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Literature summary extracted from

  • Thangavelu, K.; Chong, Q.Y.; Low, B.C.; Sivaraman, J.
    Structural basis for the active site inhibition mechanism of human kidney-type glutaminase (KGA) (2014), Sci. Rep., 4, 3827.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.2 expressed in Escherichia coli BL21 (DE3)-RIL-Codon plus cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.2 catalytic domain of isoform KGA in complex with 6-diazo-5-oxo-L-norleucine, hanging drop vapor diffusion method, using 0.1 M Bis-Tris propane (pH 7.2) and 1.8 M LiSO4 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.2 K289A the mutant shows about 10% activity compared to the wild type enzyme Homo sapiens
3.5.1.2 S286A the mutant shows about 10% activity compared to the wild type enzyme Homo sapiens
3.5.1.2 Y249A the mutant shows about 10% activity compared to the wild type enzyme Homo sapiens
3.5.1.2 Y466A the mutant shows about 18% activity compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 6-diazo-5-oxo-L-norleucine active site inhibitor, about 53% inhibition at about 1 mM Homo sapiens
3.5.1.2 azaserine about 19% inhibition at about 1 mM Homo sapiens
3.5.1.2 glufosinate ammonium about 23% inhibition at about 1 mM Homo sapiens
3.5.1.2 L-methionine sulfoximine about 21% inhibition at about 1 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Homo sapiens
-
L-glutamate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Homo sapiens O94925
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 Ni-NTA column chromatpgraphy and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.2 kidney
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 6-diazo-5-oxo-L-norleucine + H2O the nucleophilic attack of Ser286 sidechain on 6-diazo-5-oxo-L-norleucine releases the diazo group (N2) from the inhibitor and results in the formation of an enzyme-inhibitor complex Homo sapiens ?
-
?
3.5.1.2 L-glutamine + H2O
-
Homo sapiens L-glutamate + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 GLS1
-
Homo sapiens
3.5.1.2 KGA isoform Homo sapiens
3.5.1.2 kidney-type glutaminase
-
Homo sapiens