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
0.44
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis
0.44
-
 Sarcosine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
2.68
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
2.68
-
 N,N-Dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis FDF1
pI Value (protein specific)
Organism Commentary pI Value Maximum pI Value
Methanohalophilus portucalensis isoelectric focusing
-
 5

See also following references to EC number 2.1.1.157 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
720836LaiCharacterization and regulatio ...Methanohalophilus portucalensis, Methanohalophilus portucalensis FDF1PLoS ONE6e250902011--2---68-2-6-2--2-----822---1--2--4--22---46-8-2-6---2----822---1---2222--
719715KimuraGlycine betaine biosynthesized ...Myxococcus xanthusJ. Bacteriol.1921467-14702010--1-1------2-2--------21-------1-----11-1--------2--------21---------44---
695387KallioPreliminary X-ray analysis of ...Halorhodospira halochlorisActa Crystallogr. Sect. F65805-8082009--11---------4-----------------------1-1----------------------------------
695924ChenCharacterization of osmolyte b ...Methanohalophilus portucalensisArch. Microbiol.191735-7432009------68--2--3--2---4242---4-----1---------6-8--2----2--4242---4---1------
689964McCoyDiscovery of sarcosine dimethy ...Galdieria sulphurariaProteins74368-3672008--11---4--1--2--1-----21--122--------1-1-----4--1----1----21--122---------
677087LaiEffects of substrate and potas ...Methanohalophilus portucalensis, Methanohalophilus portucalensis FDF1Res. Microbiol.157948-9552006---------2-4-4--------4------------------------2-4--------4---------------
659286WaditeeIsolation and functional chara ...Aphanothece halophyticaJ. Biol. Chem.2784932-494220031-1-3-126--13-1--1-----611---1------1-1--3--12-6--13---1----611---1---------
657578NyyssolaCharacterization of glycine sa ...Halorhodospira halochlorisAppl. Environ. Microbiol.672044-20502001-31---54-113-4--1---3-511---3-------31-----5-4-113---1--3-511---3---------
659145NyyssolaExtreme halophiles synthesize ...Actinopolyspora halophila, Halorhodospira halochlorisJ. Biol. Chem.27522196-222012000--1-------16-6--12--6-1012---2--------1----------16---1--6-1012---2---------