A methyltransferase forsynthesis of the phytoalexin 6-methoxymellein in carrot cells

Kurosaki, F.; Nishi, A.; FEBS Lett. 227, 183-186 (1988)

Data extracted from this reference:

Activating Compound

Activating Compound	Commentary	Organism	Structure
2-Chloroethylphosphonic acid	elicitor, the activity increases after a 2 day lag period and attains a maximum 3 days after the addition, then decreases gradually	Daucus carota
uronide	elicitor	Daucus carota	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
S-adenosyl-L-methionine + 6-hydroxymellein	Daucus carota	enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue	S-adenosyl-L-homocysteine + 6-methoxymellein	-	Daucus carota	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Daucus carota	-	treated with 2-chloroethylphosphonic acid; treated with fungal elicitors	-

Source Tissue

Source Tissue	Commentary	Organism	Textmining
root	-	Daucus carota	-

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	no substrates: umbelliferone, scopoletin	485093	Daucus carota	?	-	-	-	-
More	no substrates: 3,4-dehydro-6-methoxymellein, 6-methoxymellein, esculetin	485093	Daucus carota	?	-	-	-	-
S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein	enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein + 3,4-dehydro-6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein + 3,4-dehydro-6-methoxymellein	-	485093	Daucus carota	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
37	-	assay at	Daucus carota

Activating Compound (protein specific)

Activating Compound	Commentary	Organism	Structure
2-Chloroethylphosphonic acid	elicitor, the activity increases after a 2 day lag period and attains a maximum 3 days after the addition, then decreases gradually	Daucus carota
uronide	elicitor	Daucus carota	-

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
S-adenosyl-L-methionine + 6-hydroxymellein	Daucus carota	enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue	S-adenosyl-L-homocysteine + 6-methoxymellein	-	Daucus carota	?

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
root	-	Daucus carota	-

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
More	no substrates: umbelliferone, scopoletin	485093	Daucus carota	?	-	-	-	-
More	no substrates: 3,4-dehydro-6-methoxymellein, 6-methoxymellein, esculetin	485093	Daucus carota	?	-	-	-	-
S-adenosyl-L-methionine + 3,4-dehydro-6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 3,4-dehydro-6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein	enzyme is involved in the biosynthesis of the phytoalexin 6-methoxymellein in carrot tissue	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein	-	485093	Daucus carota	?
S-adenosyl-L-methionine + 6-hydroxymellein + 3,4-dehydro-6-hydroxymellein	-	485093	Daucus carota	S-adenosyl-L-homocysteine + 6-methoxymellein + 3,4-dehydro-6-methoxymellein	-	485093	Daucus carota	?

Temperature Optimum [°C] (protein specific)

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
37	-	assay at	Daucus carota

See also following references to EC number 2.1.1.108 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
485095	Kurosaki	-	Partial purification and chara ...	Daucus carota	Phytochemistry	41	1023-1027	1996	-	-	-	-	-	-	10	-	-	-	1	2	-	1	-	-	1	1	-	-	1	2	3	-	-	-	-	-	1	-	-	-	2	-	-	-	-	-	-	-	-	-	-	10	2	-	-	-	1	2	-	-	-	1	-	-	1	2	3	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
485096	Marinelli	-	Elicitor induction of enzyme a ...	Daucus carota	Phytochemistry	35	1457-1460	1994	2	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485094	Kurosaki	-	Biosynthesis of dihydroisocoum ...	Daucus carota	Phytochemistry	28	1843-1845	1989	1	-	-	-	-	-	1	-	-	-	-	2	-	1	-	-	-	-	-	1	-	-	4	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	1	1	-	-	-	-	2	-	-	-	-	-	1	-	-	4	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
485093	Kurosaki	-	A methyltransferase forsynthes ...	Daucus carota	FEBS Lett.	227	183-186	1988	2	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	1	-	-	6	-	1	-	-	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	6	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-