Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole

Schultz, L.W.; Liu, L.; Cegielski, M.; Hastings, J.W.; Proc. Natl. Acad. Sci. USA 102, 1378-1383 (2005)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
histidine-tagged Lingulodinium polyedrum luciferase domain 3 is expressed in Escherichia coli BL21(DE3) cells	Lingulodinium polyedrum

Crystallization (Commentary)

Crystallization	Organism
Lingulodinium polyedrum luciferase domain 3, vapor diffusion method, using 18-20% (w/v) polyethylene glycol 2000 methyl ether and 100 mM (2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (pH 8.0)	Lingulodinium polyedrum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
More	Lingulodinium polyedrum	each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light	?	-	-	-

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Lingulodinium polyedrum	O77206	-	-

Purification (Commentary)

Commentary	Organism
nickel metal chelate column chromatography	Lingulodinium polyedrum

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
dinoflagellate luciferin + O2	-	713391	Lingulodinium polyedrum	oxidized dinoflagellate luciferin + H2O + hv	-	-	-	?
More	each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light	713391	Lingulodinium polyedrum	?	-	-	-	-

Cloned(Commentary) (protein specific)

Commentary	Organism
histidine-tagged Lingulodinium polyedrum luciferase domain 3 is expressed in Escherichia coli BL21(DE3) cells	Lingulodinium polyedrum

Crystallization (Commentary) (protein specific)

Crystallization	Organism
Lingulodinium polyedrum luciferase domain 3, vapor diffusion method, using 18-20% (w/v) polyethylene glycol 2000 methyl ether and 100 mM (2-hydroxyethyl)piperazine-N-(3-propanesulfonic acid) (pH 8.0)	Lingulodinium polyedrum

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
More	Lingulodinium polyedrum	each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light	?	-	-	-

Purification (Commentary) (protein specific)

Commentary	Organism
nickel metal chelate column chromatography	Lingulodinium polyedrum

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
dinoflagellate luciferin + O2	-	713391	Lingulodinium polyedrum	oxidized dinoflagellate luciferin + H2O + hv	-	-	-	?
More	each of the three domains of Lingulodinium polyedrum luciferase encodes an active luciferase that catalyzes the oxidation of a chlorophyll-derived open tetrapyrrole (dinoflagellate luciferin) to produce blue light	713391	Lingulodinium polyedrum	?	-	-	-	-

See also following references to EC number 1.13.12.18 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
713206	Suzuki-Ogoh		C-terminal region of the activ ...	Lingulodinium polyedrum	Photochem. Photobiol. Sci.	7	208-211	2008	-	-	1	-	1	-	-	-	-	-	-	1	-	3	-	-	1	-	-	-	-	-	2	1	-	-	-	-	2	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	2	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-
713393	Liu		Two different domains of the l ...	Noctiluca scintillans	Proc. Natl. Acad. Sci. USA	104	696-701	2007	-	-	1	-	-	-	-	-	-	-	1	-	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
712101	Suzuki		A new additional reporter enzy ...	Pyrocystis lunula	Gene	344	61-66	2005	-	1	1	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
713391	Schultz		Crystal structure of a pH-regu ...	Lingulodinium polyedrum	Proc. Natl. Acad. Sci. USA	102	1378-1383	2005	-	-	1	1	-	-	-	-	-	-	-	1	-	1	-	-	1	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
713390	Liu		Molecular evolution of dinofla ...	Lingulodinium polyedrum	Proc. Natl. Acad. Sci. USA	101	16555-16560	2004	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
710709	Liu		Characterization and crystalli ...	Lingulodinium polyedrum	Acta Crystallogr. Sect. D	59	761-764	2003	-	-	1	1	-	-	-	-	1	-	1	1	-	4	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	1	-	1	1	-	-	-	1	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
711192	Li		N-terminal intramolecularly co ...	Lingulodinium polyedrum	Biochemistry	40	1844-1849	2001	-	-	1	-	1	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	1	-	1	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-
712940	Li		Gonyaulax luciferase: gene str ...	Lingulodinium polyedrum	Methods Enzymol.	305	249-258	2000	-	-	1	-	-	-	-	-	1	-	3	-	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	3	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
712941	Morse		Dinoflagellate luciferin-bindi ...	Lingulodinium polyedrum	Methods Enzymol.	305	258-276	2000	-	-	-	-	-	-	-	-	1	-	-	-	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-
711276	Bae		Cloning, sequencing and expres ...	Lingulodinium polyedrum	Biochim. Biophys. Acta	1219	449-456	1994	-	-	1	-	-	-	-	-	-	-	1	1	-	6	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-
712332	Morse		Role of a luciferin-binding pr ...	Lingulodinium polyedrum	J. Biol. Chem.	264	11822-11826	1989	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
712539	Nicolas		Characterization of the biolum ...	Lingulodinium polyedrum	J. Cell Biol.	105	723-735	1987	-	-	-	-	-	-	-	-	3	-	-	1	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
712544	Nicolas		The ultrastructural localizati ...	no activity in Cachonina illdefina, Noctiluca miliaris, Pyrocystis fusiformis, Pyrocystis noctiluca	J. Cell Sci.	87	189-196	1987	-	-	-	-	-	-	-	-	6	-	-	-	-	4	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	6	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
712331	Dunlap		The biological clock in Gonyau ...	Lingulodinium polyedrum	J. Biol. Chem.	256	10509-10518	1981	-	-	-	-	-	-	-	1	-	1	1	-	-	2	-	-	1	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	1	-	-	-	-	1	-	-	-	-	1	1	-	-	-	-	-	-	-	-	1	-	-	1	-	-
710987	Lecuyer		Dinoflagellate luciferases: pu ...	Lingulodinium polyedrum, Pyrocystis fusiformis, Pyrocystis lunula	Arch. Biochem. Biophys.	196	371-384	1979	-	-	-	-	-	-	48	-	-	9	3	-	6	3	-	-	3	-	-	-	3	-	3	3	3	-	3	-	3	-	3	-	-	3	-	-	-	-	-	-	-	-	-	48	-	-	-	9	3	-	6	-	-	3	-	-	3	-	3	3	3	-	3	-	3	-	3	3	-	-	-	-	-	-