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Literature summary extracted from

  • Hell, R.; Bergmann, L.
    gamma-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization (1990), Planta, 180, 603-612.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.2 freezing, -20°C, and thawing results in 70-80% loss of activity Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 Cd2+ 0.2 mM, activity is reduced by 35% Nicotiana tabacum
6.3.2.2 dithioerythritol
-
 Nicotiana tabacum
6.3.2.2 methionine sulfoximine
-
 Nicotiana tabacum
6.3.2.2 methionine sulfoximine
-
 Pisum sativum
6.3.2.2 Zn2+ 0.2 mM, activity is reduced by 19% Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.19
-
 L-Cys
-
 Nicotiana tabacum
6.3.2.2 6.36
-
 2-aminobutyrate
-
 Nicotiana tabacum
6.3.2.2 10.4
-
 2-aminobutyrate L-Glu Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.2 chloroplast
-
 Spinacia oleracea 9507
-
6.3.2.2 chloroplast
-
 Pisum sativum 9507
-
6.3.2.2 cytoplasm
-
 Spinacia oleracea 5737
-
6.3.2.2 cytoplasm
-
 Pisum sativum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+ absolute requirement, maximal activity at 30-50 mM Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 34000
-
 2 * 34000, SDS-PAGE Nicotiana tabacum
6.3.2.2 60000
-
 gel filtration Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Nicotiana tabacum
-
-
-
6.3.2.2 Pisum sativum
-
-
-
6.3.2.2 Spinacia oleracea
-
-
-
6.3.2.2 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 cell suspension culture
-
 Nicotiana tabacum
-

Storage Stability

EC Number Storage Stability Organism
6.3.2.2 4°C, more than 50% loss of activity after 48 h. Stable at -20°C Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + alpha-methyl-L-glutamate + 2-aminobutyrate
-
 Nicotiana tabacum ADP + phosphate + gamma-L-glutamyl-2-aminobutyrate
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Triticum aestivum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Spinacia oleracea ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Pisum sativum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Nicotiana tabacum ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer 2 * 34000, SDS-PAGE Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 8
-
-
 Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.2 7 9 50% of maximal activity at pH 7.0 and pH 9.0 Nicotiana tabacum