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Literature summary extracted from

  • Olkku, A.; Mahonen, A.
    Wnt and steroid pathways control glutamate signalling by regulating glutamine synthetase activity in osteoblastic cells (2008), Bone, 43, 483-493.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.2 MK801 N-methyl-D-aspartate-type glutamate receptor antagonist, activates the enzyme and inhibits osteogenic differentiation of the rat bone marrow stem cells Homo sapiens
6.3.1.2 additional information glucocorticoids induce the enzyme transcription and translation in osteoblasts Homo sapiens
6.3.1.2 additional information glucocorticoids induce the enzyme transcription and translation in osteoblasts, cortisol and two synthetic compounds dexamethasone and prednisolone highly stimulate enzyme expression. No effects by aldosterone, estradiol, progesterone, dihydrotestosterone, or triiodothyronine. Treatment of MG-63 cells with MG-132, an inhibitor of proteasomal degradation, at 0.02 mM blocks the dexamethasone-induction of glutamine synthetase activity almost completely Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expression analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 methionine sulfoximine an irreversible, competitive inhibitor of glutamine synthetase activity Homo sapiens
6.3.1.2 additional information the canonical Wnt signalling pathway is a negative regulator of glutamine synthetase activity; the canonical Wnt signalling pathway is a negative regulator of glutamine synthetase activity. No effects by aldosterone, estradiol, progesterone, dihydrotestosterone, or triiodothyronine Homo sapiens
6.3.1.2 vitamin D 22% inhibition at 0.00001 mM, reduces dexamethasone induced increase in enzyme expression Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+
-
 Homo sapiens
6.3.1.2 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Homo sapiens
-
 ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 additional information Homo sapiens Wnt and steroid pathways control glutamate signalling by regulating glutamine synthetase activity in osteoblastic cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 bone marrow stem cell
-
 Homo sapiens
-
6.3.1.2 MG-63 cell osteoblast-like cells Homo sapiens
-
6.3.1.2 additional information the enzyme is ubiquitously expressed in human tissues Homo sapiens
-
6.3.1.2 osteoblast
-
 Homo sapiens
-
6.3.1.2 osteoblast primary derived from bone marrow Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 0.018
-
 glutamine synthetase activity in MG-63 cells Homo sapiens
6.3.1.2 0.022
-
 glutamine synthetase activity in MG-63 cells in presence of MG-132 Homo sapiens
6.3.1.2 0.023
-
 glutamine synthetase activity in MG-63 cells in presence of dexamethasone and MG-132 Homo sapiens
6.3.1.2 0.042
-
 glutamine synthetase activity in MG-63 cells in presence of vitamin D Homo sapiens
6.3.1.2 0.067
-
 glutamine synthetase activity in MG-63 cells in presence of dexamethasone Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Homo sapiens ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 additional information Wnt and steroid pathways control glutamate signalling by regulating glutamine synthetase activity in osteoblastic cells, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 Glutamine synthetase
-
 Homo sapiens
6.3.1.2 L-glutamate:ammonia ligase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.8
-
 assay at Homo sapiens