Purification and properties of a new enzyme from Evernia prunastri, which reduces L-usnic acid

Estevez, M.P.; Legaz, E.; Olmeda, L.; Perez, F.J.; Vicente, C.; Z. Naturforsch. C 36, 35-39 (1981)

Data extracted from this reference:

General Stability

General Stability	Organism
thermolabile	Evernia prunastri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
450000	-	gel filtration	Evernia prunastri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
L-usnic acid + NADH	Evernia prunastri	-	NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Evernia prunastri	-	-	-

Purification (Commentary)

Commentary	Organism
-	Evernia prunastri

Source Tissue

Source Tissue	Commentary	Organism	Textmining
thallus	-	Evernia prunastri	-

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
L-usnic acid + NADH	-	389485	Evernia prunastri	NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone	-	-	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
30	-	-	Evernia prunastri

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Commentary	Organism
22	37	-	Evernia prunastri
22	-	about 30% of maximal activity	Evernia prunastri
37	-	about 35% of maximal activity	Evernia prunastri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
8	-	-	Evernia prunastri

pH Range

pH Minimum	pH Maximum	Commentary	Organism
6.8	9.2	-	Evernia prunastri
6.8	-	about 20% of maximal activity	Evernia prunastri
9.2	-	about 50% of maximal activity	Evernia prunastri

Cofactor

Cofactor	Commentary	Organism	Structure
NADH	strict requirement for NADH, not replaceable by NADPH	Evernia prunastri

Cofactor (protein specific)

Cofactor	Commentary	Organism	Structure
NADH	strict requirement for NADH, not replaceable by NADPH	Evernia prunastri

General Stability (protein specific)

General Stability	Organism
thermolabile	Evernia prunastri

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
450000	-	gel filtration	Evernia prunastri

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
L-usnic acid + NADH	Evernia prunastri	-	NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone	-	-	?

Purification (Commentary) (protein specific)

Commentary	Organism
-	Evernia prunastri

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
thallus	-	Evernia prunastri	-

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
L-usnic acid + NADH	-	389485	Evernia prunastri	NAD+ + 2-acetyl-6-(3-acetyl-2,4,6-trihydroxy-5-methyl-phenyl)-3-hydroxy-6-methyl-cyclohexa-2,4-dienone	-	-	-	?

Temperature Optimum [°C] (protein specific)

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Commentary	Organism
30	-	-	Evernia prunastri

Temperature Range [°C] (protein specific)

Temperature Minimum [°C]	Temperature Maximum [°C]	Commentary	Organism
22	37	-	Evernia prunastri
22	-	about 30% of maximal activity	Evernia prunastri
37	-	about 35% of maximal activity	Evernia prunastri

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
8	-	-	Evernia prunastri

pH Range (protein specific)

pH Minimum	pH Maximum	Commentary	Organism
6.8	9.2	-	Evernia prunastri
6.8	-	about 20% of maximal activity	Evernia prunastri
9.2	-	about 50% of maximal activity	Evernia prunastri

See also following references to EC number 1.1.1.199 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
389487	Avalos	-	Action of filipin on phytochro ...	Evernia prunastri	J. Plant Physiol.	135	478-482	1989	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
389486	Avalos		Equivalence between Pfr and cy ...	Evernia prunastri	Plant Physiol.	84	803-807	1987	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	1	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	1	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
389485	Estevez	-	Purification and properties of ...	Evernia prunastri	Z. Naturforsch. C	36	35-39	1981	-	-	-	-	-	1	-	-	-	-	1	1	-	1	-	-	1	-	-	1	-	-	1	-	1	3	-	-	1	3	-	1	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	1	1	-	-	-	1	-	1	-	-	1	-	1	3	-	-	1	3	-	-	-	-	-	-	-	-