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Literature summary for 6.5.1.2 extracted from

  • Lee, J.Y.; Kim, H.K.; Chang, C.; Eom, S.H.; Hwang, K.Y.; Cho, Y.; Yu, Y.G.; Ryu, S.E.; Kwon, S.T.; Suh, S.W.
    Crystallization and preliminary x-ray crystallographic analysis of NAD+-dependent DNA ligase from Thermus filiformis (2000), Acta Crystallogr. Sect. D, D56, 357-358.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL26 Thermus filiformis

Crystallization (Commentary)

Crystallization (Comment) Organism
microseeding technique Thermus filiformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus filiformis DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus filiformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermus filiformis

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Thermus filiformis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermus filiformis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Thermus filiformis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ required Thermus filiformis