L-Fucose dehydrogenase from sheep liver

Mobley, P.W.; Metzger, R.P.; Wick, A.N.; Methods Enzymol. 41B, 173-177 (1975)

Data extracted from this reference:

General Stability

General Stability	Organism
stable to refrigeration at 5°C for a week	Ovis aries aries

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.01	-	NAD+	at pH 10.4	Ovis aries aries
0.04	-	NAD+	at pH 8.6	Ovis aries aries
0.07	-	6-deoxy-L-galactose	at pH 8.6	Ovis aries aries
0.4	-	D-arabinose	at pH 8.6	Ovis aries aries
1.5	-	6-deoxy-L-galactose	at pH 10.4	Ovis aries aries
7.3	-	D-arabinose	at pH 10.4	Ovis aries aries

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
96000	-	electro focusing	Ovis aries aries

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
2-deoxy-D-ribose + NAD+	Ovis aries aries	10% of the activity compared to L-fucose or D-arabinose	? + NADH	-	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	Ovis aries aries	ordered bi-bi-reaction mechanism	6-deoxy-L-galactono-1,5-lactone + NADH	-	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	Ovis aries aries	i.e. L-fucose	6-deoxy-L-galactono-1,5-lactone + NADH	-	Ovis aries aries	?
D-arabinose + NAD+	Ovis aries aries	-	D-arabino-1,5-lactone + NADH	-	Ovis aries aries	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Ovis aries aries	-	sheep	-

Purification (Commentary)

Commentary	Organism
to homogeneity, chromatography techniques	Ovis aries aries

Source Tissue

Source Tissue	Commentary	Organism	Textmining
liver	-	Ovis aries aries	-

Storage Stability

Storage Stability	Organism
-20°C, minimum concentration: 1 mg/ml protein, 1 month	Ovis aries aries

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
2-deoxy-D-ribose + NAD+	10% of the activity compared to L-fucose or D-arabinose	285804	Ovis aries aries	? + NADH	-	285804	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	ordered bi-bi-reaction mechanism	285804	Ovis aries aries	6-deoxy-L-galactono-1,5-lactone + NADH	-	285804	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	i.e. L-fucose	285804	Ovis aries aries	6-deoxy-L-galactono-1,5-lactone + NADH	-	285804	Ovis aries aries	?
D-arabinose + NAD+	-	285804	Ovis aries aries	D-arabino-1,5-lactone + NADH	-	285804	Ovis aries aries	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Commentary	Organism
60	-	stable up to	Ovis aries aries

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
10.4	-	ionic strength: 0.05	Ovis aries aries

Cofactor

Cofactor	Commentary	Organism	Structure
NAD+	-	Ovis aries aries

Cofactor (protein specific)

Cofactor	Commentary	Organism	Structure
NAD+	-	Ovis aries aries

General Stability (protein specific)

General Stability	Organism
stable to refrigeration at 5°C for a week	Ovis aries aries

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.01	-	NAD+	at pH 10.4	Ovis aries aries
0.04	-	NAD+	at pH 8.6	Ovis aries aries
0.07	-	6-deoxy-L-galactose	at pH 8.6	Ovis aries aries
0.4	-	D-arabinose	at pH 8.6	Ovis aries aries
1.5	-	6-deoxy-L-galactose	at pH 10.4	Ovis aries aries
7.3	-	D-arabinose	at pH 10.4	Ovis aries aries

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
96000	-	electro focusing	Ovis aries aries

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
2-deoxy-D-ribose + NAD+	Ovis aries aries	10% of the activity compared to L-fucose or D-arabinose	? + NADH	-	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	Ovis aries aries	ordered bi-bi-reaction mechanism	6-deoxy-L-galactono-1,5-lactone + NADH	-	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	Ovis aries aries	i.e. L-fucose	6-deoxy-L-galactono-1,5-lactone + NADH	-	Ovis aries aries	?
D-arabinose + NAD+	Ovis aries aries	-	D-arabino-1,5-lactone + NADH	-	Ovis aries aries	?

Purification (Commentary) (protein specific)

Commentary	Organism
to homogeneity, chromatography techniques	Ovis aries aries

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
liver	-	Ovis aries aries	-

Storage Stability (protein specific)

Storage Stability	Organism
-20°C, minimum concentration: 1 mg/ml protein, 1 month	Ovis aries aries

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
2-deoxy-D-ribose + NAD+	10% of the activity compared to L-fucose or D-arabinose	285804	Ovis aries aries	? + NADH	-	285804	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	ordered bi-bi-reaction mechanism	285804	Ovis aries aries	6-deoxy-L-galactono-1,5-lactone + NADH	-	285804	Ovis aries aries	?
6-deoxy-L-galactose + NAD+	i.e. L-fucose	285804	Ovis aries aries	6-deoxy-L-galactono-1,5-lactone + NADH	-	285804	Ovis aries aries	?
D-arabinose + NAD+	-	285804	Ovis aries aries	D-arabino-1,5-lactone + NADH	-	285804	Ovis aries aries	?

Temperature Stability [°C] (protein specific)

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Commentary	Organism
60	-	stable up to	Ovis aries aries

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
10.4	-	ionic strength: 0.05	Ovis aries aries

See also following references to EC number 1.1.1.122 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
655086	Bakke	-	Improvement in thermal stabili ...	Pseudomonas sp.	Biotechnol. Lett.	24	1075-1078	2002	-	-	-	-	-	1	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	1	-	-	-	2	-	-	-	-	1	-	-	-	-	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-
285798	Itoh	-	Purification and characterizat ...	Agrobacterium tumefaciens	J. Ferment. Bioeng.	77	100-102	1994	-	-	-	-	-	-	4	5	-	-	1	3	-	2	-	-	1	-	-	1	2	-	4	-	1	-	1	-	1	-	1	1	-	-	-	-	-	-	1	-	-	-	-	4	-	5	-	-	1	3	-	-	-	1	-	1	2	-	4	-	1	-	1	-	1	-	1	-	-	-	-	-	-	-
285799	Yamamoto-Otake		Cloning and sequencing of the ...	Pseudomonas sp.	Biosci. Biotechnol. Biochem.	58	2281-2282	1994	-	-	1	-	-	-	-	-	-	-	-	1	-	4	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
285800	Tsuji		Purification and some properti ...	Agrobacterium tumefaciens	Biochim. Biophys. Acta	1117	167-173	1992	-	1	-	-	-	1	4	4	-	-	1	3	-	4	-	-	1	-	-	1	1	1	4	1	-	-	1	-	-	1	1	2	-	-	-	-	1	-	2	-	-	1	-	4	-	4	-	-	1	3	-	-	-	1	-	1	1	1	4	1	-	-	1	-	-	1	1	-	-	-	-	-	-	-
285801	Guimaraes	-	Purification and general prope ...	Aureobasidium pullulans	Arq. Biol. Tecnol. (Curitiba)	32	575-587	1989	-	-	-	-	-	-	2	4	-	-	1	3	-	1	-	-	1	-	-	1	-	1	3	1	-	-	1	-	1	-	-	1	-	-	-	-	-	-	1	-	-	-	-	2	-	4	-	-	1	3	-	-	-	1	-	1	-	1	3	1	-	-	1	-	1	-	-	-	-	-	-	-	-	-
285802	Sasajima		Oxidation of L-glucose by a ps ...	Burkholderia caryophylli	Biochim. Biophys. Acta	571	120-126	1979	-	-	-	-	-	1	4	4	-	-	1	7	-	1	-	-	1	-	-	1	1	1	7	-	-	-	-	-	1	-	-	2	-	-	-	-	-	-	2	-	-	1	-	4	-	4	-	-	1	7	-	-	-	1	-	1	1	1	7	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-
285805	Endo		Isolation and characterization ...	Oryctolagus cuniculus	J. Biochem.	86	1559-1565	1979	-	-	-	-	-	-	4	3	-	-	1	4	-	3	-	-	1	-	-	2	1	1	4	-	1	-	1	-	1	-	1	1	-	-	-	-	-	-	1	-	-	-	-	4	-	3	-	-	1	4	-	-	-	1	-	2	1	1	4	-	1	-	1	-	1	-	1	-	-	-	-	-	-	-
285803	Mobley		The physical properties of NAD ...	Ovis aries aries	Arch. Biochem. Biophys.	186	184-188	1978	5	-	-	-	-	1	3	1	-	-	1	2	-	2	-	-	1	-	-	2	-	1	2	1	-	-	2	-	-	-	-	1	-	-	-	5	-	-	1	-	-	1	-	3	-	1	-	-	1	2	-	-	-	1	-	2	-	1	2	1	-	-	2	-	-	-	-	-	-	-	-	-	-	-
285804	Mobley		L-Fucose dehydrogenase from sh ...	Ovis aries aries	Methods Enzymol.	41B	173-177	1975	-	-	-	-	-	1	-	6	-	-	1	4	-	2	-	-	1	-	-	2	-	1	4	-	-	-	1	-	1	-	-	1	-	-	-	-	-	-	1	-	-	1	-	-	-	6	-	-	1	4	-	-	-	1	-	2	-	1	4	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-
285806	Schacter		Isolation of diphosphopyridine ...	Sus scrofa	J. Biol. Chem.	244	4785-4792	1969	1	-	-	-	-	-	4	7	-	-	-	6	-	2	-	-	1	-	-	2	1	-	6	-	-	-	-	-	1	-	-	1	-	-	-	1	-	-	1	-	-	-	-	4	-	7	-	-	-	6	-	-	-	1	-	2	1	-	6	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-