Literature summary for 6.5.1.1 extracted from

Crut, A.; Nair, P.A.; Koster, D.A.; Shuman, S.; Dekker, N.H.
Dynamics of phosphodiester synthesis by DNA ligase (2008), Proc. Natl. Acad. Sci. USA, 105, 6894-6899.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Chlorella virus

Protein Variants

Protein Variants	Comment	Organism
K27A	the mutant cannot form the covalent ligase-adenylate intermediate and hence cannot form DNA-adenylate, but retains the ability to seal a preadenylylated nick	Chlorella virus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Chlorella virus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Chlorella virus	CVLig relaxes negatively supercoiled plasmid DNA in the presence of 10 mM AMP to generate a mixture of partially relaxed topoisomers, fully relaxed circles, and nicked circular products	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlorella virus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-agarose column chromatography and phosphocellulose column chromatography	Chlorella virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Chlorella virus	AMP + diphosphate + (deoxyribonucleotide)m+n	-	?
additional information	CVLig relaxes negatively supercoiled plasmid DNA in the presence of 10 mM AMP to generate a mixture of partially relaxed topoisomers, fully relaxed circles, and nicked circular products	Chlorella virus	?	-	?

Synonyms

Synonyms	Comment	Organism
CVLig	-	Chlorella virus
DNA ligase	-	Chlorella virus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Chlorella virus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)