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Literature summary extracted from

  • Winter, H.C.; Dekker, E.E.
    4-Methyleneglutamine amidohydrolase from peanut leaves. Preparation, catalytic properties, and immunological responses of a highly purified form of the enzyme (1991), Plant Physiol., 95, 206-212.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.67 ADP slight activation Arachis hypogaea
3.5.1.67 benzalkonium chloride activation, 126% activity Arachis hypogaea
3.5.1.67 bovine serum albumin activation, 0.5%: 180% activity Arachis hypogaea
3.5.1.67 Detergents ionic or non-ionic detergents, 3-4fold activation Arachis hypogaea
3.5.1.67 methionine sulfoximine very slight activation Arachis hypogaea
3.5.1.67 additional information activation studies Arachis hypogaea
3.5.1.67 additional information no activation by dithiothreitol Arachis hypogaea
3.5.1.67 rabbit preimmune serum activation, 0.5%: 180% activity Arachis hypogaea
3.5.1.67 SDS activation Arachis hypogaea
3.5.1.67 SDS 0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate Arachis hypogaea
3.5.1.67 sodium deoxycholate activation, 160% activity Arachis hypogaea
3.5.1.67 sodium octanoate activation, 130% activity Arachis hypogaea
3.5.1.67 Triton X-100 activation, 180% activity Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.67 4-methylene-L-glutamate slight inhibition Arachis hypogaea
3.5.1.67 4-methylene-L-glutamine competitive, glutamine as substrate, Ki: 30 mM Arachis hypogaea
3.5.1.67 albizziine 3-ureidoalanine, glutamine analog, competitive, 4 mM: 33% inhibition, 10 mM: 50% inhibition, 20 mM: 68% inhibition Arachis hypogaea
3.5.1.67 BO33- strong inhibition, 1 mM: 55% inhibition, 2 mM: 70% inhibition, 5 mM: 87% inhibition Arachis hypogaea
3.5.1.67 diazo-oxo-norleucine very slight inhibition, 4 mM: 7% inhibition Arachis hypogaea
3.5.1.67 L-glutamine competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
3.5.1.67 additional information inhibition studies; no inhibition by methionine sulfoximine, ADP Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.67 additional information
-
 additional information kinetic data Arachis hypogaea
3.5.1.67 5
-
 4-methylene-L-glutamine
-
 Arachis hypogaea
3.5.1.67 20
-
 L-glutamine
-
 Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.67 phosphate slight activation Arachis hypogaea
3.5.1.67 Phosphate esters slight activation Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea highly specific ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the process of N-translocation ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea key evidence in establishing N-translocating role for this nonprotein amino acid ?
-
 ?
3.5.1.67 additional information Arachis hypogaea implicated in the process of N-translocation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.67 Arachis hypogaea
-
 Valencia, cv Tennessee Red
-
3.5.1.67 Arachis hypogaea
-
 two electrophoretically distinct, immunologically identical forms with similar catalytic properties
-
3.5.1.67 Arachis hypogaea
-
 multiple electrophoretically distinct forms
-
3.5.1.67 Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.67 multiple electrophoretically distinct forms Arachis hypogaea
3.5.1.67 two catalytically active forms Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.67 leaf 14- to 16-day germinated peanuts Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.67 349
-
-
 Arachis hypogaea

Storage Stability

EC Number Storage Stability Organism
3.5.1.67 4°C, in solution, stable for many months Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.67 4-methylene-L-glutamine + H2O highly specific Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O highly specific Arachis hypogaea ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O implicated in the process of N-translocation Arachis hypogaea ?
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O key evidence in establishing N-translocating role for this nonprotein amino acid Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamine + H2O
-
 Arachis hypogaea L-glutamate + NH3
-
 ?
3.5.1.67 additional information implicated in the process of N-translocation Arachis hypogaea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.67 More two electrophoretically distinct catalytically active forms: band I: 57000 and 50000 Arachis hypogaea
3.5.1.67 More probably a heterodimer, band II: 50000, probably a homodimer, PAGE Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.67 37
-
 assay at Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.67 additional information
-
 AH-3: pI: 5.1 Arachis hypogaea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.67 30
-
 4-methylene-L-glutamine competitive, glutamine as substrate Arachis hypogaea