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Literature summary for 6.5.1.1 extracted from

  • Kiyonari, S.; Kamigochi, T.; Ishino, Y.
    A single amino acid substitution in the DNA-binding domain of Aeropyrum pernix DNA ligase impairs its interaction with proliferating cell nuclear antigen (2007), Extremophiles, 11, 675-684.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeropyrum pernix

Protein Variants

Protein Variants Comment Organism
F115A the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The authors of this reference refer to the 619 amino acid protein containing the mutation at position F132. According to the UniProt numbering the position of this mutation is 115. Surface plasmon resonance analyses reveals that the F132A mutant does not interact with an immobilized subunit of the proliferating cell nuclear antigen (which is known as a DNA sliding clamp that acts as a platform for the assembly of enzymes involved in DNA replication and repair). No stimulation of the ligation activity of the F132A protein by the proliferating cell nuclear antigen can be detected in vitro. These results indicate that the phenylalanine, which is located the predicted proliferating cell nuclear antigen-binding region in the ligase, has a critical role for the physical and functional interaction with proliferating cell nuclear antigen Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YD18 the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein
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Aeropyrum pernix DSM 11879 Q9YD18 the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein
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Purification (Commentary)

Purification (Comment) Organism
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Aeropyrum pernix

Synonyms

Synonyms Comment Organism
APE1094
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Aeropyrum pernix
ApeLig
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Aeropyrum pernix