Purification, cDNA cloning and expression of 15-oxoprostaglandin 13-reductase from pig lung

Ensor, C.M.; Zhang, H.; Tai, H.H.; Biochem. J. 330, 103-108 (1998)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
expression in Escherichia coli	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.0077	-	15-ketoprostaglandin E1	-	Sus scrofa
0.0083	-	15-ketoprostaglandin E1	recombinant enzyme	Sus scrofa
0.01	-	leukotriene B4	recombinant enzyme	Sus scrofa
0.015	-	NADPH	-	Sus scrofa
0.016	-	NADPH	recombinant enzyme	Sus scrofa
0.0182	-	15-Ketoprostaglandin E2	recombinant enzyme	Sus scrofa
0.019	-	15-Ketoprostaglandin E2	-	Sus scrofa
0.088	0.094	NADH	-	Bos taurus
0.153	-	NADH	-	Sus scrofa
0.16	-	NADH	recombinant enzyme	Sus scrofa

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Bos taurus	-	-	-
Sus scrofa	-	-	-

Purification (Commentary)

Commentary	Organism
native and recombinant enzyme	Sus scrofa

Source Tissue

Source Tissue	Commentary	Organism	Textmining
lung	-	Bos taurus	-
lung	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
2.56	-	-	Sus scrofa
2.6	-	recombinant enzyme expressed in Escherichia coli	Sus scrofa

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
(5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH	trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity	390827	Sus scrofa	?	-	-	-	?
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H	-	390827	Sus scrofa	(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+	-	390827	Sus scrofa	ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H	-	390827	Bos taurus	(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+	-	390827	Bos taurus	ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H	-	390827	Sus scrofa	11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+	-	-	-	?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H	-	390827	Bos taurus	11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+	-	390827	Bos taurus	?
15-ketoprostaglandin E1 + NADPH	-	390827	Sus scrofa	?	-	-	-	-
leukotriene B4 + NADPH	-	390827	Sus scrofa	?	-	-	-	-

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.517	-	15-ketoprostaglandin E1	cofactor NADH	Sus scrofa
1.52	-	15-ketoprostaglandin E1	cofactor NADPH	Sus scrofa

Cofactor

Cofactor	Commentary	Organism	Structure
NAD(P)H	-	Bos taurus
NAD(P)H	3fold higher activity with NADPH compared to NADH	Sus scrofa

Cloned(Commentary) (protein specific)

Commentary	Organism
expression in Escherichia coli	Sus scrofa

Cofactor (protein specific)

Cofactor	Commentary	Organism	Structure
NAD(P)H	-	Bos taurus
NAD(P)H	3fold higher activity with NADPH compared to NADH	Sus scrofa

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.0077	-	15-ketoprostaglandin E1	-	Sus scrofa
0.0083	-	15-ketoprostaglandin E1	recombinant enzyme	Sus scrofa
0.01	-	leukotriene B4	recombinant enzyme	Sus scrofa
0.015	-	NADPH	-	Sus scrofa
0.016	-	NADPH	recombinant enzyme	Sus scrofa
0.0182	-	15-Ketoprostaglandin E2	recombinant enzyme	Sus scrofa
0.019	-	15-Ketoprostaglandin E2	-	Sus scrofa
0.088	0.094	NADH	-	Bos taurus
0.153	-	NADH	-	Sus scrofa
0.16	-	NADH	recombinant enzyme	Sus scrofa

Purification (Commentary) (protein specific)

Commentary	Organism
native and recombinant enzyme	Sus scrofa

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
lung	-	Bos taurus	-
lung	-	Sus scrofa	-

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
2.56	-	-	Sus scrofa
2.6	-	recombinant enzyme expressed in Escherichia coli	Sus scrofa

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
(5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH	trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity	390827	Sus scrofa	?	-	-	-	?
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H	-	390827	Sus scrofa	(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+	-	390827	Sus scrofa	ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H	-	390827	Bos taurus	(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+	-	390827	Bos taurus	ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H	-	390827	Sus scrofa	11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+	-	-	-	?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H	-	390827	Bos taurus	11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+	-	390827	Bos taurus	?
15-ketoprostaglandin E1 + NADPH	-	390827	Sus scrofa	?	-	-	-	-
leukotriene B4 + NADPH	-	390827	Sus scrofa	?	-	-	-	-

Turnover Number [1/s] (protein specific)

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.517	-	15-ketoprostaglandin E1	cofactor NADH	Sus scrofa
1.52	-	15-ketoprostaglandin E1	cofactor NADPH	Sus scrofa

See also following references to EC number 1.3.1.48 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
699958	Zhao	-	Restoration of leukotriene B(4 ...	unidentified adenovirus	Lung Cancer	68	161-169	2010	-	-	1	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	4	1	-	1	-	1	-	-	-	1	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	4	1	-	1	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-
690204	Itoh		Leukotriene B4 12-hydroxydehyd ...	Rattus norvegicus	Xenobiotica	38	249-263	2008	-	-	1	-	-	-	6	9	1	-	-	-	-	1	-	-	1	-	-	1	-	-	11	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	1	-	-	-	-	6	-	9	1	-	-	-	-	-	-	1	-	1	-	-	11	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
687562	Chou		Identification of a novel pros ...	Mus musculus	J. Biol. Chem.	282	18162-18172	2007	-	-	1	-	-	-	-	5	-	-	-	-	-	2	-	-	1	-	-	2	4	-	5	-	-	-	-	5	-	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	5	-	-	-	-	-	-	-	1	-	2	4	-	5	-	-	-	-	5	-	-	-	-	-	-	-	-	-	-
674369	Hori		Crystal structure of anti-conf ...	Cavia porcellus	J. Biochem.	140	457-466	2006	-	-	-	1	-	-	1	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
656219	Hori		Structural basis of leukotrien ...	Cavia porcellus	J. Biol. Chem.	279	22615-22623	2004	-	-	-	1	-	-	-	-	-	-	-	1	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
390829	Yamamoto		Immunohistochemical localizati ...	Cavia porcellus	Eur. J. Biochem.	268	6105-6113	2001	-	-	1	-	-	-	-	2	-	-	-	-	-	9	-	-	1	-	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	1	-	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
390830	Dick		Antioxidative function and sub ...	Rattus norvegicus	J. Biol. Chem.	276	40803-40810	2001	-	-	1	-	-	-	-	20	-	-	-	-	-	4	-	-	1	-	-	-	-	-	20	-	-	-	-	20	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	20	-	-	-	-	-	-	-	1	-	-	-	-	20	-	-	-	-	20	-	-	-	-	-	-	-	-	-	-
390828	Clish		Oxidoreductases in lipoxin A4 ...	Sus scrofa	J. Biol. Chem.	275	25372-25380	2000	-	-	1	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
390827	Ensor	-	Purification, cDNA cloning and ...	Bos taurus, Sus scrofa	Biochem. J.	330	103-108	1998	-	-	1	-	-	-	-	10	-	-	-	-	-	2	-	-	1	-	-	2	2	-	7	-	-	-	-	2	-	-	-	2	-	-	-	-	-	1	2	-	-	-	-	-	-	10	-	-	-	-	-	-	-	1	-	2	2	-	7	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-
390825	Nikaido		15-Ketoprostaglandin delta 13- ...	Bos taurus	Curr. Eye Res.	12	17-22.	1993	-	-	-	-	-	-	4	-	1	-	1	-	-	1	-	-	1	-	-	4	6	2	1	1	-	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	4	-	-	1	-	1	-	-	-	-	1	-	4	6	2	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
390826	Kitamura		Purification of a 15-ketoprost ...	Rattus norvegicus	Biochem. Mol. Biol. Int.	30	839-847	1993	-	-	-	-	-	-	4	1	-	-	1	-	-	1	-	-	1	-	-	10	1	-	22	1	-	-	-	-	-	1	-	1	-	-	-	-	-	-	1	-	-	-	-	4	-	1	-	-	1	-	-	-	-	1	-	10	1	-	22	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-
390820	Hansen		Purification and assay of 15-k ...	Bos taurus, Gallus gallus, Homo sapiens	Methods Enzymol.	86	156-167	1982	-	-	-	-	-	-	3	7	-	-	2	6	-	3	-	-	1	-	-	3	1	2	15	-	1	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	3	-	7	-	-	2	6	-	-	-	1	-	3	1	2	15	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-
390821	Jarabak		Isolation and properties of a ...	Homo sapiens	Methods Enzymol.	86	163-167	1982	-	-	-	-	-	-	3	7	-	-	-	-	-	1	-	-	1	-	-	1	1	2	7	-	1	-	-	-	-	-	-	3	2	-	-	-	-	-	3	-	-	-	-	3	2	7	-	-	-	-	-	-	-	1	-	1	1	2	7	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-
390823	Hansen		Purification and characterizat ...	Bos taurus	Biochim. Biophys. Acta	574	136-145	1979	-	-	-	-	-	-	2	2	-	-	1	-	-	1	-	-	1	-	-	1	1	1	2	-	1	-	-	1	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	2	-	2	-	-	1	-	-	-	-	1	-	1	1	1	2	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-
390824	Westbrook		15-Ketoprostaglandin delta13 r ...	Homo sapiens	Arch. Biochem. Biophys.	185	429-442	1978	-	-	-	-	-	-	5	7	-	-	1	-	-	1	-	-	1	-	-	1	1	1	8	1	1	-	-	-	-	-	-	2	3	-	-	-	-	-	2	-	-	-	-	5	3	7	-	-	1	-	-	-	-	1	-	1	1	1	8	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-
390822	Westbrook		Purification and partial chara ...	Homo sapiens	Biochem. Biophys. Res. Commun.	66	541-546	1975	-	-	-	-	-	-	-	2	-	-	-	-	-	2	-	-	1	-	-	2	1	-	3	-	1	-	-	-	1	1	-	2	-	-	-	-	-	-	2	-	-	-	-	-	-	2	-	-	-	-	-	-	-	1	-	2	1	-	3	-	1	-	-	-	1	1	-	-	-	-	-	-	-	-