Triazaspirodimethoxybenzoyls as selective inhibitors of mycobacterial lipoamide dehydrogenase

Bryk, R.; Arango, N.; Venugopal, A.; Warren, J.D.; Park, Y.H.; Patel, M.S.; Lima, C.D.; Nathan, C.; Biochemistry 49, 1616-1627 (2010)

 show all sequences of 1.8.1.4

Data extracted from this reference:

Cloned(Commentary)
Commentary Organism
expressed in Escherichia coli BL21(DE3) Codon Plus RLI cells Mycobacterium tuberculosis
Crystallization (Commentary)
Crystallization Organism
alone or in complex with inhibitor N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide, hanging drop vapor diffusion method, using 100 mM Tris (pH 8.5), 10 mM NaCl, 11% (w/v) polyethylene glycol 10000, and 15% (v/v) ethylene glycol Mycobacterium tuberculosis
Engineering
Amino acid exchange Commentary Organism
A181V the mutant is not inhibited by N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid compared to the wild type enzyme Mycobacterium tuberculosis
A290R the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
F269R the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
G312A/L313G/L314P/Q315M the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
L314P the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
N209V the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
R147T the mutant is more sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
R347S the mutant is not inhibited by N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid compared to the wild type enzyme Mycobacterium tuberculosis
Inhibitors
Inhibitors Commentary Organism Structure
2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]-decan-3-yl]-N-[3-(trifluoromethyl)benzyl]acetamide
-
 Mycobacterium tuberculosis
-
N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide most potent inhibitor, noncompetitive versus NADH, NAD+, and lipoamide Mycobacterium tuberculosis
-
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Mycobacterium tuberculosis P66004
-
-
Purification (Commentary)
Commentary Organism
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
dihydrolipoamide + NAD+
-
 711225 Mycobacterium tuberculosis lipoamide + NADH
-
-
-
 ?
Cofactor
Cofactor Commentary Organism Structure
FAD
-
 Mycobacterium tuberculosis
NAD+
-
 Mycobacterium tuberculosis
Ki Value [mM]
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.000865
-
 N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide pH and temperature not specified in the publication Mycobacterium tuberculosis
-
IC50 Value
IC50 Value IC50 Value Maximum Commentary Organism Inhibitor Structure
0.0009
-
 pH and temperature not specified in the publication Mycobacterium tuberculosis N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide
-
0.002
-
 pH and temperature not specified in the publication Mycobacterium tuberculosis 2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]-decan-3-yl]-N-[3-(trifluoromethyl)benzyl]acetamide
-
Cloned(Commentary) (protein specific)
Commentary Organism
expressed in Escherichia coli BL21(DE3) Codon Plus RLI cells Mycobacterium tuberculosis
Cofactor (protein specific)
Cofactor Commentary Organism Structure
FAD
-
 Mycobacterium tuberculosis
NAD+
-
 Mycobacterium tuberculosis
Crystallization (Commentary) (protein specific)
Crystallization Organism
alone or in complex with inhibitor N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide, hanging drop vapor diffusion method, using 100 mM Tris (pH 8.5), 10 mM NaCl, 11% (w/v) polyethylene glycol 10000, and 15% (v/v) ethylene glycol Mycobacterium tuberculosis
Engineering (protein specific)
Amino acid exchange Commentary Organism
A181V the mutant is not inhibited by N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid compared to the wild type enzyme Mycobacterium tuberculosis
A290R the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
F269R the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
G312A/L313G/L314P/Q315M the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
L314P the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
N209V the mutant is less sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
R147T the mutant is more sensitive to N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid than the wild type enzyme Mycobacterium tuberculosis
R347S the mutant is not inhibited by N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamid compared to the wild type enzyme Mycobacterium tuberculosis
IC50 Value (protein specific)
IC50 Value IC50 Value Maximum Commentary Organism Inhibitor Structure
0.0009
-
 pH and temperature not specified in the publication Mycobacterium tuberculosis N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide
-
0.002
-
 pH and temperature not specified in the publication Mycobacterium tuberculosis 2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]-decan-3-yl]-N-[3-(trifluoromethyl)benzyl]acetamide
-
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro[4.5]-decan-3-yl]-N-[3-(trifluoromethyl)benzyl]acetamide
-
 Mycobacterium tuberculosis
-
N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide most potent inhibitor, noncompetitive versus NADH, NAD+, and lipoamide Mycobacterium tuberculosis
-
Ki Value [mM] (protein specific)
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.000865
-
 N-(2,4-dichlorophenethyl)-2-[8-(2,4-dimethoxybenzoyl)-4-oxo-1-phenyl-1,3,8-triazaspiro-[4.5]decan-3-yl]acetamide pH and temperature not specified in the publication Mycobacterium tuberculosis
-
Purification (Commentary) (protein specific)
Commentary Organism
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
dihydrolipoamide + NAD+
-
 711225 Mycobacterium tuberculosis lipoamide + NADH
-
-
-
 ?

See also following references to EC number 1.8.1.4 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
711099ParkCharacterization of interactio ...Homo sapiensBiochem. Biophys. Res. Commun.395416-4192010--1-8---1----2--1-----1--------1-----11-8-----1------1----1---------------
711225BrykTriazaspirodimethoxybenzoyls a ...Mycobacterium tuberculosisBiochemistry491616-16272010--118-2------3--1-----1--------21-2--1218-221--------1----1---------------
712087KimRoles of dihydrolipoamide dehy ...Candida albicansFungal Genet. Biol.47782-7882010--------1----4--------1--------2------2-------1-----------1----------22---
712521KurokawaGene cloning and characterizat ...Microbacterium luteolumJ. Biosci. Bioeng.109218-22320101-1----2--1--6--1---2-131--1-11-2---1-12------2--1----1--2-131--1-11---11---
713314ChenDisruption of ptLPD1 or ptLPD2 ...Arabidopsis thalianaPlant Physiol.1531385-13972010--------3----3--------1--------2------2-------3-----------1----------22---
713461HuoCloning and purification of re ...Bombyx moriProtein Expr. Purif.7295-1002010--1----------5--1--12-2--------2-----12--------------1-12-2---------------
703390de MelloEffects of insulin treatment o ...Rattus norvegicusDigest. Dis. Sci.54731-7372009-------------1-------------------------------------------------------11---
711320TyagiMoonlighting protein in Starke ...Starkeyomyces koorchalomoidesBiochimie91868-8752009--1----11-1--3--1--1--1--------2-----12------11-1----1-1--1----------11---
712097StibingerovaLipoamide dehydrogenase and di ...Bos taurus, Sus scrofaGen. Physiol. Biophys.28384-3902009------418-----4-----2--20--------47-7---4---74718---------2--20---------------
713452AmbrusPeriplasmic cold expression an ...Homo sapiensProtein Expr. Purif.6350-572009--1----------3--1-----11-------2-----12--------------1----11---------11---
713453AjithA novel protein that binds to ...Streptomyces peucetiusProtein Expr. Purif.67132-1382009--1-------1--4--1---1-1--------2-----12---------1----1--1-1---------------
685693ChakrabortyTwo proteins with diaphorase a ...Clostridium thermocellum, Moorella thermoacetica ATCC 39073Biosci. Biotechnol. Biochem.72877-8792008-22----4-----2--2-----222------6----226------4-------2----222-------------
690755CastroThe dihydrolipoamide dehydroge ...Aeromonas caviae, Escherichia coli, Geobacillus stearothermophilus, Streptococcus pneumoniae, Zymomonas mobilisBiochem. Biophys. Res. Commun.37591-942008-55-------1--11--5-----5-------------55----------1----5----5---------------
691176KumarHamster sperm capacitation: Ro ...Mesocricetus auratusBiol. Reprod.79190-1992008-1----1------2-----1----------------1------1-----------1------------------
691392ChakrabortyCharacterization of a dihydrol ...Clostridium kluyveriBiosci. Biotechnol. Biochem.72982-9882008-11---36-11--4--1----1411-1-1114----114----3-6-11----1---1411-1-111-------
692385BatistaThe dihydrolipoamide dehydroge ...Acidianus ambivalensFEMS Microbiol. Lett.281147-1542008-1----14--1--3------1-211---1--4----1-4----1-4--1-------1-211---1---------
692858KimDihydrolipoamide dehydrogenase ...Escherichia coli K-12J. Bacteriol.1903851-38582008-11-2-261----3--1-----2--------23---112-2--2361------1----2---------------
693271WangThe role of amino acids T148 a ...Homo sapiensJ. Biomed. Sci.1537-462008-11-4--9--1--3--1-----11---5---1----111-4----9--1----1----11---5----------
693975YanChanges in dihydrolipoamide de ...Rattus norvegicusMech. Ageing Dev.129282-290200811----1-1----3-----7--1------------11------1--1--------7--1---------------
694361DhanasekaranRole of lipoamide dehydrogenas ...Mus musculusNeurochem. Res.33980-984200811----4------4--------1--------1---11-1----4--------------1---------------
702739ChakrabortyCloning and expression of a Cl ...Clostridium kluyveriBiosci. Biotechnol. Biochem.72735-7412008--1----4-14--1--1-----211-1-1113-----13------4-14----1----211-1-111-------
706478KikuchiGlycine cleavage system: react ...no activity in Escherichia coli, Pisum sativumProc. Jpn. Acad. Ser. B Phys. Biol. Sci.84246-2632008-------------5-------------------------------------------------------11---
673517TaharaDihydrolipoyl dehydrogenase as ...Saccharomyces cerevisiaeFASEB J.21274-2832007-1--1-1-1----3--------2--------2----1-2-1--1--1-----------2---------------
674943WangThe role of N286 and D320 in t ...Homo sapiensJ. Biomed. Sci.14203-2102007-11-3--16--1--2--1---5-41---8---3----113-3----16--1----1--5-41---8----------
691036LuisValproic acid metabolites inhi ...Rattus norvegicusBiochim. Biophys. Acta17671126-11332007-1----2-1----2-----2--1---------2---1------22-1--------2--1---------------
691569Kim-Site-specific modifications of ...Homo sapiensBull. Korean Chem. Soc.28907-9082007-11-2--------1--1-----1--------2----112-2------------1----1---------------
692060YanHistochemical staining and qua ...Rattus norvegicusElectrophoresis281036-1045200711----6-2----4--1--7--1--------2---11-2----6--2------1-7--1---------------
693005HakanssonEnzymatic characterization of ...Streptococcus pneumoniaeJ. Biol. Chem.28229521-295302007-11-3-36--1--3--1-----3----32--412---114-3--3126--1----1----3----32---------
693307DeghmaneLipoamide dehydrogenase mediat ...Mycobacterium bovis BCG, Mycobacterium tuberculosis, no activity in Mycobacterium smegmatisJ. Cell Sci.1202796-28062007-21---2------6---------2---------2--21-----2---------------2-------2------
693386AndersonTime course of nicotinamide ad ...Sus scrofaJ. Endourol.21223-2272007-1-----------3-----1----------------1------------------1------------------
694356BrownTesting for linkage and associ ...Homo sapiensNeurochem. Res.32857-8692007-1------1----3----------------------1---------1---------------------------
694854BabadyCryptic proteolytic activity o ...Homo sapiens, Mus musculus, Sus scrofaProc. Natl. Acad. Sci. USA1046158-61632007211-5-3-2----7--2--2--4-----------1211--5-13--2------2-2--4---------------
672869Kim-Examination of the importance ...Homo sapiensBull. Korean Chem. Soc.27819-8202006--1-1--------1--1---1-1--------2-----12-1------------1--1-1---------------
673190Gutierrez-CorreaTrypanosoma cruzi dihydrolipoa ...Trypanosoma cruziCurr. Drug Targets71155-11792006--1---12---1--5--------21-------3-----13----12----1---------21--------------
673991HudsonIdentification of a virulence- ...Mycoplasma gallisepticumInfect. Immun.74931-9392006----1--------4--------------------------1---------------------------------
674368NishimotoThermal unfolding process of d ...Geobacillus stearothermophilusJ. Biochem.140349-3572006-------------2--1-----1--11----2------2--------------1----1--11-----------
674382KimActivity of human dihydrolipoa ...Homo sapiensJ. Biochem. Mol. Biol.39223-2272006--1-1--------4--1-----1--------1-----11-1------------1----1---------------
674759CiszakHow dihydrolipoamide dehydroge ...Homo sapiensJ. Biol. Chem.281648-6552006--11----1----3--1-----11-------2-----121------1------1----11--------------
677161BrautigamStructural insight into intera ...Homo sapiensStructure14611-6212006---14---1----2--1-----11-------2------214-----1------1----11--------------
656835McMillanThe human malaria parasite Pla ...Plasmodium falciparumMol. Microbiol.5527-382005--1----53--1-4-111-1--22---42--3-----13------53--1--11-1--22---42---------
659101KimAsparagine-473 residue is impo ...Homo sapiensJ. Biochem. Mol. Biol.38248-2522005--1-1------1-5--11--1-111---1--2-----12-1--------1---1--1-111---1---------
659482MitraNovelty of the pyruvate metabo ...Mesocricetus auratusJ. Biol. Chem.28025743-257532005--------4--2-2-1---41-5--------2------2-------4--2--1--41-5---------------
672465Ivanova-Drosophila dihydrolipoamide de ...Drosophila melanogaster, Drosophila virilisBiokhimiya, Biofizika, Molekulyarnaya Biologiya i Genetika, Mikrobiologiya i Fiziologiya96115-1232005-------------2--------2--------4------4-------------------2---------------
673943OdievreA novel mutation in the dihydr ...Homo sapiensHum. Mutat.25323-3242005----1--------3-----2--1--------1------1-1--------------2--1---------------
674430KlyachkopH-dependent substrate prefere ...Sus scrofaJ. Biol. Chem.28016106-161142005--------1----3--1--2--13----2--2------2-------1------1-2--13----2---------
674479RajashankarCrystal structure and function ...Mycobacterium tuberculosisJ. Biol. Chem.28033977-339832005--1122--------2--1-----1--------1-----11122------------1----1---------------
675350BrautigamCrystal structure of human dih ...Homo sapiensJ. Mol. Biol.350543-5522005-1119---2----4--1-----11-------3----11319-----2------1----11--------------
658249MitraNovel tyrosine-phosphorylated ...Mesocricetus auratusBiol. Reprod.70887-8992004------1-1--1-2-1---2--12-------2-1----2----1--1--1--1--2--12-------1------
658742IgamberdievDihydrolipoamide dehydrogenase ...Sus scrofaFEBS Lett.568146-15020041-----22---1-4--1--510-81----21-3---1--3----2-2---1---1-510-81----21--------
659866BjoernstedtExtramitochondrial reduction o ...Sus scrofaMethods Enzymol.378131-1382004--------31-1-1--1--1--211---21-3------3-------31-1---1-1--211---21--------
657980ArgyrouCatalysis of diaphorase reacti ...Mycobacterium tuberculosisBiochemistry422218-22282003-------1---1-4---1--1-9-1--31--4------4------1---1------1-9-1--31---------
658231NordmanRegeneration of the antioxidan ...Sus scrofaBiofactors1845-502003--------2111-2-----2--21----1--3------3-------2111-----2--21----1---------
658799Gutierrez-CorreaPhenothiazine radicals inactiv ...Trypanosoma cruziFree Radic. Res.37281-2912003--1---13------5------111-1---1--1-----11----13------------111-1---1---------
659611Hiromasa-Denaturation of the Bacillus s ...Geobacillus stearothermophilusJ. Fac. Agric. Kyushu Univ.47387-39420034-----1------1----1------------1---4--1----1----------1-------------------
394002MoranMolecular cloning, functional ...Glycine maxPlant Physiol.128300-3132002--1----31----5--1--11-21-------3-----13------31------1-11-21--------------
394005SmithCharacterization of the dihydr ...Streptococcus pneumoniaeMol. Microbiol.44431-4482002-----------1-4--------2--------1------1----------1--------2---------------
657921ArgyrouThe lipoamide dehydrogenase fr ...Mycobacterium tuberculosisBiochemistry4114580-145902002-------1---1-3---1----2--1-11--4------4------1---1--------2--1-11---------
658431ChenLipoyl dehydrogenase catalyzes ...Clostridium kluyveri, Sus scrofaChem. Biol. Interact.140199-21320022----------2-5-----3--22-2---2--7---2--7----------2-----3--22-2---2---------
658466WuProteinase 3 and dihydrolipoam ...Homo sapiensClin. Exp. Immunol.128347-3522002-----------1-4--------1--------------------------1--------1---------------
659099KimActivity of human dihydrolipoa ...Homo sapiensJ. Biochem. Mol. Biol.35437-4412002--1-1--------4--1---1--11---1--1-----11-1------------1--1--11---1---------
659167GazaryanZinc is a potent inhibitor of ...Sus scrofaJ. Biol. Chem.27710064-1007220021-----21-----3--11-2--3-1---1--31--1--3----211-------1-2--3-1---1---------
394006ArgyrouMycobacterium tuberculosis lip ...Mycobacterium tuberculosisBiochemistry4011353-113632001--1----16-----5--11----10----1---2-----12------16-------1----10----1----------
394010SchwindeLipoamide dehydrogenase from C ...Corynebacterium glutamicumMicrobiology1472223-22312001--1--1-----1-3--1---114-1---1--2-----12--1-------1---1--114-1---1---------
658600XiaReduction of ubiquinone by lip ...Sus scrofaEur. J. Biochem.2681486-14902001------1--211-2-----22-111--22--2------2----1---211-----22-111--22---------
394011NeuburgerInteraction between the lipoam ...Pisum sativumEur. J. Biochem.2672882-28892000-------2---1-2-----1--31---3---1------1------2---1-----1--31---3----------
394012FaureInteraction between the lipoam ...Pisum sativumEur. J. Biochem.2672890-28982000---1---------4-----1--1--------1------11---------------1--1---------------
394004LiuSite-directed mutagenesis of h ...Homo sapiensProtein Expr. Purif.1627-391999--1-3--12-----4--1-----1----13---2-----12-3----12-------1----1----13----------
393969ToyodaCrystallization and preliminar ...Eubacterium acidaminophilum, Sus scrofaActa Crystallogr. Sect. D54982-9851998---1---------3-----1--2--------2------21---------------1--2---------------
393970ToyodaCrystal structure of eucaryoti ...Saccharomyces cerevisiaeJ. Biochem.123668-6741998---1---------3--------11-------1------11------------------11--------------
394009YounLipoamide dehydrogenase from S ...Streptomyces seoulensisBiochim. Biophys. Acta1388405-4181998--1---13--1--5--11--1-71---3---3-----13----1-3--1----1--1-71---3----------
394007MarcinkevicieneCatalytic properties of lipoam ...Mycobacterium smegmatis, Pisum sativumArch. Biochem. Biophys.340168-1761997------35-----6--11--1-44-------3------3----3-5-------1--1-44--------------
394008EngelsIsolation, partial characteriz ...Synechocystis sp.Biochim. Biophys. Acta134033-441997--------1-1--5--1---1-11-------2------2-------1-1----1--1-11--------------
393994ConnerIdentification and purificatio ...Escherichia coli, Pisum sativumPlanta200195-2021996------122----4--1--22-22-------22-----2----1222------1-22-22--------------
393989HopkinsCharacterization of lipoamide ...Escherichia coliBiochemistry3411757-117651995----2--------1--------5--------3------3-2-----------------5---------------
394003LiuSpectroscopic studies of the c ...Homo sapiensJ. Biol. Chem.27015545-155501995--1-3--7-----4--1-----1----1---2-----12-3----7-------1----1----1----------
393992Maeda-YoritaModulation of the Oxidation-Re ...Escherichia coliBiochemistry336213-62201994----1--------2--------1--------1------1-1-----------------1---------------
393973MatteviThree-dimensional structure of ...Azotobacter vinelandii, Pseudomonas fluorescensJ. Mol. Biol.2301200-12151993---1---------6--------2---1----2------21------------------2---1-----------
486168OppermannPurification and characterizat ...Pelobacter carbinolicusJ. Bacteriol.173757-7671991-------4--1--7--1---1171----1--5------5------4--1----1--1171----1---------
393962LohrerPurification and characterizat ...Trypanosoma cruziEur. J. Biochem.194863-8691990------12-----6--1--1--11-------2------2----1-2-------1-1--11--------------
393963BorgesCloning and sequence analysis ...Geobacillus stearothermophilusEur. J. Biochem.19495-1021990--1-----2----4--------1--------1-----11-------2-----------1---------------
393964DietrichsPurification and comparative s ...Clostridium cylindrosporum, Peptostreptococcus glycinophilusJ. Bacteriol.172243-2511990-------6--2--8--2---2232----4--8------8------6--2----2--2232----4---------
393965JackmanSubcellular localisation of di ...Trypanosoma bruceiEur. J. Biochem.19391-951990--------3----4-----5--1--------1------1-------3--------5--1---------------
393966RicharmePurification of a new dihydrol ...Escherichia coliJ. Bacteriol.1716580-65851989-------21-11-3--1---1-21-------1------1------21-11---1--1-21--------------
393971CarothersDihydrolipoamide dehydrogenase ...Ascaris suum, Azotobacter vinelandii, Bacillus subtilis, Bos taurus, Escherichia coli, Geobacillus stearothermophilus, Halobacterium salinarum, Homo sapiens, Pisum sativum, Pseudomonas aeruginosa, Pseudomonas putida, Rattus norvegicus, Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Sus scrofaArch. Biochem. Biophys.268409-4251989-----------15-22-----9--3011-------15------15----------15-----9--3011--------------
349054SokatchPurification of branched-chain ...Pseudomonas aeruginosa, Pseudomonas putidaMethods Enzymol.166342-3501988-------------2--1---1-2--------2------2--------------1--1-2---------------
392950SundquistThe novel disulfide reductase ...Halobacterium salinarumJ. Bacteriol.1703459-34671988-------2-----3--1-----31--1----2------2------2-------1----31--1-----------
393968PerhamIsolation and properties of th ...Bacillus subtilisMethods Enzymol.166330-3421988-------------1--1-----1--------1------1--------------1----1---------------
349067OnoPurification, resolution, and ...Rattus norvegicusJ. Biochem.10119-271987--------1----2--1--11-1--------1------1-------1------1-11-1---------------
393974Smith-Dihydrolipoamide dehydrogenase ...Thermoplasma acidophilumBiochem. Soc. Trans.1510971987------12--11-1------1-2--------1------1----1-2--11------1-2---------------
393975RoyCloning and characterization o ...Haloferax volcanii, Saccharomyces cerevisiaeJ. Gen. Microbiol.133925-9331987--1----------4--------2--------2-----12-------------------2---------------
393976DansonDihydrolipoamide dehydrogenase ...Trypanosoma cruziBiochem. J.243661-6651987------121----3--1--21-1--------1------1----1-21------1-21-1---------------
393978Danson-Dihydrolipoamide dehydrogenase ...Halobacterium salinarumBiochemistry253880-38841986------1--1---1--11--1-11-------2------2----1---1-----1--1-11--------------
348939BosmaThe composition of the pyruvat ...Azotobacter vinelandiiEur. J. Biochem.142541-5491984----------1--1--1-----11-------1------1---------1----1----11--------------
393980DelaneyRelationship of lipoamide dehy ...Pseudomonas putidaFEBS Lett.168265-2701984----------21-5--------2--------2------2---------21--------2---------------
393981DansonDihydrolipoamide dehydrogenase ...Halobacterium salinarum, Haloferax volcanii, Natronobacterium gregoryi, Natronococcus occultus, Natronomonas pharaonisBiochem. J.218811-8181984------12--5--7--------5---8----11------11----1-2--5---------5---8-----------
348944HodgsonWild-type and mutant forms of ...Bacillus subtilisBiochem. J.211463-4721983-----1--2-1--3--1---1111-------1------1--1----2-1----1--1111--------------
393982TsaiMultifunctionality of lipoamid ...Sus scrofaArch. Biochem. Biophys.225554-5611983-------------3-----1--2--------2------2----------------1--2---------------
393984HeinrichLipoamide dehydrogenase from b ...Saccharomyces cerevisiaeHoppe-Seyler's Z. Physiol. Chem.36441-501983-----112--1--3--1---1-31----1--31-----3--1-112--1----1--1-31----1---------
348948SecklerPurification and properties of ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochim. Biophys. Acta705210-2171982-------------4--1-----11-------1------1--------------1----11--------------
393986MatudaIntracellular distribution and ...Rattus norvegicusJ. Biochem.91553-5611982--------5-1--3-----21-11-------2------2-------5-1------21-11--------------
393987Adamson-Inhibition of pyruvate dehydro ...Escherichia coliBiochemistry203420-34241981------1------1--------1--------1------1----1--------------1---------------
393988Schmincke-OttDihydrolipoamide dehydrogenase ...Escherichia coliEur. J. Biochem.114413-4201981------142----3--11--1-11---111-22-----2----1242------1--1-11---111--------
393990McKayLipoamide dehydrogenase from M ...Malbranchea pulchellaBiochemistry184702-47071979----------1--3--1--21-21--4-11-2------2---------1----1-21-21--4-11--------
393991KomunieckiPurification of lipoamide dehy ...Ascaris suumArch. Biochem. Biophys.196239-2471979------142-1--2--1--21151----3--3------3----1-42-1----1-21151----3---------
393993Williams-Flavin-containing dehydrogenas ...Azotobacter agilis, Azotobacter vinelandii, Bacillus subtilis, Bos taurus, Brassica oleracea, Enterococcus faecalis, Escherichia coli, Homo sapiens, Leuconostoc mesenteroides, Mycobacterium tuberculosis, Neurospora crassa, Parvimonas micra, Phytophthora erythroseptica, Pichia kudriavzevii, Proteus vulgaris, Pseudomonas fluorescens, Pythium ultimum, Rattus norvegicus, Saccharomyces cerevisiae, Serratia marcescens, Spinacia oleracea, Squalus acanthias, Sus scrofaThe Enzymes, 3rd Ed. (Boyer, P.D., ed.)1389-1731976-------------24-----10--23--------23------23----------------10--23---------------
393995WaisIsolation and characterisation ...Saccharomyces carlsbergensisHoppe-Seyler's Z. Physiol. Chem.3541378-13881973-------------1--------11-------1------1-------------------11--------------
393996ScoutenPurification of lipoamide dehy ...Saccharomyces cerevisiae, Sus scrofaBiochim. Biophys. Acta309521-5241973-------------2--2-----2--------2------2--------------2----2---------------
393999ScoutenMicrobial lipoamide dehydrogen ...Azotobacter agilis, Bacillus subtilis, Escherichia coli, Neurospora crassa, Pseudomonas fluorescens, Saccharomyces cerevisiae, Serratia marcescensBiochim. Biophys. Acta227248-2631971------2------7------5-7--------7------7----2------------5-7---------------
394000MillardBrain lipoyl dehydrogenase. Pu ...Sus scrofaJ. Biol. Chem.2442511-25151969------5------1--1--11-2--------1------1----5---------1-11-2---------------
394001Jacobi-Isolation und Eigenschaften vo ...Spinacia oleraceaZ. Pflanzenphysiol.58193-20619682-----3211---1--1--1--4--------2---2--2----3-211-----1-1--4---------------
348981Reed-Purification and resolution of ...Escherichia coliMethods Enzymol.9247-2651966------2------1--------1--------2------2----2--------------1---------------
393998KawaharaPurification and properties of ...Pichia kudriavzeviiJ. Biochem.6377-821963---1--1------1--1---1-3-----22-3------31---1---------1--1-3-----22--------