Protein Variants | Comment | Organism |
---|---|---|
L75F | L75F mutant is as catalytically efficient as wild-type, athough the NAD Km is slightly elevated compared to wild-type, it is still likely well below the concentration of NAD in the bacterium. IC50 for inhibitors elevated compared to wild-type | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol | - |
Streptococcus pneumoniae | |
(2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol | - |
Streptococcus pneumoniae | |
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine | - |
Streptococcus pneumoniae | |
deoxyribonucleotide | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000028 | - |
deoxyribonucleotide | co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae | |
0.000028 | - |
deoxyribonucleotide | wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | |
0.004 | - |
NAD+ | wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | |
0.026 | - |
NAD+ | mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + deoxyribonucleotide | Streptococcus pneumoniae | - |
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + deoxyribonucleotide | - |
Streptococcus pneumoniae | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent DNA ligase | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
deoxyribonucleotide | co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae | |
0.27 | - |
deoxyribonucleotide | co-substrate: NAD+, wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000051 | - |
deoxyribonucleotide | wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | |
0.000065 | - |
deoxyribonucleotide | co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00004 | - |
wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine | |
0.0001 | - |
wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol | |
0.0002 | - |
wild-type, 25°C, pH 7.5 | Streptococcus pneumoniae | (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol | |
0.01 | - |
mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae | (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol | |
0.012 | - |
mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae | 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine | |
0.03 | - |
mutant L75F, 25°C, pH 7.5 | Streptococcus pneumoniae | (2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol |