Literature summary for 6.5.1.2 extracted from

Zimmerman, S.B.; Pheiffer, B.H.
Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli (1983), Proc. Natl. Acad. Sci. USA, 80, 5852-5856.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Macromolecular solutes	blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	blunt-end ligation in presence of macromolecular solutes, inactive in absence	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer	Escherichia coli	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	37	activity at 20°C is lower than at 37°C	Escherichia coli

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Release 2023.1 (January 2023)