Structural basis for the inactivation of Thermus thermophilus proline dehydrogenase by N-propargylglycine

White, T.A.; Johnson, W.H.; Whitman, C.P.; Tanner, J.J.; Biochemistry 47, 5573-5580 (2008)

 show all sequences of 1.5.99.8

Data extracted from this reference:

Cloned(Commentary)
Commentary Organism
-
 Thermus thermophilus
Crystallization (Commentary)
Crystallization Organism
sitting drop vapour diffusion method, with 100 mM imidazole buffer at pH 7, 100 mM MgCl2, 14% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol Thermus thermophilus
Inhibitors
Inhibitors Commentary Organism Structure
N-propargylglycine
-
 Thermus thermophilus
Localization
Localization Commentary Organism GeneOntology No. Textmining
mitochondrion
-
 Thermus thermophilus 5739
-
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
37980
-
 native enzyme, calculated from sequence of cDNA Thermus thermophilus
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Thermus thermophilus Q72IB8
-
-
Purification (Commentary)
Commentary Organism
-
 Thermus thermophilus
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-proline + 2,6-dichlorophenolindophenol + H2O
-
 685248 Thermus thermophilus (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
 ?
Cofactor
Cofactor Commentary Organism Structure
FAD
-
 Thermus thermophilus
Ki Value [mM]
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.2
-
 N-propargylglycine at 25°C Thermus thermophilus
Cloned(Commentary) (protein specific)
Commentary Organism
-
 Thermus thermophilus
Cofactor (protein specific)
Cofactor Commentary Organism Structure
FAD
-
 Thermus thermophilus
Crystallization (Commentary) (protein specific)
Crystallization Organism
sitting drop vapour diffusion method, with 100 mM imidazole buffer at pH 7, 100 mM MgCl2, 14% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol Thermus thermophilus
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
N-propargylglycine
-
 Thermus thermophilus
Ki Value [mM] (protein specific)
Ki Value [mM] Ki Value maximum [mM] Inhibitor Commentary Organism Structure
0.2
-
 N-propargylglycine at 25°C Thermus thermophilus
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
mitochondrion
-
 Thermus thermophilus 5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
37980
-
 native enzyme, calculated from sequence of cDNA Thermus thermophilus
Purification (Commentary) (protein specific)
Commentary Organism
-
 Thermus thermophilus
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
L-proline + 2,6-dichlorophenolindophenol + H2O
-
 685248 Thermus thermophilus (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
 ?

See also following references to EC number 1.5.99.8 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
710742SatomuraCrystallization and preliminar ...Aeropyrum pernixActa Crystallogr. Sect. F661508-15102010--11------1--2---------1-------------1-1--------1----------1--------------
711252SrivastavaThe structure of the proline u ...Escherichia coliBiochemistry49560-5692010--11--1------3-----------------------1-1---1------------------------------
711615FunckNon-redundant functions of two ...Arabidopsis thalianaBMC Plant Biol.10702010--------1----3-----3--------------------------1--------3------------2112--
696361OstranderA conserved active site tyrosi ...Escherichia coliBiochemistry48951-9592009--112-16-----4--1-----2----6---13----1112--136-------1----2----6----------
684911MaxwellIdentification of a p53-respon ...Homo sapiensBiochem. Biophys. Res. Commun.369308-3132008--1-----1----1--------1--------------1--------1-----------1---------------
685248WhiteStructural basis for the inact ...Thermus thermophilusBiochemistry475573-55802008--11--1-1-1--3--1-----1--------11----111---11-1-1----1----1---------------
689987Di RosaType I hyperprolinemia and pro ...Homo sapiensPsychiatr. Genet.1840-422008-1--15--------3-----1--1-------------1---15--------------1--1---------------
695364SchuermannThree crystal forms of the bif ...Bradyrhizobium japonicumActa Crystallogr. Sect. F64949-9532008--11---------3--1-----1--------------1-1-------------1----1---------------
695521PhangThe metabolism of proline, a s ...Homo sapiensAmino Acids35681-6902008-------------2------------------------------------------------------------
699706MitsubuchiInborn errors of proline metab ...Homo sapiensJ. Nutr.1382016S-2020S2008--------1----2-----3---1----------------------1--------3---1--------------
675953HuOverexpression of proline oxid ...Homo sapiensMol. Cell. Biochem.29585-9220071-1-----1----1-----2--1--------1---1-11-------1--------2--1---------------
686651MonaghanMechanistic aspects and redox ...Pyrococcus furiosusFEBS J.2742070-20872007--1-3--91-2--5--1-----41--19-113-----13-3----91-2----1----41--19-11-------
687234HuangDELTA1-pyrroline-5-carboxylic ...Bacillus subtilisJ. Agric. Food Chem.555097-51022007--1-----1-2--4--1---1-1-1-1-1--1-----11-------1-2----1--1-1-1-1-1---------
687550WhiteStructure and kinetics of mono ...Thermus thermophilusJ. Biol. Chem.28214316-143272007--11--431-1--4--1-----5---13---13----111---4331-1----1----5---13----------
689071KimApoptotic action of peroxisome ...Homo sapiensMol. Pharmacol.72674-68520071-------1----1-----4--1------------1----------1--------4--1---------------
689670RibaritsTwo tobacco proline dehydrogen ...Nicotiana tabacumPlanta2251313-13242007----------1--6-----8--1-------------------------2------16--2---------------
690036Kolodyazhnaya-Tobacco transformants expressi ...Nicotiana tabacumRuss. J. Genet.43825-8282007--1----------1-----1-----------------1-----------------1------------------
677108Kolodyazhnaya-Evaluation of salt tolerance i ...Nicotiana tabacumRuss. J. Genet.42212-2142006----1--------1--------------------------1---------------------------------
660326MillerResponsive modes of Medicago s ...Medicago sativaPlanta22270-792005--1----------3-----------------------1------------------------------------
671083WhiteCloning, purification and crys ...Thermus thermophilusActa Crystallogr. Sect. F61737-7392005---1---------4-------------------------1----------------------------------
671084MonaghanCrystallization and preliminar ...Pyrococcus furiosusActa Crystallogr. Sect. F61756-7582005--11---------1--1------1-------------1-1-------------1-----1--------------
671977ZhuExploring the proline-dependen ...Escherichia coliBiochemistry4412297-123062005----4--41----3---1----1----4------------4----41-----------1----4----------
673527KawakamiA second novel dye-linked L-pr ...Pyrococcus horikoshiiFEBS J.2724044-40542005-------2--2--6------2-22--1-1-13------3------2--2-------2-22--1-1-1-------
674471TsugeCrystal structure of a novel F ...Pyrococcus horikoshiiJ. Biol. Chem.28031045-310492005---1---------5--1------1-------3------31-------------1-----1--------------
658033ZhangStructures of the Escherichia ...Escherichia coliBiochemistry4312539-125482004--111-32--11-5--1-----1---12----3----1-11--332--11---1----1---12----------
658211BabanProbing a hydrogen bond pair a ...Escherichia coliBiochim. Biophys. Acta170149-592004--1-3--4-----3--------1----4---------1--3----4------------1----4----------
658668KawakamiGene and primary structures of ...Thermococcus profundusExtremophiles899-1082004--1----1-11--9--1---1-211-1-1-11-----11------1-11----1--1-211-1-1-1-------
659027LeeCharacterization of the Vibrio ...Vibrio vulnificusJ. Bacteriol.1853842-38522003-------------3------------------------------------------------------------
659987LeeStructure of the proline dehyd ...Escherichia coliNat. Struct. Biol.10109-1142003---1-------1-4--------11---------------1---------1--------11--------------
660194AbrahamLight-dependent induction of p ...Arabidopsis sp.Plant Mol. Biol.51363-3722003------2------3-----2-----------------------2-----------2------------------
392566ManiAltered levels of proline dehy ...Arabidopsis thalianaPlant Physiol.12873-832002----1---1----3--------11----------------1-----1-----------11--------------
392565NadaraiaCrystallization and preliminar ...Escherichia coliActa Crystallogr. Sect. D571925-19272001---1---------3--------1----------------1------------------1---------------
392570SakurabaPurification, characterization ...Thermococcus profundusAppl. Environ. Microbiol.671470-14752001-------1--1--3--1-----11--2---11------1------1--1----1----11--2---1-------
392572Chaudhary-Proline dehydrogenase activity ...Vigna radiataIndian J. Exp. Biol.371234-12401999----1--------1--------------------------1---------------------------------
392568TritschMechanism-based inhibition of ...Drosophila melanogaster, Glossina morsitans, Rattus norvegicusBiochim. Biophys. Acta120277-811993------511----7-----1--4---------1----------5111--------1--4---------------
392571BrownConformational change and memb ...Escherichia coliJ. Biol. Chem.2688972-89791993-----1-------2-----------------1------1--1--------------------------------
392569Deutch-Oxidation of L-thiazolidine-4- ...Escherichia coliJ. Gen. Microbiol.1381593-15981992------1------1--------2--------------------1--------------2---------------
392555WoodMembrane association of prolin ...Escherichia coliProc. Natl. Acad. Sci. USA84373-3771987--1-----2--1-3--------2--------------1--------2--1--------2---------------
392556GrahamProline dehydrogenase from Esc ...Escherichia coliJ. Biol. Chem.2592656-26611984------221--1-4--1-----3--------1------1----2-21--1---1----3---------------
392557MeilePurification and properties of ...Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar TyphimuriumEur. J. Biochem.12967-751982------41--12-2--11--2151----1--1------1----4-1--12---1--2151----1---------
392558ElthonProline oxidation in corn mito ...Zea maysPlant Physiol.70567-5721982--------2--1-2--------2-----1--1------1-------2--1--------2-----1---------
392559MommsenRespiratory and enzymatic prop ...Illex illecebrosusEur. J. Biochem.120345-3501981--------2--1-5-----1--2-----------------------2--1-----1--2---------------
392560ScarpullaMembrane-bound proline dehydro ...Escherichia coliJ. Biol. Chem.2535997-60011978------511-11-2--1---1-41----1--14-----1----5411-11---1--1-41----1---------
392561BalboniStudies on the inner mitochond ...Phormia reginaBiochim. Biophys. Acta462171-17619771-------2----3-----2--1------------1----------2--------2--1---------------
392562KramarSolubilization of proline dehy ...Rattus norvegicusHoppe-Seyler's Z. Physiol. Chem.3521267-12701971-------23--1-2--1--2--2-----1----------------23--1---1-2--2-----1---------
392563CrabtreeThe activities of proline dehy ...insectaBiochem. J.1171019-10211970-------12--1-1-----1--2----------------------12--1-----1--2---------------
392564HansfordThe control of the oxidation o ...Phormia reginaJ. Biol. Chem.245991-99419701------21----1-----1--1--------1---1--1------21--------1--1---------------