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Literature summary for 6.5.1.2 extracted from

  • Luo, J.; Barany, F.
    Identification of essential residues in Thermus thermophilus DNA ligase (1996), Nucleic Acids Res., 24, 3079-3085.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
C412M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C412T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C412V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C415A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C415M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C415T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C415V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C428T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C433A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C433M Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C433T Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
C433V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120G Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120N Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120V Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
D120Y Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
G339A Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
G339D Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
G339E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K118H Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K118L Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K118P1(CCC) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K118P2(CCG) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K118R Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K2942(CTC) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K294L1(CTG) Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K294P Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K294Q Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
K294R Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
R337E Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
R337K Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus
R337Q Site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M. Studies show that residue K118 plays an essential role in the adenylation step, residue D120 may facilitate the deadenylation step, residue G339 and C433 may be involved in formation of the phosphodiester bond Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus thermophilus the enzyme plays a pivotal role in DNA replication, repair and recombination ?
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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HB8, wild-type and site-directed mutants: K118R, K118H, K118L, K118P1(CCC), K118P2(CCG), D120E, D120N, D120Y, D120G, D120A, D120V, K294R, K294Q, K294L1(CTG), K2942(CTC), K294P, K294P, R337K, R337Q, R337E, G339A, G339D, G339E, C412A, C412V, C412T, C412M, C415A, C415V, C415T, C415M, C428A, C428T, C433A, C433V, C433T and C433M
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Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Thermus thermophilus AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme plays a pivotal role in DNA replication, repair and recombination Thermus thermophilus ?
-
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
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Thermus thermophilus