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Literature summary for 6.5.1.2 extracted from

  • Singleton, M.R.; Hakansson, K.; Timson, D.J.; Wigley, D.B.
    Structure of the adenylation domain of an NAD+-dependent DNA ligase (1999), Structure, 7, 35-42.
    View publication on PubMed
Search for more literature for 6.5.1.2

Application

Application Comment Organism
medicine potential target for antibiotics due to the fact that eukaryotic cells use ATP instead of NAD+ as cofactor for DNA-ligase Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
1-318 fragment with K114A mutation Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour phase diffusion method, K114A mutant protein fragment Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
K114A prevention of partial adenylation of the enzyme Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Geobacillus stearothermophilus DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
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Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
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 Geobacillus stearothermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Geobacillus stearothermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ required Geobacillus stearothermophilus