Experimental evidence for a link among cupredoxins: red, blue, and purple copper transformations in nitrous oxide reductase

Savelieff, M.G.; Wilson, T.D.; Elias, Y.; Nilges, M.J.; Garner, D.K.; Lu, Y.; Proc. Natl. Acad. Sci. USA 105, 7919-7924 (2008)

 show all sequences of 1.7.2.4

Data extracted from this reference:

Cloned(Commentary)
Commentary Organism
expression as metal-free apo protein Paracoccus denitrificans
Metals/Ions
Metals/Ions Commentary Organism Structure
Cu2+ a copper enzyme with cupredoxin containing blue T1 copper and red T2 copper. Blue and red copper centers form initially before they are pH-dependently transformed into purple CuA center, lower pH resulting in fewer trapped T1 and T2 coppers and faster transition Paracoccus denitrificans
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Paracoccus denitrificans Q51705
-
-
Purification (Commentary)
Commentary Organism
recombinant metal-free apo protein Paracoccus denitrificans
Cofactor
Cofactor Commentary Organism Structure
cupredoxin a copper enzyme with cupredoxin containing blue T1 copper and red T2 copper. Blue and red copper centers form initially before they are pH-dependently transformed into purple CuA center, lower pH resulting in fewer trapped T1 and T2 coppers and faster transition. Structure overview Paracoccus denitrificans
-
Cloned(Commentary) (protein specific)
Commentary Organism
expression as metal-free apo protein Paracoccus denitrificans
Cofactor (protein specific)
Cofactor Commentary Organism Structure
cupredoxin a copper enzyme with cupredoxin containing blue T1 copper and red T2 copper. Blue and red copper centers form initially before they are pH-dependently transformed into purple CuA center, lower pH resulting in fewer trapped T1 and T2 coppers and faster transition. Structure overview Paracoccus denitrificans
-
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Cu2+ a copper enzyme with cupredoxin containing blue T1 copper and red T2 copper. Blue and red copper centers form initially before they are pH-dependently transformed into purple CuA center, lower pH resulting in fewer trapped T1 and T2 coppers and faster transition Paracoccus denitrificans
Purification (Commentary) (protein specific)
Commentary Organism
recombinant metal-free apo protein Paracoccus denitrificans

See also following references to EC number 1.7.2.4 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
700984SavelieffExperimental evidence for a li ...Paracoccus denitrificansProc. Natl. Acad. Sci. USA1057919-79242008--1------1---3--1--------------1-----11--------1-----1--------------------
698268FujitaInsights into the mechanism of ...Achromobacter cycloclastesInorg. Chem.46613-6152007-------1-1-1-3------1-2-----11---------------1-1-1------1-2-----11--------
698456GhoshSpectroscopic, computational, ...Achromobacter cycloclastes, Marinobacter hydrocarbonoclasticusJ. Am. Chem. Soc.1293955-39652007---------2---5----------2----------------------2------------2-------------
699384FujitaAnaerobic purification, charac ...Achromobacter cycloclastesJ. Inorg. Biochem.1011836-18442007--1----1-1-1-4--1---1-111---11-------1-------1-1-1---1--1-111---11--------
700303Bingham-Magnetic circular dichroism an ...Paracoccus pantotrophusMol. Phys.1052169-21762007---------1---1---------1-----------------------1-----------1--------------
671444HornNitrous oxide reductase genes ...Dechloromonas denitrificans, Flavobacterium denitrificans, Pseudomonas sp.Appl. Environ. Microbiol.721019-10262006-------------13-----1--3--------------------------------1--3---------------
671455Sameshima-SaitoSymbiotic Bradyrhizobium japon ...Bradyrhizobium japonicumAppl. Environ. Microbiol.722526-25322006----1--------3--------1-----------------1-----------------1---------------
671469HenryQuantitative detection of the ...Achromobacter cycloclastes, Alcaligenes faecalis, Bradyrhizobium japonicum, Ensifer adhaerens, Hyphomicrobium denitrificans, Pseudomonas denitrificans, Pseudomonas fluorescens, Sinorhizobium melilotiAppl. Environ. Microbiol.725181-51892006-------------13--------8-----------------------------------8---------------
672074TaubnerStructural studies of Apo NosL ...Achromobacter cycloclastesBiochemistry4512240-122522006--1------1---2--1--------------------1---------1-----1--------------------
674195GorelskyMechanism of N2O reduction by ...Pseudomonas sp.J. Am. Chem. Soc.128278-2902006---------1---2--------1------------------------1----------1---------------
675403ParaskevopoulosInsight into catalysis of nitr ...Achromobacter cycloclastesJ. Mol. Biol.36255-652006---1--1--1---4--1---1-11---------------1---1---1-----1--1-11--------------
660478MattilaHow does nitrous oxide reducta ...Paracoccus denitrificans, Rhodobacter sphaeroidesProteins59708-7222005--------12-1-5--------51----------------------12-1--------51--------------
673234RasmussenFormation of a cytochrome c-ni ...Paracoccus pantotrophusDalton Trans.20053501-35062005--------11---5--------31----------------------11----------31--------------
673622WunschNosX function connects to nitr ...Paracoccus denitrificansFEBS Lett.5794605-46092005----1--------2--1---3-1-----------------1------------1--3-1---------------
657567VelascoMolecular characterization of ...Bradyrhizobium japonicumAntonie Leeuwenhoek85229-2352004--------1--1-2--------11----------------------1--1--------11--------------
658979ChanReductively activated nitrous ...Achromobacter cycloclastesJ. Am. Chem. Soc.1263030-30312004---------1-1-1--1---1-3------------------------1-1---1--1-3---------------
394516HaltiaThe crystal structure of nitro ...Paracoccus denitrificansBiochem. J.36977-882003---1-----2-1-3--------21---------------1-------2-1--------21--------------
659085YamaguchiCharacterization of nitrous ox ...Hyphomicrobium denitrificansJ. Biochem.134853-8582003---------111-5--1---2-21----11-----------------111---1--2-21----11--------
659880AraiTranscriptional regulation of ...Pseudomonas aeruginosaMicrobiology14929-362003--1-----11-1-3--------11-------------1--------11-1--------11--------------
394517RasmussenMultiple forms of the catalyti ...Paracoccus pantotrophusBiochem. J.364807-8152002------1--1-1-5------5-2--------------------1---1-1------5-2---------------
394518RasmussenThe catalytic center in nitrou ...Paracoccus pantotrophus, Pseudomonas stutzeriBiochemistry3912753-127562000-2--1----2-2-3------2-2-------------2---1------2-2------2-2---------------
394519PrudencioPurification, Characterization ...Marinobacter hydrocarbonoclasticusBiochemistry393899-39072000---1-----111-3--1---2-11---------------1-------111---1--2-11--------------
394520BrownA novel type of catalytic copp ...Marinobacter hydrocarbonoclasticusNat. Struct. Biol.7191-1952000---1----1--1-4--------11---------------1------1--1--------11--------------
394521SatoAnaerobic purification and cha ...Rhodobacter sphaeroidesJ. Biochem.125864-8681999-----11----1-4--1---21211-1-1------------1-1-----1---1--21211-1-1---------
394522SabatyNitrite and nitrous oxide redu ...Rhodobacter sphaeroidesJ. Bacteriol.1816028-603219991----------1-4--------1------------1-------------1--------1---------------
394523FerrettiBiochemical characterization a ...Achromobacter xylosoxidansEur. J. Biochem.259651-6591999---------111-8--1---1-21-1---1-----------------111---1--1-21-1---1--------
394524SatoCharacterization of a multi-co ...Rhodobacter sphaeroidesJ. Biochem.12451-541998------3--111-4--1---1-21-------------------3---111---1--1-21--------------
394525FarrarCuA and CuZ are variants of th ...Pseudomonas stutzeriProc. Natl. Acad. Sci. USA959891-98961998--------11-1-3--------1-----------------------11-1--------1---------------
394526HoleCharacterization of the membra ...Thiobacillus denitrificansArch. Microbiol.16555-611996------1-1111-4------1-11-------------------1--1111------1-11--------------
394527DreuschMutation of the conserved Cys1 ...Pseudomonas stutzeriEur. J. Biochem.237447-4531996----1---11-1-2--------1-----------------1-----11-1--------1---------------
394528Zhang-The reaction of reduced cytoch ...Wolinella succinogenesBiochim. Biophys. Acta1142253-2611993---------1-1-1--------2------------------------1-1--------2---------------
394529BerksPurification and characterizat ...Paracoccus pantotrophusEur. J. Biochem.212467-4761993-----13--111-6--1---4-21----1---3--------1-33--111---1--4-21----1---------
665462SooHooPurification and characterizat ...Pseudomonas aeruginosaJ. Biol. Chem.2662203-22091991-------2-11--3--1---2-11---------------------2-11----1--2-11--------------
394530Jones-Denitrification in Flexibacter ...Solitalea canadensisCan. J. Microbiol.36430-4341990------2----1-1--------1--------------------2-----1--------1---------------
394531HulseIsolation of a high specific a ...Achromobacter cycloclastesBiochem. Biophys. Res. Commun.166729-7351990------1--1-1-3--------21-------------------1---1-1--------21--------------
394532Bonin-Effects of oxygen on each step ...Marinobacter hydrocarbonoclasticusCan. J. Microbiol.351061-10641989------1----1-1--------1--------------------1-----1--------1---------------
394533TeraguchiPurification and some characte ...Wolinella succinogenesJ. Biol. Chem.2641972-19791989-------2-311-3--1---1221---------------------2-311---1--1221--------------
394534ViebrockMolecular cloning, heterologou ...Pseudomonas stutzeriJ. Bacteriol.1704658-46681988--1--------1-5--------1--------------1-----------1--------1---------------
394535CoyneNitrous oxide reduction in nod ...Rhizobium sp.Appl. Environ. Microbiol.531168-11701987------1----1-5--------1--------------------1-----1--------1---------------
394536SnyderPurification and some characte ...Paracoccus denitrificansJ. Biol. Chem.2626515-65251987-----131-111-5--1---1-11-----------------1-3-1-111---1--1-11--------------
394538Michalski-Purification and characterizat ...Rhodobacter sphaeroidesBiochim. Biophys. Acta87250-601986---------311-1------1-2------------------------311------1-2---------------
394542AidaTemporary low oxygen condition ...Pseudomonas sp.Can. J. Microbiol.32543-5471986---------1-1-6--------1------------------------1-1--------1---------------
394539CoyleNitrous oxide reductase from d ...Pseudomonas stutzeriEur. J. Biochem.153459-4671985------1--121-3--1-----21--1----------------1---121---1----21--1-----------
394540McEwanNitrous oxide reduction by mem ...Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodopseudomonas palustris, Rhodospirillum rubrumJ. Bacteriol.164823-8301985------2-14-4-12--------4--------------------2--14-4--------4---------------
394537Kucera-Amperometric assay of activity ...Paracoccus denitrificansCollect. Czech. Chem. Commun.492709-27121984-----------1-1--------2-----1--------------------1--------2-----1---------
394541SnyderNitrous oxide reductase and th ...Paracoccus denitrificans, Pseudomonas denitrificans, Pseudomonas stutzeriBiochem. Biophys. Res. Commun.119588-5921984------3--333-4--------6--------------------3---333--------6---------------
394543Zumft-A novel kind of multi-copper p ...Pseudomonas stutzeriFEBS Lett.148107-1121982------1--111-1--------21-------------------1---111--------21--------------
394544Matsubara-Identification of a copper pro ...Pseudomonas fluorescensArch. Microbiol.132322-3281982----------11-1--------1-------------------------11--------1---------------
394545Kristjansson-Partial purification and chara ...Paracoccus denitrificansCurr. Microbiol.6247-2511981------3---11-1--1---1-3-----11-------------3----11---1--1-3-----11--------
394546KristjanssonFirst practical assay for solu ...Paracoccus denitrificansJ. Biol. Chem.255704-7071980------43---1-4--------2---------4----------443---1--------2---------------