Biochemical characterization of a trypanosome enzyme with glutathione-dependent peroxidase activity

Wilkinson, S.R.; Meyer, D.J.; Kelly, J.M.; Biochem. J. 352, 755-761 (2000)

 show all sequences of 1.11.1.12

Data extracted from this reference:

Cloned(Commentary)
Commentary Organism
His-tagged protein termed TcGPX1, expression in Escherichia coli Trypanosoma cruzi
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.00058
-
 linoleic acid hydroperoxide recombinant enzyme Trypanosoma cruzi
-
0.00074
-
 L-alpha-phosphatidylcholine hydroperoxide recombinant enzyme Trypanosoma cruzi
-
0.0017
-
 cumene hydroperoxide recombinant enzyme Trypanosoma cruzi
0.0056
-
 tert-butyl hydroperoxide recombinant enzyme Trypanosoma cruzi
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Trypanosoma cruzi
-
 American trypanosome
-
Purification (Commentary)
Commentary Organism
recombinant enzyme from Escherichia coli Trypanosoma cruzi
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
glutathione + cumene hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + H2O2
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + L-alpha-phosphatidylcholine hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + linoleic acid hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + lipid hydroperoxide
-
 394875 Trypanosoma cruzi glutathione disulfide + lipid + H2O
-
-
-
 ?
glutathione + tert-butyl hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
Cloned(Commentary) (protein specific)
Commentary Organism
His-tagged protein termed TcGPX1, expression in Escherichia coli Trypanosoma cruzi
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.00058
-
 linoleic acid hydroperoxide recombinant enzyme Trypanosoma cruzi
-
0.00074
-
 L-alpha-phosphatidylcholine hydroperoxide recombinant enzyme Trypanosoma cruzi
-
0.0017
-
 cumene hydroperoxide recombinant enzyme Trypanosoma cruzi
0.0056
-
 tert-butyl hydroperoxide recombinant enzyme Trypanosoma cruzi
Purification (Commentary) (protein specific)
Commentary Organism
recombinant enzyme from Escherichia coli Trypanosoma cruzi
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
glutathione + cumene hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + H2O2
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + L-alpha-phosphatidylcholine hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + linoleic acid hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?
glutathione + lipid hydroperoxide
-
 394875 Trypanosoma cruzi glutathione disulfide + lipid + H2O
-
-
-
 ?
glutathione + tert-butyl hydroperoxide
-
 394875 Trypanosoma cruzi ?
-
-
-
 ?

See also following references to EC number 1.11.1.12 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
697468HuMolecular characterization of ...Bombus ignitusComp. Biochem. Physiol. B15554-612010--1----2-111-4--1--6--3-1-1-21-1-----11------2-111---1-6--3-1-1-21--1--1--
696173DongEnzymatic properties of a reco ...Momordica charantiaBiochemistry (Moscow)74502-5082009--1----3-111-5--1-----4111--11-1-----11------3-111---1----4111--11--------
696435ConradTransgenic mouse models for th ...Mus musculusBiochim. Biophys. Acta17901575-15852009--------5111-4-----4--21-------1------1-------5111-----4--21--------------
696970BaeModular evolution of glutathio ...animalBMC Evol. Biol.91-132009-1---------1-2--------1--------1----1-1----------1--------1---------------
697470HermeszIdentification of two phosphol ...Cyprinus carpioComp. Biochem. Physiol. C150101-1062009--2------1-1-3-----8--11-------1-----21--------1-1-----8--11--------4--4--
697778KadotaEnhanced metallothionein gene ...Rattus norvegicusEur. J. Pharmacol.626166-1702009--------1--1-2-----1--1--------1------1-------1--1-----1--1---------------
698037Chen-Putative phospholipid hydroper ...Taiwanofungus camphoratusFood Chem.115476-4822009--1-------1--1--1--1--111---1--1-----11---------1----1-1--111---1---------
701033Li-Tissue and induction expressio ...Oryza sativaProg. Biochem. Biophys.3677-8220092------------1-----4--1--------1---2--1----------------4--1---------1--1--
701138DimastrogiovanniCombining crystallography and ...Schistosoma mansoniProteins78259-2702009--112----111-3--1--1--311---1--1-----1112------111---1-1--311---1---------
701272StradaioliPhospholipid hydroperoxide glu ...Bos taurusTheriogenology7291-982009-1-------1-1-3-----48-2-1---1--1----1-1--------1-1-----48-2-1---1---1--1--
686888DabkowskiMitochondria-specific transgen ...Mus musculusFree Radic. Biol. Med.45855-8652008--------2----4-----1--1-----------------------3--------1--1---------------
687186CaiVitellocyte-specific expressio ...Clonorchis sinensisInt. J. Parasitol.381613-16232008--1----------7-----2-----------------4-----------------4------------------
698045GuoSuppression of atherogenesis b ...Homo sapiensFree Radic. Biol. Med.44343-3522008-11------111-3-----1--31----1--1----111--------111-----1--31----1---------
701060KernstockNew strategies for the isolati ...Rattus norvegicusProtein Expr. Purif.62216-2222008----------1--3--1--12-3-------------------------1----1-12-3---------------
701118ZhangCrystal structure of glutathio ...Saccharomyces cerevisiaeProteins Struct. Funct. Genet.731058-10622008--111--------2--1-----1-----1--------1-11------------1----1-----1---------
685185ScheererStructural basis for catalytic ...Homo sapiensBiochemistry469041-90492007--11----1----3-----2---1-------------1-1------1--------2---1--------------
685440ConradPhysiological role of phosphol ...Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofaBiol. Chem.3881019-10252007--------1--2-7-----1--2-----------------------1--2-----1--2---------------
686071WangUrea-induced inactivation and ...Oryza sativaChem. Res. Chin. Univ.23562-5662007--1---------71--1--------------------1------------7--1--------------------
686405PuglisiMice overexpressing the mitoch ...Mus musculusEndocrinology1484302-43092007--1-----5----6-----6-----------------1--------5--------6------------------
686896CejasPhospholipid hydroperoxide glu ...Homo sapiensFree Radic. Res.41681-6872007-1-----------3-----2----------------1------------------2------------------
687471WangPurification and physicochemic ...Oryza sativaJ. Biochem. Mol. Biol.40412-4182007--1----4-----5--1-----4111--11---1---1-------4-------1----4111--11-1------
688464BaekTissue expression and cellular ...Mus musculusJ. Mol. Histol.38237-2442007-------------3-----22-----------------------------------22------------------
688724BaekEffects of endocrine disruptin ...Rattus norvegicusJ. Vet. Sci.8213-2182007-1-----------3-----4----------------1------------------4------------------
688924DingPhospholipid hydroperoxide glu ...Homo sapiensMol. Cancer Ther.61467-14742007-------------3-----9-----------------------------------9------------------
689838ZafiriouBiological role of hepoxilins: ...Rattus norvegicusProstaglandins Leukot. Essent. Fatty Acids77209-2152007-------------2-----1-----------------------------------1------------------
671327DiaconuFailure of phospholipid hydrop ...Homo sapiensAndrologia38152-1572006-------------3-----20-----------------------------------20------------------
672372JanuelPhospholipid-hydroperoxide glu ...Homo sapiensBiochim. Biophys. Acta17611228-12342006------1-3----2--1--24-11-------------------1--3------1-24-11--------------
673807SteinbrennerSelenoprotein P protects endot ...Homo sapiensFree Radic. Res.40936-94320062-----1-1----3-----3--1--------1---2--1----1--1--------3--1---------------
673837DashMolecular characterization of ...Hydra vulgarisGene3811-122006--------2-1--4--------1-----------------------2-1---------1---------------
673845SchneiderEmbryonic expression profile o ...Mus musculusGene Expr. Patterns6489-4942006--------3----3-----18--1-----------------------3--------18--1---------------
673931LiuSuppression of the malignant p ...Homo sapiensHum. Gene Ther.17105-1162006-11-----1----4-----1--1-------------11--------1--------1--1---------------
674370ImaiIdentification of the positive ...Mus musculusJ. Biochem.140573-5902006--------4----3-----13--1-----------------------4--------13--1---------------
674598BorchertThe role of phospholipid hydro ...Mus musculusJ. Biol. Chem.28119655-196642006--------4----4-----2--------------------------4--------2------------------
676554YangA plant mitochondrial phosphol ...Raphanus sativusPlant Mol. Biol.62951-9622006--1-----2-2--5--1--1--3-11--11-1-----11-------2-2----1-1--3-11--11--------
658251NagdasTyrosine phosphorylation gener ...Mesocricetus auratusBiol. Reprod.72164-1712005--------1--1-3-1---1--1-----------------------1--1--1--1--1---------------
658758MaiorinoPrimary structure of the nucle ...Rattus norvegicusFEBS Lett.579667-6702005---------1---3-----3---1-----------------------1-------3---1--------------
671713HattoriInduction of phospholipid hydr ...Homo sapiensBiochem. Biophys. Res. Commun.337464-4732005-------------3-----4-----------------------------------4------------------
671833HattoriUp-regulation of phospholipid ...Rattus norvegicusBiochem. J.389279-2872005-------------3-----3--1--------------------------------3--1---------------
673176PuglisiPHGPx in spermatogenesis: how ...Rattus norvegicusContraception72291-2932005--------4-2--2-----1--------------------------4-2------1------------------
673343LapointeEstrogen selectively up-regula ...Bos taurusEndocrinology1462583-259220051-------1----3-----1--1------------1----------1--------1--1---------------
674008Cossio-BayugarMolecular cloning of a phospho ...Rhipicephalus microplusInsect Biochem. Mol. Biol.351378-13872005---------1---5---------------------------------1--------------------------
674502MaiorinoFunctional interaction of phos ...Rattus norvegicusJ. Biol. Chem.28038395-384022005--------1----2-----110-10--------1------1-------1--------110-10---------------
675048KriskaA thin layer chromatographic m ...Homo sapiensJ. Chromatogr. B82758-642005--1----------2-----1--2--------------1-----------------1--2---------------
675965ConradThe nuclear form of phospholip ...Mus musculusMol. Cell. Biol.257637-76442005--------2----3-----4--------------------------2--------4------------------
658926TramerNative specific activity of gl ...Homo sapiensInt. J. Androl.2788-932004-------------2-----2-----------------------------------2------------------
659398UtomoIdentification of a novel puta ...Homo sapiensJ. Biol. Chem.27943522-435292004--------1--1-3-----10--1-----------------------1--1-----10--1---------------
659647LiPossible function of two insec ...Drosophila melanogaster, Venturia canescensJ. Insect Physiol.491-92003--2--------2-5--2-----5--------------2-----------2---2----5---------------
394877TramerEnzymatic and immunochemical e ...Rattus norvegicusInt. J. Androl.2572-832002--1-----211--3--1--51--1-------------1--------211----1-51--1--------------
658247ForestaMale fertility is linked to th ...Homo sapiensBiol. Reprod.67967-9712002-1-------1-1-3-----2--1-------------1----------1-1-----2--1---------------
658606HerbetteTwo GPX-like proteins from Lyc ...Solanum lycopersicumEur. J. Biochem.2692414-24202002--1----8-----4--------5--------------1-------8------------5---------------
394874AverySaccharomyces cerevisiae expre ...Saccharomyces cerevisiaeJ. Biol. Chem.27633730-337352001--1-11-----1-4--1-----2--------------1--11-------1---1----2---------------
394875WilkinsonBiochemical characterization o ...Trypanosoma cruziBiochem. J.352755-7612000--1----4-----1--1-----6--------------1-------4-------1----6---------------
394879SchnurrBacterial and nonbacterial exp ...Homo sapiensProtein Expr. Purif.19403-4102000--1-1----1-2-4--1--11-3-1--11--------1--1------1-2---1-11-3-1--11---------
394883NomuraMitochondrial phospholipid hyd ...Rattus norvegicusBiochem. J.351183-1932000--1-1---1--2-3-----1--4--------------1--1-----1--2-----1--4---------------
394872Avsian-KretchmerRegulation of stress-induced p ...Citrus sinensisPlanta209469-4771999-----------1-3-----1--2---1----------------------1-----1--2---1-----------
394873SaitoSelenoprotein P in human plasm ...Homo sapiensJ. Biol. Chem.2742866-287119992-------1111-3--11-21-511---1------2----------1111---1-21-511---1---------
394882MaiorinoA selenium-containing phosphol ...Schistosoma mansoniEur. J. Biochem.238838-8441996------1-111--2--1---2-41-------------------1--111----1--2-41--------------
394878ChambersPurification of a cytosolic en ...Homo sapiensInt. J. Biochem.261279-12861994------1-2113-4--1--2528-1--11--------------1--2113---1-2528-1--11---------
394880RoveriPurification and characterizat ...Rattus norvegicusBiochim. Biophys. Acta1208211-2211994-----1--3112-3--1--33-821---1------------1----3112---1-33-821---1---------
394881SattlerReduction of HDL- and LDL-asso ...Sus scrofaArch. Biochem. Biophys.309214-2211994-------------1-----11-3-1---1--------------------------11-3-1---1---------
394876LamPurification of phospholipid h ...Bos taurusCurr. Eye Res.129-151993------12111--3--1--21-41--------1----------112111----1-21-41--------------
394866PanfiliDistribution of glutathione pe ...Rattus norvegicusFEBS Lett.29035-371991--------4----3--1--2--------------------------4------1-2------------------
394867MaiorinoPhospholipid hydroperoxide glu ...Bos taurus, Canis lupus familiaris, Rattus norvegicus, Sus scrofaMethods Enzymol.186448-4571990--------6448-4--11-20213342---4-----------------6448---1-20213342---4---------
394870ThomasEnzymatic reduction of phospho ...Sus scrofaBiochim. Biophys. Acta1045252-2601990---------1-2-2-----1--4------------------------1-2-----1--4---------------
394871ZhangPhospholipid hydroperoxide glu ...Rattus norvegicusBiochim. Biophys. Acta1006140-1431989--------1----3-----61-5-----------------------1--------61-5---------------
394865MaiorinoDifferent effects of Triton X- ...Sus scrofaArch. Biochem. Biophys.251600-60519861-----2----1-2---1-1--6------------1-------2-----1-----1--6---------------
394864UrsiniThe selenoenzyme phospholipid ...Sus scrofaBiochim. Biophys. Acta83962-7019851-----1-111--3--11-11-101----1------1-------1--111----1-11-101----1---------
394868UrsiniPurification from pig liver of ...Sus scrofaBiochim. Biophys. Acta710197-2111982----------12-4--1--24-61--1---------------------12---1-24-61--1-----------
394869Maiorino-A pig heart peroxidation inhib ...Sus scrofaBiochem. Int.5575-5811982-------------1--1--1--1------------------------------1-1--1---------------