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Literature summary extracted from

  • Lent, B.A.; Kim, K.H.
    Purification and properties of a kinase which phosphorylates and inactivates acetyl-CoA carboxylase (1982), J. Biol. Chem., 257, 1897-1901.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.27 CoA most effective at 0.1 mM Rattus norvegicus
2.7.11.27 additional information cAMP independent Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.27 0.00009
-
 acetyl-CoA carboxylase
-
 Rattus norvegicus
2.7.11.27 0.0008
-
 protamine
-
 Rattus norvegicus
2.7.11.27 0.005
-
 histone
-
 Rattus norvegicus
2.7.11.27 0.02
-
 ATP
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.27 170000
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.27 ATP + [acetyl-CoA carboxylase] Rattus norvegicus phosphorylates and inactivates acetyl-CoA carboxylase, (EC 6.4.1.2), and is therefore involved in regulation of long chain fatty acid synthesis ADP + [acetyl-CoA carboxylase] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.27 Rattus norvegicus
-
 Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.27
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.27 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.7.11.27 -20°C, 10 mM phosphate buffer, pH 7, 2 mM EDTA, 10 mM 2-mercaptoethanol, 20% glycerol, 4 weeks, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.27 ATP + acetyl-CoA carboxylase
-
 Rattus norvegicus ADP + phosphorylated acetyl-CoA carboxylase
-
 ?
2.7.11.27 ATP + histone
-
 Rattus norvegicus ADP + phosphohistone
-
 ?
2.7.11.27 ATP + protamine
-
 Rattus norvegicus ADP + protamine phosphate
-
 ?
2.7.11.27 ATP + [acetyl-CoA carboxylase] inactivation of carboxylase Rattus norvegicus ADP + [acetyl-CoA carboxylase] phosphate
-
 ?
2.7.11.27 ATP + [acetyl-CoA carboxylase] phosphorylates and inactivates acetyl-CoA carboxylase, (EC 6.4.1.2), and is therefore involved in regulation of long chain fatty acid synthesis Rattus norvegicus ADP + [acetyl-CoA carboxylase] phosphate
-
 ?
2.7.11.27 additional information no activity with phosphorylase b and 3-hydroxy-3-methylglutaryl-CoA reductase Rattus norvegicus ?
-
 ?