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Literature summary extracted from

  • Southern, J.A.; Parker, J.R.; Woods, D.R.
    Novel structure, properties, and inactivation of glutamine synthetase cloned from Bacteroides fragilis (1987), J. Gen. Microbiol., 133, 2437-2446.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expression in Escherichia coli Bacteroides fragilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 (NH4)2SO4 weak, gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 Ala gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 Gly gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 His L-His, gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 L-Arg gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 L-Glu gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 L-Ile weak, gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 L-methionine sulfoximine gamma-glutamyl transferase assay Bacteroides fragilis
6.3.1.2 L-Pro weak, gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 Mg2+ above 0.5 mM, gamma-glutamyl transferase activity Bacteroides fragilis
6.3.1.2 additional information Bacillus fragilis enzyme produced in E. coli is specifically and irreversibly inactivated by Bacillus fragilis cell extract Bacteroides fragilis
6.3.1.2 Snake venom diesterase
-
Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mn2+ MnCl2 stimulates gamma-glutamyl transferase activity of glutamine synthethase from unshocked cells, no effect on the gamma-glutamyl transferase activity of unshocked cells Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 75000
-
6 * 75000, isoform GS2, SDS-PAGE Bacteroides fragilis
6.3.1.2 490000
-
gel filtration Bacteroides fragilis

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Bacteroides fragilis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
Bacteroides fragilis ADP + phosphate + L-Gln
-
?
6.3.1.2 Gln + hydroxylamine + ADP
-
Bacteroides fragilis gamma-Glutamylhydroxamate + NH4+ + ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 hexamer 6 * 75000, isoform GS2, SDS-PAGE Bacteroides fragilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 45
-
gamma-glutamyl transferase assay Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.5 7 gamma-glutamyl transferase assay Bacteroides fragilis