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Literature summary extracted from

  • Cooper, A.J.L.; Meister, A.
    Isolation and properties of a new glutamine transaminase form rat kidney (1974), J. Biol. Chem., 249, 2554-2561.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.64 0.1
-
 2-Oxoglutaramate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
2.6.1.64 0.58
-
 L-phenylalanine pH 8.5, 37°C Rattus norvegicus
2.6.1.64 0.92
-
 alpha-keto-gamma-methiolbutyrate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
2.6.1.64 1.4
-
 L-glutamine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
2.6.1.64 4.2
-
 L-methionine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
2.6.1.64 4.4
-
 L-ethionine pH 8.5, 37°C, phenylpyruvate Rattus norvegicus
2.6.1.64 4.8
-
 glyoxylate pH 8.5, 37°C Rattus norvegicus
2.6.1.64 7.8
-
 alpha-keto-n-valerate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
2.6.1.64 8.7
-
 alpha-keto-n-caproate pH 8.5, 37°C, L-phenylalanine as amino group donor Rattus norvegicus
2.6.1.64 18
-
 alpha-Ketobutyrate pH 8.5, 37°C Rattus norvegicus
2.6.1.64 27
-
 pyruvate pH 8.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.15 cytosol
-
 Rattus norvegicus 5829
-
2.6.1.64 cytoplasm
-
 Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.64 55000
-
 2 * 55000, liver enzyme Rattus norvegicus
2.6.1.64 110000
-
 kidney enzyme, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.15 L-glutamine + alpha-keto acid Rattus norvegicus
-
 alpha-ketoglutarate + amino acid
-
 ?
2.6.1.15 L-glutamine + alpha-keto acid Rattus norvegicus Sprague-Dawley
-
 alpha-ketoglutarate + amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.15 Rattus norvegicus
-
 male Sprague-Dawley
-
2.6.1.64 Rattus norvegicus
-
 Sprague-Dawley
-
2.6.1.64 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.15 DE-52 chromatography, hydroxyapatite chromatography Rattus norvegicus
2.6.1.64
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.15 liver
-
 Rattus norvegicus
-
2.6.1.64 brain
-
 Rattus norvegicus
-
2.6.1.64 cardiac muscle
-
 Rattus norvegicus
-
2.6.1.64 kidney
-
 Rattus norvegicus
-
2.6.1.64 liver
-
 Rattus norvegicus
-
2.6.1.64 additional information no glutamine transaminase activity in skeletal muscle, testis, spleen, pancreas, lung and intestine Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.15 0.467
-
-
 Rattus norvegicus
2.6.1.64 3.68
-
 cytoplasmic isozyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.15 2-oxoglutaramate + L-methionine
-
 Rattus norvegicus L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 gamma-glutamylhydrazones of 2-oxo acid
-
 Rattus norvegicus L-amino acid + 2-hydroxy-tetrahydro-6-pyridazinone-3-carboxylic acid
-
 ?
2.6.1.15 glyoxylate + 4-glutamylmethylamide
-
 Rattus norvegicus glycine + ?
-
 ?
2.6.1.15 glyoxylate + glutamic acid 4-methyl ester
-
 Rattus norvegicus glycine + 2-oxoglutarate 4-methyl ester
-
 ?
2.6.1.15 glyoxylate + L-cysteine
-
 Rattus norvegicus glycine + 3-mercapto-2-oxopropanoate
-
 ?
2.6.1.15 glyoxylate + L-ethionine
-
 Rattus norvegicus glycine + 4-ethylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-ethionine
-
 Rattus norvegicus Sprague-Dawley glycine + 4-ethylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-glutamine
-
 Rattus norvegicus glycine + 2-oxoglutaramate
-
 ?
2.6.1.15 glyoxylate + L-glutamine
-
 Rattus norvegicus Sprague-Dawley glycine + 2-oxoglutaramate
-
 ?
2.6.1.15 glyoxylate + L-homoserine
-
 Rattus norvegicus glycine + 4-hydroxy-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-methionine
-
 Rattus norvegicus glycine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-methionine
-
 Rattus norvegicus Sprague-Dawley glycine + 4-methylsulfanyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-methionine (SR)-sulfoxide
-
 Rattus norvegicus glycine + ?
-
 ?
2.6.1.15 glyoxylate + L-methionine (SR)-sulfoxide
-
 Rattus norvegicus Sprague-Dawley glycine + ?
-
 ?
2.6.1.15 glyoxylate + L-methionine sulfone
-
 Rattus norvegicus glycine + 4-methylsulfonyl-2-oxobutanoate
-
 ?
2.6.1.15 glyoxylate + L-phenylalanine
-
 Rattus norvegicus glycine + phenylpyruvate
-
 ?
2.6.1.15 glyoxylate + S-methyl-L-cysteine
-
 Rattus norvegicus glycine + 3-methylsulfanyl-2-oxopropanoate
-
 ?
2.6.1.15 L-glutamine + alpha-keto acid
-
 Rattus norvegicus alpha-ketoglutarate + amino acid
-
 ?
2.6.1.15 L-glutamine + alpha-keto acid
-
 Rattus norvegicus Sprague-Dawley alpha-ketoglutarate + amino acid
-
 ?
2.6.1.15 pyruvate + L-glutamine
-
 Rattus norvegicus L-alanine + 2-oxoglutaramate
-
 ?
2.6.1.15 pyruvate + L-phenylalanine
-
 Rattus norvegicus L-alanine + phenylpyruvate
-
 ?
2.6.1.64 4-cyano-L-alpha-aminobutyrate + glyoxylate
-
 Rattus norvegicus 4-cyano-2-oxobutanoate + glycine
-
 r
2.6.1.64 4-cyano-L-alpha-aminobutyrate + phenylpyruvate
-
 Rattus norvegicus 4-cyano-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 albizziin + glyoxylate
-
 Rattus norvegicus 3-carbamido-2-oxopropanoate + glycine
-
 r
2.6.1.64 DL-homoserine + glyoxylate
-
 Rattus norvegicus 4-hydroxy-2-oxobutanoate + glycine
-
 r
2.6.1.64 DL-homoserine + phenylpyruvate
-
 Rattus norvegicus 4-hydroxy-2-oxobutanoate + phenylalanine
-
 r
2.6.1.64 L-cysteine + glyoxylate
-
 Rattus norvegicus 3-mercapto-2-oxopropanoate + glycine
-
 r
2.6.1.64 L-cysteine + phenylpyruvate
-
 Rattus norvegicus 3-mercapto-2-oxopropanoate + L-phenylalanine
-
 r
2.6.1.64 L-ethionine + glyoxylate
-
 Rattus norvegicus 4-ethylsulfanyl-2-oxobutanoate + glycine
-
 r
2.6.1.64 L-ethionine + phenylpyruvate
-
 Rattus norvegicus 4-ethylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-gamma-glutamylhydrazone of glyoxylate + glyoxylate
-
 Rattus norvegicus ?
-
 r
2.6.1.64 L-gamma-glutamylhydrazone of glyoxylate + phenylpyruvate
-
 Rattus norvegicus ?
-
 r
2.6.1.64 L-gamma-glutamylhydrazone of phenylpyruvate + glyoxylate
-
 Rattus norvegicus ?
-
 r
2.6.1.64 L-gamma-glutamylhydrazone of phenylpyruvate + phenylpyruvate
-
 Rattus norvegicus ?
-
 r
2.6.1.64 L-glutamic acid-gamma-methyl ester + glyoxylate liver enzyme L Rattus norvegicus 2-oxo-pentandioate-5-methyl ester + glycine
-
 r
2.6.1.64 L-glutamic acid-gamma-methyl ester + phenylpyruvate liver enzyme L Rattus norvegicus 2-oxo-pentandioate-5-methyl ester + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + 2-oxobutyrate
-
 Rattus norvegicus 2-oxoglutaramate + 2-aminobutanoate
-
 r
2.6.1.64 L-glutamine + 2-oxoglutaramate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamine
-
 r
2.6.1.64 L-glutamine + 2-oxoglutarate
-
 Rattus norvegicus 2-oxoglutaramate + L-glutamate
-
 r
2.6.1.64 L-glutamine + 2-oxovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-norvaline
-
 r
2.6.1.64 L-glutamine + 3-hydroxypyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-serine
-
 r
2.6.1.64 L-glutamine + alpha-keto-delta-carbamidovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-arginine
-
 r
2.6.1.64 L-glutamine + alpha-keto-n-caproate
-
 Rattus norvegicus 2-oxoglutaramate + L-norleucine
-
 r
2.6.1.64 L-glutamine + alpha-ketoisocaproate
-
 Rattus norvegicus 2-oxoglutaramate + L-leucine
-
 r
2.6.1.64 L-glutamine + alpha-ketoisovalerate
-
 Rattus norvegicus 2-oxoglutaramate + L-valine
-
 r
2.6.1.64 L-glutamine + glyoxylate isozyme L highly active, isozyme K relatively little activity toward glyoxylate Rattus norvegicus 2-oxoglutaramate + glycine
-
 r
2.6.1.64 L-glutamine + oxaloacetate
-
 Rattus norvegicus 2-oxoglutaramate + L-aspartate
-
 r
2.6.1.64 L-glutamine + p-hydroxyphenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-tyrosine
-
 r
2.6.1.64 L-glutamine + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutaramate + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + pyruvate isozyme L highly active, isozyme K relatively little activity toward pyruvate Rattus norvegicus 2-oxoglutaramate + L-alanine
-
 r
2.6.1.64 L-glutamylmethylamide + glyoxylate liver enzyme L Rattus norvegicus 2-oxoglutarate-5-methylamide + glycine
-
 r
2.6.1.64 L-glutamylmethylamide + phenylpyruvate
-
 Rattus norvegicus 2-oxoglutarate-5-methylamide + L-phenylalanine
-
 r
2.6.1.64 L-histidine + pyruvate poor substrates Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 r
2.6.1.64 L-methionine + 2-oxoglutaramate isozymes L and K highly active Rattus norvegicus L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
 r
2.6.1.64 L-methionine + glyoxylate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + glycine
-
 r
2.6.1.64 L-methionine + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-methionine sulfone + glyoxylate
-
 Rattus norvegicus 4-methylsulfonyl-2-oxobutanoate + glycine
-
 r
2.6.1.64 L-methionine sulfone + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfonyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-methionine-SR-sulfoxide + glyoxylate
-
 Rattus norvegicus 4-methylsulfinyl-2-oxobutanoate + glycine
-
 r
2.6.1.64 L-methionine-SR-sulfoxide + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfinyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-methionine-SR-sulfoximine + glyoxylate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + glycine
-
 r
2.6.1.64 L-methionine-SR-sulfoximine + phenylpyruvate
-
 Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate-SR-sulfoximine + L-phenylalanine
-
 r
2.6.1.64 L-phenylalanine + 2-oxoglutaramate
-
 Rattus norvegicus phenylpyruvate + L-glutamine
-
 r
2.6.1.64 L-phenylalanine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Rattus norvegicus phenylpyruvate + L-methionine
-
 r
2.6.1.64 L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate isozyme K very active toward phenylalanine Rattus norvegicus phenylpyruvate + L-methionine
-
 r
2.6.1.64 L-phenylalanine + alpha-keto-n-caproate
-
 Rattus norvegicus phenylpyruvate + L-norleucine
-
 r
2.6.1.64 L-phenylalanine + alpha-keto-n-valerate
-
 Rattus norvegicus phenylpyruvate + L-norvaline
-
 r
2.6.1.64 L-phenylalanine + alpha-ketobutyrate
-
 Rattus norvegicus phenylpyruvate + 2-aminobutyrate
-
 r
2.6.1.64 L-phenylalanine + glyoxylate
-
 Rattus norvegicus phenylpyruvate + glycine
-
 r
2.6.1.64 L-phenylalanine + pyruvate
-
 Rattus norvegicus phenylpyruvate + L-alanine
-
 r
2.6.1.64 L-tyrosine + 2-oxoglutaramate
-
 Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine
-
 r
2.6.1.64 additional information 4 separate isozymes, cytoplasmic and mitochondrial, in kidney and liver, trimethylpyruvate and D and L-alpha-keto-beta-methylvalerate are no amino acceptors, L-gamma-glutamyldimethylamide is no substrate Rattus norvegicus ?
-
 ?
2.6.1.64 O-carbamoyl-L-serine + glyoxylate
-
 Rattus norvegicus O-carbamoyl-(3-hydroxy-2-oxo-propanoate) + glycine
-
 r
2.6.1.64 S-carbamoyl-L-cysteine + glyoxylate
-
 Rattus norvegicus S-carbamoyl-3-mercapto-2-oxo-propanoate + glycine
-
 r
2.6.1.64 S-methyl-L-cysteine + glyoxylate
-
 Rattus norvegicus 3-methylsulfanyl-2-oxopropanoate + glycine
-
 r
2.6.1.64 S-methyl-L-cysteine + phenylpyruvate
-
 Rattus norvegicus 3-methylsulfanyl-2-oxopropanoate + L-phenylalanine
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.64 dimer 2 * 55000, liver enzyme Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.6.1.64 glutamine transaminase K
-
 Rattus norvegicus
2.6.1.64 glutamine transaminase L
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.15 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.64 60
-
 liver glutaminase transaminase L is unstable at 60°C in absence of an added alpha-keto-acid, kidney glutamine transaminase L is stable Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.64 pyridoxal 5'-phosphate
-
 Rattus norvegicus