Strengthening intersubunit hydrogen bonds for enhanced stability of recombinant urate oxidase from Aspergillus flavus: molecular simulations and experimental validation

Liu, Z.; Lu, D.; Li, J.; Chen, W.; Liu, Z.; Phys. Chem. Chem. Phys. 11, 333-340 (2009)

 show all sequences of 1.7.3.3

Data extracted from this reference:

Application
Application Commentary Organism
medicine substitute for allopurinol in the management of gout and hyperuricaemia Aspergillus flavus
Cloned(Commentary)
Commentary Organism
expressed in Escherichia coli BL21 (DE3) Aspergillus flavus
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
urate + O2 + H2O Aspergillus flavus first step in uric acid degradation 5-hydroxyisourate + H2O2
-
-
 ?
Organic Solvent Stability
Organic Solvent Commentary Organism
DMSO the residual recombinant UOX activity in the presence of DMSO is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the DMSO concentration up to 20%, further increase in DMSO concentration (50-70%) results in significant UOX deactivation Aspergillus flavus
Methanol the residual recombinant UOX activity in the presence of methanol is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the methanol concentration up to 20%, further increase in methanol concentration (50-70%) results in significant UOX deactivation Aspergillus flavus
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Aspergillus flavus Q00511
-
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
urate + O2 + H2O first step in uric acid degradation 700631 Aspergillus flavus 5-hydroxyisourate + H2O2
-
-
-
 ?
Subunits
Subunits Commentary Organism
homotetramer determined by gel filtration, tunnel-shaped homotetramer, each of the 4 active sites in UOX is formed by residues from 2 subunits and located at the subunit-subunit interface Aspergillus flavus
Temperature Stability [°C]
Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Commentary Organism
additional information
-
 thermal deactivation of recombinant UOX at neutral pH is associated with the loss of intersubunit hydrogen bonds, subunit is unstable at room temperature and unfolds rapidly at 100°C, tetramer has significantly higher stability than its subunit Aspergillus flavus
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.9
-
 assay at Aspergillus flavus
pH Stability
pH Stability pH Stability Maximum Commentary Organism
7
-
 UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml Aspergillus flavus
Application (protein specific)
Application Commentary Organism
medicine substitute for allopurinol in the management of gout and hyperuricaemia Aspergillus flavus
Cloned(Commentary) (protein specific)
Commentary Organism
expressed in Escherichia coli BL21 (DE3) Aspergillus flavus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
urate + O2 + H2O Aspergillus flavus first step in uric acid degradation 5-hydroxyisourate + H2O2
-
-
 ?
Organic Solvent Stability (protein specific)
Organic Solvent Commentary Organism
DMSO the residual recombinant UOX activity in the presence of DMSO is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the DMSO concentration up to 20%, further increase in DMSO concentration (50-70%) results in significant UOX deactivation Aspergillus flavus
Methanol the residual recombinant UOX activity in the presence of methanol is significantly higher than that in pure H2O, the residual UOX activity increases in response to the increase in the methanol concentration up to 20%, further increase in methanol concentration (50-70%) results in significant UOX deactivation Aspergillus flavus
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
urate + O2 + H2O first step in uric acid degradation 700631 Aspergillus flavus 5-hydroxyisourate + H2O2
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
homotetramer determined by gel filtration, tunnel-shaped homotetramer, each of the 4 active sites in UOX is formed by residues from 2 subunits and located at the subunit-subunit interface Aspergillus flavus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Commentary Organism
additional information
-
 thermal deactivation of recombinant UOX at neutral pH is associated with the loss of intersubunit hydrogen bonds, subunit is unstable at room temperature and unfolds rapidly at 100°C, tetramer has significantly higher stability than its subunit Aspergillus flavus
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
8.9
-
 assay at Aspergillus flavus
pH Stability (protein specific)
pH Stability pH Stability Maximum Commentary Organism
7
-
 UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml Aspergillus flavus

See also following references to EC number 1.7.3.3 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
710719CollingsPolymorphism of microcrystalli ...Aspergillus flavusActa Crystallogr. Sect. D66539-5482010--11--1---1--3--1------1-------------1-1---1----1----1-----1--------------
711515ZhangEffects of modification of ami ...Bacillus fastidiosusBiosci. Biotechnol. Biochem.741298-13012010--1--162252--4--1-----1---1-1---3----1---1-632252----1----1---1-1---------
712197TanCharacterization, stabilizatio ...Cyberlindnera jadiniiInt. J. Pharm.384165-1722010-----13------1-------21-111-111----------1-3-------------21-111-111-------
712513PfrimerCloning, purification, and par ...Bacillus subtilisJ. Biomed. Biotechnol.20106749082010--1-------3--5--1---231-1-1-11-------1----------3----1--231-1-1-11--------
699832OksanenLarge crystal growth by therma ...Aspergillus flavusJ. R. Soc. Interface6 Suppl 5599-6102009-1-1--2---11-3-----1--11------------1--1---2----11-----1--11--------------
700631LiuStrengthening intersubunit hyd ...Aspergillus flavusPhys. Chem. Chem. Phys.11333-3402009-11--------123--------11--1-1-1-----11-----------12-------11--1-1-1-------
711510ZhaoReversible inactivation of an ...Bacillus fastidiosusBiosci. Biotechnol. Biochem.732141-21442009-----1--1-1--3--------11-----------------1----1-1---------11--------------
695394JuanStructures of Arthrobacter glo ...Arthrobacter globiformisACTA CRYSTALLOGR.SECT.D64815-8222008--11--1----1-3--1-----11-------1-----111---1-----1---1----11--------------
695795ChenUricase production by a recomb ...Cyberlindnera jadiniiAppl. Microbiol. Biotechnol.79545-5542008-11-------12-5-----11-2-----2-1-----11----------12-----11-2-----2-1-------
696583PessionPitfalls, prevention, and trea ...Saccharomyces cerevisiaeBiologics2129-1412008-11--------2-2------1-2-------------11-----------2------1-2---------------
696770Colloc'hOxygen pressurized X-ray cryst ...Aspergillus flavusBiophys. J.952415-24222008--11--1--1-1-2---1----2--------1-----111---1---1-1--------2---------------
696795LotfyProduction of a thermostable u ...Geobacillus thermocatenulatusBiores. Technol.99699-702200811-----1---1-3------1-1---2-2------11--------1---1------1-1---2-2---------
697005GabisonStructural analysis of urate o ...Aspergillus flavusBMC Struct. Biol.8322008---1--1----1-2---1----2----------------1---1-----1--------2---------------
700977ScaraffiaDiscovery of an alternate meta ...Aedes aegyptiProc. Natl. Acad. Sci. USA105518-52320081-----1------4-----2---------------1-------1-----------2------------------
701100ChenSite-specific incorporation of ...Cyberlindnera jadiniiProtein Sci.171827-18332008-11--------1-3--1---3-1-1---1-------11-----------1---1--3-1-1---1---------
701416Kai-Purification and characterizat ...Microbacterium sp.World J. Microbiol. Biotechnol.24401-4062008------61-2-1-2--1---1-111131113------------6-1-2-1---1--1-111131113-------
695952SundyPharmacokinetics and pharmacod ...Sus scrofaArthritis Rheum.561021-10282007-1--1--------2----------------------1---1---------------------------------
671106Budayova-SpanoA preliminary neutron diffract ...Aspergillus flavusActa crystallogr. Sect. F62306-3092006--11---------3--1-----1--------------1-1-------------1----1---------------
672398CollochHigh pressure macromolecular c ...Aspergillus flavusBiochim. Biophys. Acta1764391-3972006---1--1---1--3--------11---------------1---1----1---------11--------------
672697ZhaoCharacterization of an uricase ...Bacillus fastidiosusBiotechnol. Appl. Biochem.4575-802006------111-1--2-----1-211----11--1----------1111-1------1-211----11--------
673545AndersenPurine-induced expression of u ...Gadus morhua, Hippoglossus hippoglossus, Protopterus annectens, Salmo salarFEBS J.2732839-28502006--------4----14-----10--4-----------------------4--------10--4---------------
673643GabisonRecapture of [S]-allantoin, th ...Aspergillus flavusFEBS Lett.5802087-20912006--11---------3--------2--------------1-1------------------2---------------
676968LiHigh-level expression, purific ...Aspergillus flavusProtein Expr. Purif.4955-592006--1-------1--4--1---4-1-1---1----1---1----------1----1--4-1-1---1--1------
676997RajokaPurification, and properties o ...Bos taurusProtein Pept. Lett.13363-3682006-------------2--1--1--1------------------------------1-1--1---------------
677261Rajoka-Purification and characterizat ...Capra sp.World J. Microbiol. Biotechnol.22289-2912006---------1---1--1--1----111-1------------------1-----1-1----111-1---------
657470RetailleauUrate oxidase from Aspergillus ...Aspergillus flavusActa Crystallogr. Sect. D61218-2292005--11---------4--1--------------------1-1-------------1--------------------
671971DollProcatalytic ligand strain. Io ...Bacillus subtilisBiochemistry4411440-114462005--1-3-14-----2--------1---------1----1--3--114------------1---------------
675091PanCharacterization of the degrad ...Rattus norvegicusJ. Electron Microsc.54385-3922005--1-----1----4-----------------------1--------1---------------------------
676914Abdel-Fattah-Improved production of Pseudom ...Pseudomonas aeruginosaProcess Biochem.401707-17142005---------2---1------1-------1------------------2--------1-------1---------
657465RetailleauComplexed and ligand-free high ...Aspergillus flavusActa Crystallogr. Sect. D60453-4622004---1---------2-------------------------1----------------------------------
658651HuangModified colorimetric assay fo ...Bacillus subtilisEur. J. Biochem.271517-5232004-11----------4--1------1------------11---------------1-----1--------------
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660078Redondo-Nevado-Structural and genomic organiz ...Cicer arietinumPhysiol. Plant.121358-3682004--1----2-----1-----2--1-----1----1---1-------2---------2--1-----1--1------
660334SaeedPurification and characterizat ...Pseudomonas aeruginosaPol. J. Microbiol.5345-522004------5-12---3--1-----111---1--------------5--12-----1----111---1---------
657736HuangAltered antigenic disposition ...Cricetulus griseusBiochem. Biophys. Res. Commun.302703-7092003--------3----4-----2--------------------------3--------2------------------
657986ImhoffGeneral base catalysis in the ...Bacillus subtilisBiochemistry424094-41002003--1-5--6-----3--1-----1--------------1--5----6-------1----1---------------
660337KimProduction of an egg yolk anti ...Arthrobacter globiformisPoult. Sci.821554-15582003------1------3-----------------------------1------------------------------
394181Nishiya-A purification method of the d ...Bacillus sp.Protein Expr. Purif.25426.2002-11----2---1-1--1---1-1-------------11-------2---1---1--1-1---------------
394183AguilarUrate oxidase from the rust Pu ...Puccinia reconditaCurr. Microbiol.44257-2612002------21--11-3--1---1-11----1--------------2-1--11---1--1-11----1---------
394191VivaresX-ray scattering studies of As ...Aspergillus flavusActa Crystallogr. Sect. D58472-4792002---1------1--2---------1---------------1--------1----------1--------------
657529FraisseA colorimetric 96-well microti ...Aspergillus flavusAnal. Biochem.309173-1792002-1-----1-----3--------1----1--------1--------1------------1----1----------
658316BayolModification of a reactive cys ...Aspergillus flavusBiotechnol. Appl. Biochem.3621-312002---1---------6---------1---------2-----2-------------------1-------2------
658371FarleyRegulation of expression of th ...Rhizopus oryzaeCan. J. Microbiol.481104-11082002--------2----6-----1--------------------------2--------1------------------
659863TiptonUrate oxidase:single-turnover ...Glycine maxMethods Enzymol.354310-3192002-------------1-----1--1--------------------------------1--1---------------
660070Montalbini-Purification, quantification a ...Phaseolus vulgarisPhysiol. Mol. Plant Pathol.61141-1502002-------------1--1--11--------------------------------1-11-----------------
394179HongohCloning, sequence analysis and ...yeast-like fungiInsect Biochem. Mol. Biol.30173-1822000--1-------11-5--1----11--------------1----------11---1---11---------------
394185SchiavonTherapeutic proteins: a compar ...Bacillus fastidiosusFarmaco55264-2692000----11-2---1-3--------1---2-------------11---2---1--------1---2-----------
394190Montalbini-Isolation and characterization ...Phaseolus vulgaris, Uromyces phaseoli, Uromyces viciae-fabae, Vicia fabaPlant Sci.147139-1471999------84--44-4--3--43-4------4-------------8-4--44---3-43-4------4--------
394184KahnSpectroscopic Characterization ...Glycine maxBiochemistry3711651-116591998--1-1-----11-3--1-----11---1---------1--1-------11---1----11---1----------
394180KahnKinetic mechanism and cofactor ...Glycine maxBiochemistry364731-47381997--1---22--21-4--1--2111--------12----11----222--21---1-2111---------------
394187Kahn-Identification of the true pro ...Glycine maxJ. Am. Chem. Soc.1195435-54421997--1--------1-1-----1--1--------------1-----------1-----1--1---------------
394188Montalbini-Uricase from leaves. Its purif ...Cicer arietinum, Triticum aestivum, Vicia fabaPlanta202277-2831997------9---33-3--3--3--33-------------------9----33---3-3--33--------------
394189Capote-MainezCharacterization of the common ...Phaseolus vulgarisPlant Physiol.1151307-13171997-----------1-5-----64-1--------------------------1-----64-1---------------
394186Leal-PintoReconstitution of hepatic uric ...Rattus norvegicus, Sus scrofaJ. Membr. Biol.146123-1321995------2-1--2-5-----4--4--------------------2--1--2-----4--4---------------
394182JianguoPurification and properties of ...Candida sp.Appl. Biochem. Biotechnol.4757-631994-1----4---11-2--1--11-111-1-11------1------4----11---1-11-111-1-11--------
394130YeldandiHuman urate oxidase gene: clon ...Homo sapiensBiochem. Biophys. Res. Commun.171641-6461990--1--------1-3--------1--------------1-----------1--------1---------------
394131MotojimaCloning of rabbit uricase cDNA ...Oryctolagus cuniculusBiochim. Biophys. Acta1008116-1181989--1--------1-3-----2--1--------------1-----------1-----2--1---------------
394132AdamekPurification of microbial uric ...Cyberlindnera jadiniiJ. Chromatogr.497268-2751989--------1-11-1--1-----1-1---1-1---------------1-11---1----1-1---1-1-------
394133MotojimaCloning and sequence analysis ...Rattus norvegicusJ. Biol. Chem.26316677-166811988--1-------11-4-----2--1--------------1----------11-----2--1---------------
394134LeeGeneration of cDNA probes dire ...Sus scrofaScience2391288-12911988--1--------1-4--------1--------------1-----------1--------1---------------
394135SanchezPurification, cDNA cloning, an ...Phaseolus vulgarisPlant Physiol.841143-11471987--1-----1-11-4-----1--11-------------1--------1-11-----1--11--------------
394136KralCloning a cDNA for Drosophila ...Drosophila melanogasterGene45131-1371986--1--------1-4-----4--1--------------1-----------1-----4--1---------------
394137Demnerova-Production of uricase by diffe ...Streptomyces aureofaciensBiotechnol. Lett.8577-5781986-----------1-1--------1--------------------------1--------1---------------
394138Van den Bosch-Immunogold localization of nod ...Glycine maxPlanta167425-4361986--------1--1-1--------1-----------------------1--1--------1---------------
394139Kralova-Extraction of uricase from Can ...Cyberlindnera jadiniiBiotechnol. Lett.899-1021986------1----1-1--1-----1--------------------1-----1---1----1---------------
394140SalasRole of uricase in the trigger ...Bacillus fastidiosusBiochem. J.229241-2491985------7-1--1-3-----2--1--------------------7--1--1-----2--1---------------
394141LucasUricase from soybean root nodu ...Glycine maxArch. Biochem. Biophys.226190-1971983------42--21-4--1--41-12----11-------------4-2--21---1-41-12----11--------
394142NishimuraPhysicochemical properties and ...Cyberlindnera jadiniiJ. Biochem.9141-481982-----151-211-3--1-----11-----------------1-5-1-211---1----11--------------
394143ChenProperties of two urate oxidas ...Cyberlindnera jadinii, Sus scrofaBiochim. Biophys. Acta660293-2981981-----------2-4-----2--2--------------------------2-----2--2---------------
394144OhePurification and properties of ...Streptomyces cyanogenusJ. Biochem.891769-17761981------611211-2--1---11111-2-2-1------------6-11211---1--11111-2-2-1-------
394145RainbirdPurification and some properti ...Vigna unguiculataBiochim. Biophys. Acta659132-1401981-----1202-121-3--1--11112--1-1------------1-20-2-121---1-11112--1-1---------
394146Machida-Purification and properties of ...Enterobacter cloacaeAgric. Biol. Chem.442811-28151980------611-11-1--1---1-1-112-113------------6-11-11---1--1-1-112-113-------
394147ConleyNon-classical inhibition of ur ...Aspergillus flavusBiochem. J.187733-7381980------3----1-3--------1--------------------3-----1--------1---------------
394148WangPurification and characterizat ...Neurospora crassaArch. Biochem. Biophys.201185-1931980------511-11-2--1-----11-------------------5-11-11---1----11--------------
394149BongaertsMechanism of uricase action ...Bacillus fastidiosusBiochim. Biophys. Acta567295-3081979-----------1-1--------1--------------------------1--------1---------------
394150BongaertsUricase of Bacillus fastidiosu ...Bacillus fastidiosusBiochim. Biophys. Acta527348-3581978------21-2211-2--1----1111-2-11-1------1----21--2211---1---1111-2-11--------
394152WatanabeA simple purification method f ...Rattus norvegicusAnal. Biochem.89343-3471978-----------1-2--1--2--11-------------------------1---1-2--11--------------
394153JohnsonStudies on the stability of im ...Sus scrofaBiotechnol. Bioeng.201085-10951978-----1-----1-1--------1------------------1-------1--------1---------------
394154AntonenkovOrganization of urate oxidase ...Rattus norvegicusFEBS Lett.88151-1541978--------1-11-1------1-11----------------------1-11------1-11--------------
394155TanakaProduction of uricase by Candi ...Candida tropicalisAppl. Environ. Microbiol.34342-3461977--------1--1-3--------1-----------------------1--1--------1---------------
394157SedorInhibition of uricase by subst ...Sus scrofaBiochem. Biophys. Res. Commun.75406-4131977-----------1-1-----1--1--------------------------1-----1--1---------------
394156HinbergPreparation and kinetic proper ...Sus scrofaBiotechnol. Bioeng.171435-14411975-----------1-1-----1--1-----222------------------1-----1--1-----222-------
394175ParishMitochondria and peroxisomes f ...Dictyostelium discoideumEur. J. Biochem.58523-5311975------1-3--1-2--------1-----1--------------1--3--1--------1-----1---------
394176Fitzpatrick-Resistance of urate oxidase to ...Aspergillus flavus, Bacillus fastidiosus, Cyberlindnera jadiniiBiochem. Soc. Trans.31253-12551975-----3-----3-3--------3------------------3-------3--------3---------------
394158Johnson-Characterization of immobilize ...Sus scrofaBiochem. Soc. Trans.21362-13631974-----------1-1-----1--1--------------------------1-----1--1---------------
394159PittsUricase, subunit composition a ...Sus scrofaBiochemistry13888-8921974-----2---111-1-----1--11-----------------2-----111-----1--11--------------
394177Theimer-Control of particulate urate o ...Phaseolus coccineusZ. Pflanzenphysiol.73360-3701974--------1--1-1-----1--1-----------------------1--1-----1--1---------------
394166TurnerEffect of copper on urate oxid ...Bos taurusEnzymologia4357-701972-----------1-1-----1--1---2----------------------1-----1--1---2-----------
394168Parish-Urate oxidase in peroxisomes f ...Zea maysPlanta104247-2511972--------1--1-1-----1--1-----------------------1--1-----1--1---------------
246662TheimerUricase and allantoinase in gl ...Arachis hypogaea, Carthamus tinctorius, Helianthus sp., Pinus monophylla, Ricinus communis, Yucca brevifolia, Zea maysPlant Physiol.47246-2511971------4-6--7-101----14--7-----1--------------4--6--7-1---14--7-----1---------
394169Itaya-Studies on urate oxidase of Ca ...Cyberlindnera jadiniiAgric. Biol. Chem.35813-8211971------6--211-1--------1--------------------6---211--------1---------------
394151MahlerA new bacterial uricase for ur ...Bacillus fastidiosusAnal. Biochem.3865-841970--------1--1-1--------1-----------------------1--1--------1---------------
394167ChristenUrate oxidase in primate phylo ...Oryctolagus cuniculus, Platyrrhini, Tupaia glisEur. J. Biochem.123-51970-----------3-3-----3--3--------------------------3-----3--3---------------
394164JamesEffects of treatment with dith ...Bos taurusEnzymologia37131-1521969-----1--1-11-1--1--1--1------------------1----1-11---1-1--1---------------
394165BentleyEffect of guanidine derivates ...Bos taurusEnzymologia37285-3131969------5----1-1--------1--------------------5-----1--------1---------------
394171TownsendPurification of urate oxidase; ...Rattus norvegicusArch. Biochem. Biophys.135166-1721969-----34----1-2--1--2211-----1------------3-4-----1---1-2211-----1---------
394172MullerUrate oxidase and its associat ...Acanthamoeba sp.Eur. J. Biochem.9424-4301969------6-2--1-2--------1-----1--------------6--2--1--------1-----1---------
394161TruscoeEffects of some nitrogenous ba ...Bos taurusEnzymologia34337-3431968------3----1-1-----1--1--------------------3-----1-----1--1---------------
394162Truscoe-Effect of borate on urate oxid ...Bos taurus, Sus scrofaEnzymologia34325-3361968-----5-----2-2--------2------------------5-------2--------2---------------
394163TruscoeEffect of phosphate on the act ...Bos taurusEnzymologia3519-301968------1----1-1-----1--1--------------------1-----1-----1--1---------------
394173NoseStudies on bacterial urate:oxy ...Arthrobacter pascensBiochim. Biophys. Acta15163-691968-----32--2-1-1--------1------------------3-2---2-1--------1---------------
394174ArimaStudies on bacterial urate:oxy ...Arthrobacter pascensBiochim. Biophys. Acta15154-621968------4--1-1-1--1----11-----1--------------4---1-1---1---11-----1---------
394160TruscoeEffect of detergents on extrac ...Bos taurusEnzymologia3319-321967-----------1-1--1--1--1--------------------------1---1-1--1---------------
394178Itaya-Studies on yeast uricase. Part ...Cyberlindnera jadiniiAgric. Biol. Chem.311256-12641967-----211---1-1--1---1-1-111-1-1----------2-1-1---1---1--1-1-111-1-1-------