A functional cytochrome P450 lanosterol 14 alpha-demethylase CYP51 enzyme in the acrosome: transport through the Golgi and synthesis of meiosis-activating sterols

Cotman, M.; Jezek, D.; Fon Tacer, K.; Frangez, R.; Rozman, D.; Endocrinology 145, 1419-1426 (2004)

 show all sequences of 1.14.13.70

Data extracted from this reference:

Localization
Localization Commentary Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Bos taurus 5783
-
endoplasmic reticulum present during all phases of acrosome development Mus musculus 5783
-
endoplasmic reticulum
-
 Ovis aries 5783
-
Golgi apparatus
-
 Bos taurus 5794
-
Golgi apparatus present during all phases of acrosome development, in liver, no detection in trans-Golgi Mus musculus 5794
-
Golgi apparatus
-
 Ovis aries 5794
-
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Bos taurus
-
-
-
Mus musculus
-
-
-
Ovis aries
-
 ram
-
Posttranslational Modification
Posttranslational Modification Commentary Organism
glycoprotein
-
 Bos taurus
glycoprotein
-
 Mus musculus
glycoprotein
-
 Ovis aries
Source Tissue
Source Tissue Commentary Organism Textmining
germ cell male Bos taurus
-
germ cell male Mus musculus
-
germ cell male Ovis aries
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
lanosterol + O2 + NADPH
-
 658561 Mus musculus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
lanosterol + O2 + NADPH
-
 658561 Bos taurus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
lanosterol + O2 + NADPH
-
 658561 Ovis aries 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
Subunits
Subunits Commentary Organism
? x * 50000, SDS-PAGE Bos taurus
? x * 53000, SDS-PAGE Mus musculus
? x * 50000, SDS-PAGE Ovis aries
Cofactor
Cofactor Commentary Organism Structure
cytochrome P450
-
 Bos taurus
-
cytochrome P450
-
 Mus musculus
-
cytochrome P450
-
 Ovis aries
-
Cofactor (protein specific)
Cofactor Commentary Organism Structure
cytochrome P450
-
 Bos taurus
-
cytochrome P450
-
 Mus musculus
-
cytochrome P450
-
 Ovis aries
-
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Bos taurus 5783
-
endoplasmic reticulum present during all phases of acrosome development Mus musculus 5783
-
endoplasmic reticulum
-
 Ovis aries 5783
-
Golgi apparatus
-
 Bos taurus 5794
-
Golgi apparatus present during all phases of acrosome development, in liver, no detection in trans-Golgi Mus musculus 5794
-
Golgi apparatus
-
 Ovis aries 5794
-
Posttranslational Modification (protein specific)
Posttranslational Modification Commentary Organism
glycoprotein
-
 Bos taurus
glycoprotein
-
 Mus musculus
glycoprotein
-
 Ovis aries
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
germ cell male Bos taurus
-
germ cell male Mus musculus
-
germ cell male Ovis aries
-
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
lanosterol + O2 + NADPH
-
 658561 Mus musculus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
lanosterol + O2 + NADPH
-
 658561 Bos taurus 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
lanosterol + O2 + NADPH
-
 658561 Ovis aries 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + NADP+ + H2O
-
-
-
 ?
Subunits (protein specific)
Subunits Commentary Organism
? x * 50000, SDS-PAGE Bos taurus
? x * 53000, SDS-PAGE Mus musculus
? x * 50000, SDS-PAGE Ovis aries

See also following references to EC number 1.14.13.70 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
710892WarrilowExpression, purification, and ...Aspergillus fumigatusAntimicrob. Agents Chemother.544225-42342010--1--1-511---3--1-----3--------1-----11--1---511-----1----3----------11---
710895MartelComplementation of a Saccharom ...Aspergillus fumigatusAntimicrob. Agents Chemother.544920-49232010--1----------4-----------------------1------------------------------------
710939CoolsHeterologous expression of mut ...Mycosphaerella graminicolaAppl. Environ. Microbiol.762866-28722010--1-6---1----7-----------------------1--6-----1---------------------------
711171Petushkova-A fluorometric method for dete ...Mycobacterium tuberculosisBiochemistry (Moscow) Suppl. B4104-1062010------31---1-1--------2-1------------------3-1---1--------2-1-------------
712223LeeCloning and characterization o ...Taiwanofungus camphoratusJ. Agric. Food Chem.584800-48072010--1---1------4-----1--111---1----1---1-----1-----------1--111---1--11111--
712461LepeshevaStructural insights into inhib ...Trypanosoma cruziJ. Biol. Chem.28525582-255902010--11--3------4--1--------------------1-1---3---------1--------------------
701593Maurice-Virtual high screening through ...Mycobacterium tuberculosisAfr. J. Biotechnol.83072-30782009------9------1-----------------------------9------------------------------
701738ShengThree-dimensional model of lan ...Cryptococcus neoformansAntimicrob. Agents Chemother.533487-3495200911--1-6--1---4--------1------------11---1--6---1----------1---------------
702515KitahamaThe construction and character ...Saccharomyces cerevisiaeBiol. Pharm. Bull.32558-5632009--1---13-----5--1-----1--------1-----11----1-3-------1----1---------------
702576MingOrganocatalytic synthesis and ...Penicillium digitatumBioorg. Med. Chem. Lett.193938-39402009--1---20--1---1--1--------------1-----11----20---1-----1--------------------
703550ChaiDesign, synthesis, and biologi ...Aspergillus fumigatus, Candida albicans, Candida parapsilosis, Candida tropicalis, Cryptococcus neoformans, Microsporum gypseum, Pichia kudriavzevii, Trichophyton rubrumEur. J. Med. Chem.441913-19202009------182------13-----------------------------182------------------------------
705030KonkleIndomethacin amides as a novel ...Trypanosoma cruziJ. Med. Chem.522846-28532009--1---19--1---4--1--2--2--------1-----11----19---1-----1-2--2---------------
705342SenMolecular oxygen activation an ...Mycobacterium tuberculosisJ. Phys. Chem. B1138170-81822009---------1---3--------2--------1------1--------1----------2----------11---
706082YangExpression and homology modell ...Magnaporthe griseaPest Manag. Sci.65260-2652009-11---5--11--5-----------------1----111----5---11--------------------11---
684580LuoThe cytochrome P450 lanosterol ...Monilinia fructicolaAppl. Environ. Microbiol.74359-36620081-1--------1-3-----1--1------------1-1-----------1-----1--1---------------
684925SeliskarExpression of microsomal lanos ...Bos taurusBiochem. Biophys. Res. Commun.371855-8592008--1-1-1-4221-5--1--21-11----1--2-----12-1--1--4221---1-21-11----1---------
685534KorosecNovel cholesterol biosynthesis ...Homo sapiensBioorg. Med. Chem.16209-2212008-1----20-11-3-2-----1--4--------2----1-2----20--11-3-----1--4---------------
687710EddineX-ray structure of 4,4-dihydro ...Mycobacterium tuberculosisJ. Biol. Chem.28315152-151592008-1-1--5--2-4-5--------511---1--2----1-21---5---2-4--------511---1---------
688999Ning3,5-cyclic adenosine monophosp ...Mus musculusMol. Endocrinol.221682-169420081-----2111-2-3-----4--2--------2---1--2----2-111-2-----4--2---------------
701584BucknerSterol 14-demethylase inhibito ...Candida albicans, Homo sapiens, Mycobacterium tuberculosis, Trypanosoma brucei, Trypanosoma cruziAdv. Exp. Med. Biol.62561-802008------18--1---9--1--2--2--------2--29---2---2918---1-----1-2--2---------1--1--
701727ParkerDifferential azole antifungal ...Homo sapiens, Saccharomyces cerevisiaeAntimicrob. Agents Chemother.523597-36032008-11---16-2----5--------2-------------11-----16--2-----------2---------------
702509XuSilencing of mouse hepatic lan ...Mus musculusBiol. Pharm. Bull.311182-11912008------1------4-----5-----------------------1-----------5-------------11---
704035Krishnan-NatesanMolecular characterisation of ...Aspergillus flavusInt. J. Antimicrob. Agents32519-5242008--1-5-5------5-----------------------1--5--5------------------------------
705462WarrilowExpression and characterizatio ...Phanerochaete chrysosporiumLipids431143-11532008--1---5---1--4--1----1-1-------1-1---11----5----1----1---1-1-------1-11---
706694SongExpression and regulation of l ...Mus musculusReprod. Fertil. Dev.20964-97220082------------4-----4---------------2-------------------4------------1111--
672414LepeshevaSterol 14alpha-demethylase cyt ...Candida albicans, Homo sapiens, Methylococcus capsulatus, Mycobacterium smegmatis, Mycobacterium tuberculosis, Rattus norvegicus, Saccharomyces cerevisiae, Sorghum bicolor, Trypanosoma brucei, Trypanosoma cruzi, Ustilago maydisBiochim. Biophys. Acta1770467-4772007--11---29-11--22-12------11-6411-------12-----1112----29--11--22------11-6411--------------
673445SunSynthesis of novel triazole de ...Aspergillus fumigatus, Candida albicans, Candida parapsilosis, Candida tropicalis, Cryptococcus neoformans, Fonsecaea compacta, Microsporum gypseum, Trichophyton rubrumEur. J. Med. Chem.201-82007------256------16-----------------------------256------------------------------
684486PodustSmall-molecule scaffolds for C ...Mycobacterium tuberculosisAntimicrob. Agents Chemother.513915-39232007-2-11-3-11-1-3--------1--------1----2-111--3--11-1--------1---------------
685303ShumyantsevaElectrochemical reduction of s ...Mycobacterium tuberculosisBiochemistry (Moscow)72658-6632007----1-1--1-1-4--------1--------2------2-1--1---1-1--------1---------------
685453ChenIdentification of Y118 amino a ...Candida albicansBiol. Pharm. Bull.301246-12532007--1-3-1-11---4----------1---1--2-----12-3--1--11------------1---1---------
686798ZhaoExpression and homology modeli ...Penicillium digitatumFEMS Microbiol. Lett.27737-432007-11-1-1-11-1-5--1-----121---1--3----113-1--1--11-1---1----121---1---------
689083FraaijeA novel substitution 1381V in ...Mycosphaerella graminicolaMol. Plant Pathol.8245-2542007----3-4------7--------------------------3--4------------------------------
671268PietilaCloning and characterization o ...Mycobacterium avium subsp. aviumAm. J. Respir. Cell Mol. Biol.35236-2422006-11---5--1-2-6--1-----2-1---1--2----112----5---1-2---1----2-1---1---------
671942CoolsImpact of changes in the targe ...Blumeria graminis, Candida albicans, Mycosphaerella graminicola, Oculimacula acuformis, Oculimacula yallundaeBiochem. Soc. Trans.341219-12222006-5--7-13------8--------1-------------5---7--13--------------1---------------
672195McLeanBiophysical characterization o ...Mycobacterium tuberculosisBiochemistry458427-84432006--1--171-1---4--11----31--1-1--2-----12--1-7-1-1-----1----31--1-1---------
672607ZhuDesign, synthesis, and antifun ...Arthroderma otae, Aspergillus fumigatus, Candida albicans, Candida parapsilosis, Cryptococcus neoformans, Trichophyton rubrumBioorg. Med. Chem. Lett.165285-52892006------48----6-6---6----1266------6------6----48-----6--------1266-------------
673147Kojima-Cloning of chicken lanosterol ...Gallus gallusComp. Biochem. Physiol. A145A383-3892006--1----------2-----13-----------------2-----------------26------------------
674700LepeshevaCYP51 from Trypanosoma cruzi: ...Trypanosoma cruziJ. Biol. Chem.2813577-35852006-11-1-3-11-5-6--11--1-6--------1----111-1--3--11-5---1--1-6---------------
677216TroeskenComparison of lanosterol-14 al ...Candida albicans, Homo sapiensToxicology22824-322006--1---442-1-3-7------2-3-2---2--2--42--12---4244-2-1-3------2-3-2---2---------
658808WyandSequence variation in the CYP5 ...Blumeria graminisFungal Genet. Biol.42726-7352005--1-1-2------5-----------------------1--1--2------------------------------
659097KudoEffects of Y132H and F145L sub ...Candida albicansJ. Biochem.137625-6322005-1--2-4------3-----------------1----1-1-2--4------------------------------
659513MatsuuraStructural diversities of acti ...Homo sapiens, Mycobacterium tuberculosisJ. Biol. Chem.2809088-90962005------8------5--------2--------2------2----8--------------2---------------
671363MelladoTargeted gene disruption of th ...Aspergillus fumigatusAntimicrob. Agents Chemother.492536-25382005--1-1-7------3-----------------------1--1--7------------------------------
671727WatermanSterol 14alpha-demethylase, an ...Acanthamoeba polyphaga, Homo sapiens, Methylococcus capsulatus, Mycobacterium tuberculosis, Trypanosoma bruceiBiochem. Biophys. Res. Commun.338418-42220051-21----5--11-7-----6--12--------6---1-261------5--11-----6--12---------------
672337OttFlux of sterol intermediates i ...Saccharomyces cerevisiaeBiochim. Biophys. Acta1735111-1182005--1-1---3--1-3--------2--------1-----11-1-----3--1--------2---------------
673347FinkCholesterogenic lanosterol 14a ...Homo sapiensEndocrinology1465321-533120051-1--------1-2--------2--------1---1-11----------1--------2---------------
675865HankinsUpregulation of sterol C14-dem ...Trypanosoma cruziMol. Biochem. Parasitol.14468-7520051-1--------1-3--1-----1--------1---1-11----------1---1----1---------------
676589O'BrienLipid signaling in plants. Clo ...Solanum chacoensePlant Physiol.139734-7492005--1-----1--2-6-----4--31-------1-1---11-------1--2-----4--31-------1------
658561CotmanA functional cytochrome P450 l ...Bos taurus, Mus musculus, Ovis ariesEndocrinology1451419-14262004--------7----5-3---4--33-------3------3-------7-----3--4--33--------------
659531ShengHomology modeling of lanostero ...Aspergillus fumigatus, Candida albicansJ. Biomol. Struct. Dyn.2291-992004---2---------6-------------------------2----------------------------------
675115GinerSterol composition of Pneumocy ...Pneumocystis jiroveciiJ. Eukaryot. Microbiol.51634-6432004----1------1-5------1-1-----------------1--------1------1-1---------------
657522MoralesCharacterization of a lanoster ...Pneumocystis cariniiAm. J. Respir. Cell Mol. Biol.29232-2382003--1-2--------6---------1-------------1--2------------------1--------------
659601BurgerVirus-induced silencing of ste ...Nicotiana benthamianaJ. Exp. Bot.541675-16832003-1-----------6--------1-------------1---------------------1---------------
659901BucknerCloning and analysis of Trypan ...Trypanosoma cruziMol. Biochem. Parasitol.13275-812003--1-1--------6--------11-------1-----11-1-----------------11--------------
657808LambSterol 14alpha-demethylase act ...Streptomyces coelicolorBiochem. J.364555-5622002--1-1--------5--1-----2--------1-----11-1------------1----2---------------
659189JacksonA novel sterol 14alpha-demethy ...Methylococcus capsulatusJ. Biol. Chem.27746959-469652002---------11--5--------11-------1------1--------11---------11--------------
659942RozmanLanosterol 14alpha-demethylase ...Mus musculusMol. Cell. Endocrinol.187179-1872002-----------1-4-----3--1--------------------------1-----3--1---------------
285344BakCloning and expression in Esch ...Arabidopsis thaliana, Candida albicans, Candida glabrata, Candida tropicalis, Homo sapiens, Manihot esculenta, no activity in Escherichia coli, Penicillium italicum, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Sinapis alba, Sorghum bicolor, Ustilago maydis, Zea maysPlant J.11191-2011997-111---3-7--38-20--1--2--75-3---1--42----11142----3--7--38---1-2--75-3---1---------
285349AoyamaLanosterol 14-demethylase acti ...Rattus norvegicusJ. Biochem.120982-98619961-----2-3--5-3-----31-6-1---1--3---1--3----2--3--5-----31-6-1---1---------
285350KahnIsolation and reconstitution o ...Embryophyta, Sorghum bicolorJ. Biol. Chem.27132944-329501996-3----1-3-19-7--1--1-1191-------6----3-6----1--3-19---1-1-1191--------------
285352Van NistelrooyIsolation and molecular charac ...Candida albicans, Candida tropicalis, Penicillium italicum, Saccharomyces cerevisiaeMol. Gen. Genet.250725-7331996--1---1---11-10--------8--------8-----18----1----11--------8---------------
285354AoyamaSterol 14-demethylase P450 (P4 ...Candida albicans, Candida tropicalis, Embryophyta, Homo sapiens, Penicillium italicum, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Ustilago maydisJ. Biochem.119926-9331996--2-----3-229-12--1--2--33-1--1---27-----227-------3-229---1-2--33-1--1----------
285353SloaneCloning and functional express ...Rattus norvegicusGene161243-2481995-21---1---14-4--11-1--4--------2--1-212---11----14---1-1--4---------------
285348AoyamaOccurrence of a P450 showing h ...Candida albicans, Candida tropicalis, Embryophyta, Rattus norvegicus, Saccharomyces cerevisiaeBiochem. Biophys. Res. Commun.2011320-13261994--1--------5-8--1--32-13--------15-----115----------5---1-32-13---------------
285346AoyamaThe 4beta-methyl group of subs ...Embryophyta, Saccharomyces cerevisiaeBiochem. Biophys. Res. Commun.1831266-127219921-----55---11-4--1---1-42-1------6---1--6----5-5---11---1--1-42-1-------------
285345AoyamaDifferent substrate specificit ...Rattus norvegicus, Saccharomyces cerevisiaeBiochem. Biophys. Res. Commun.1781064-10711991-5----523--21-5--2--12-32-2------6----5-6----5-23--21---2-12-32-2-------------
285355TatonProperties and structural requ ...Zea maysBiochem. J.277483-4921991-3---1-34--6-2---1-1--21-1---11-3----3-3--1---34--6-----1--21-1---11--------
285347AoyamaRole of the 8-double bond of l ...Saccharomyces cerevisiaeBiochim. Biophys. Acta1001196-20019894------4---6-1--11--1-14-1---1--3---4--3------4---6---1--1-14-1---1---------
285351AoyamaYeast cytochrome P-450 catalyz ...Saccharomyces cerevisiaeJ. Biol. Chem.2591661-166619841-----611--5-1--11----6-1---1--3---1--3----6-11--5---1----6-1---1---------