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Literature summary extracted from

  • Sekura, R.; Meister, A.
    gamma-Glutamylcysteine synthetase. Further purification, half of the sites" reactivity, subunits, and specificity (1977), J. Biol. Chem., 252, 2599-2605.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 alpha-Methyl-DL-glutamate
-
 Rattus norvegicus
6.3.2.2 beta-Methylglutamate
-
 Rattus norvegicus
6.3.2.2 cis-1-Amino-1,3-dicarboxycyclohexane
-
 Rattus norvegicus
6.3.2.2 D-Glu
-
 Rattus norvegicus
6.3.2.2 DL-2-Amino-4-phosphonobutanoate
-
 Rattus norvegicus
6.3.2.2 DL-alpha-Aminomethylglutarate
-
 Rattus norvegicus
6.3.2.2 DL-Aminoadipate weak Rattus norvegicus
6.3.2.2 gamma-Methylglutamate
-
 Rattus norvegicus
6.3.2.2 L-2-aminohexanedioate i.e. L-alpha-aminoadipate Rattus norvegicus
6.3.2.2 methionine sulfoximine
-
 Rattus norvegicus
6.3.2.2 N-Methyl-L-glutamate
-
 Rattus norvegicus
6.3.2.2 threo-beta-Hydroxy-DL-glutamate
-
 Rattus norvegicus
6.3.2.2 threo-gamma-Hydroxy-L-glutamate
-
 Rattus norvegicus
6.3.2.2 trans-1-Amino-1,3-dicarboxycyclohexane
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 24000
-
 1 * 74000 + 1 * 24000, SDS-PAGE. One enzyme species of MW 100000 Da detected by SDS-PAGE after cross-linking with dimethylsuberimidate Rattus norvegicus
6.3.2.2 74000
-
 1 * 74000 + 1 * 24000, SDS-PAGE. One enzyme species of MW 100000 Da detected by SDS-PAGE after cross-linking with dimethylsuberimidate Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + beta-Glu + L-Cys 17.6% of the activity relative to L-Glu Rattus norvegicus ADP + phosphate + beta-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + N-methyl-L-glutarate + L-Cys
-
 Rattus norvegicus ADP + phosphate + N-methyl-L-glutaryl-L-Cys
-
 ?
6.3.2.2 ATP + threo-beta-hydroxy-L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + threo-beta-hydroxy-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer 1 * 74000 + 1 * 24000, SDS-PAGE. One enzyme species of MW 100000 Da detected by SDS-PAGE after cross-linking with dimethylsuberimidate Rattus norvegicus