Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Macromolecular solutes | blunt-end ligation in presence of high concentrations of macromolecular solutes, inactive in absence, e.g. polyethylene glycol 6000, bovine plasma albumin, or Ficoll 70 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | blunt-end ligation in presence of macromolecular solutes, inactive in absence | Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer | Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | activity at 20°C is lower than at 37°C | Escherichia coli |